BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS00087 (693 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g15690.2 68416.m01989 biotin carboxyl carrier protein of acet... 32 0.41 At1g33020.1 68414.m04066 F-box family protein 30 1.3 At1g52670.1 68414.m05947 biotin/lipoyl attachment domain-contain... 30 1.7 At4g34570.1 68417.m04912 bifunctional dihydrofolate reductase-th... 29 2.9 At5g02180.1 68418.m00139 amino acid transporter family protein b... 29 3.9 At4g02660.1 68417.m00361 WD-40 repeat family protein / beige-rel... 29 3.9 At1g03060.1 68414.m00280 WD-40 repeat family protein / beige-rel... 29 3.9 At5g55220.1 68418.m06883 trigger factor type chaperone family pr... 27 8.9 At1g01510.1 68414.m00067 C-terminal binding protein (ANGUSTIFOLI... 27 8.9 >At3g15690.2 68416.m01989 biotin carboxyl carrier protein of acetyl-CoA carboxylase-related contains weak similarity to Biotin carboxyl carrier protein of acetyl-CoA carboxylase, chloroplast precursor (BCCP) (Swiss-Prot:Q42533) [Arabidopsis thaliana] Length = 263 Score = 31.9 bits (69), Expect = 0.41 Identities = 17/37 (45%), Positives = 22/37 (59%), Gaps = 3/37 (8%) Frame = -3 Query: 193 SDVTGSVAVVHRHDGNPVGY---LLSLAVALTDERKL 92 SDVTG V + R DG PVGY L+S+ + +KL Sbjct: 226 SDVTGEVVKILREDGEPVGYNDALISILPSFPGIKKL 262 >At1g33020.1 68414.m04066 F-box family protein Length = 548 Score = 30.3 bits (65), Expect = 1.3 Identities = 14/36 (38%), Positives = 20/36 (55%) Frame = -1 Query: 579 PCETSRETTSRQTPEPWLSSSCAHLHRVDVVAARNQ 472 P S ET+SR+ + W+S S H+V +A R Q Sbjct: 389 PKPPSTETSSREDHQGWISISSRKYHQVRTIAHRQQ 424 >At1g52670.1 68414.m05947 biotin/lipoyl attachment domain-containing protein similar to SP|Q06881 Biotin carboxyl carrier protein of acetyl-CoA carboxylase (BCCP) {Anabaena sp.}; contains Pfam profile PF00364: Biotin-requiring enzyme Length = 274 Score = 29.9 bits (64), Expect = 1.7 Identities = 13/28 (46%), Positives = 17/28 (60%) Frame = -3 Query: 193 SDVTGSVAVVHRHDGNPVGYLLSLAVAL 110 SDV+G + + R DG PVGY +L L Sbjct: 237 SDVSGEIVKILREDGEPVGYNDALITVL 264 >At4g34570.1 68417.m04912 bifunctional dihydrofolate reductase-thymidylate synthase 2 / DHFR-TS (THY-2) identical to SP|Q05763 Length = 565 Score = 29.1 bits (62), Expect = 2.9 Identities = 14/48 (29%), Positives = 21/48 (43%) Frame = -1 Query: 663 IRSGSNLRRCPCGPSCHNLYANDMAFLMPCETSRETTSRQTPEPWLSS 520 I G LR PSC ++ ++ + C+T T +PW SS Sbjct: 178 IGGGDILREALNKPSCEAIHITEIDTSIDCDTFIPTVDTSAYQPWCSS 225 >At5g02180.1 68418.m00139 amino acid transporter family protein belongs to INTERPRO:IPR002422 amino acid/polyamine transporter, family II Length = 550 Score = 28.7 bits (61), Expect = 3.9 Identities = 11/23 (47%), Positives = 15/23 (65%) Frame = +2 Query: 212 VTISTGPISKRCAAASPGLSTPP 280 +T TG + KRC +SPG+ T P Sbjct: 201 ITCYTGVLMKRCLESSPGIQTYP 223 >At4g02660.1 68417.m00361 WD-40 repeat family protein / beige-related contains Pfam PF00400: WD domain, G-beta repeat; similar to BEIGE (GI:3928547) [Rattus norvegicus]; lysosomal trafficking regulator - Bos taurus, EMBL: AF114785 Length = 3471 Score = 28.7 bits (61), Expect = 3.9 Identities = 15/49 (30%), Positives = 27/49 (55%) Frame = +2 Query: 530 QGSGVCRDVVSRLVSQGIKNAMSFAYKLWHEGPQGHRRRLLPERIPTHT 676 Q +GV D R+V G ++ + +++ +GP+G RR L + + HT Sbjct: 3278 QCAGVSHD--GRIVVTGAEDGLVSVWRVSKDGPRGSRRLRLEKSLCAHT 3324 >At1g03060.1 68414.m00280 WD-40 repeat family protein / beige-related similar to BEIGE (GI:3928547) [Rattus norvegicus]; Similar to gb|U70015 lysosomal trafficking regulator from Mus musculus and contains 2 Pfam PF00400 WD-40, G-beta repeats. ESTs gb|T43386 and gb|AA395236 come from this gene Length = 3601 Score = 28.7 bits (61), Expect = 3.9 Identities = 15/49 (30%), Positives = 27/49 (55%) Frame = +2 Query: 530 QGSGVCRDVVSRLVSQGIKNAMSFAYKLWHEGPQGHRRRLLPERIPTHT 676 Q +GV D R+V G ++ + +++ +GP+G RR L + + HT Sbjct: 3334 QCAGVSHD--GRIVVTGAEDGLVCVWRVSKDGPRGSRRLRLEKALCAHT 3380 >At5g55220.1 68418.m06883 trigger factor type chaperone family protein contains Pfam profiles PF05697: Bacterial trigger factor protein (TF), PF05698: Bacterial trigger factor protein (TF) C-terminus, PF00254: peptidyl-prolyl cis-trans isomerase, FKBP-type Length = 547 Score = 27.5 bits (58), Expect = 8.9 Identities = 14/31 (45%), Positives = 19/31 (61%) Frame = +2 Query: 248 AAASPGLSTPPKDSSLPTDILKILTTFKISS 340 AAASP + T K+ LP D LK+ T + +S Sbjct: 77 AAASPAVETSVKEDKLPAD-LKVTETVQANS 106 >At1g01510.1 68414.m00067 C-terminal binding protein (ANGUSTIFOLIA) nearly identical to C-terminal binding protein ANGUSTIFOLIA [Arabidopsis thaliana] GI:15408535; contains Pfam profile PF02826: D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain Length = 636 Score = 27.5 bits (58), Expect = 8.9 Identities = 16/50 (32%), Positives = 24/50 (48%), Gaps = 3/50 (6%) Frame = +2 Query: 539 GVCRDVVSRLVSQGIKNAMSFAYK---LWHEGPQGHRRRLLPERIPTHTR 679 G+ +V R VS + S A+K L+ + P+G R+ P R P R Sbjct: 163 GMVLGIVGRSVSARYLASRSLAFKMSVLYFDVPEGDEERIRPSRFPRAAR 212 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,123,885 Number of Sequences: 28952 Number of extensions: 265955 Number of successful extensions: 836 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 815 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 836 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1477286152 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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