SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= heS00078
         (707 letters)

Database: uniref50 
           1,657,284 sequences; 575,637,011 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_Q1HPK7 Cluster: SUMO-1 activating enzyme; n=1; Bombyx m...   232   7e-60
UniRef50_Q9UBE0 Cluster: SUMO-activating enzyme subunit 1; n=21;...    85   1e-15
UniRef50_Q9VG70 Cluster: Smt3 activating enzyme 1; n=11; Diptera...    70   5e-11
UniRef50_A7RG90 Cluster: Predicted protein; n=1; Nematostella ve...    58   3e-07
UniRef50_UPI0000585FA6 Cluster: PREDICTED: similar to Aos protei...    57   5e-07
UniRef50_UPI00015B4ED1 Cluster: PREDICTED: similar to ENSANGP000...    56   6e-07
UniRef50_UPI0000D56CB1 Cluster: PREDICTED: similar to ubiquitin-...    51   3e-05
UniRef50_UPI0000DB6D88 Cluster: PREDICTED: similar to Aos1 CG122...    47   4e-04
UniRef50_A7PHI2 Cluster: Chromosome chr17 scaffold_16, whole gen...    42   0.020
UniRef50_Q2AIK6 Cluster: WD40-like Beta Propeller precursor; n=1...    34   3.0  
UniRef50_Q5DAA1 Cluster: SJCHGC02328 protein; n=2; Schistosoma j...    33   5.2  
UniRef50_Q8SG46 Cluster: NADH dehydrogenase subunit 5; n=1; Gast...    33   6.9  
UniRef50_Q6LF29 Cluster: Putative uncharacterized protein; n=2; ...    33   6.9  
UniRef50_Q235X5 Cluster: Putative uncharacterized protein; n=1; ...    33   6.9  
UniRef50_A3QN80 Cluster: NADH dehydrogenase subunit 5; n=1; Euch...    33   9.1  

>UniRef50_Q1HPK7 Cluster: SUMO-1 activating enzyme; n=1; Bombyx
           mori|Rep: SUMO-1 activating enzyme - Bombyx mori (Silk
           moth)
          Length = 339

 Score =  232 bits (567), Expect = 7e-60
 Identities = 109/124 (87%), Positives = 109/124 (87%)
 Frame = +1

Query: 1   ADWNSPEMRSRLRRGDCGYFAMKLLLRFRDEYNRNPDPARRKDDINLLLKMRDEIVRELS 180
           ADWNSPEMRSRLRRGDCGYFAMKLLLRFRDEYNRNPDPARRKDDINLLLKMRDEIVRELS
Sbjct: 216 ADWNSPEMRSRLRRGDCGYFAMKLLLRFRDEYNRNPDPARRKDDINLLLKMRDEIVRELS 275

Query: 181 LPASFIKDSLFYDVFXXXXXXXXXXXXXXXQEVVKAVSQREPPHNNMFFFNPIKCLGYTE 360
           LPASFIKDSLFYDVF               QEVVKAVSQREPPHNNMFFFNPIKCLGYTE
Sbjct: 276 LPASFIKDSLFYDVFGVVAAAAAVIGGVIGQEVVKAVSQREPPHNNMFFFNPIKCLGYTE 335

Query: 361 LYGQ 372
           LYGQ
Sbjct: 336 LYGQ 339


>UniRef50_Q9UBE0 Cluster: SUMO-activating enzyme subunit 1; n=21;
           Euteleostomi|Rep: SUMO-activating enzyme subunit 1 -
           Homo sapiens (Human)
          Length = 346

 Score = 85.4 bits (202), Expect = 1e-15
 Identities = 40/122 (32%), Positives = 67/122 (54%)
 Frame = +1

Query: 4   DWNSPEMRSRLRRGDCGYFAMKLLLRFRDEYNRNPDPARRKDDINLLLKMRDEIVRELSL 183
           DW+S + ++ L+R    YF +++LL+FR +  R+P     ++D  LLL++R++++  L +
Sbjct: 223 DWSSEKAKAALKRTTSDYFLLQVLLKFRTDKGRDPSSDTYEEDSELLLQIRNDVLDSLGI 282

Query: 184 PASFIKDSLFYDVFXXXXXXXXXXXXXXXQEVVKAVSQREPPHNNMFFFNPIKCLGYTEL 363
               + +      F               QE+VKA+SQR+PPHNN FFF+ +K  G  E 
Sbjct: 283 SPDLLPEDFVRYCFSEMAPVCAVVGGILAQEIVKALSQRDPPHNNFFFFDGMKGNGIVEC 342

Query: 364 YG 369
            G
Sbjct: 343 LG 344


>UniRef50_Q9VG70 Cluster: Smt3 activating enzyme 1; n=11;
           Diptera|Rep: Smt3 activating enzyme 1 - Drosophila
           melanogaster (Fruit fly)
          Length = 337

 Score = 70.1 bits (164), Expect = 5e-11
 Identities = 39/122 (31%), Positives = 57/122 (46%)
 Frame = +1

Query: 4   DWNSPEMRSRLRRGDCGYFAMKLLLRFRDEYNRNPDPARRKDDINLLLKMRDEIVRELSL 183
           D   P    +L+R   G   + +L +FR  + R+P    R+ D+ LL  +RDE+     L
Sbjct: 219 DVTEPSYLRKLKRNGPGVLLLSVLQKFRTTHKRDPSYKTREADLELLRGIRDEL-----L 273

Query: 184 PASFIKDSLFYDVFXXXXXXXXXXXXXXXQEVVKAVSQREPPHNNMFFFNPIKCLGYTEL 363
           P S + D     +F               QEV+K V++ E PH N+F F+P  C GY E 
Sbjct: 274 PNSILGDEALGLIFAQISPAVAVVGGVVAQEVIKVVTKLEAPHRNLFVFDPETCAGYVEA 333

Query: 364 YG 369
            G
Sbjct: 334 IG 335


>UniRef50_A7RG90 Cluster: Predicted protein; n=1; Nematostella
           vectensis|Rep: Predicted protein - Nematostella
           vectensis
          Length = 342

 Score = 57.6 bits (133), Expect = 3e-07
 Identities = 31/111 (27%), Positives = 54/111 (48%)
 Frame = +1

Query: 34  LRRGDCGYFAMKLLLRFRDEYNRNPDPARRKDDINLLLKMRDEIVRELSLPASFIKDSLF 213
           L+R    YF ++++LRFR ++ R PD  +R  D   L  ++ E++ +L++    I     
Sbjct: 231 LKRLPSVYFILQVILRFRAKHGRAPDSLQRTSDEKELNCLKQEVMSDLNISQDLIDQDFA 290

Query: 214 YDVFXXXXXXXXXXXXXXXQEVVKAVSQREPPHNNMFFFNPIKCLGYTELY 366
                              QE+VKAVS ++ P NN FF++ ++  G  E +
Sbjct: 291 CHCLSELSPVCAIVGGVVGQEIVKAVSGKDAPLNNFFFYDGLEGHGMVECF 341


>UniRef50_UPI0000585FA6 Cluster: PREDICTED: similar to Aos protein;
           n=1; Strongylocentrotus purpuratus|Rep: PREDICTED:
           similar to Aos protein - Strongylocentrotus purpuratus
          Length = 338

 Score = 56.8 bits (131), Expect = 5e-07
 Identities = 30/119 (25%), Positives = 57/119 (47%)
 Frame = +1

Query: 4   DWNSPEMRSRLRRGDCGYFAMKLLLRFRDEYNRNPDPARRKDDINLLLKMRDEIVRELSL 183
           DW+S   + +L+R    +F +++L +F D++ R P+ A  ++D   L ++ +E+  EL L
Sbjct: 216 DWSSLTEK-QLKRAPYVFFILRVLFKFHDQFGRKPEAASSENDSTELQRLHNEVFTELGL 274

Query: 184 PASFIKDSLFYDVFXXXXXXXXXXXXXXXQEVVKAVSQREPPHNNMFFFNPIKCLGYTE 360
               I  +                     QE++KA S R+ P +N F ++ +  LG  +
Sbjct: 275 KNDLIDTNYTEYCAGELGPTCAIVGGIIGQEIIKAASGRDAPLDNFFLYDGVDSLGMVD 333


>UniRef50_UPI00015B4ED1 Cluster: PREDICTED: similar to
           ENSANGP00000023276; n=1; Nasonia vitripennis|Rep:
           PREDICTED: similar to ENSANGP00000023276 - Nasonia
           vitripennis
          Length = 330

 Score = 56.4 bits (130), Expect = 6e-07
 Identities = 31/94 (32%), Positives = 49/94 (52%)
 Frame = +1

Query: 55  YFAMKLLLRFRDEYNRNPDPARRKDDINLLLKMRDEIVRELSLPASFIKDSLFYDVFXXX 234
           Y+ M +LL FR+++ R+P P+ R    N+L      I+ + +L    +   L  D++   
Sbjct: 229 YYLMLILLNFREKHKRDPLPSERST--NILNDEAKAIIEKYNL-GDKMDHLLNGDLYAQI 285

Query: 235 XXXXXXXXXXXXQEVVKAVSQREPPHNNMFFFNP 336
                       QE++K VSQ+E PHNN+F FNP
Sbjct: 286 SPVCAIVGGVMGQEIIKTVSQKERPHNNLFIFNP 319


>UniRef50_UPI0000D56CB1 Cluster: PREDICTED: similar to
           ubiquitin-like 1 (sentrin) activating enzyme E1A
           (predicted); n=1; Tribolium castaneum|Rep: PREDICTED:
           similar to ubiquitin-like 1 (sentrin) activating enzyme
           E1A (predicted) - Tribolium castaneum
          Length = 333

 Score = 50.8 bits (116), Expect = 3e-05
 Identities = 30/99 (30%), Positives = 46/99 (46%)
 Frame = +1

Query: 55  YFAMKLLLRFRDEYNRNPDPARRKDDINLLLKMRDEIVRELSLPASFIKDSLFYDVFXXX 234
           +  M +LL FR  + R+PD   + D+I  L  +  EI++        + D+L   VF   
Sbjct: 229 FVCMLMLLEFRKRFERDPDLENKSDEIEELRDIATEIIKLYQFTKVNV-DNLLDLVFGEV 287

Query: 235 XXXXXXXXXXXXQEVVKAVSQREPPHNNMFFFNPIKCLG 351
                       QEV+KA S +E   NN+F F+P+   G
Sbjct: 288 SPVCAILGGIMSQEVIKAASHKEVTINNIFLFDPVTYSG 326


>UniRef50_UPI0000DB6D88 Cluster: PREDICTED: similar to Aos1
           CG12276-PA; n=1; Apis mellifera|Rep: PREDICTED: similar
           to Aos1 CG12276-PA - Apis mellifera
          Length = 287

 Score = 47.2 bits (107), Expect = 4e-04
 Identities = 28/102 (27%), Positives = 48/102 (47%), Gaps = 1/102 (0%)
 Frame = +1

Query: 70  LLLRFRDEYNRNPDPARRKDDINLLLKMR-DEIVRELSLPASFIKDSLFYDVFXXXXXXX 246
           ++L +R++Y ++P P+ R  +     K+    I+++  L    I   +  D++       
Sbjct: 190 VMLNYREKYGKDPSPSERGSE---KFKVEASAIIKKFDLENK-INHLVEGDIYAQVSPVC 245

Query: 247 XXXXXXXXQEVVKAVSQREPPHNNMFFFNPIKCLGYTELYGQ 372
                   QE++K VSQ+  PHNN+F FNP    G     GQ
Sbjct: 246 AIVGGIMAQEIIKTVSQKGAPHNNLFLFNPDTLCGKILRLGQ 287


>UniRef50_A7PHI2 Cluster: Chromosome chr17 scaffold_16, whole genome
           shotgun sequence; n=1; Vitis vinifera|Rep: Chromosome
           chr17 scaffold_16, whole genome shotgun sequence - Vitis
           vinifera (Grape)
          Length = 323

 Score = 41.5 bits (93), Expect = 0.020
 Identities = 30/105 (28%), Positives = 46/105 (43%), Gaps = 3/105 (2%)
 Frame = +1

Query: 55  YFAMKLLLRFRDEYNRNPDPARRKDDINLLLKMRDEIVRELSLPASFIKDSLFYDVFXXX 234
           Y AM+++ RF +   RNP      D +  +LK++ E+    S   S + D+L   +    
Sbjct: 211 YLAMRVIERFEEAEGRNPGEISIAD-LPGVLKLKKELCEAQSFNESHVPDALLERLVSDT 269

Query: 235 XXXXXXXXXXXX---QEVVKAVSQREPPHNNMFFFNPIKCLGYTE 360
                          QEV+KA+S +  P  N FFF+ I   G  E
Sbjct: 270 SEFPPVCAILGGILGQEVIKAISGKGDPLKNFFFFDAIDGKGIIE 314


>UniRef50_Q2AIK6 Cluster: WD40-like Beta Propeller precursor; n=1;
           Halothermothrix orenii H 168|Rep: WD40-like Beta
           Propeller precursor - Halothermothrix orenii H 168
          Length = 918

 Score = 34.3 bits (75), Expect = 3.0
 Identities = 24/69 (34%), Positives = 35/69 (50%), Gaps = 5/69 (7%)
 Frame = -3

Query: 420 KPIFVIQYSCQ--TDLISLTIQFGIT*AFDWIEEEHI-VMWWFPLTDG--FDHLLANNST 256
           +P F  QYS Q  T L+SLT    +    DW+ E ++ +M  +PL +   + HLL  NS 
Sbjct: 661 EPSFDFQYSYQPGTFLLSLTTNQSLIKDGDWVRERNVDIMLGYPLINKSFYYHLLLLNSV 720

Query: 255 DNSSSCCHN 229
            +   C  N
Sbjct: 721 YHDEYCYDN 729


>UniRef50_Q5DAA1 Cluster: SJCHGC02328 protein; n=2; Schistosoma
           japonicum|Rep: SJCHGC02328 protein - Schistosoma
           japonicum (Blood fluke)
          Length = 355

 Score = 33.5 bits (73), Expect = 5.2
 Identities = 10/25 (40%), Positives = 19/25 (76%)
 Frame = +1

Query: 271 QEVVKAVSQREPPHNNMFFFNPIKC 345
           QE+++A++++  PH N +FFN  +C
Sbjct: 314 QEIIRAITRKGAPHGNWYFFNGSQC 338


>UniRef50_Q8SG46 Cluster: NADH dehydrogenase subunit 5; n=1;
           Gasterophilus sp. LRC-2002|Rep: NADH dehydrogenase
           subunit 5 - Gasterophilus sp. LRC-2002
          Length = 166

 Score = 33.1 bits (72), Expect = 6.9
 Identities = 17/29 (58%), Positives = 19/29 (65%)
 Frame = +2

Query: 587 FINTSSLF*YSKIGFLNTGNMWYMPYIKT 673
           F N  SLF YS   FL  G+MW+MPYI T
Sbjct: 68  FFN-KSLFCYSSTNFL--GSMWFMPYIST 93


>UniRef50_Q6LF29 Cluster: Putative uncharacterized protein; n=2;
           Plasmodium|Rep: Putative uncharacterized protein -
           Plasmodium falciparum (isolate 3D7)
          Length = 1025

 Score = 33.1 bits (72), Expect = 6.9
 Identities = 18/51 (35%), Positives = 24/51 (47%)
 Frame = +2

Query: 533 NCNKPMLCLTLNDYGQISFINTSSLF*YSKIGFLNTGNMWYMPYIKTLTYL 685
           N N   LC  L  Y  +  I+ S L   SK   LN GN+ Y+  +  L +L
Sbjct: 275 NMNNSTLCYILYSYSTLFTISNSYLLYISKYILLNPGNLNYLDILSLLYFL 325


>UniRef50_Q235X5 Cluster: Putative uncharacterized protein; n=1;
           Tetrahymena thermophila SB210|Rep: Putative
           uncharacterized protein - Tetrahymena thermophila SB210
          Length = 2084

 Score = 33.1 bits (72), Expect = 6.9
 Identities = 21/70 (30%), Positives = 34/70 (48%), Gaps = 1/70 (1%)
 Frame = -1

Query: 212 NNESLMKLAGS-DNSRTISSRIFSSKLMSSFLRAGSGFLLYSSLKRNRSFIAK*PQSPRR 36
           NN SL        NS  IS   F   L  + +RA S  L+Y   ++ R F    P++ + 
Sbjct: 749 NNNSLKHTYNQIKNSNKISENNFEKNLEDN-IRAHSKKLIYQKYQKKREFNPSTPENRQS 807

Query: 35  SLERISGEFQ 6
           +++ ++ EFQ
Sbjct: 808 TMQSVNDEFQ 817


>UniRef50_A3QN80 Cluster: NADH dehydrogenase subunit 5; n=1;
           Euchorthippus pulvinatus|Rep: NADH dehydrogenase subunit
           5 - Euchorthippus pulvinatus
          Length = 134

 Score = 32.7 bits (71), Expect = 9.1
 Identities = 18/33 (54%), Positives = 23/33 (69%), Gaps = 2/33 (6%)
 Frame = +2

Query: 581 ISFINTSS-LF*YSKIGFLN-TGNMWYMPYIKT 673
           IS IN SS LF  S   F++ +G+MW+MPYI T
Sbjct: 92  ISNINFSSDLFSLSMFSFISFSGSMWFMPYIST 124


  Database: uniref50
    Posted date:  Oct 5, 2007 11:19 AM
  Number of letters in database: 575,637,011
  Number of sequences in database:  1,657,284
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 642,682,170
Number of Sequences: 1657284
Number of extensions: 11881209
Number of successful extensions: 25165
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 24432
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 25156
length of database: 575,637,011
effective HSP length: 98
effective length of database: 413,223,179
effective search space used: 56611575523
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -