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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= heS00078
         (707 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g50680.1 68418.m06280 SUMO activating enzyme 1b (SAE1b) ident...    33   0.14 
At5g50580.2 68418.m06266 SUMO activating enzyme, putative nearly...    33   0.14 
At5g50580.1 68418.m06265 SUMO activating enzyme, putative nearly...    33   0.14 
At4g24940.1 68417.m03572 SUMO activating enzyme 1a (SAE1a) ident...    29   4.0  
At4g09660.1 68417.m01586 hypothetical protein                          28   7.0  
At5g47190.1 68418.m05819 ribosomal protein L19 family protein si...    27   9.2  
At3g20490.1 68416.m02595 expressed protein                             27   9.2  

>At5g50680.1 68418.m06280 SUMO activating enzyme 1b (SAE1b)
           identical to SUMO activating enzyme 1b [Arabidopsis
           thaliana] GI:22652852; nearly identical to At5g50580;
           contains Pfam profile PF00899: ThiF family
          Length = 320

 Score = 33.5 bits (73), Expect = 0.14
 Identities = 24/96 (25%), Positives = 41/96 (42%), Gaps = 3/96 (3%)
 Frame = +1

Query: 55  YFAMKLLLRFRDEYNRNPDPARRKDDINLLLKMRDEIVRELSLPASFIKDSLFYDVFXXX 234
           YFAM+++  F +   R P      D +  +LK++ E+    S+  + I D L   +    
Sbjct: 211 YFAMRVIELFEETEGRKPGECSLSD-LPRVLKLKKELCEGNSVSENHIPDILLERLVSNN 269

Query: 235 XXXXXXXXXXXX---QEVVKAVSQREPPHNNMFFFN 333
                          QEV+K +S +  P  N F+F+
Sbjct: 270 TEFPPACAIIGGILGQEVIKVISGKGEPLKNFFYFD 305


>At5g50580.2 68418.m06266 SUMO activating enzyme, putative nearly
           identical to SUMO activating enzyme 1b [Arabidopsis
           thaliana] GI:22652852; nearly identical to At5g50680;
           contains Pfam profile PF00899: ThiF family
          Length = 320

 Score = 33.5 bits (73), Expect = 0.14
 Identities = 24/96 (25%), Positives = 41/96 (42%), Gaps = 3/96 (3%)
 Frame = +1

Query: 55  YFAMKLLLRFRDEYNRNPDPARRKDDINLLLKMRDEIVRELSLPASFIKDSLFYDVFXXX 234
           YFAM+++  F +   R P      D +  +LK++ E+    S+  + I D L   +    
Sbjct: 211 YFAMRVIELFEETEGRKPGECSLSD-LPRVLKLKKELCEGNSVSENHIPDILLERLVSNN 269

Query: 235 XXXXXXXXXXXX---QEVVKAVSQREPPHNNMFFFN 333
                          QEV+K +S +  P  N F+F+
Sbjct: 270 TEFPPACAIIGGILGQEVIKVISGKGEPLKNFFYFD 305


>At5g50580.1 68418.m06265 SUMO activating enzyme, putative nearly
           identical to SUMO activating enzyme 1b [Arabidopsis
           thaliana] GI:22652852; nearly identical to At5g50680;
           contains Pfam profile PF00899: ThiF family
          Length = 318

 Score = 33.5 bits (73), Expect = 0.14
 Identities = 24/96 (25%), Positives = 41/96 (42%), Gaps = 3/96 (3%)
 Frame = +1

Query: 55  YFAMKLLLRFRDEYNRNPDPARRKDDINLLLKMRDEIVRELSLPASFIKDSLFYDVFXXX 234
           YFAM+++  F +   R P      D +  +LK++ E+    S+  + I D L   +    
Sbjct: 209 YFAMRVIELFEETEGRKPGECSLSD-LPRVLKLKKELCEGNSVSENHIPDILLERLVSNN 267

Query: 235 XXXXXXXXXXXX---QEVVKAVSQREPPHNNMFFFN 333
                          QEV+K +S +  P  N F+F+
Sbjct: 268 TEFPPACAIIGGILGQEVIKVISGKGEPLKNFFYFD 303


>At4g24940.1 68417.m03572 SUMO activating enzyme 1a (SAE1a)
           identical to SUMO activating enzyme 1a [Arabidopsis
           thaliana] GI:22652850; contains Pfam profile PF00899:
           ThiF family
          Length = 322

 Score = 28.7 bits (61), Expect = 4.0
 Identities = 23/96 (23%), Positives = 41/96 (42%), Gaps = 3/96 (3%)
 Frame = +1

Query: 55  YFAMKLLLRFRDEYNRNPDPARRKDDINLLLKMRDEIVRELSLPASFIKDSLFYDVFXXX 234
           YFAM+++  F +   R        D +  +L+++ ++    S+  S I D L   +    
Sbjct: 211 YFAMRVIEVFEESEGRKHGECSLLD-LARVLEIKKQLCEANSVSESHIPDILLERLITGT 269

Query: 235 XXXXXXXXXXXX---QEVVKAVSQREPPHNNMFFFN 333
                          QEV+KAVS +  P  N F+++
Sbjct: 270 TEFPPVCAIVGGILAQEVIKAVSGKGDPLKNFFYYD 305


>At4g09660.1 68417.m01586 hypothetical protein
          Length = 664

 Score = 27.9 bits (59), Expect = 7.0
 Identities = 13/28 (46%), Positives = 19/28 (67%), Gaps = 1/28 (3%)
 Frame = +1

Query: 94  YNRNP-DPARRKDDINLLLKMRDEIVRE 174
           +N+ P DPA+RK  ++  L  RDE+ RE
Sbjct: 17  FNKLPSDPAKRKSILSYHLNQRDEVRRE 44


>At5g47190.1 68418.m05819 ribosomal protein L19 family protein
           similar to plastid ribosomal protein L19 precursor
           [Spinacia oleracea] gi|7582403|gb|AAF64312
          Length = 229

 Score = 27.5 bits (58), Expect = 9.2
 Identities = 21/61 (34%), Positives = 31/61 (50%)
 Frame = -1

Query: 203 SLMKLAGSDNSRTISSRIFSSKLMSSFLRAGSGFLLYSSLKRNRSFIAK*PQSPRRSLER 24
           S++  A S NSR   SR+F +   S+F     GF + S  K+ + FIAK  +S     E 
Sbjct: 30  SILPRASSVNSRLSVSRVFLNHSSSNF-----GFAIDS--KKRKEFIAKAEESTEGETEA 82

Query: 23  I 21
           +
Sbjct: 83  V 83


>At3g20490.1 68416.m02595 expressed protein
          Length = 458

 Score = 27.5 bits (58), Expect = 9.2
 Identities = 12/35 (34%), Positives = 20/35 (57%)
 Frame = +1

Query: 103 NPDPARRKDDINLLLKMRDEIVRELSLPASFIKDS 207
           NP+    KDD ++ ++ RD+ + E S P  F  D+
Sbjct: 74  NPNKCSVKDDRSVAVEDRDDDIEEFSSPEDFPTDA 108


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,187,224
Number of Sequences: 28952
Number of extensions: 267195
Number of successful extensions: 534
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 526
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 534
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1526202912
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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