Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00027
(706 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g57320.1 68418.m07160 villin, putative similar to villin 2 (V... 69 4e-12
At3g57410.1 68416.m06391 villin 3 (VLN3) nearly identical to vil... 62 3e-10
At2g41740.1 68415.m05159 villin 2 (VLN2) nearly identical to vil... 62 3e-10
At4g30160.1 68417.m04289 villin, putative similar to villin 2 (... 61 8e-10
At2g29890.1 68415.m03630 villin 1 (VLN1) nearly identical to vil... 37 0.011
At5g45810.1 68418.m05633 CBL-interacting protein kinase 19 (CIPK... 30 1.3
At2g05920.1 68415.m00642 subtilase family protein contains simil... 28 5.2
At1g54860.1 68414.m06263 expressed protein 28 5.2
At5g44750.1 68418.m05485 UMUC-like DNA repair family protein / B... 28 6.9
At2g36470.1 68415.m04476 expressed protein 28 6.9
At1g18265.1 68414.m02278 expressed protein 28 6.9
At2g47980.1 68415.m06004 expressed protein 27 9.2
>At5g57320.1 68418.m07160 villin, putative similar to villin 2
(VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
(VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
profiles PF00626: Gelsolin repeat, PF02209: Villin
headpiece domain
Length = 962
Score = 68.5 bits (160), Expect = 4e-12
Identities = 27/59 (45%), Positives = 42/59 (71%)
Frame = +2
Query: 239 EGHRKIYKGDSYIILRTTSDSRNNLSWDIHYWIGRESTQDESGAAAISTLAWTTSSGAQ 415
E H K + GDSYI+L+TT+ +L DIHYW+G++S+QDE+GA A+ T+ ++ G +
Sbjct: 37 ESHGKFFTGDSYIVLKTTASRSGSLHHDIHYWLGKDSSQDEAGAVAVMTVELDSALGGR 95
Score = 62.1 bits (144), Expect = 3e-10
Identities = 53/187 (28%), Positives = 82/187 (43%), Gaps = 6/187 (3%)
Frame = +3
Query: 162 AFANVGRTAGVQIWRIQNFEPIPVAQKDIGKS-TKEIHTSFYGRPRTVATIYHGIST-TG 335
A G+ +G++IWRI+NF+P+ V Q+ GK T + + +++H I G
Sbjct: 11 ALQGAGQKSGIEIWRIENFKPVTVPQESHGKFFTGDSYIVLKTTASRSGSLHHDIHYWLG 70
Query: 336 SVGSRRKTSQEPPQFQRWP----GRQVRGHSGPAPRDHGSRKCFVPIIFPDTLDLFGGRE 503
S+ + GR V+ F P I P GG
Sbjct: 71 KDSSQDEAGAVAVMTVELDSALGGRAVQYREVQGHETEKFLSYFKPCIIPQE----GGVA 126
Query: 504 SVGFNHVVTNAGAQKRMFQVKGKRDVRVRQVDPQIASMNKGDCFVLSLDNDIYVFVGEKA 683
S GFNHV Q R++ KGK VRV++V +++N D F+L ++ I+ F G K+
Sbjct: 127 S-GFNHVKPEEH-QTRLYICKGKHVVRVKEVPFVRSTLNHEDVFILDTESKIFQFSGSKS 184
Query: 684 KNVEKLK 704
E+ K
Sbjct: 185 SIQERAK 191
Score = 44.8 bits (101), Expect = 6e-05
Identities = 21/39 (53%), Positives = 25/39 (64%)
Frame = +1
Query: 385 VGLDDKFGGTAVQHRETMGHESALFLSYFQTPLTYLEGG 501
V LD GG AVQ+RE GHE+ FLSYF+ + EGG
Sbjct: 86 VELDSALGGRAVQYREVQGHETEKFLSYFKPCIIPQEGG 124
>At3g57410.1 68416.m06391 villin 3 (VLN3) nearly identical to villin
3 (VLN3) [Arabidopsis thaliana] GI:3415117
Length = 965
Score = 62.1 bits (144), Expect = 3e-10
Identities = 24/57 (42%), Positives = 39/57 (68%)
Frame = +2
Query: 245 HRKIYKGDSYIILRTTSDSRNNLSWDIHYWIGRESTQDESGAAAISTLAWTTSSGAQ 415
H K Y GD+YI+L+TT + +DIH+WIG++++QDE+G AA+ T+ + G +
Sbjct: 39 HGKFYMGDTYIVLQTTQNKGGAYLFDIHFWIGKDTSQDEAGTAAVKTVELDAALGGR 95
Score = 59.7 bits (138), Expect = 2e-09
Identities = 55/188 (29%), Positives = 83/188 (44%), Gaps = 6/188 (3%)
Frame = +3
Query: 159 PAFANVGRTAGVQIWRIQNFEPIPVAQKDIGK-----STKEIHTSFYGRPRTVATIYHGI 323
PAF VG+ G +IWRI+NFEP+PV + + GK + + T+ + I+ I
Sbjct: 10 PAFQGVGQKPGTEIWRIENFEPVPVPKSEHGKFYMGDTYIVLQTTQNKGGAYLFDIHFWI 69
Query: 324 STTGSVGSRRKTSQEPPQFQ-RWPGRQVRGHSGPAPRDHGSRKCFVPIIFPDTLDLFGGR 500
S + + + GR V+ + H S K F+ P + L GG
Sbjct: 70 GKDTSQDEAGTAAVKTVELDAALGGRAVQYRE---IQGHESDK-FLSYFKPCIIPLEGGV 125
Query: 501 ESVGFNHVVTNAGAQKRMFQVKGKRDVRVRQVDPQIASMNKGDCFVLSLDNDIYVFVGEK 680
S GF + R++ KGKR V ++QV +S+N D F+L IY F G
Sbjct: 126 AS-GFKK-PEEEEFETRLYTCKGKRAVHLKQVPFARSSLNHDDVFILDTKEKIYQFNGAN 183
Query: 681 AKNVEKLK 704
+ E+ K
Sbjct: 184 SNIQERAK 191
Score = 46.8 bits (106), Expect = 1e-05
Identities = 23/39 (58%), Positives = 26/39 (66%)
Frame = +1
Query: 385 VGLDDKFGGTAVQHRETMGHESALFLSYFQTPLTYLEGG 501
V LD GG AVQ+RE GHES FLSYF+ + LEGG
Sbjct: 86 VELDAALGGRAVQYREIQGHESDKFLSYFKPCIIPLEGG 124
Score = 32.7 bits (71), Expect = 0.24
Identities = 17/65 (26%), Positives = 28/65 (43%), Gaps = 1/65 (1%)
Frame = +2
Query: 212 ELRTNTRCSEGH-RKIYKGDSYIILRTTSDSRNNLSWDIHYWIGRESTQDESGAAAISTL 388
+ + T S+ H K+Y GD Y++L T + + W G+ S Q++ A
Sbjct: 400 DANSKTVLSKDHVGKLYSGDCYLVLYTYHSGERKEDYFLCCWFGKNSNQEDQETAVRLAS 459
Query: 389 AWTTS 403
T S
Sbjct: 460 TMTNS 464
Score = 27.5 bits (58), Expect = 9.2
Identities = 11/44 (25%), Positives = 24/44 (54%)
Frame = +3
Query: 567 GKRDVRVRQVDPQIASMNKGDCFVLSLDNDIYVFVGEKAKNVEK 698
G + + QV+ S+N DCF+L ++++VG + + ++
Sbjct: 529 GVHNNKALQVEAVATSLNSYDCFLLQSGTSMFLWVGNHSTHEQQ 572
>At2g41740.1 68415.m05159 villin 2 (VLN2) nearly identical to villin
2 (VLN2) [Arabidopsis thaliana] GI:3415115
Length = 976
Score = 62.1 bits (144), Expect = 3e-10
Identities = 53/187 (28%), Positives = 83/187 (44%), Gaps = 5/187 (2%)
Frame = +3
Query: 159 PAFANVGRTAGVQIWRIQNFEPIPVAQKDIGK-----STKEIHTSFYGRPRTVATIYHGI 323
PAF G+ G +IWRI+NFE +PV + + GK + + T+ + I+ I
Sbjct: 8 PAFQGAGQKPGTEIWRIENFEAVPVPKSEHGKFYMGDTYIVLQTTQNKGGAYLFDIHFWI 67
Query: 324 STTGSVGSRRKTSQEPPQFQRWPGRQVRGHSGPAPRDHGSRKCFVPIIFPDTLDLFGGRE 503
S + + + G + H + H S K F+ P + L GG
Sbjct: 68 GKDTSQDEAGTAAVKTVELDAVLGGRAVQHR--EIQGHESDK-FLSYFKPCIIPLEGGVA 124
Query: 504 SVGFNHVVTNAGAQKRMFQVKGKRDVRVRQVDPQIASMNKGDCFVLSLDNDIYVFVGEKA 683
S GF V + R++ KGKR +R++QV +S+N D F+L + IY F G +
Sbjct: 125 S-GFKTVEEEV-FETRLYTCKGKRAIRLKQVPFARSSLNHDDVFILDTEEKIYQFNGANS 182
Query: 684 KNVEKLK 704
E+ K
Sbjct: 183 NIQERAK 189
Score = 61.3 bits (142), Expect = 6e-10
Identities = 23/48 (47%), Positives = 36/48 (75%)
Frame = +2
Query: 245 HRKIYKGDSYIILRTTSDSRNNLSWDIHYWIGRESTQDESGAAAISTL 388
H K Y GD+YI+L+TT + +DIH+WIG++++QDE+G AA+ T+
Sbjct: 37 HGKFYMGDTYIVLQTTQNKGGAYLFDIHFWIGKDTSQDEAGTAAVKTV 84
Score = 49.2 bits (112), Expect = 3e-06
Identities = 26/46 (56%), Positives = 28/46 (60%)
Frame = +1
Query: 385 VGLDDKFGGTAVQHRETMGHESALFLSYFQTPLTYLEGGNPSVLTT 522
V LD GG AVQHRE GHES FLSYF+ + LEGG S T
Sbjct: 84 VELDAVLGGRAVQHREIQGHESDKFLSYFKPCIIPLEGGVASGFKT 129
Score = 29.9 bits (64), Expect = 1.7
Identities = 11/41 (26%), Positives = 20/41 (48%)
Frame = +2
Query: 251 KIYKGDSYIILRTTSDSRNNLSWDIHYWIGRESTQDESGAA 373
K+Y GD Y++L T + + W G++S ++ A
Sbjct: 412 KLYSGDCYLVLYTYHSGERKDEYFLSCWFGKKSIPEDQDTA 452
>At4g30160.1 68417.m04289 villin, putative similar to villin 2
(VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
(VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
profiles PF00626: Gelsolin repeat, PF02209: Villin
headpiece domain
Length = 974
Score = 60.9 bits (141), Expect = 8e-10
Identities = 52/188 (27%), Positives = 79/188 (42%), Gaps = 6/188 (3%)
Frame = +3
Query: 159 PAFANVGRTAGVQIWRIQNFEPIPVAQKDIGK-----STKEIHTSFYGRPRTVATIYHGI 323
PAF G+ AG++IWRI+NF P P+ + IGK S + T+ I++ +
Sbjct: 10 PAFQGAGQKAGIEIWRIENFIPTPIPKSSIGKFFTGDSYIVLKTTALKTGALRHDIHYWL 69
Query: 324 STTGSVGSRRKTSQEPPQFQ-RWPGRQVRGHSGPAPRDHGSRKCFVPIIFPDTLDLFGGR 500
S + + + GR V+ F P I P GG
Sbjct: 70 GKDTSQDEAGTAAVKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIPQE----GGV 125
Query: 501 ESVGFNHVVTNAGAQKRMFQVKGKRDVRVRQVDPQIASMNKGDCFVLSLDNDIYVFVGEK 680
S GF HVV R+F +GK V V++V +S+N D ++L + I+ F G
Sbjct: 126 AS-GFKHVVAEEHI-TRLFVCRGKHVVHVKEVPFARSSLNHDDIYILDTKSKIFQFNGSN 183
Query: 681 AKNVEKLK 704
+ E+ K
Sbjct: 184 SSIQERAK 191
Score = 60.5 bits (140), Expect = 1e-09
Identities = 24/55 (43%), Positives = 38/55 (69%)
Frame = +2
Query: 251 KIYKGDSYIILRTTSDSRNNLSWDIHYWIGRESTQDESGAAAISTLAWTTSSGAQ 415
K + GDSYI+L+TT+ L DIHYW+G++++QDE+G AA+ T+ + G +
Sbjct: 41 KFFTGDSYIVLKTTALKTGALRHDIHYWLGKDTSQDEAGTAAVKTVELDAALGGR 95
Score = 44.0 bits (99), Expect = 1e-04
Identities = 21/39 (53%), Positives = 25/39 (64%)
Frame = +1
Query: 385 VGLDDKFGGTAVQHRETMGHESALFLSYFQTPLTYLEGG 501
V LD GG AVQ+RE GHE+ FLSYF+ + EGG
Sbjct: 86 VELDAALGGRAVQYREVQGHETEKFLSYFKPCIIPQEGG 124
Score = 29.5 bits (63), Expect = 2.3
Identities = 12/39 (30%), Positives = 22/39 (56%)
Frame = +3
Query: 558 QVKGKRDVRVRQVDPQIASMNKGDCFVLSLDNDIYVFVG 674
Q G +++ QVDP AS+N ++L D+ ++ + G
Sbjct: 525 QGSGPENMQAIQVDPVAASLNSSYYYILHNDSSVFTWAG 563
>At2g29890.1 68415.m03630 villin 1 (VLN1) nearly identical to villin
1 (VLN1) [Arabidopsis thaliana] GI:3415113
Length = 909
Score = 37.1 bits (82), Expect = 0.011
Identities = 15/46 (32%), Positives = 28/46 (60%)
Frame = +2
Query: 251 KIYKGDSYIILRTTSDSRNNLSWDIHYWIGRESTQDESGAAAISTL 388
K + G++Y++LRT + +DIHYW+G ++ + +S A+ L
Sbjct: 41 KFHSGNAYLVLRTFLRKIESPQYDIHYWLGIDANEVDSILASDKAL 86
Score = 35.9 bits (79), Expect = 0.026
Identities = 21/54 (38%), Positives = 29/54 (53%), Gaps = 2/54 (3%)
Frame = +1
Query: 343 GVDARRVRS--RRNFNVGLDDKFGGTAVQHRETMGHESALFLSYFQTPLTYLEG 498
G+DA V S + + LD G VQ+RE G E+ FLSYF+ + +EG
Sbjct: 70 GIDANEVDSILASDKALDLDAALGCCTVQYREVQGQETEKFLSYFKPCIIPVEG 123
Score = 34.7 bits (76), Expect = 0.060
Identities = 17/54 (31%), Positives = 29/54 (53%)
Frame = +3
Query: 543 QKRMFQVKGKRDVRVRQVDPQIASMNKGDCFVLSLDNDIYVFVGEKAKNVEKLK 704
Q + + KG VRV++V +S+N D F+L + +++F G + EK K
Sbjct: 137 QVTLLRCKGDHVVRVKEVPFLRSSLNHDDVFILDTASKVFLFAGCNSSTQEKAK 190
>At5g45810.1 68418.m05633 CBL-interacting protein kinase 19 (CIPK19)
identical to CBL-interacting protein kinase 19
[Arabidopsis thaliana] gi|14009296|gb|AAK50347
Length = 483
Score = 30.3 bits (65), Expect = 1.3
Identities = 24/64 (37%), Positives = 28/64 (43%)
Frame = +3
Query: 321 ISTTGSVGSRRKTSQEPPQFQRWPGRQVRGHSGPAPRDHGSRKCFVPIIFPDTLDLFGGR 500
I + SV R T EP F+ + GR+ RG S P P S F I F DL G
Sbjct: 308 IESIESVSGRSSTVSEPEDFESFDGRR-RGGSMPRP---ASLNAFDLISFSPGFDLSGLF 363
Query: 501 ESVG 512
E G
Sbjct: 364 EDDG 367
>At2g05920.1 68415.m00642 subtilase family protein contains
similarity to cucumisin-like serine protease GI:3176874
from [Arabidopsis thaliana]
Length = 754
Score = 28.3 bits (60), Expect = 5.2
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = +3
Query: 468 FPDTLDLFGGRESVGFNHVVTNAGAQKRMFQV 563
+P LFGG+ V + VTN GA +++V
Sbjct: 654 YPSFSVLFGGKRVVRYTREVTNVGAASSVYKV 685
>At1g54860.1 68414.m06263 expressed protein
Length = 200
Score = 28.3 bits (60), Expect = 5.2
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = -1
Query: 208 ILHICTPAVLPTLAKAGWTLALSRAFLSDSDVIG 107
IL +C P +PTLA +T +L+DS +G
Sbjct: 113 ILPVCIPNRIPTLALTNYTQTGYARYLNDSRYVG 146
>At5g44750.1 68418.m05485 UMUC-like DNA repair family protein / BRCT
domain-containing protein low similarity to Rev1S [Homo
sapiens] GI:12483635; contains Pfam profiles PF00817:
ImpB/MucB/SamB family, PF00533: BRCA1 C Terminus (BRCT)
domain
Length = 1102
Score = 27.9 bits (59), Expect = 6.9
Identities = 15/53 (28%), Positives = 28/53 (52%)
Frame = -3
Query: 635 EAVAFVHRSYLRIDLSNADISLPFDLEHSFLGSRVRDDVVKTDGFPPSK*VKG 477
E + ++ S L D + D+S D+E L S +R+++++T G S + G
Sbjct: 460 ECLLYIVTSALSCDEAFLDVSDLSDVETEVLASTIRNEILETTGCSASAGIGG 512
>At2g36470.1 68415.m04476 expressed protein
Length = 327
Score = 27.9 bits (59), Expect = 6.9
Identities = 13/40 (32%), Positives = 24/40 (60%)
Frame = +2
Query: 308 NLSWDIHYWIGRESTQDESGAAAISTLAWTTSSGAQRSST 427
++ WD+H W +E+ S +++ T A ++SS + SST
Sbjct: 238 DMMWDLHDWFYKETLSSVSTSSSSKT-ASSSSSSSTSSST 276
>At1g18265.1 68414.m02278 expressed protein
Length = 280
Score = 27.9 bits (59), Expect = 6.9
Identities = 17/50 (34%), Positives = 22/50 (44%), Gaps = 1/50 (2%)
Frame = +2
Query: 176 RQNSRCANMENTELRTNTRCSEGHRKIYKGDSY-IILRTTSDSRNNLSWD 322
R++ C N N N S+ H IIL +SDS N LSW+
Sbjct: 83 REHKDCNNNNNNNNNNNGFISKSHLADGLDQKKPIILHWSSDSTNRLSWE 132
>At2g47980.1 68415.m06004 expressed protein
Length = 1098
Score = 27.5 bits (58), Expect = 9.2
Identities = 15/58 (25%), Positives = 26/58 (44%)
Frame = +3
Query: 450 CFVPIIFPDTLDLFGGRESVGFNHVVTNAGAQKRMFQVKGKRDVRVRQVDPQIASMNK 623
C P ++ T L G + F V G+Q+ Q + + + R P++ S+NK
Sbjct: 194 CTPPRVYRQTATLMGLQLVTSFISVANTLGSQRETTQRQLNAESKKRADGPRVDSLNK 251
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,498,266
Number of Sequences: 28952
Number of extensions: 387691
Number of successful extensions: 1140
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1083
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1140
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1516419560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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