Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= heS00026
         (728 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC6B1.07 |prp1|zer1|U4/U6 x U5 tri-snRNP complex subunit Prp1|...    79   8e-16
SPCC1393.07c |mug4||sequence orphan|Schizosaccharomyces pombe|ch...    28   1.2  

>SPBC6B1.07 |prp1|zer1|U4/U6 x U5 tri-snRNP complex subunit
           Prp1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 906

 Score = 78.6 bits (185), Expect = 8e-16
 Identities = 36/73 (49%), Positives = 50/73 (68%)
 Frame = +2

Query: 509 KGCEVNPSSEELWLEAARLQPIDTARAVIAHAARNLPHSVRIWVKAADLEQEAKAKRRVF 688
           KGCE    SE++WLEA RL P   A+ +IA+A + LP SV +W++A  LE +A+ K+R+ 
Sbjct: 303 KGCENCSRSEDVWLEAIRLHPAAEAKVIIANAVKKLPKSVTLWLEAEKLENQAQHKKRII 362

Query: 689 RKALEHIPNSVRL 727
           +KALE  P SV L
Sbjct: 363 KKALEFNPTSVSL 375



 Score = 49.6 bits (113), Expect = 4e-07
 Identities = 28/71 (39%), Positives = 39/71 (54%), Gaps = 1/71 (1%)
 Frame = +2

Query: 509 KGCEVNPSSEELWLEAARL-QPIDTARAVIAHAARNLPHSVRIWVKAADLEQEAKAKRRV 685
           K  E NP+S  LW EA  L + +D AR ++A A   +P S+ +W+  A LE    AK +V
Sbjct: 364 KALEFNPTSVSLWKEAVNLEEEVDNARILLARAVELIPMSIDLWLALARLETYENAK-KV 422

Query: 686 FRKALEHIPNS 718
             KA + I  S
Sbjct: 423 LNKARQTIRTS 433



 Score = 44.4 bits (100), Expect = 2e-05
 Identities = 25/68 (36%), Positives = 37/68 (54%), Gaps = 1/68 (1%)
 Frame = +2

Query: 527 PSSEELWLEAARLQ-PIDTARAVIAHAARNLPHSVRIWVKAADLEQEAKAKRRVFRKALE 703
           P S  LWLEA +L+      + +I  A    P SV +W +A +LE+E    R +  +A+E
Sbjct: 339 PKSVTLWLEAEKLENQAQHKKRIIKKALEFNPTSVSLWKEAVNLEEEVDNARILLARAVE 398

Query: 704 HIPNSVRL 727
            IP S+ L
Sbjct: 399 LIPMSIDL 406



 Score = 41.1 bits (92), Expect = 2e-04
 Identities = 20/59 (33%), Positives = 33/59 (55%)
 Frame = +2

Query: 509 KGCEVNPSSEELWLEAARLQPIDTARAVIAHAARNLPHSVRIWVKAADLEQEAKAKRRV 685
           +  E+ P S +LWL  ARL+  + A+ V+  A + +  S  +W+ AA LE++     RV
Sbjct: 395 RAVELIPMSIDLWLALARLETYENAKKVLNKARQTIRTSHEVWIAAARLEEQQGNVSRV 453



 Score = 38.3 bits (85), Expect = 0.001
 Identities = 23/74 (31%), Positives = 37/74 (50%), Gaps = 4/74 (5%)
 Frame = +2

Query: 518 EVNPSSEELWLEAARLQPI----DTARAVIAHAARNLPHSVRIWVKAADLEQEAKAKRRV 685
           E NP     W+ AARL+ +      A+++I     N   S  +W++A  L   A+AK  +
Sbjct: 272 ETNPKHASGWVAAARLEEVANKLSQAQSLILKGCENCSRSEDVWLEAIRLHPAAEAK-VI 330

Query: 686 FRKALEHIPNSVRL 727
              A++ +P SV L
Sbjct: 331 IANAVKKLPKSVTL 344



 Score = 37.9 bits (84), Expect = 0.001
 Identities = 21/57 (36%), Positives = 30/57 (52%), Gaps = 4/57 (7%)
 Frame = +2

Query: 524 NPSSEELWLEAARLQ----PIDTARAVIAHAARNLPHSVRIWVKAADLEQEAKAKRR 682
           NP +E LWLE  +++     I   RA +A A +  P S  +W +A  LE  A+ K R
Sbjct: 742 NPKNEFLWLELIKMELRAGNISQVRAALAKALQECPSSGLLWTEAIWLEPRAQRKTR 798



 Score = 35.9 bits (79), Expect = 0.006
 Identities = 25/74 (33%), Positives = 42/74 (56%), Gaps = 4/74 (5%)
 Frame = +2

Query: 518 EVNPSSEELWLEAARLQPI----DTARAVIAHAARNLPHSVRIWVKAADLEQEAKAKRRV 685
           E N +SEE+WL A R++ +    + AR ++A  AR    + RIW K+  LE+    K R 
Sbjct: 606 EYNSNSEEIWLAAVRIEFVNNENERARKLLAR-ARIESGTERIWTKSISLERILDEKDR- 663

Query: 686 FRKALEHIPNSVRL 727
              AL+ + N++++
Sbjct: 664 ---ALQLLENALKI 674



 Score = 34.7 bits (76), Expect = 0.014
 Identities = 18/52 (34%), Positives = 30/52 (57%), Gaps = 3/52 (5%)
 Frame = +2

Query: 572 IDTARAVIAHAARNLPHSVRIWVKAADLEQ---EAKAKRRVFRKALEHIPNS 718
           ID ARAV A + R  P S ++W++A +LE+     ++   +  KA+E  P +
Sbjct: 526 IDCARAVFAFSLRVYPKSEKLWLRAVELEKLYGTTESVCSILEKAVESCPKA 577



 Score = 31.9 bits (69), Expect = 0.096
 Identities = 12/23 (52%), Positives = 19/23 (82%)
 Frame = +3

Query: 444 WIASARLEEVTGKVQTARNLIIR 512
           W+A+ARLEEV  K+  A++LI++
Sbjct: 281 WVAAARLEEVANKLSQAQSLILK 303



 Score = 30.7 bits (66), Expect = 0.22
 Identities = 13/30 (43%), Positives = 18/30 (60%)
 Frame = +3

Query: 444 WIASARLEEVTGKVQTARNLIIRDVKSIRA 533
           WIA+ARLEE  G V     ++ R V  ++A
Sbjct: 437 WIAAARLEEQQGNVSRVEKIMARGVSELQA 466


>SPCC1393.07c |mug4||sequence orphan|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 845

 Score = 28.3 bits (60), Expect = 1.2
 Identities = 14/27 (51%), Positives = 16/27 (59%)
 Frame = +2

Query: 320 ILQHRRPRLPLMQLEAPCSFNFCSLRA 400
           I Q     LP + L +PCSF  CSLRA
Sbjct: 48  IAQKSNISLPFLTL-SPCSFTICSLRA 73


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,606,859
Number of Sequences: 5004
Number of extensions: 47883
Number of successful extensions: 122
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 106
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 121
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 343230174
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)