Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00026
(728 letters)
Database: fruitfly
53,049 sequences; 24,988,368 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BT021415-1|AAX33563.1| 931|Drosophila melanogaster LD04472p pro... 126 3e-29
AE014296-3081|AAF49211.2| 931|Drosophila melanogaster CG6841-PA... 126 3e-29
>BT021415-1|AAX33563.1| 931|Drosophila melanogaster LD04472p
protein.
Length = 931
Score = 126 bits (304), Expect = 3e-29
Identities = 59/73 (80%), Positives = 66/73 (90%)
Frame = +2
Query: 509 KGCEVNPSSEELWLEAARLQPIDTARAVIAHAARNLPHSVRIWVKAADLEQEAKAKRRVF 688
+GCE+N SE+LWLEAARLQP DTA+AVIA AAR++P SVRIW+KAADLE E KAKRRVF
Sbjct: 324 RGCEMNIQSEDLWLEAARLQPPDTAKAVIAQAARHIPTSVRIWIKAADLESETKAKRRVF 383
Query: 689 RKALEHIPNSVRL 727
RKALEHIPNSVRL
Sbjct: 384 RKALEHIPNSVRL 396
Score = 46.0 bits (104), Expect = 5e-05
Identities = 21/24 (87%), Positives = 23/24 (95%)
Frame = +3
Query: 441 SWIASARLEEVTGKVQTARNLIIR 512
+WIASARLEEVTGKVQ ARNLI+R
Sbjct: 301 AWIASARLEEVTGKVQMARNLIMR 324
Score = 44.0 bits (99), Expect = 2e-04
Identities = 24/66 (36%), Positives = 35/66 (53%)
Frame = +2
Query: 506 NKGCEVNPSSEELWLEAARLQPIDTARAVIAHAARNLPHSVRIWVKAADLEQEAKAKRRV 685
++ E +S ELWL ARL+ + AR V+ A N+P +IW AA LE EA +
Sbjct: 414 SRAVECCNTSVELWLALARLETYENARKVLNKARENIPTDRQIWTTAAKLE-EANGNIHM 472
Query: 686 FRKALE 703
K ++
Sbjct: 473 VEKIID 478
Score = 43.6 bits (98), Expect = 3e-04
Identities = 26/68 (38%), Positives = 35/68 (51%)
Frame = +2
Query: 509 KGCEVNPSSEELWLEAARLQPIDTARAVIAHAARNLPHSVRIWVKAADLEQEAKAKRRVF 688
K E P+S LW A L+ D AR +++ A SV +W+ A LE A R+V
Sbjct: 385 KALEHIPNSVRLWKAAVELENPDDARILLSRAVECCNTSVELWLALARLETYENA-RKVL 443
Query: 689 RKALEHIP 712
KA E+IP
Sbjct: 444 NKARENIP 451
Score = 41.1 bits (92), Expect = 0.002
Identities = 25/68 (36%), Positives = 38/68 (55%), Gaps = 1/68 (1%)
Frame = +2
Query: 527 PSSEELWLEAARLQPIDTA-RAVIAHAARNLPHSVRIWVKAADLEQEAKAKRRVFRKALE 703
P+S +W++AA L+ A R V A ++P+SVR+W A +LE A R + +A+E
Sbjct: 360 PTSVRIWIKAADLESETKAKRRVFRKALEHIPNSVRLWKAAVELENPDDA-RILLSRAVE 418
Query: 704 HIPNSVRL 727
SV L
Sbjct: 419 CCNTSVEL 426
Score = 40.3 bits (90), Expect = 0.003
Identities = 25/74 (33%), Positives = 37/74 (50%), Gaps = 4/74 (5%)
Frame = +2
Query: 518 EVNPSSEELWLEAARLQ----PIDTARAVIAHAARNLPHSVRIWVKAADLEQEAKAKRRV 685
E NP+ W+ +ARL+ + AR +I S +W++AA L+ AK V
Sbjct: 293 ETNPNHPPAWIASARLEEVTGKVQMARNLIMRGCEMNIQSEDLWLEAARLQPPDTAK-AV 351
Query: 686 FRKALEHIPNSVRL 727
+A HIP SVR+
Sbjct: 352 IAQAARHIPTSVRI 365
Score = 35.1 bits (77), Expect = 0.099
Identities = 23/77 (29%), Positives = 44/77 (57%), Gaps = 7/77 (9%)
Frame = +2
Query: 518 EVNPSSEELWLEAARLQ----PIDTARAVIAHAARNLPHSVRIWVKAADLE---QEAKAK 676
+ NP+SE++WL A +L+ + AR ++A A + P + R+ +K+A LE ++
Sbjct: 626 QANPNSEDIWLAAVKLESENSEYERARRLLAKARGSAP-TPRVMMKSARLEWALEKFDEA 684
Query: 677 RRVFRKALEHIPNSVRL 727
R+ +A+E P+ +L
Sbjct: 685 LRLLEEAVEVFPDFPKL 701
Score = 33.5 bits (73), Expect = 0.30
Identities = 18/57 (31%), Positives = 30/57 (52%), Gaps = 3/57 (5%)
Frame = +2
Query: 557 ARLQPIDTARAVIAHAARNLPHSVRIWVKAADLEQE---AKAKRRVFRKALEHIPNS 718
A+ + ARAV AHA + P IW++AA E+ ++ + ++A+ H P S
Sbjct: 541 AKENAFECARAVYAHALQIFPSKKSIWLRAAYFEKNHGTRESLEALLQRAVAHCPKS 597
Score = 32.7 bits (71), Expect = 0.53
Identities = 20/59 (33%), Positives = 29/59 (49%), Gaps = 4/59 (6%)
Frame = +2
Query: 527 PSSEELWLEAARLQ----PIDTARAVIAHAARNLPHSVRIWVKAADLEQEAKAKRRVFR 691
P SE LWL A+ + + AR +++ A + P+S IW+ A LE E R R
Sbjct: 595 PKSEILWLMGAKSKWMAGDVPAARGILSLAFQANPNSEDIWLAAVKLESENSEYERARR 653
Score = 31.5 bits (68), Expect = 1.2
Identities = 20/70 (28%), Positives = 35/70 (50%), Gaps = 6/70 (8%)
Frame = +2
Query: 524 NPSSEELWLEAARLQ----PIDTARAVIAHAARNLPHSVRIWVKAADLEQEAKAKRRVF- 688
NP LWLEA R++ + A ++A A + P++ +W +A +E + + K +
Sbjct: 763 NPKVAVLWLEAIRVELRAGLKEIASTMMARALQECPNAGELWAEAIFMETKPQRKTKSVD 822
Query: 689 -RKALEHIPN 715
K EH P+
Sbjct: 823 ALKKCEHDPH 832
>AE014296-3081|AAF49211.2| 931|Drosophila melanogaster CG6841-PA
protein.
Length = 931
Score = 126 bits (304), Expect = 3e-29
Identities = 59/73 (80%), Positives = 66/73 (90%)
Frame = +2
Query: 509 KGCEVNPSSEELWLEAARLQPIDTARAVIAHAARNLPHSVRIWVKAADLEQEAKAKRRVF 688
+GCE+N SE+LWLEAARLQP DTA+AVIA AAR++P SVRIW+KAADLE E KAKRRVF
Sbjct: 324 RGCEMNIQSEDLWLEAARLQPPDTAKAVIAQAARHIPTSVRIWIKAADLESETKAKRRVF 383
Query: 689 RKALEHIPNSVRL 727
RKALEHIPNSVRL
Sbjct: 384 RKALEHIPNSVRL 396
Score = 46.0 bits (104), Expect = 5e-05
Identities = 21/24 (87%), Positives = 23/24 (95%)
Frame = +3
Query: 441 SWIASARLEEVTGKVQTARNLIIR 512
+WIASARLEEVTGKVQ ARNLI+R
Sbjct: 301 AWIASARLEEVTGKVQMARNLIMR 324
Score = 44.0 bits (99), Expect = 2e-04
Identities = 24/66 (36%), Positives = 35/66 (53%)
Frame = +2
Query: 506 NKGCEVNPSSEELWLEAARLQPIDTARAVIAHAARNLPHSVRIWVKAADLEQEAKAKRRV 685
++ E +S ELWL ARL+ + AR V+ A N+P +IW AA LE EA +
Sbjct: 414 SRAVECCNTSVELWLALARLETYENARKVLNKARENIPTDRQIWTTAAKLE-EANGNIHM 472
Query: 686 FRKALE 703
K ++
Sbjct: 473 VEKIID 478
Score = 43.6 bits (98), Expect = 3e-04
Identities = 26/68 (38%), Positives = 35/68 (51%)
Frame = +2
Query: 509 KGCEVNPSSEELWLEAARLQPIDTARAVIAHAARNLPHSVRIWVKAADLEQEAKAKRRVF 688
K E P+S LW A L+ D AR +++ A SV +W+ A LE A R+V
Sbjct: 385 KALEHIPNSVRLWKAAVELENPDDARILLSRAVECCNTSVELWLALARLETYENA-RKVL 443
Query: 689 RKALEHIP 712
KA E+IP
Sbjct: 444 NKARENIP 451
Score = 41.1 bits (92), Expect = 0.002
Identities = 25/68 (36%), Positives = 38/68 (55%), Gaps = 1/68 (1%)
Frame = +2
Query: 527 PSSEELWLEAARLQPIDTA-RAVIAHAARNLPHSVRIWVKAADLEQEAKAKRRVFRKALE 703
P+S +W++AA L+ A R V A ++P+SVR+W A +LE A R + +A+E
Sbjct: 360 PTSVRIWIKAADLESETKAKRRVFRKALEHIPNSVRLWKAAVELENPDDA-RILLSRAVE 418
Query: 704 HIPNSVRL 727
SV L
Sbjct: 419 CCNTSVEL 426
Score = 40.3 bits (90), Expect = 0.003
Identities = 25/74 (33%), Positives = 37/74 (50%), Gaps = 4/74 (5%)
Frame = +2
Query: 518 EVNPSSEELWLEAARLQ----PIDTARAVIAHAARNLPHSVRIWVKAADLEQEAKAKRRV 685
E NP+ W+ +ARL+ + AR +I S +W++AA L+ AK V
Sbjct: 293 ETNPNHPPAWIASARLEEVTGKVQMARNLIMRGCEMNIQSEDLWLEAARLQPPDTAK-AV 351
Query: 686 FRKALEHIPNSVRL 727
+A HIP SVR+
Sbjct: 352 IAQAARHIPTSVRI 365
Score = 35.1 bits (77), Expect = 0.099
Identities = 23/77 (29%), Positives = 44/77 (57%), Gaps = 7/77 (9%)
Frame = +2
Query: 518 EVNPSSEELWLEAARLQ----PIDTARAVIAHAARNLPHSVRIWVKAADLE---QEAKAK 676
+ NP+SE++WL A +L+ + AR ++A A + P + R+ +K+A LE ++
Sbjct: 626 QANPNSEDIWLAAVKLESENSEYERARRLLAKARGSAP-TPRVMMKSARLEWALEKFDEA 684
Query: 677 RRVFRKALEHIPNSVRL 727
R+ +A+E P+ +L
Sbjct: 685 LRLLEEAVEVFPDFPKL 701
Score = 33.5 bits (73), Expect = 0.30
Identities = 18/57 (31%), Positives = 30/57 (52%), Gaps = 3/57 (5%)
Frame = +2
Query: 557 ARLQPIDTARAVIAHAARNLPHSVRIWVKAADLEQE---AKAKRRVFRKALEHIPNS 718
A+ + ARAV AHA + P IW++AA E+ ++ + ++A+ H P S
Sbjct: 541 AKENAFECARAVYAHALQIFPSKKSIWLRAAYFEKNHGTRESLEALLQRAVAHCPKS 597
Score = 32.7 bits (71), Expect = 0.53
Identities = 20/59 (33%), Positives = 29/59 (49%), Gaps = 4/59 (6%)
Frame = +2
Query: 527 PSSEELWLEAARLQ----PIDTARAVIAHAARNLPHSVRIWVKAADLEQEAKAKRRVFR 691
P SE LWL A+ + + AR +++ A + P+S IW+ A LE E R R
Sbjct: 595 PKSEILWLMGAKSKWMAGDVPAARGILSLAFQANPNSEDIWLAAVKLESENSEYERARR 653
Score = 31.5 bits (68), Expect = 1.2
Identities = 20/70 (28%), Positives = 35/70 (50%), Gaps = 6/70 (8%)
Frame = +2
Query: 524 NPSSEELWLEAARLQ----PIDTARAVIAHAARNLPHSVRIWVKAADLEQEAKAKRRVF- 688
NP LWLEA R++ + A ++A A + P++ +W +A +E + + K +
Sbjct: 763 NPKVAVLWLEAIRVELRAGLKEIASTMMARALQECPNAGELWAEAIFMETKPQRKTKSVD 822
Query: 689 -RKALEHIPN 715
K EH P+
Sbjct: 823 ALKKCEHDPH 832
Database: fruitfly
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 24,988,368
Number of sequences in database: 53,049
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 29,914,026
Number of Sequences: 53049
Number of extensions: 591300
Number of successful extensions: 1424
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 1354
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1422
length of database: 24,988,368
effective HSP length: 83
effective length of database: 20,585,301
effective search space used: 3273062859
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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