BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS00020 (703 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC22H12.04c |rps102|rps1-2, rps3a-2|40S ribosomal protein S3a|... 104 1e-23 SPAC13G6.02c |rps101|rps1-1, rps3a-1|40S ribosomal protein S3a|S... 103 2e-23 SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharo... 33 0.030 SPBC1826.01c |mot1||TATA-binding protein associated factor Mot1|... 31 0.12 SPAC1834.08 |mak1|phk3|histidine kinase Mak1|Schizosaccharomyces... 28 1.5 SPBC337.13c |gtr1||Gtr1/RagA G protein Gtr1|Schizosaccharomyces ... 27 3.4 SPBC21C3.15c |||aldehyde dehydrogenase |Schizosaccharomyces pomb... 27 3.4 SPCC24B10.11c |||THO complex subunit 7 |Schizosaccharomyces pomb... 27 3.4 SPAC6F12.11c |sfc1||transcription factor TFIIIC complex A box as... 26 4.5 SPBC1921.05 |ape2||aminopeptidase Ape2|Schizosaccharomyces pombe... 25 7.9 SPAC664.08c |||traub transciption factor family protein |Schizos... 25 7.9 >SPAC22H12.04c |rps102|rps1-2, rps3a-2|40S ribosomal protein S3a|Schizosaccharomyces pombe|chr 1|||Manual Length = 252 Score = 104 bits (250), Expect = 1e-23 Identities = 46/90 (51%), Positives = 70/90 (77%) Frame = +3 Query: 243 FPQIRLIAEYVQGRNVLCNFHGMDLTTDKLRWMVKKWQTLIEANIDVKTTDGYVLRVFCI 422 F +++L E +QG++ L +F+G+ +T+DKLR +V+KWQT IEA+ +KTTDGY+ RVF I Sbjct: 81 FRKVKLRVEDIQGKSCLTSFNGLSITSDKLRSLVRKWQTTIEADQTIKTTDGYLCRVFVI 140 Query: 423 GFTNKDSLSQRKTCYAQHTQVRAIRKKMWK 512 GFT + + +KT YAQ +Q+RAIR+KM++ Sbjct: 141 GFTRRRANQVKKTTYAQSSQIRAIRQKMFQ 170 Score = 89.8 bits (213), Expect = 3e-19 Identities = 41/67 (61%), Positives = 52/67 (77%) Frame = +1 Query: 55 IVDPFTRKDWYDVKAPSMFSKRQVGTTLVNRTQGTKIASEGLKGRVFEVSLADLQADTDA 234 +VDPF+RK+WYD+KAP+ F + VG TLVNRT G K A++ LKGR+ EVSLADLQ D Sbjct: 20 VVDPFSRKEWYDIKAPAFFEVKNVGKTLVNRTAGLKNANDSLKGRILEVSLADLQ--KDE 77 Query: 235 ERSFRKL 255 E +FRK+ Sbjct: 78 EHAFRKV 84 Score = 40.7 bits (91), Expect = 2e-04 Identities = 17/31 (54%), Positives = 24/31 (77%) Frame = +1 Query: 610 IYPLRDVCNPKVKVLKRPRFEISKLMELHGE 702 I+PL++V KVK+LK P+ + KL+ELHGE Sbjct: 204 IFPLQNVLVRKVKILKAPKHDAQKLLELHGE 234 Score = 33.1 bits (72), Expect = 0.040 Identities = 16/52 (30%), Positives = 29/52 (55%) Frame = +2 Query: 509 EIITRDVTNSELREVVNKLIPDSIAKDIEKAAMASTLCAMFAIRK*KC*RGP 664 ++I ++ +RE+V KLIP+ I ++IE+A + +RK K + P Sbjct: 170 QVIQNQTSSCSMRELVQKLIPEVIGREIERATGSIFPLQNVLVRKVKILKAP 221 >SPAC13G6.02c |rps101|rps1-1, rps3a-1|40S ribosomal protein S3a|Schizosaccharomyces pombe|chr 1|||Manual Length = 252 Score = 103 bits (247), Expect = 2e-23 Identities = 45/90 (50%), Positives = 69/90 (76%) Frame = +3 Query: 243 FPQIRLIAEYVQGRNVLCNFHGMDLTTDKLRWMVKKWQTLIEANIDVKTTDGYVLRVFCI 422 F +++L E +QG++ L +F+G D+T+DKLR +V+KWQ+ IEAN +KTTDGY+ R+F I Sbjct: 81 FRKVKLRVEDIQGKSCLTSFNGFDMTSDKLRSLVRKWQSTIEANQTIKTTDGYLCRIFVI 140 Query: 423 GFTNKDSLSQRKTCYAQHTQVRAIRKKMWK 512 GFT++ +KT YAQ +Q+RAI +KM++ Sbjct: 141 GFTSRRVNQVKKTTYAQSSQIRAIHQKMFQ 170 Score = 92.7 bits (220), Expect = 5e-20 Identities = 43/67 (64%), Positives = 52/67 (77%) Frame = +1 Query: 55 IVDPFTRKDWYDVKAPSMFSKRQVGTTLVNRTQGTKIASEGLKGRVFEVSLADLQADTDA 234 +VDPF+RKDWYD+KAP+ F + VG TLVNRT G K A++ LKGR+ EVSLADLQ D Sbjct: 20 VVDPFSRKDWYDIKAPAFFEVKNVGKTLVNRTAGLKNANDSLKGRILEVSLADLQ--KDE 77 Query: 235 ERSFRKL 255 E SFRK+ Sbjct: 78 EHSFRKV 84 Score = 42.3 bits (95), Expect = 6e-05 Identities = 18/33 (54%), Positives = 25/33 (75%) Frame = +1 Query: 604 HGIYPLRDVCNPKVKVLKRPRFEISKLMELHGE 702 + IYPL++V KVK+LK P+ + KL+ELHGE Sbjct: 202 NNIYPLQNVFVRKVKILKAPKHDAQKLLELHGE 234 Score = 29.5 bits (63), Expect = 0.49 Identities = 12/31 (38%), Positives = 19/31 (61%) Frame = +2 Query: 509 EIITRDVTNSELREVVNKLIPDSIAKDIEKA 601 ++I ++E+V KLIP+ I + IEKA Sbjct: 170 QVIQNQANGCSMKELVQKLIPEVIGRAIEKA 200 >SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharomyces pombe|chr 2|||Manual Length = 1309 Score = 33.5 bits (73), Expect = 0.030 Identities = 16/52 (30%), Positives = 26/52 (50%), Gaps = 2/52 (3%) Frame = +2 Query: 416 LHWFHQ*GLLEPTQDVLRPAHSGQ--SNQKENVEIITRDVTNSELREVVNKL 565 LHW+ L+ +Q + P H+GQ S + NVE + V E +++ L Sbjct: 1162 LHWYSLMSLMGTSQGICIPRHAGQVLSTPRRNVEFVNERVPTPEFSSLLSSL 1213 >SPBC1826.01c |mot1||TATA-binding protein associated factor Mot1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1953 Score = 31.5 bits (68), Expect = 0.12 Identities = 21/52 (40%), Positives = 26/52 (50%) Frame = -1 Query: 688 PSTSRSRNGASSTLSLSDCKHRAEGRCHGSLLDVLGNGVRNQLVHHLPEFRV 533 PSTSR RN S LS S KHRA+ GS + + N V + E R+ Sbjct: 736 PSTSRERNNNISELSNSRTKHRAKDDPKGSFCFSVDEPMLNGDVEFVGEERM 787 >SPAC1834.08 |mak1|phk3|histidine kinase Mak1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1639 Score = 27.9 bits (59), Expect = 1.5 Identities = 14/40 (35%), Positives = 20/40 (50%) Frame = -1 Query: 658 SSTLSLSDCKHRAEGRCHGSLLDVLGNGVRNQLVHHLPEF 539 S++ SDC H+ EG C GS ++ G + LP F Sbjct: 585 SASSENSDCPHK-EGECVGSFCNINAKGSSLNNIPKLPRF 623 >SPBC337.13c |gtr1||Gtr1/RagA G protein Gtr1|Schizosaccharomyces pombe|chr 2|||Manual Length = 308 Score = 26.6 bits (56), Expect = 3.4 Identities = 17/46 (36%), Positives = 26/46 (56%), Gaps = 2/46 (4%) Frame = +3 Query: 261 IAEYVQGRNVLCNFHGMDLTTDKLRWMV--KKWQTLIEANIDVKTT 392 +A Q R V C H MDL + LR +V ++ L+E + D++TT Sbjct: 109 VANSPQAR-VFCLIHKMDLVQEDLRDLVFEERKAILLETSKDLETT 153 >SPBC21C3.15c |||aldehyde dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 522 Score = 26.6 bits (56), Expect = 3.4 Identities = 11/26 (42%), Positives = 15/26 (57%) Frame = -3 Query: 356 LPFFNHPPELIGCEVHAVEVAEHITS 279 L H PEL+ C +VA+H+TS Sbjct: 181 LQSMGHSPELVQCITCLPDVADHLTS 206 >SPCC24B10.11c |||THO complex subunit 7 |Schizosaccharomyces pombe|chr 3|||Manual Length = 202 Score = 26.6 bits (56), Expect = 3.4 Identities = 10/22 (45%), Positives = 17/22 (77%) Frame = +3 Query: 621 ARCLQSESESVEEAPFRDLEVD 686 +RCL +++V+EA RDLE++ Sbjct: 22 SRCLGFAAQNVDEANLRDLEIE 43 >SPAC6F12.11c |sfc1||transcription factor TFIIIC complex A box associated subunit Sfc1|Schizosaccharomyces pombe|chr 1|||Manual Length = 456 Score = 26.2 bits (55), Expect = 4.5 Identities = 15/32 (46%), Positives = 17/32 (53%) Frame = -1 Query: 301 KLQSTLRPCTYSAINLICGKTFPRQCQLVDQP 206 KL STLR Y+AIN P Q + VD P Sbjct: 117 KLDSTLRVLDYNAINKFSIDLTPVQRKHVDMP 148 >SPBC1921.05 |ape2||aminopeptidase Ape2|Schizosaccharomyces pombe|chr 2|||Manual Length = 882 Score = 25.4 bits (53), Expect = 7.9 Identities = 12/46 (26%), Positives = 21/46 (45%) Frame = +3 Query: 333 RWMVKKWQTLIEANIDVKTTDGYVLRVFCIGFTNKDSLSQRKTCYA 470 ++ W L+ T GYV+R GFT+ ++ + K +A Sbjct: 795 KFATSNWDKLLSRLPVAGTMRGYVVRFVTSGFTHASAIDKIKEFFA 840 >SPAC664.08c |||traub transciption factor family protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 452 Score = 25.4 bits (53), Expect = 7.9 Identities = 13/34 (38%), Positives = 18/34 (52%), Gaps = 1/34 (2%) Frame = +3 Query: 312 DLTTD-KLRWMVKKWQTLIEANIDVKTTDGYVLR 410 D T D +RWM K Q + N+D K + G +R Sbjct: 351 DSTRDGTVRWMATKKQKQKKENVDTKASKGRKIR 384 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,931,721 Number of Sequences: 5004 Number of extensions: 61869 Number of successful extensions: 174 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 163 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 174 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 325165428 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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