BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS00008 (801 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g32040.1 68415.m03914 integral membrane transporter family pr... 31 1.2 At5g35560.1 68418.m04231 DENN (AEX-3) domain-containing protein ... 29 2.7 At1g79480.1 68414.m09263 hypothetical protein low similarity to ... 29 4.7 At1g73680.1 68414.m08532 pathogen-responsive alpha-dioxygenase, ... 28 6.3 >At2g32040.1 68415.m03914 integral membrane transporter family protein contains 9 transmembrane domains; contains Pfam PF03092: BT1 family; contains TIGRFAMS TIGR00788: folate/biopterin transporter; similar to high affinity folic acid/methotrexate transporter 5 (GI:21898554) [Leishmania tarentolae] Length = 560 Score = 30.7 bits (66), Expect = 1.2 Identities = 13/31 (41%), Positives = 19/31 (61%) Frame = +1 Query: 64 VEGFHCHGWIMKP*YSGLSATVPLFPHTRRS 156 + G W++KP Y +S +VPLF + RRS Sbjct: 165 ITGLSSLPWLVKPLYGFISDSVPLFGYRRRS 195 >At5g35560.1 68418.m04231 DENN (AEX-3) domain-containing protein contains Pfam domain PF02141: DENN (AEX-3) domain; this cDNA may contain an anomalously spliced intron...will require further examination. Length = 765 Score = 29.5 bits (63), Expect = 2.7 Identities = 18/61 (29%), Positives = 24/61 (39%) Frame = -1 Query: 534 LEPIDIHNVMRHPP*DTSSKVSCIATVTVITNKHFNFSHIT*TQHSGCTSLCYRILETRP 355 L P D+ V R D S+ C V I NK + C+SL ++ TR Sbjct: 212 LRPSDLSFVFRLQVADNSTLYGCCLLVEEIVNKPSRLLSTVLDKQPACSSLSRYVMTTRR 271 Query: 354 C 352 C Sbjct: 272 C 272 >At1g79480.1 68414.m09263 hypothetical protein low similarity to beta-1,3-glucanase-like protein GI:9758115 from [Arabidopsis thaliana] Length = 356 Score = 28.7 bits (61), Expect = 4.7 Identities = 13/27 (48%), Positives = 17/27 (62%) Frame = -1 Query: 228 SAVPH*ESGVPAGPEAGRERGPVRGPP 148 S+ P+ VP+ PE+G GPV GPP Sbjct: 197 SSSPNPPEIVPSPPESGYTPGPVLGPP 223 >At1g73680.1 68414.m08532 pathogen-responsive alpha-dioxygenase, putative similar to pathogen-inducible alpha-dioxygenase [Nicotiana attenuata] GI:12539609; contains Pfam profile PF03098: Animal haem peroxidase Length = 631 Score = 28.3 bits (60), Expect = 6.3 Identities = 16/69 (23%), Positives = 33/69 (47%), Gaps = 1/69 (1%) Frame = -1 Query: 651 SKCIFMIFMIANYKKTIIDFFFIA*MGGRAHSPSGIKWLLEPIDIHNVM-RHPP*DTSSK 475 S+ F IF++ ++ D FF + ++ G++W+ + +V+ RH P T Sbjct: 544 SETAFFIFLLVASRRLEADRFFTTNFNEKTYTKEGLEWVNTTETLKDVIDRHFPRLTDQW 603 Query: 474 VSCIATVTV 448 + C + +V Sbjct: 604 MRCSSAFSV 612 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,386,153 Number of Sequences: 28952 Number of extensions: 345679 Number of successful extensions: 696 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 680 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 696 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1814318400 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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