BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS00007 (402 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g47010.1 68418.m05794 RNA helicase, putative similar to type ... 31 0.22 At5g14540.1 68418.m01704 proline-rich family protein contains pr... 29 1.2 At5g51300.2 68418.m06360 splicing factor-related contains simila... 27 3.5 At5g51300.1 68418.m06359 splicing factor-related contains simila... 27 3.5 At3g09090.1 68416.m01069 defective in exine formation protein (D... 27 4.7 At5g44150.1 68418.m05403 expressed protein 27 6.2 At2g15640.1 68415.m01791 F-box family protein contains Pfam PF00... 27 6.2 At5g47850.1 68418.m05912 protein kinase, putative contains simil... 26 8.2 At5g44520.1 68418.m05454 ribose 5-phosphate isomerase-related lo... 26 8.2 At5g25860.1 68418.m03068 F-box family protein contains F-box dom... 26 8.2 At3g44340.1 68416.m04764 sec23/sec24 transport family protein co... 26 8.2 >At5g47010.1 68418.m05794 RNA helicase, putative similar to type 1 RNA helicase pNORF1 [Homo sapiens] GI:1885356 Length = 1254 Score = 31.5 bits (68), Expect = 0.22 Identities = 19/47 (40%), Positives = 23/47 (48%), Gaps = 6/47 (12%) Frame = -1 Query: 267 VPLFPTQGGPRTSPRSHPASGPAGT------PGSR*GTAVGRPPSLG 145 VPL P GGP + P + P GP G PG+ G GR S+G Sbjct: 1002 VPLSPFPGGPPSQPYAIPTRGPVGAVPHAPQPGNH-GFGAGRGTSVG 1047 >At5g14540.1 68418.m01704 proline-rich family protein contains proline rich extensin domains, INTERPRO:IPR002965 Length = 547 Score = 29.1 bits (62), Expect = 1.2 Identities = 16/47 (34%), Positives = 22/47 (46%), Gaps = 1/47 (2%) Frame = -1 Query: 285 SGLSATVPLFPTQGGPRTSPRSHPASGPAGT-PGSR*GTAVGRPPSL 148 SG + P +P Q P PR P+ P G+ P + A PPS+ Sbjct: 349 SGYNPEEPPYPQQSYPPNPPRQPPSHPPPGSAPSQQYYNAPPTPPSM 395 >At5g51300.2 68418.m06360 splicing factor-related contains similarity to SF1 protein [Drosophila melanogaster] GI:6687400 Length = 804 Score = 27.5 bits (58), Expect = 3.5 Identities = 14/51 (27%), Positives = 19/51 (37%) Frame = +2 Query: 188 PGVPAGPEAGCERGLVRGPPCVGNNGTVADNPEYYGFIIQPWQ*NPPTSQT 340 P P P + + G NN +V+ G + PW NPP T Sbjct: 713 PPTPPAPASSTDHSQNMGNMPWANNPSVSTPDHSQGLVNAPWAPNPPMPPT 763 >At5g51300.1 68418.m06359 splicing factor-related contains similarity to SF1 protein [Drosophila melanogaster] GI:6687400 Length = 804 Score = 27.5 bits (58), Expect = 3.5 Identities = 14/51 (27%), Positives = 19/51 (37%) Frame = +2 Query: 188 PGVPAGPEAGCERGLVRGPPCVGNNGTVADNPEYYGFIIQPWQ*NPPTSQT 340 P P P + + G NN +V+ G + PW NPP T Sbjct: 713 PPTPPAPASSTDHSQNMGNMPWANNPSVSTPDHSQGLVNAPWAPNPPMPPT 763 >At3g09090.1 68416.m01069 defective in exine formation protein (DEX1) identical to defective in exine formation [Arabidopsis thaliana] gi|11138669|gb|AAG31444; contains Pfam domain PF01839: FG-GAP repeat Length = 896 Score = 27.1 bits (57), Expect = 4.7 Identities = 11/37 (29%), Positives = 18/37 (48%) Frame = +3 Query: 21 FKILNTEHEMYLKLDVNVDSYGDRKTWGSNDSSEKRH 131 F++L E+ + + D Y D K WG + E +H Sbjct: 373 FELLRENDELADEYSYDYDDYVDEKMWGDEEWVEGQH 409 >At5g44150.1 68418.m05403 expressed protein Length = 355 Score = 26.6 bits (56), Expect = 6.2 Identities = 17/57 (29%), Positives = 24/57 (42%) Frame = +3 Query: 60 LDVNVDSYGDRKTWGSNDSSEKRHTWYLYPVKVGDQQLFLIENREYRQGLKLDANVD 230 L N D Y D + DSS H+ + P G L LI + K++ N+D Sbjct: 57 LPSNWDRYDDELD-AAEDSSISLHSDVIVPKSKGADYLHLISEAQAESNSKIENNLD 112 >At2g15640.1 68415.m01791 F-box family protein contains Pfam PF00646: F-box domain; contains TIGRFAM TIGR01640 : F-box protein interaction domain Length = 426 Score = 26.6 bits (56), Expect = 6.2 Identities = 10/48 (20%), Positives = 27/48 (56%) Frame = +3 Query: 3 ENNNVIFKILNTEHEMYLKLDVNVDSYGDRKTWGSNDSSEKRHTWYLY 146 E++ I+K+ ++ + Y++ ++N D + + W D +++ + Y Y Sbjct: 279 EDDVDIYKLYYSDVDEYVEYNINDDDINELRVWVLEDVKKQQWSKYAY 326 >At5g47850.1 68418.m05912 protein kinase, putative contains similarity to cytokinin-regulated kinase 1 [Nicotiana tabacum] gi|10998537|gb|AAG25966; contains protein kinase domain, Pfam:PF00069 Length = 751 Score = 26.2 bits (55), Expect = 8.2 Identities = 10/23 (43%), Positives = 12/23 (52%) Frame = -3 Query: 220 ASSFRPCRYSRFSMRNSCWSPTF 152 A +FR C R S R CW P + Sbjct: 130 AGNFRICGVERVSRRLRCWQPYY 152 >At5g44520.1 68418.m05454 ribose 5-phosphate isomerase-related low similarity to SP|P47968 Ribose 5-phosphate isomerase (EC 5.3.1.6) (Phosphoriboisomerase) {Mus musculus} Length = 296 Score = 26.2 bits (55), Expect = 8.2 Identities = 9/21 (42%), Positives = 16/21 (76%) Frame = +3 Query: 150 VKVGDQQLFLIENREYRQGLK 212 VKV D+ +F+I+ +Y+ GL+ Sbjct: 152 VKVADEAVFMIKEEQYKAGLE 172 >At5g25860.1 68418.m03068 F-box family protein contains F-box domain Pfam:PF00646 Length = 448 Score = 26.2 bits (55), Expect = 8.2 Identities = 11/16 (68%), Positives = 11/16 (68%) Frame = -3 Query: 217 SSFRPCRYSRFSMRNS 170 S F PCRYSR S NS Sbjct: 263 SGFAPCRYSRTSYLNS 278 >At3g44340.1 68416.m04764 sec23/sec24 transport family protein contains Pfam domains PF04811: Sec23/Sec24 trunk domain, PF04815: Sec23/Sec24 helical domain and PF04810: Sec23/Sec24 zinc finger Length = 1096 Score = 26.2 bits (55), Expect = 8.2 Identities = 20/58 (34%), Positives = 25/58 (43%), Gaps = 7/58 (12%) Frame = -1 Query: 297 KP*YSGLSATVPL------FPTQGGPRTSPRSHPASG-PAGTPGSR*GTAVGRPPSLG 145 +P + G +T PL FP GG P S P G P+G P G PP +G Sbjct: 122 QPPFGGRPSTGPLVGGGSSFPQPGG---FPASGPPGGVPSGPPSGARPIGFGSPPPMG 176 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 8,557,966 Number of Sequences: 28952 Number of extensions: 187505 Number of successful extensions: 621 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 604 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 621 length of database: 12,070,560 effective HSP length: 74 effective length of database: 9,928,112 effective search space used: 585758608 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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