Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00006
(727 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AY568009-1|AAS73299.1| 300|Apis mellifera ADP/ATP translocase p... 156 2e-40
AY332626-1|AAQ24500.1| 300|Apis mellifera ADP/ATP translocase p... 156 2e-40
AB204558-1|BAD89803.1| 1143|Apis mellifera nitric oxide synthase... 22 6.8
>AY568009-1|AAS73299.1| 300|Apis mellifera ADP/ATP translocase
protein.
Length = 300
Score = 156 bits (378), Expect = 2e-40
Identities = 70/84 (83%), Positives = 79/84 (94%)
Frame = +2
Query: 257 ISAAVSKTAVAPIERVKLLLQVQHVSKQIAADQRYKGIVDAFVRIPKEQGLLSFWRGNFA 436
++AA+SKT VAPIERVKLLLQVQH+SKQI+ +QRYKG++D FVRIPKEQG LS+WRGN A
Sbjct: 19 VAAAISKTTVAPIERVKLLLQVQHISKQISEEQRYKGMIDCFVRIPKEQGFLSYWRGNLA 78
Query: 437 NVIRYFPTQALNFAFKDKYKQVFL 508
NVIRYFPTQALNFAFKDKYKQVFL
Sbjct: 79 NVIRYFPTQALNFAFKDKYKQVFL 102
Score = 41.1 bits (92), Expect = 1e-05
Identities = 20/31 (64%), Positives = 24/31 (77%), Gaps = 1/31 (3%)
Frame = +3
Query: 603 PLDFARTRLAADVGKEMA-A*ISGLGNCISR 692
PLDFARTRLAADVGK +GLGNC+++
Sbjct: 135 PLDFARTRLAADVGKAGGEREFTGLGNCLTK 165
Score = 39.1 bits (87), Expect = 4e-05
Identities = 18/32 (56%), Positives = 18/32 (56%)
Frame = +1
Query: 511 GVDKKTQFWRYFXXXXXXXXXXXXTSLCFVYP 606
GVDK TQF RYF TSLCFVYP
Sbjct: 104 GVDKNTQFLRYFVGNLASGGAAGATSLCFVYP 135
Score = 35.9 bits (79), Expect = 4e-04
Identities = 16/17 (94%), Positives = 16/17 (94%)
Frame = +3
Query: 204 MSNLADPVAFAKDFLAG 254
MS LADPVAFAKDFLAG
Sbjct: 1 MSGLADPVAFAKDFLAG 17
Score = 28.7 bits (61), Expect = 0.059
Identities = 19/72 (26%), Positives = 37/72 (51%), Gaps = 3/72 (4%)
Frame = +2
Query: 242 LPGWRISAAVSKTAVA---PIERVKLLLQVQHVSKQIAADQRYKGIVDAFVRIPKEQGLL 412
L W I+ V+ A P + V+ + +Q S + ++ YK + + I K +G
Sbjct: 213 LISWGIAQVVTTVAGIVSYPFDTVRRRMMMQ--SGRAKSEILYKSTLHCWATIYKTEGGN 270
Query: 413 SFWRGNFANVIR 448
+F++G F+N++R
Sbjct: 271 AFFKGAFSNILR 282
Score = 22.2 bits (45), Expect = 5.1
Identities = 18/76 (23%), Positives = 32/76 (42%)
Frame = +2
Query: 260 SAAVSKTAVAPIERVKLLLQVQHVSKQIAADQRYKGIVDAFVRIPKEQGLLSFWRGNFAN 439
+ A S V P++ + L V K ++ + G+ + +I K G+ +RG +
Sbjct: 125 AGATSLCFVYPLDFARTRLAAD-VGKA-GGEREFTGLGNCLTKIFKADGITGLYRGFGVS 182
Query: 440 VIRYFPTQALNFAFKD 487
V +A F F D
Sbjct: 183 VQGIIIYRAAYFGFYD 198
>AY332626-1|AAQ24500.1| 300|Apis mellifera ADP/ATP translocase
protein.
Length = 300
Score = 156 bits (378), Expect = 2e-40
Identities = 70/84 (83%), Positives = 79/84 (94%)
Frame = +2
Query: 257 ISAAVSKTAVAPIERVKLLLQVQHVSKQIAADQRYKGIVDAFVRIPKEQGLLSFWRGNFA 436
++AA+SKT VAPIERVKLLLQVQH+SKQI+ +QRYKG++D FVRIPKEQG LS+WRGN A
Sbjct: 19 VAAAISKTTVAPIERVKLLLQVQHISKQISEEQRYKGMIDCFVRIPKEQGFLSYWRGNLA 78
Query: 437 NVIRYFPTQALNFAFKDKYKQVFL 508
NVIRYFPTQALNFAFKDKYKQVFL
Sbjct: 79 NVIRYFPTQALNFAFKDKYKQVFL 102
Score = 41.1 bits (92), Expect = 1e-05
Identities = 20/31 (64%), Positives = 24/31 (77%), Gaps = 1/31 (3%)
Frame = +3
Query: 603 PLDFARTRLAADVGKEMA-A*ISGLGNCISR 692
PLDFARTRLAADVGK +GLGNC+++
Sbjct: 135 PLDFARTRLAADVGKAGGEREFTGLGNCLTK 165
Score = 39.1 bits (87), Expect = 4e-05
Identities = 18/32 (56%), Positives = 18/32 (56%)
Frame = +1
Query: 511 GVDKKTQFWRYFXXXXXXXXXXXXTSLCFVYP 606
GVDK TQF RYF TSLCFVYP
Sbjct: 104 GVDKNTQFLRYFVGNLASGGAAGATSLCFVYP 135
Score = 35.9 bits (79), Expect = 4e-04
Identities = 16/17 (94%), Positives = 16/17 (94%)
Frame = +3
Query: 204 MSNLADPVAFAKDFLAG 254
MS LADPVAFAKDFLAG
Sbjct: 1 MSGLADPVAFAKDFLAG 17
Score = 28.7 bits (61), Expect = 0.059
Identities = 19/72 (26%), Positives = 37/72 (51%), Gaps = 3/72 (4%)
Frame = +2
Query: 242 LPGWRISAAVSKTAVA---PIERVKLLLQVQHVSKQIAADQRYKGIVDAFVRIPKEQGLL 412
L W I+ V+ A P + V+ + +Q S + ++ YK + + I K +G
Sbjct: 213 LISWGIAQVVTTVAGIVSYPFDTVRRRMMMQ--SGRAKSEILYKSTLHCWATIYKTEGGN 270
Query: 413 SFWRGNFANVIR 448
+F++G F+N++R
Sbjct: 271 AFFKGAFSNILR 282
Score = 22.2 bits (45), Expect = 5.1
Identities = 18/76 (23%), Positives = 32/76 (42%)
Frame = +2
Query: 260 SAAVSKTAVAPIERVKLLLQVQHVSKQIAADQRYKGIVDAFVRIPKEQGLLSFWRGNFAN 439
+ A S V P++ + L V K ++ + G+ + +I K G+ +RG +
Sbjct: 125 AGATSLCFVYPLDFARTRLAAD-VGKA-GGEREFTGLGNCLTKIFKADGITGLYRGFGVS 182
Query: 440 VIRYFPTQALNFAFKD 487
V +A F F D
Sbjct: 183 VQGIIIYRAAYFGFYD 198
>AB204558-1|BAD89803.1| 1143|Apis mellifera nitric oxide synthase
protein.
Length = 1143
Score = 21.8 bits (44), Expect = 6.8
Identities = 11/29 (37%), Positives = 15/29 (51%)
Frame = +1
Query: 436 QRHQVLPDPGAQLRLQGQVQAGVPRGVDK 522
+R Q DP + LQ Q Q+ P G+ K
Sbjct: 773 KRVQTPFDPDVPIELQIQKQSHTPNGIVK 801
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 197,577
Number of Sequences: 438
Number of extensions: 4004
Number of successful extensions: 17
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 17
length of database: 146,343
effective HSP length: 56
effective length of database: 121,815
effective search space used: 22535775
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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