Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00004
(579 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein Ubi3|Schi... 167 1e-42
SPAC589.10c |||ribomal-ubiquitin fusion protein Ubi5|Schizosacch... 167 1e-42
SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein Ubi2|... 111 7e-26
SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein Ubi1|Schiz... 111 7e-26
SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||M... 111 7e-26
SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like pro... 73 2e-14
SPAC6G9.08 |ubp6||ubiquitin C-terminal hydrolase Ubp6|Schizosacc... 38 8e-04
SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyc... 31 0.16
SPAC29B12.06c |rcd1||RNA-binding protein Rcd1 |Schizosaccharomyc... 27 2.6
SPCC1020.08 |||wybutosine biosynthesis protein Tyw1|Schizosaccha... 25 6.1
SPCC1739.03 |hrr1||Helicase Required for RNAi-mediated heterochr... 25 6.1
>SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein
Ubi3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 150
Score = 167 bits (406), Expect = 1e-42
Identities = 86/150 (57%), Positives = 106/150 (70%), Gaps = 2/150 (1%)
Frame = +2
Query: 8 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTL--YG 181
MQIFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTL Y
Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60
Query: 182 LQYPEGIHPPPGVEASWWR*ETQEENYSTPXXXXXXXXXXXLAVLRFYKVDENGKIHRLR 361
+Q +H + + +++ Y+TP LAVL++YKV+++G + RLR
Sbjct: 61 IQKESTLHLVLRLRGGGKK--RKKKTYTTPKKIKHKHKKVELAVLKYYKVEDDGSVKRLR 118
Query: 362 RECTGEQCGAGVFMAVMEDRHYCGKCHSTM 451
REC CGA FMA +DR YCG+CH T+
Sbjct: 119 REC--PNCGASTFMANHKDRLYCGRCHLTL 146
>SPAC589.10c |||ribomal-ubiquitin fusion protein
Ubi5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 150
Score = 167 bits (406), Expect = 1e-42
Identities = 86/150 (57%), Positives = 106/150 (70%), Gaps = 2/150 (1%)
Frame = +2
Query: 8 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTL--YG 181
MQIFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTL Y
Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60
Query: 182 LQYPEGIHPPPGVEASWWR*ETQEENYSTPXXXXXXXXXXXLAVLRFYKVDENGKIHRLR 361
+Q +H + + +++ Y+TP LAVL++YKV+++G + RLR
Sbjct: 61 IQKESTLHLVLRLRGGGKK--RKKKTYTTPKKIKHKHKKVELAVLKYYKVEDDGSVKRLR 118
Query: 362 RECTGEQCGAGVFMAVMEDRHYCGKCHSTM 451
REC CGA FMA +DR YCG+CH T+
Sbjct: 119 REC--PNCGASTFMANHKDRLYCGRCHLTL 146
>SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein
Ubi2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 128
Score = 111 bits (267), Expect = 7e-26
Identities = 55/68 (80%), Positives = 60/68 (88%), Gaps = 2/68 (2%)
Frame = +2
Query: 8 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTL--YG 181
MQIFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTL Y
Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60
Query: 182 LQYPEGIH 205
+Q +H
Sbjct: 61 IQKESTLH 68
Score = 31.5 bits (68), Expect = 0.092
Identities = 13/14 (92%), Positives = 14/14 (100%)
Frame = +3
Query: 177 TDYNIQKESTLHLV 218
+DYNIQKESTLHLV
Sbjct: 57 SDYNIQKESTLHLV 70
>SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein
Ubi1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 128
Score = 111 bits (267), Expect = 7e-26
Identities = 55/68 (80%), Positives = 60/68 (88%), Gaps = 2/68 (2%)
Frame = +2
Query: 8 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTL--YG 181
MQIFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTL Y
Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60
Query: 182 LQYPEGIH 205
+Q +H
Sbjct: 61 IQKESTLH 68
Score = 31.5 bits (68), Expect = 0.092
Identities = 13/14 (92%), Positives = 14/14 (100%)
Frame = +3
Query: 177 TDYNIQKESTLHLV 218
+DYNIQKESTLHLV
Sbjct: 57 SDYNIQKESTLHLV 70
>SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 382
Score = 111 bits (267), Expect = 7e-26
Identities = 55/68 (80%), Positives = 60/68 (88%), Gaps = 2/68 (2%)
Frame = +2
Query: 8 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTL--YG 181
MQIFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTL Y
Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60
Query: 182 LQYPEGIH 205
+Q +H
Sbjct: 61 IQKESTLH 68
Score = 111 bits (267), Expect = 7e-26
Identities = 55/68 (80%), Positives = 60/68 (88%), Gaps = 2/68 (2%)
Frame = +2
Query: 8 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTL--YG 181
MQIFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTL Y
Sbjct: 77 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 136
Query: 182 LQYPEGIH 205
+Q +H
Sbjct: 137 IQKESTLH 144
Score = 111 bits (267), Expect = 7e-26
Identities = 55/68 (80%), Positives = 60/68 (88%), Gaps = 2/68 (2%)
Frame = +2
Query: 8 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTL--YG 181
MQIFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTL Y
Sbjct: 153 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 212
Query: 182 LQYPEGIH 205
+Q +H
Sbjct: 213 IQKESTLH 220
Score = 111 bits (267), Expect = 7e-26
Identities = 55/68 (80%), Positives = 60/68 (88%), Gaps = 2/68 (2%)
Frame = +2
Query: 8 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTL--YG 181
MQIFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTL Y
Sbjct: 229 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 288
Query: 182 LQYPEGIH 205
+Q +H
Sbjct: 289 IQKESTLH 296
Score = 111 bits (267), Expect = 7e-26
Identities = 55/68 (80%), Positives = 60/68 (88%), Gaps = 2/68 (2%)
Frame = +2
Query: 8 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTL--YG 181
MQIFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTL Y
Sbjct: 305 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 364
Query: 182 LQYPEGIH 205
+Q +H
Sbjct: 365 IQKESTLH 372
Score = 31.5 bits (68), Expect = 0.092
Identities = 13/14 (92%), Positives = 14/14 (100%)
Frame = +3
Query: 177 TDYNIQKESTLHLV 218
+DYNIQKESTLHLV
Sbjct: 57 SDYNIQKESTLHLV 70
Score = 31.5 bits (68), Expect = 0.092
Identities = 13/14 (92%), Positives = 14/14 (100%)
Frame = +3
Query: 177 TDYNIQKESTLHLV 218
+DYNIQKESTLHLV
Sbjct: 133 SDYNIQKESTLHLV 146
Score = 31.5 bits (68), Expect = 0.092
Identities = 13/14 (92%), Positives = 14/14 (100%)
Frame = +3
Query: 177 TDYNIQKESTLHLV 218
+DYNIQKESTLHLV
Sbjct: 209 SDYNIQKESTLHLV 222
Score = 31.5 bits (68), Expect = 0.092
Identities = 13/14 (92%), Positives = 14/14 (100%)
Frame = +3
Query: 177 TDYNIQKESTLHLV 218
+DYNIQKESTLHLV
Sbjct: 285 SDYNIQKESTLHLV 298
Score = 31.5 bits (68), Expect = 0.092
Identities = 13/14 (92%), Positives = 14/14 (100%)
Frame = +3
Query: 177 TDYNIQKESTLHLV 218
+DYNIQKESTLHLV
Sbjct: 361 SDYNIQKESTLHLV 374
>SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like
protein modifier Ned8|Schizosaccharomyces pombe|chr
2|||Manual
Length = 78
Score = 73.3 bits (172), Expect = 2e-14
Identities = 30/54 (55%), Positives = 44/54 (81%)
Frame = +2
Query: 8 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGR 169
M I VKTLTGK I L+++P+D + +K ++++KEGIPP+QQRLI+AGKQ+ D +
Sbjct: 1 MLIKVKTLTGKEIELDIDPNDKVSRIKERVEEKEGIPPSQQRLIYAGKQMADDK 54
>SPAC6G9.08 |ubp6||ubiquitin C-terminal hydrolase
Ubp6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 467
Score = 38.3 bits (85), Expect = 8e-04
Identities = 16/43 (37%), Positives = 27/43 (62%)
Frame = +2
Query: 35 GKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLED 163
GK LE+EP++T +K ++ +PP +Q++I G QL+D
Sbjct: 10 GKKYDLEIEPNETGSTLKHQLYSLTQVPPERQKVIVKGGQLKD 52
>SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 354
Score = 30.7 bits (66), Expect = 0.16
Identities = 18/68 (26%), Positives = 33/68 (48%), Gaps = 2/68 (2%)
Frame = +2
Query: 8 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTL--YG 181
+ + +K + + V+ ++ +K I I +QRLI+AG+ L+D +L Y
Sbjct: 4 ISLTIKAANDQKYAVTVDSESSVLALKEAIAPVADIEKERQRLIYAGRVLKDEESLKTYK 63
Query: 182 LQYPEGIH 205
+Q IH
Sbjct: 64 IQDGHSIH 71
>SPAC29B12.06c |rcd1||RNA-binding protein Rcd1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 283
Score = 26.6 bits (56), Expect = 2.6
Identities = 14/54 (25%), Positives = 24/54 (44%), Gaps = 1/54 (1%)
Frame = +2
Query: 311 VLRFY-KVDENGKIHRLRRECTGEQCGAGVFMAVMEDRHYCGKCHSTMVFKDDD 469
V+R Y ++ +N + R C E F V++D H KC + ++ D
Sbjct: 224 VIRCYLRLSDNPRAREALRHCLPEPLRDATFAQVLKDDHNTKKCLAQLLINLSD 277
>SPCC1020.08 |||wybutosine biosynthesis protein
Tyw1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 688
Score = 25.4 bits (53), Expect = 6.1
Identities = 9/30 (30%), Positives = 21/30 (70%), Gaps = 1/30 (3%)
Frame = -2
Query: 185 VVRRGYVHLPAA-FQQRSTSVDWEGFPLCL 99
V +RG + P++ ++++ ++DW+ PLC+
Sbjct: 64 VGKRGKIGSPSSSIRKQNDTIDWKNSPLCV 93
>SPCC1739.03 |hrr1||Helicase Required for RNAi-mediated
heterochromatin assembly Hrr1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1015
Score = 25.4 bits (53), Expect = 6.1
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = -3
Query: 442 VTLPAVVTVLHHRHEHSGAALLAC 371
VTL A+ T+L + H H L+AC
Sbjct: 402 VTLKAIETLLENTHSHVLPILVAC 425
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,288,671
Number of Sequences: 5004
Number of extensions: 44691
Number of successful extensions: 133
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 114
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 127
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 248115846
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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