BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS00003 (711 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC664.05 |rpl13||60S ribosomal protein L13|Schizosaccharomyces... 94 2e-20 SPBC1289.15 ||SPBC8E4.07c|glycoprotein |Schizosaccharomyces pomb... 28 1.1 SPCC622.10c |||exocyst complex subunit Sec5 |Schizosaccharomyces... 27 2.6 SPAC25G10.07c |cut7||kinesin-like protein Cut7|Schizosaccharomyc... 27 3.5 SPAC6F12.10c |ade3|min11|phosphoribosylformylglycinamidine synth... 27 3.5 >SPAC664.05 |rpl13||60S ribosomal protein L13|Schizosaccharomyces pombe|chr 1|||Manual Length = 208 Score = 94.3 bits (224), Expect = 2e-20 Identities = 41/74 (55%), Positives = 51/74 (68%) Frame = +2 Query: 35 IPNGHFHKDWQRFVKTWFNQPARRYRRKQNRIXXXXXXXXXXXXXXLRPIVRCPTVRYHT 214 +PN HFHKDWQR+VKTWFNQP R+ RR+Q R +RP V+ PT+RY+ Sbjct: 9 LPNAHFHKDWQRYVKTWFNQPGRKLRRRQAR-QTKAAKIAPRPVEAIRPAVKPPTIRYNM 67 Query: 215 KVRAGRGFTLREIR 256 KVRAGRGFTL E++ Sbjct: 68 KVRAGRGFTLEELK 81 Score = 59.7 bits (138), Expect = 4e-10 Identities = 37/78 (47%), Positives = 49/78 (62%), Gaps = 1/78 (1%) Frame = +3 Query: 279 ARTIGIAVDPRRRNKSVESLQINVQRIKEYRARLILFP-KGKKVLKGEANEEERKLATQL 455 A TIGI VD RRRN+S ESLQ NV+RIK Y A LI+FP K + KG+A + T + Sbjct: 90 ASTIGIPVDHRRRNRSEESLQRNVERIKVYLAHLIVFPRKAGQPKKGDATDVSGAEQTDV 149 Query: 456 RGPLMPVQQPAPKSVARP 509 ++P+ Q A + A+P Sbjct: 150 -AAVLPITQEAVEE-AKP 165 Score = 33.9 bits (74), Expect = 0.023 Identities = 17/41 (41%), Positives = 22/41 (53%) Frame = +2 Query: 503 KTITEDEKNFKAYQYLRGARSIAKLVGIRAKRLKDAAENPD 625 K ITE+ KNF A+ L R+ A+ G RA K AE + Sbjct: 164 KPITEEAKNFNAFSTLSNERAYARYAGARAAFQKKRAEEAE 204 >SPBC1289.15 ||SPBC8E4.07c|glycoprotein |Schizosaccharomyces pombe|chr 2|||Manual Length = 1283 Score = 28.3 bits (60), Expect = 1.1 Identities = 16/44 (36%), Positives = 20/44 (45%), Gaps = 4/44 (9%) Frame = -3 Query: 247 TKSESSTGAYFSM----VPNSWASHYRT*RPSCRTWSYGLSFLY 128 T +STG+Y M + W S T C TWSY S+ Y Sbjct: 1215 TVQGTSTGSYICMPHFQIQYDWCSAGVTDMSECNTWSYQKSYDY 1258 >SPCC622.10c |||exocyst complex subunit Sec5 |Schizosaccharomyces pombe|chr 3|||Manual Length = 815 Score = 27.1 bits (57), Expect = 2.6 Identities = 12/39 (30%), Positives = 20/39 (51%) Frame = -1 Query: 303 LQQFQSFGQILGSICGLISRRVNPLPARTLVWYRTVGHR 187 LQ F ++ ++C L+ + NP+ R V Y TV + Sbjct: 292 LQSQGDFAFVVRTLCDLLKLKPNPISGRDPVLYATVSQQ 330 >SPAC25G10.07c |cut7||kinesin-like protein Cut7|Schizosaccharomyces pombe|chr 1|||Manual Length = 1085 Score = 26.6 bits (56), Expect = 3.5 Identities = 11/31 (35%), Positives = 20/31 (64%) Frame = +2 Query: 527 NFKAYQYLRGARSIAKLVGIRAKRLKDAAEN 619 N ++ ++LR A S A++VG +R++ EN Sbjct: 843 NNRSEEFLRNAASQAEIVGANKERIQKTVEN 873 >SPAC6F12.10c |ade3|min11|phosphoribosylformylglycinamidine synthase Ade3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1323 Score = 26.6 bits (56), Expect = 3.5 Identities = 14/27 (51%), Positives = 16/27 (59%) Frame = +2 Query: 227 GRGFTLREIRPQIEPSICPNDWNCCRS 307 G F LR+I P IEPS+ P CC S Sbjct: 527 GARFELRDI-PCIEPSMSPMQIWCCES 552 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,697,038 Number of Sequences: 5004 Number of extensions: 54172 Number of successful extensions: 160 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 151 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 159 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 331187010 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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