BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00003
(711 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC664.05 |rpl13||60S ribosomal protein L13|Schizosaccharomyces... 94 2e-20
SPBC1289.15 ||SPBC8E4.07c|glycoprotein |Schizosaccharomyces pomb... 28 1.1
SPCC622.10c |||exocyst complex subunit Sec5 |Schizosaccharomyces... 27 2.6
SPAC25G10.07c |cut7||kinesin-like protein Cut7|Schizosaccharomyc... 27 3.5
SPAC6F12.10c |ade3|min11|phosphoribosylformylglycinamidine synth... 27 3.5
>SPAC664.05 |rpl13||60S ribosomal protein L13|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 208
Score = 94.3 bits (224), Expect = 2e-20
Identities = 41/74 (55%), Positives = 51/74 (68%)
Frame = +2
Query: 35 IPNGHFHKDWQRFVKTWFNQPARRYRRKQNRIXXXXXXXXXXXXXXLRPIVRCPTVRYHT 214
+PN HFHKDWQR+VKTWFNQP R+ RR+Q R +RP V+ PT+RY+
Sbjct: 9 LPNAHFHKDWQRYVKTWFNQPGRKLRRRQAR-QTKAAKIAPRPVEAIRPAVKPPTIRYNM 67
Query: 215 KVRAGRGFTLREIR 256
KVRAGRGFTL E++
Sbjct: 68 KVRAGRGFTLEELK 81
Score = 59.7 bits (138), Expect = 4e-10
Identities = 37/78 (47%), Positives = 49/78 (62%), Gaps = 1/78 (1%)
Frame = +3
Query: 279 ARTIGIAVDPRRRNKSVESLQINVQRIKEYRARLILFP-KGKKVLKGEANEEERKLATQL 455
A TIGI VD RRRN+S ESLQ NV+RIK Y A LI+FP K + KG+A + T +
Sbjct: 90 ASTIGIPVDHRRRNRSEESLQRNVERIKVYLAHLIVFPRKAGQPKKGDATDVSGAEQTDV 149
Query: 456 RGPLMPVQQPAPKSVARP 509
++P+ Q A + A+P
Sbjct: 150 -AAVLPITQEAVEE-AKP 165
Score = 33.9 bits (74), Expect = 0.023
Identities = 17/41 (41%), Positives = 22/41 (53%)
Frame = +2
Query: 503 KTITEDEKNFKAYQYLRGARSIAKLVGIRAKRLKDAAENPD 625
K ITE+ KNF A+ L R+ A+ G RA K AE +
Sbjct: 164 KPITEEAKNFNAFSTLSNERAYARYAGARAAFQKKRAEEAE 204
>SPBC1289.15 ||SPBC8E4.07c|glycoprotein |Schizosaccharomyces pombe|chr
2|||Manual
Length = 1283
Score = 28.3 bits (60), Expect = 1.1
Identities = 16/44 (36%), Positives = 20/44 (45%), Gaps = 4/44 (9%)
Frame = -3
Query: 247 TKSESSTGAYFSM----VPNSWASHYRT*RPSCRTWSYGLSFLY 128
T +STG+Y M + W S T C TWSY S+ Y
Sbjct: 1215 TVQGTSTGSYICMPHFQIQYDWCSAGVTDMSECNTWSYQKSYDY 1258
>SPCC622.10c |||exocyst complex subunit Sec5 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 815
Score = 27.1 bits (57), Expect = 2.6
Identities = 12/39 (30%), Positives = 20/39 (51%)
Frame = -1
Query: 303 LQQFQSFGQILGSICGLISRRVNPLPARTLVWYRTVGHR 187
LQ F ++ ++C L+ + NP+ R V Y TV +
Sbjct: 292 LQSQGDFAFVVRTLCDLLKLKPNPISGRDPVLYATVSQQ 330
>SPAC25G10.07c |cut7||kinesin-like protein Cut7|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1085
Score = 26.6 bits (56), Expect = 3.5
Identities = 11/31 (35%), Positives = 20/31 (64%)
Frame = +2
Query: 527 NFKAYQYLRGARSIAKLVGIRAKRLKDAAEN 619
N ++ ++LR A S A++VG +R++ EN
Sbjct: 843 NNRSEEFLRNAASQAEIVGANKERIQKTVEN 873
>SPAC6F12.10c |ade3|min11|phosphoribosylformylglycinamidine synthase
Ade3 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1323
Score = 26.6 bits (56), Expect = 3.5
Identities = 14/27 (51%), Positives = 16/27 (59%)
Frame = +2
Query: 227 GRGFTLREIRPQIEPSICPNDWNCCRS 307
G F LR+I P IEPS+ P CC S
Sbjct: 527 GARFELRDI-PCIEPSMSPMQIWCCES 552
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,697,038
Number of Sequences: 5004
Number of extensions: 54172
Number of successful extensions: 160
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 151
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 159
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 331187010
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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