BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fprWP13_F_J24
(648 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1006.07 |||translation initiation factor eIF4A|Schizosacchar... 28 1.0
SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccha... 27 1.8
SPBC1271.01c |pof13||F-box protein Pof13|Schizosaccharomyces pom... 27 3.1
SPAPB1A10.09 |ase1||microtubule-associated protein Ase1 |Schizos... 25 7.1
SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 25 7.1
SPBC3D6.16 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 25 7.1
SPAC27E2.09 |mak2|phk1|histidine kinase Mak2 |Schizosaccharomyce... 25 7.1
SPAC6B12.09 |trm10||tRNA m|Schizosaccharomyces pombe|chr 1|||Manual 25 7.1
SPAC19A8.05c |vps27|sst4|sorting receptor for ubiquitinated memb... 25 9.4
SPCC4B3.15 |mid1|dmf1|medial ring protein Mid1|Schizosaccharomyc... 25 9.4
SPAC57A10.02 |cdr2||GIN4 family protein kinase Cdr2|Schizosaccha... 25 9.4
>SPAC1006.07 |||translation initiation factor
eIF4A|Schizosaccharomyces pombe|chr 1|||Manual
Length = 392
Score = 28.3 bits (60), Expect = 1.0
Identities = 9/21 (42%), Positives = 17/21 (80%)
Frame = +3
Query: 285 AIDLLTNDECRLLLEVEDFFN 347
+I+ +TND+ R++ E+E F+N
Sbjct: 358 SINFVTNDDVRMMREIEQFYN 378
>SPAC1834.01 |sup45||translation release factor
eRF1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 433
Score = 27.5 bits (58), Expect = 1.8
Identities = 12/30 (40%), Positives = 16/30 (53%)
Frame = +1
Query: 418 STRHHRRS*NQVPPNSLTIFCSS*TVNANK 507
STR + N+VP N L I+C + NK
Sbjct: 74 STRERLKLYNKVPDNGLVIYCGEVIMEGNK 103
>SPBC1271.01c |pof13||F-box protein Pof13|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 396
Score = 26.6 bits (56), Expect = 3.1
Identities = 24/85 (28%), Positives = 36/85 (42%), Gaps = 2/85 (2%)
Frame = +2
Query: 47 FXCNFAITMGF*YLIVCTPACAFTHN-IKMLAKMAPS-QDKLVHLHKTPTSLDINPSGLL 220
F C I++ F I A A H I L + P +D H K I+P LL
Sbjct: 310 FVCPSCISLDFDLQI---QAFARQHRVISTLGVVYPEREDSCFHKIKAAQWHQISPRSLL 366
Query: 221 FAFVELNHYNNECESEGVMGCGHRF 295
F F E NH +++ ++ G ++
Sbjct: 367 FQFQEQNHIHHKKIRRKLLAAGWKW 391
>SPAPB1A10.09 |ase1||microtubule-associated protein Ase1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 731
Score = 25.4 bits (53), Expect = 7.1
Identities = 13/40 (32%), Positives = 22/40 (55%), Gaps = 1/40 (2%)
Frame = +3
Query: 486 LDSQCKQENIFSNWLEEKVD-LPSIFENISEVPERVDPQP 602
+D CKQ+ +FS E++ D L SI +S + ++ P
Sbjct: 242 IDQLCKQKEVFSAEKEKRSDHLKSIQSEVSNLWNKLQVSP 281
>SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 583
Score = 25.4 bits (53), Expect = 7.1
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = +3
Query: 303 NDECRLLLEVEDFFNDDC 356
NDECR L + FF+ +C
Sbjct: 535 NDECRRLKQCNHFFHREC 552
>SPBC3D6.16 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 95
Score = 25.4 bits (53), Expect = 7.1
Identities = 12/29 (41%), Positives = 14/29 (48%)
Frame = +2
Query: 158 DKLVHLHKTPTSLDINPSGLLFAFVELNH 244
D + H K +S NPS LLF L H
Sbjct: 59 DGIPHSRKKVSSAHFNPSTLLFLLKRLGH 87
>SPAC27E2.09 |mak2|phk1|histidine kinase Mak2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2310
Score = 25.4 bits (53), Expect = 7.1
Identities = 14/49 (28%), Positives = 23/49 (46%)
Frame = -2
Query: 428 WRVETRSVIDFDLRCSARKVFKQITIIIEEILNFEQQTALIVSEQIDGR 282
W T+ V R S RKV + + + EE +++ ALI + + R
Sbjct: 2143 WSTPTKLVEPSQFRASPRKVDQAVVLSSEEKEILQKKYALIAEDNLIAR 2191
>SPAC6B12.09 |trm10||tRNA m|Schizosaccharomyces pombe|chr 1|||Manual
Length = 304
Score = 25.4 bits (53), Expect = 7.1
Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 4/49 (8%)
Frame = +2
Query: 134 LAKMAPSQDKLVHLH----KTPTSLDINPSGLLFAFVELNHYNNECESE 268
L + ++KLV+L T T LD + ++ A V+ N Y N C+++
Sbjct: 170 LEEFESQKEKLVYLSADSDNTITELDEDKIYIIGAIVDKNRYKNLCQNK 218
>SPAC19A8.05c |vps27|sst4|sorting receptor for ubiquitinated
membrane proteins, ESCRT 0 complex|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 610
Score = 25.0 bits (52), Expect = 9.4
Identities = 14/40 (35%), Positives = 20/40 (50%)
Frame = +3
Query: 528 LEEKVDLPSIFENISEVPERVDPQPPAAVLASSPFVTSQP 647
+E + D+ S N S +P+ P V+ SSP VT P
Sbjct: 470 IEPEPDVISTVRNSSTIPQASSSSVPKIVVDSSP-VTENP 508
>SPCC4B3.15 |mid1|dmf1|medial ring protein Mid1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 920
Score = 25.0 bits (52), Expect = 9.4
Identities = 10/21 (47%), Positives = 14/21 (66%)
Frame = +3
Query: 564 NISEVPERVDPQPPAAVLASS 626
N++ +P V P PPA + ASS
Sbjct: 221 NLARIPSDVKPVPPAHLSASS 241
>SPAC57A10.02 |cdr2||GIN4 family protein kinase
Cdr2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 775
Score = 25.0 bits (52), Expect = 9.4
Identities = 15/55 (27%), Positives = 25/55 (45%)
Frame = +1
Query: 445 NQVPPNSLTIFCSS*TVNANKRIFSPTG*RKKLTSLRSSKTFLKFLNAWTHNPRQ 609
N + N+ ++ T+N ++R+ +P+G R L S LN H RQ
Sbjct: 549 NNIDNNNYNQPYANATMNNSRRLRTPSGERSMRADLSQSPASYDSLNVPKHRRRQ 603
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,496,292
Number of Sequences: 5004
Number of extensions: 47947
Number of successful extensions: 127
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 125
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 127
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 291768710
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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