Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fprWP13_F_F05
(631 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 92 5e-20
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 90 2e-19
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 81 1e-16
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 53 3e-08
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 53 3e-08
SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 29 0.55
SPBC4F6.07c |||ATP-dependent RNA helicase Mak5 |Schizosaccharomy... 28 1.3
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 27 1.7
SPAC1565.07c |||TATA binding protein interacting protein |Schizo... 26 3.9
SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|... 26 3.9
SPAC3H1.12c |snt2||Lid2 complex subunit Snt2 |Schizosaccharomyce... 25 9.0
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 92.3 bits (219), Expect = 5e-20
Identities = 49/131 (37%), Positives = 80/131 (61%), Gaps = 2/131 (1%)
Frame = +2
Query: 206 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDER--IS 379
+++Q+AEF+EAF LFD DG I ++G +R+LGQ+PT ++++ + D I
Sbjct: 7 TDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTID 66
Query: 380 FEVFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVX 559
F FL + K + D + E + FDKDGNG+I+ EL H+L++LGE+LS +EV
Sbjct: 67 FTEFLTMMAR--KMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVA 124
Query: 560 QLLQGQEDSQG 592
+++ + D+ G
Sbjct: 125 DMIR-EADTDG 134
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 90.2 bits (214), Expect = 2e-19
Identities = 47/116 (40%), Positives = 71/116 (61%)
Frame = +2
Query: 227 FQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFEVFLPIYQ 406
+++AF LFD G G+I IGD LRA GQNPT +++ + L P E + E FL +
Sbjct: 8 YKQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEITEIESTL-PAE-VDMEQFLQVLN 65
Query: 407 AISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVXQLLQG 574
+ +F++G + FDKD G I ELR++L++LGEKLS++E+ +LL+G
Sbjct: 66 RPNGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKG 121
Score = 27.5 bits (58), Expect = 1.7
Identities = 12/38 (31%), Positives = 21/38 (55%)
Frame = +2
Query: 224 EFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDV 337
EF + FQ+FD G I V ++ L +LG+ + ++
Sbjct: 78 EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEM 115
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 81.4 bits (192), Expect = 1e-16
Identities = 44/122 (36%), Positives = 73/122 (59%)
Frame = +2
Query: 206 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFE 385
S++Q E +EAF L+D DG I + +G LR+LG N T++++ K + L + I +
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNEL--GDAIDEK 61
Query: 386 VFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVXQL 565
F+ +K R ++ ++I+ R FDKD +G+I +A+ + TLGEKLSD+EV +
Sbjct: 62 KFMSFVS--NKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLM 119
Query: 566 LQ 571
+Q
Sbjct: 120 VQ 121
Score = 35.5 bits (78), Expect = 0.006
Identities = 16/65 (24%), Positives = 35/65 (53%)
Frame = +2
Query: 224 EFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFEVFLPIY 403
E+ +AF++FD G I A+ D ++ LG+ ++++V+ P SF+ + +
Sbjct: 79 EYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEADPTNSGSFDYYDFVQ 138
Query: 404 QAISK 418
+ ++K
Sbjct: 139 RIMAK 143
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 53.2 bits (122), Expect = 3e-08
Identities = 35/125 (28%), Positives = 58/125 (46%)
Frame = +2
Query: 197 AGYSEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERI 376
A + Q+ E +EAF L D GDG I + L +L Q+ +E + + P I
Sbjct: 40 AQLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHMFESINPP--I 97
Query: 377 SFEVFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEV 556
+ FL ++ S ND +E FD +G I + +R LS++G+++ EV
Sbjct: 98 NLAAFLTAMGSMLCRIS--PRNDLLEAFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEV 155
Query: 557 XQLLQ 571
+L+
Sbjct: 156 ESILR 160
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 53.2 bits (122), Expect = 3e-08
Identities = 36/124 (29%), Positives = 56/124 (45%), Gaps = 2/124 (1%)
Frame = +2
Query: 206 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDER--IS 379
+E+Q + EAF+LFDS D I ++ A+RALG N +S+V K + +
Sbjct: 32 TEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQ 91
Query: 380 FEVFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVX 559
E F+ + K D + FD D G IS LR + L E + D E+
Sbjct: 92 MEDFVRV--MTEKIVERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELE 149
Query: 560 QLLQ 571
+++
Sbjct: 150 AMIE 153
>SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3699
Score = 29.1 bits (62), Expect = 0.55
Identities = 19/57 (33%), Positives = 29/57 (50%)
Frame = -1
Query: 559 LYLIITELLSESREQVSQFRRRDEPIAIFVKMAQTLNKVISSVATACFRYGLVNWQK 389
L+L+ L++ +Q + R + IAIF K + +K I SVA R+ L QK
Sbjct: 1377 LHLLFASLVAHKFDQPQHAQTRTKIIAIFFKDLYSPHKEIYSVAIDALRHVLSQNQK 1433
>SPBC4F6.07c |||ATP-dependent RNA helicase Mak5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 648
Score = 27.9 bits (59), Expect = 1.3
Identities = 18/49 (36%), Positives = 24/49 (48%)
Frame = +2
Query: 473 KDGNGFISSAELRHLLSTLGEKLSDDEVXQLLQGQEDSQGKYQL*XLGS 619
KDG A L+ + LG + SDDE L + E S+ K Q+ L S
Sbjct: 544 KDGRVGREEAWLKSMAQELGVESSDDEDPDLPKKSESSKNKKQIAHLRS 592
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 27.5 bits (58), Expect = 1.7
Identities = 18/64 (28%), Positives = 30/64 (46%)
Frame = +2
Query: 224 EFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFEVFLPIY 403
E +EAF LFD G I + + LG+N T+ ++ + ++S E F ++
Sbjct: 13 EAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLDLAGTNGKVSREEFAELW 72
Query: 404 QAIS 415
IS
Sbjct: 73 IHIS 76
Score = 25.8 bits (54), Expect = 5.2
Identities = 14/43 (32%), Positives = 23/43 (53%)
Frame = +2
Query: 467 FDKDGNGFISSAELRHLLSTLGEKLSDDEVXQLLQGQEDSQGK 595
FD G+I +LR + LGE L+ +++ QL+ + GK
Sbjct: 21 FDVTHKGYIDFEDLRRSCAQLGENLTKEQL-QLMLDLAGTNGK 62
>SPAC1565.07c |||TATA binding protein interacting protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1220
Score = 26.2 bits (55), Expect = 3.9
Identities = 18/67 (26%), Positives = 35/67 (52%), Gaps = 3/67 (4%)
Frame = +2
Query: 383 EVFLPIYQAISKARSGDTANDFI--EGLRHFDKDGNGFISS-AELRHLLSTLGEKLSDDE 553
++F+ + I+K +G + I + RH DK GN F ++ EL +L +G+K +
Sbjct: 643 DIFMSVTD-ITKIENGTKIYEEILQDCCRHIDKSGNEFTTAYLELLEVLLKVGQKYLAES 701
Query: 554 VXQLLQG 574
+ + + G
Sbjct: 702 LLEHILG 708
>SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1752
Score = 26.2 bits (55), Expect = 3.9
Identities = 16/64 (25%), Positives = 29/64 (45%), Gaps = 2/64 (3%)
Frame = -3
Query: 305 LLTHLRSERHVSFHLHEN--RRAGRLLEILPIDPLNIQPYYESTNEIEGNEIGIFKGCDL 132
+ TH+ SE L+E R ++ +LP+ P +++P G + K D+
Sbjct: 216 IFTHISSEDLAHLGLNEQYARPDWMIITVLPVPPPSVRPSISVDGTSRGEDDLTHKLSDI 275
Query: 131 SKSN 120
K+N
Sbjct: 276 IKAN 279
>SPAC3H1.12c |snt2||Lid2 complex subunit Snt2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1131
Score = 25.0 bits (52), Expect = 9.0
Identities = 8/14 (57%), Positives = 10/14 (71%)
Frame = +1
Query: 484 WVHLFCGTATPALY 525
WVHL C + TP +Y
Sbjct: 927 WVHLICASWTPDVY 940
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,401,415
Number of Sequences: 5004
Number of extensions: 47140
Number of successful extensions: 183
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 164
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 172
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 279695522
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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