Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fprWP12_F_M17
         (490 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   213   2e-56
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   122   3e-29
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   117   1e-27
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...   113   1e-26
SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyc...    27   1.1  
SPBC19G7.16 |iws1||transcription elongation factor complex subun...    27   1.5  
SPAC12G12.06c |||RNA 3'-terminal phosphate cyclase |Schizosaccha...    26   2.7  
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos...    26   2.7  
SPCC18.03 |||shuttle craft like transcriptional regulator|Schizo...    26   3.5  
SPAC8C9.04 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||...    26   3.5  
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz...    25   4.6  
SPAC664.04c |rps1602|rps16-2, rps16|40S ribosomal protein S16|Sc...    25   6.1  
SPBC18H10.14 |rps1601|rps16-1|40S ribosomal protein S16|Schizosa...    25   6.1  

>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  213 bits (519), Expect = 2e-56
 Identities = 94/135 (69%), Positives = 110/135 (81%), Gaps = 1/135 (0%)
 Frame = +2

Query: 53  MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 232
           MREIVH+QAGQCGNQ+GA FW  I+DEHG+D  G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60

Query: 233 PRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTXGAXLVDSVLDVV 412
           PRA+LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYT GA L D+VLDVV
Sbjct: 61  PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120

Query: 413 RKNXNLAIA-TGLQL 454
           R+      A  G QL
Sbjct: 121 RREAEACDALQGFQL 135


>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  122 bits (294), Expect = 3e-29
 Identities = 55/124 (44%), Positives = 77/124 (62%), Gaps = 2/124 (1%)
 Frame = +2

Query: 53  MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLER--INVYYNEASGGK 226
           MREI+ +  GQ G QIG   WE+   EHGI P G  + ++  Q      + +++E   GK
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60

Query: 227 YVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTXGAXLVDSVLD 406
           YVPR+I VDLEP  +D VR+GP+  +F P+  + G+  A NN+A+GHYT G  LVD V D
Sbjct: 61  YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120

Query: 407 VVRK 418
            +R+
Sbjct: 121 KIRR 124


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  117 bits (281), Expect = 1e-27
 Identities = 53/128 (41%), Positives = 78/128 (60%), Gaps = 6/128 (4%)
 Frame = +2

Query: 53  MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 214
           MRE++ +  GQ G QIG   WE+   EHGI P G        H ++    +    +++E 
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60

Query: 215 SGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTXGAXLVD 394
             GK+VPR+I VDLEP  +D VR+GP+  +F P+  V G+  A NN+A+GHYT G  ++D
Sbjct: 61  GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120

Query: 395 SVLDVVRK 418
           SVL+ +R+
Sbjct: 121 SVLERIRR 128


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score =  113 bits (272), Expect = 1e-26
 Identities = 48/121 (39%), Positives = 82/121 (67%), Gaps = 2/121 (1%)
 Frame = +2

Query: 56  REIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 235
           REI+ LQAGQCGNQIG++FW+ +  EHGI P G     +   ++R +V++ ++   +Y+P
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62

Query: 236 RAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTXGAXLVDSVLDV 409
           RAIL+DLEP  ++++ S  +G ++ P+N +  ++  GAGNNWA G Y+    + + ++D+
Sbjct: 63  RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDM 121

Query: 410 V 412
           +
Sbjct: 122 I 122


>SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 587

 Score = 27.5 bits (58), Expect = 1.1
 Identities = 15/35 (42%), Positives = 18/35 (51%)
 Frame = +3

Query: 180 SWSASMYTTMKPPAASTCPAPFSSTWSPAPWTLSA 284
           S +AS      PP A+   +  SST SPAPW   A
Sbjct: 396 SGAASPQAGHPPPWAAASTSVSSSTSSPAPWAKPA 430


>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
           Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 428

 Score = 27.1 bits (57), Expect = 1.5
 Identities = 10/34 (29%), Positives = 22/34 (64%)
 Frame = +2

Query: 92  NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 193
           N++G    E+++++  +DPT A   + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166


>SPAC12G12.06c |||RNA 3'-terminal phosphate cyclase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 363

 Score = 26.2 bits (55), Expect = 2.7
 Identities = 11/19 (57%), Positives = 15/19 (78%)
 Frame = +3

Query: 36  KITYTK*GKSFIYRPANVV 92
           +I+YT  G SFIYRP N++
Sbjct: 64  EISYT--GTSFIYRPGNII 80


>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
           Pof11|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 506

 Score = 26.2 bits (55), Expect = 2.7
 Identities = 10/25 (40%), Positives = 15/25 (60%), Gaps = 1/25 (4%)
 Frame = +2

Query: 287 GPFGQIFRPDNFVFGQSGAG-NNWA 358
           GP+G +F P  F+F  +G    NW+
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWS 181


>SPCC18.03 |||shuttle craft like transcriptional
           regulator|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1077

 Score = 25.8 bits (54), Expect = 3.5
 Identities = 17/65 (26%), Positives = 21/65 (32%)
 Frame = -3

Query: 251 RREWRGARTCRRRLHCSIH*CAPTASQSPHGRHRWGRCRARQR*SPRT*LQSGYHIGRPV 72
           R   R    C + L C  H C               +   ++R       QS  H G P 
Sbjct: 693 RENVRCGELCNKLLSCKTHFCEKLCHPDGECESSCKKECGKRRMYCEHVCQSPCHAGHPC 752

Query: 71  DERFP 57
           DER P
Sbjct: 753 DERIP 757


>SPAC8C9.04 |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 647

 Score = 25.8 bits (54), Expect = 3.5
 Identities = 15/44 (34%), Positives = 20/44 (45%), Gaps = 4/44 (9%)
 Frame = +3

Query: 150 PVPTMGTLT----CSWSASMYTTMKPPAASTCPAPFSSTWSPAP 269
           P+PT          + S S  T   P +A+T P P S+T  P P
Sbjct: 544 PIPTSADTVESKHAAGSGSATTIPSPGSATTKPTPGSATTKPTP 587


>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 255

 Score = 25.4 bits (53), Expect = 4.6
 Identities = 13/32 (40%), Positives = 16/32 (50%)
 Frame = -2

Query: 345 PAPDCPKTKLSGRKICPKGPERTESMVPGSKS 250
           P+   PK  L  R I P GPE  +  + GS S
Sbjct: 20  PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51


>SPAC664.04c |rps1602|rps16-2, rps16|40S ribosomal protein
           S16|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 140

 Score = 25.0 bits (52), Expect = 6.1
 Identities = 19/54 (35%), Positives = 29/54 (53%), Gaps = 5/54 (9%)
 Frame = -1

Query: 373 CVVSLGPV-VAGAGLS--EDEVVRTEDLSER--SGADRVHGAGLQVDENGAGHV 227
           C V  G + V GA LS  + E++R +       +GAD+  G  ++V  +G GHV
Sbjct: 19  CKVGKGLIKVNGAPLSLVQPEILRMKVYEPILVAGADKFAGVDIRVRVSGGGHV 72


>SPBC18H10.14 |rps1601|rps16-1|40S ribosomal protein
           S16|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 140

 Score = 25.0 bits (52), Expect = 6.1
 Identities = 19/54 (35%), Positives = 29/54 (53%), Gaps = 5/54 (9%)
 Frame = -1

Query: 373 CVVSLGPV-VAGAGLS--EDEVVRTEDLSER--SGADRVHGAGLQVDENGAGHV 227
           C V  G + V GA LS  + E++R +       +GAD+  G  ++V  +G GHV
Sbjct: 19  CKVGKGLIKVNGAPLSLVQPEILRMKVYEPILVAGADKFAGVDIRVRVSGGGHV 72


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,953,775
Number of Sequences: 5004
Number of extensions: 37817
Number of successful extensions: 118
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 108
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 114
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 190087364
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)