Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fprWP12_F_J19
         (650 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC1687.13c |csn5||COP9/signalosome complex subunit Csn5|Schizo...    47   2e-06
SPAC31G5.13 |rpn11|pad1, sks1, bfr2, mts5|19S proteasome regulat...    42   8e-05
SPCC1682.10 |rpn8||19S proteasome regulatory subunit Rpn8|Schizo...    33   0.036
SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual    29   0.77 
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1...    27   3.1  
SPCC132.01c ||SPCC1322.17c|DUF814 family protein|Schizosaccharom...    25   7.2  
SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gc...    25   9.5  

>SPAC1687.13c |csn5||COP9/signalosome complex subunit
           Csn5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 299

 Score = 47.2 bits (107), Expect = 2e-06
 Identities = 32/103 (31%), Positives = 51/103 (49%)
 Frame = +2

Query: 89  EMLQKVRLSSDVALVCMQHALSTEKEEIMGLLIGEVHDNGALVSIVSSVILRRLDKKPDR 268
           E  + V++S+   L  ++H       E+MG + G+V   GA + I+ S  L  ++    R
Sbjct: 30  EFFRSVKISAVALLKMLRHVSQGMPLEVMGYVQGKVE--GASLIILDSFALP-VEGTETR 86

Query: 269 VEISEEQLVQATVRAEELAAEVGQPLRVVGWYHSHPHITVWPS 397
           V   EE   + +V+   L   V +   V+GWYHSHP+   W S
Sbjct: 87  VNAHEEAQ-EYSVQYHTLCKSVYRHENVIGWYHSHPNYGCWLS 128


>SPAC31G5.13 |rpn11|pad1, sks1, bfr2, mts5|19S proteasome regulatory
           subunit Rpn11|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 308

 Score = 41.9 bits (94), Expect = 8e-05
 Identities = 29/99 (29%), Positives = 47/99 (47%), Gaps = 1/99 (1%)
 Frame = +2

Query: 104 VRLSSDVALVCMQHALSTEKEEIMGLLIGEVHDNGALVSIVSSVILRRLDKKPDRVEISE 283
           V +SS   L  ++H       E+MGL++GE  D+      V  V +  + +    V +  
Sbjct: 30  VYISSLALLKMLRHGRHGTPMEVMGLMLGEFVDDFT----VRVVDVFAMPQSGTGVSVEA 85

Query: 284 -EQLVQATVRAEELAAEVGQPLRVVGWYHSHPHITVWPS 397
            + + Q  +   ++  + G+P  VVGWYHSHP    W S
Sbjct: 86  VDPVFQKNMM--DMLKQTGRPEMVVGWYHSHPGFGCWLS 122


>SPCC1682.10 |rpn8||19S proteasome regulatory subunit
           Rpn8|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 324

 Score = 33.1 bits (72), Expect = 0.036
 Identities = 18/75 (24%), Positives = 41/75 (54%), Gaps = 1/75 (1%)
 Frame = +2

Query: 161 KEEIMGLLIGEVHDNGALVSIVSSVILR-RLDKKPDRVEISEEQLVQATVRAEELAAEVG 337
           K  ++G+L+G+  +NG +V++ +S  +    D+K   V   +   +++     E+  ++ 
Sbjct: 39  KRRVVGILLGQ--NNGDVVNVANSYAIPFEEDEKNASVWFLDHNFMESM---NEMFKKIN 93

Query: 338 QPLRVVGWYHSHPHI 382
              ++VGWYH+ P +
Sbjct: 94  ANEKLVGWYHTGPQL 108


>SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1236

 Score = 28.7 bits (61), Expect = 0.77
 Identities = 28/91 (30%), Positives = 44/91 (48%), Gaps = 1/91 (1%)
 Frame = -3

Query: 357 PTTLRGWPTSAANSSALTVA*TSCSS-DISTRSGFLSKRRSITDDTIETSAPLS*TSPIS 181
           P+T    P+S+  SS +T   +S SS   S+ S F S   +I+     +S P S  S +S
Sbjct: 519 PSTSSSTPSSSIISSPMTSVLSSSSSIPTSSSSDFSSSITTISSGISSSSIP-STFSSVS 577

Query: 180 KPMISSFSVDNACCIQTKATSDDKRTFCSIS 88
             + SS S  ++  +   ++S    TF S S
Sbjct: 578 SILSSSTSSPSSTSLSISSSSTSS-TFSSAS 607


>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 800

 Score = 26.6 bits (56), Expect = 3.1
 Identities = 22/69 (31%), Positives = 34/69 (49%), Gaps = 1/69 (1%)
 Frame = -3

Query: 321 NSSALTVA*TSCSSDISTRSGFLSKRRSITDDTIETSAPLS*TSPISKPMISSFSVD-NA 145
           NSS L    TSC++  S  +G  S   +    T+  ++  S + PI  P  S+ S D N+
Sbjct: 218 NSSPLPTTSTSCTTSTSIPTGGSSSLSTPITPTVPPTSTSSTSIPI--PPTSTSSTDTNS 275

Query: 144 CCIQTKATS 118
             + T +TS
Sbjct: 276 SPLPTTSTS 284


>SPCC132.01c ||SPCC1322.17c|DUF814 family
           protein|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1021

 Score = 25.4 bits (53), Expect = 7.2
 Identities = 16/51 (31%), Positives = 29/51 (56%)
 Frame = +2

Query: 203 NGALVSIVSSVILRRLDKKPDRVEISEEQLVQATVRAEELAAEVGQPLRVV 355
           N  LV  + S I   L++  D ++I  E+L+Q+  R   +AA +  PL+++
Sbjct: 399 NPELVEAIISYINSLLNQGMDWLDI--EKLIQSQKRRSPVAAAIQIPLKLI 447


>SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gcn2
            |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1576

 Score = 25.0 bits (52), Expect = 9.5
 Identities = 12/30 (40%), Positives = 18/30 (60%)
 Frame = +3

Query: 558  EIEMEIVTNTDSLLTNNFETLTQLPTILKE 647
            EI  +I TN+D+L   + ET+  L  +L E
Sbjct: 1080 EISFDITTNSDNLDWYDAETIKALDEVLTE 1109


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,269,763
Number of Sequences: 5004
Number of extensions: 40051
Number of successful extensions: 100
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 98
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 99
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 293780908
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)