Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fprWP10_F_J07
(637 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 52 9e-08
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 44 2e-05
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 42 6e-05
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 31 0.18
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 27 2.3
SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy... 26 5.2
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 51.6 bits (118), Expect = 9e-08
Identities = 25/65 (38%), Positives = 39/65 (60%)
Frame = +3
Query: 315 EDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAA 494
E+ E K++DK+ NG + ELTH L +LGE+L EVA++ ++ D DG+I Y
Sbjct: 85 EEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREA--DTDGDGVINYEE 142
Query: 495 FLKKV 509
F + +
Sbjct: 143 FSRVI 147
Score = 31.1 bits (67), Expect = 0.14
Identities = 17/50 (34%), Positives = 29/50 (58%), Gaps = 2/50 (4%)
Frame = +2
Query: 65 MSDLSKNDVERASF--AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATI 208
M+ + D + A F AFS++D + G I + LG ++R+L +PT A +
Sbjct: 1 MTTRNLTDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAEL 50
Score = 29.9 bits (64), Expect = 0.32
Identities = 18/68 (26%), Positives = 36/68 (52%)
Frame = +3
Query: 318 DFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAF 497
+F E L+D++++G + EL + +LG+ +E+ ++ + D +G I + F
Sbjct: 13 EFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEV--DADGNGTIDFTEF 70
Query: 498 LKKVMAXK 521
L +MA K
Sbjct: 71 L-TMMARK 77
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 43.6 bits (98), Expect = 2e-05
Identities = 22/67 (32%), Positives = 41/67 (61%)
Frame = +3
Query: 315 EDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAA 494
E+F++ +++DK+ G++ EL + L +LGEKL + E+ E+ K DGM+ Y
Sbjct: 77 EEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGV---PVKDGMVNYHD 133
Query: 495 FLKKVMA 515
F++ ++A
Sbjct: 134 FVQMILA 140
Score = 42.3 bits (95), Expect = 6e-05
Identities = 19/34 (55%), Positives = 24/34 (70%)
Frame = +2
Query: 107 AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATI 208
AFS++D G G+I ++GDLLRA NPTLA I
Sbjct: 11 AFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEI 44
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 42.3 bits (95), Expect = 6e-05
Identities = 20/67 (29%), Positives = 41/67 (61%)
Frame = +3
Query: 315 EDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAA 494
E++++ +++DK+ +G + A+ + LGEKL D+EV + ++ DP + G Y
Sbjct: 78 EEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEA-DP-TNSGSFDYYD 135
Query: 495 FLKKVMA 515
F++++MA
Sbjct: 136 FVQRIMA 142
Score = 30.7 bits (66), Expect = 0.18
Identities = 14/60 (23%), Positives = 34/60 (56%)
Frame = +3
Query: 315 EDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAA 494
++ E LYD +++GL+ + + L +LG + D+E+A+++ + D D+ + + +
Sbjct: 9 DEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKFMSFVS 68
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 30.7 bits (66), Expect = 0.18
Identities = 18/74 (24%), Positives = 34/74 (45%)
Frame = +3
Query: 228 RRRARSCSHSKSSFPSXXXXXXXXXXXXYEDFLECLKLYDKNENGLMLGAELTHTLLALG 407
+RR+R+ S + + +D E KL+D +++ + EL + ALG
Sbjct: 8 KRRSRASSPTPARLGGYAPLRVEITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALG 67
Query: 408 EKLDDSEVAEVTKD 449
+ SEV ++ +D
Sbjct: 68 FNAEKSEVLKILRD 81
Score = 26.6 bits (56), Expect = 3.0
Identities = 12/39 (30%), Positives = 21/39 (53%)
Frame = +2
Query: 74 LSKNDVERASFAFSIYDFEGKGKIDAFNLGDLLRALNSN 190
+ ++ +E AF ++D + GKI NL + + LN N
Sbjct: 104 VERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNEN 142
Score = 26.6 bits (56), Expect = 3.0
Identities = 17/66 (25%), Positives = 29/66 (43%)
Frame = +3
Query: 315 EDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAA 494
E+ +L+D +E G + L L E +DD E+ + ++ D DG I
Sbjct: 110 EEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEF--DLDQDGEINEQE 167
Query: 495 FLKKVM 512
F+ +M
Sbjct: 168 FIAIMM 173
>SPBC215.01 ||SPBC3B9.20|GTPase activating
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 27.1 bits (57), Expect = 2.3
Identities = 13/29 (44%), Positives = 18/29 (62%), Gaps = 1/29 (3%)
Frame = +2
Query: 65 MSDLSKNDVER-ASFAFSIYDFEGKGKID 148
+++L DV R SF F +YDF G G +D
Sbjct: 605 IAELKFRDVMRNISFIFELYDFNGDGFMD 633
>SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1142
Score = 25.8 bits (54), Expect = 5.2
Identities = 9/22 (40%), Positives = 14/22 (63%)
Frame = +3
Query: 402 LGEKLDDSEVAEVTKDCMDPED 467
L EK+ D + + DC+DP+D
Sbjct: 777 LAEKVKDFQTMVILLDCLDPKD 798
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,192,869
Number of Sequences: 5004
Number of extensions: 36590
Number of successful extensions: 115
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 106
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 115
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 283719918
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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