Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fprWP09_F_J18
(655 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC17G6.16c |ysh1||mRNA cleavage and polyadenylation specificit... 94 1e-20
SPAC227.15 |||protein phosphatase regulatory subunit Reg1 |Schiz... 31 0.15
SPCC777.06c |||hydrolase |Schizosaccharomyces pombe|chr 3|||Manual 30 0.25
SPBC1709.15c |cft2||cleavage factor two Cft2/polyadenylation fac... 28 1.4
SPAC15A10.11 |ubr11||N-end-recognizing protein |Schizosaccharomy... 27 3.1
SPAC22A12.01c |pso2|snm1, SPAC56F8.17c, snm1|DNA 5' exonuclease ... 25 7.2
SPBC13G1.02 |||mannose-1-phosphate guanyltransferase |Schizosacc... 25 7.2
SPAC1D4.10 |||tRNA endonuclease|Schizosaccharomyces pombe|chr 1|... 25 9.5
>SPAC17G6.16c |ysh1||mRNA cleavage and polyadenylation specificity
factor complex subunit Ysh1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 775
Score = 94.3 bits (224), Expect = 1e-20
Identities = 45/103 (43%), Positives = 61/103 (59%)
Frame = +2
Query: 293 LGAGQDVGRSCILLTMGGKNIMLDCGMHMGYNDERRFPDFSYIVPEGPITSQIDCVIISH 472
LGAG +VGRSC ++ GK +MLD G+H Y P F S +D ++ISH
Sbjct: 42 LGAGNEVGRSCHVIQYKGKTVMLDAGVHPAYTGLSALPFFDEFD-----LSTVDVLLISH 96
Query: 473 FHLDHCGALPYMSEMIGYTGPIYMTHPTKAIAPILLEDMRKVA 601
FHLDH +LPY+ + + G ++MTHPTKA+ LL D KV+
Sbjct: 97 FHLDHVASLPYVMQKTNFRGRVFMTHPTKAVCKWLLSDYVKVS 139
>SPAC227.15 |||protein phosphatase regulatory subunit Reg1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 873
Score = 31.1 bits (67), Expect = 0.15
Identities = 15/42 (35%), Positives = 21/42 (50%)
Frame = +1
Query: 520 WLYRPYLHDTPD*SHSTNPSRGYEESRSGAQR*VKLLHFPND 645
WLY P LH + H ++ + E+ +S A R L H ND
Sbjct: 364 WLYGPLLHTSMPSRHHSHRKKEKEKEKSKAPRVTPLTHKTND 405
>SPCC777.06c |||hydrolase |Schizosaccharomyces pombe|chr 3|||Manual
Length = 301
Score = 30.3 bits (65), Expect = 0.25
Identities = 21/74 (28%), Positives = 34/74 (45%), Gaps = 4/74 (5%)
Frame = +2
Query: 347 KNIMLDCGMHMGYNDERRFPDFSYIVPEGPITSQIDCVIISHFHLDHCGALPYMSE-MIG 523
KNI++DCG H + + F + +D VI++H H D + + E +G
Sbjct: 66 KNILIDCGKHFYVSALKHFVEHK--------IRYLDAVILTHDHADAINGMDDLREWTLG 117
Query: 524 YTGP---IYMTHPT 556
+ P IY+T T
Sbjct: 118 FLQPSVKIYLTERT 131
>SPBC1709.15c |cft2||cleavage factor two Cft2/polyadenylation factor
CPSF-73 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 797
Score = 27.9 bits (59), Expect = 1.4
Identities = 27/73 (36%), Positives = 34/73 (46%), Gaps = 1/73 (1%)
Frame = +2
Query: 341 GGKNIMLDCGMHMGYNDERRFPDFSYIVPEGPITSQIDCVIISHFHLDHCGALPYMSEMI 520
G +I LD G+H+ Y D D S PE P Q D +++SH L H G L Y
Sbjct: 14 GTSHIELD-GIHI-YIDPGS--DDSLKHPEVP--EQPDLILLSHSDLAHIGGLVYAYYKY 67
Query: 521 GYTGP-IYMTHPT 556
+ IY T PT
Sbjct: 68 DWKNAYIYATLPT 80
>SPAC15A10.11 |ubr11||N-end-recognizing protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2052
Score = 26.6 bits (56), Expect = 3.1
Identities = 19/76 (25%), Positives = 35/76 (46%), Gaps = 1/76 (1%)
Frame = -2
Query: 381 PICIPQSNIMFLPPMVSKMQLRPTSWPAPSGVI-LISGIISFTTSKEAYENIRFKIMNIN 205
P+C SN++ P + KM L + P + + I + ++S Y+ +R + +
Sbjct: 1471 PLCRSLSNVLLPMPQIPKMCLNIDTLNFPRSMNGWLEEIGTMSSSSFEYQLVRSSLSDTK 1530
Query: 204 DTK*FASRXPWCINNQ 157
DT PW IN++
Sbjct: 1531 DTFRSCFLRPW-INSK 1545
>SPAC22A12.01c |pso2|snm1, SPAC56F8.17c, snm1|DNA 5' exonuclease
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 560
Score = 25.4 bits (53), Expect = 7.2
Identities = 13/36 (36%), Positives = 18/36 (50%)
Frame = +2
Query: 449 IDCVIISHFHLDHCGALPYMSEMIGYTGPIYMTHPT 556
++ +SHFH DH G L + GPIY + T
Sbjct: 208 VEAYFLSHFHSDHYGGLTPKWK----HGPIYCSEVT 239
>SPBC13G1.02 |||mannose-1-phosphate guanyltransferase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 414
Score = 25.4 bits (53), Expect = 7.2
Identities = 9/31 (29%), Positives = 15/31 (48%)
Frame = +2
Query: 452 DCVIISHFHLDHCGALPYMSEMIGYTGPIYM 544
DC++ + +C LP+ +G G I M
Sbjct: 384 DCIVHDEVRVQNCLVLPHKEIKVGLVGEIVM 414
>SPAC1D4.10 |||tRNA endonuclease|Schizosaccharomyces pombe|chr
1|||Manual
Length = 809
Score = 25.0 bits (52), Expect = 9.5
Identities = 16/48 (33%), Positives = 23/48 (47%), Gaps = 1/48 (2%)
Frame = +2
Query: 347 KNIMLDCGMHMGYNDERRFPD-FSYIVPEGPITSQIDCVIISHFHLDH 487
KNI+LDCG R++ D Y + + + + ISH H DH
Sbjct: 538 KNILLDCGEGTLGRLSRQYGDNLKYEI------ASLRWIYISHMHADH 579
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,747,291
Number of Sequences: 5004
Number of extensions: 56983
Number of successful extensions: 127
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 123
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 126
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 295793106
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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