BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fprWP09_F_E03
(602 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1006.07 |||translation initiation factor eIF4A|Schizosacchar... 28 0.91
SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccha... 27 1.6
SPBC1271.01c |pof13||F-box protein Pof13|Schizosaccharomyces pom... 26 3.7
SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 25 6.4
SPBC3D6.16 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 25 6.4
SPAC27E2.09 |mak2|phk1|histidine kinase Mak2 |Schizosaccharomyce... 25 6.4
SPAC6B12.09 |trm10||tRNA m|Schizosaccharomyces pombe|chr 1|||Manual 25 6.4
>SPAC1006.07 |||translation initiation factor
eIF4A|Schizosaccharomyces pombe|chr 1|||Manual
Length = 392
Score = 28.3 bits (60), Expect = 0.91
Identities = 9/21 (42%), Positives = 17/21 (80%)
Frame = +1
Query: 298 AIDLLTNDECRLLLEVEDFFN 360
+I+ +TND+ R++ E+E F+N
Sbjct: 358 SINFVTNDDVRMMREIEQFYN 378
>SPAC1834.01 |sup45||translation release factor
eRF1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 433
Score = 27.5 bits (58), Expect = 1.6
Identities = 12/30 (40%), Positives = 16/30 (53%)
Frame = +2
Query: 431 STRHHRRS*NQVPPNSLTIFCSS*TVNANK 520
STR + N+VP N L I+C + NK
Sbjct: 74 STRERLKLYNKVPDNGLVIYCGEVIMEGNK 103
>SPBC1271.01c |pof13||F-box protein Pof13|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 396
Score = 26.2 bits (55), Expect = 3.7
Identities = 24/85 (28%), Positives = 36/85 (42%), Gaps = 2/85 (2%)
Frame = +3
Query: 60 FSCNFAITMGF*YLIVCTPACAFTHN-IKMLAKMAPS-QDKLVHLHKTPTSLDINPSGLL 233
F C I++ F I A A H I L + P +D H K I+P LL
Sbjct: 310 FVCPSCISLDFDLQI---QAFARQHRVISTLGVVYPEREDSCFHKIKAAQWHQISPRSLL 366
Query: 234 FAFVELNHYNNECESEGVMGCGHRF 308
F F E NH +++ ++ G ++
Sbjct: 367 FQFQEQNHIHHKKIRRKLLAAGWKW 391
>SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 583
Score = 25.4 bits (53), Expect = 6.4
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = +1
Query: 316 NDECRLLLEVEDFFNDDC 369
NDECR L + FF+ +C
Sbjct: 535 NDECRRLKQCNHFFHREC 552
>SPBC3D6.16 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 95
Score = 25.4 bits (53), Expect = 6.4
Identities = 12/29 (41%), Positives = 14/29 (48%)
Frame = +3
Query: 171 DKLVHLHKTPTSLDINPSGLLFAFVELNH 257
D + H K +S NPS LLF L H
Sbjct: 59 DGIPHSRKKVSSAHFNPSTLLFLLKRLGH 87
>SPAC27E2.09 |mak2|phk1|histidine kinase Mak2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2310
Score = 25.4 bits (53), Expect = 6.4
Identities = 14/49 (28%), Positives = 23/49 (46%)
Frame = -3
Query: 441 WRVXTRSVIDFDLRCSARKVFKQITIIIEEILNFEQQTALIVSEQIDGR 295
W T+ V R S RKV + + + EE +++ ALI + + R
Sbjct: 2143 WSTPTKLVEPSQFRASPRKVDQAVVLSSEEKEILQKKYALIAEDNLIAR 2191
>SPAC6B12.09 |trm10||tRNA m|Schizosaccharomyces pombe|chr 1|||Manual
Length = 304
Score = 25.4 bits (53), Expect = 6.4
Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 4/49 (8%)
Frame = +3
Query: 147 LAKMAPSQDKLVHLH----KTPTSLDINPSGLLFAFVELNHYNNECESE 281
L + ++KLV+L T T LD + ++ A V+ N Y N C+++
Sbjct: 170 LEEFESQKEKLVYLSADSDNTITELDEDKIYIIGAIVDKNRYKNLCQNK 218
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,097,025
Number of Sequences: 5004
Number of extensions: 38035
Number of successful extensions: 86
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 85
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 86
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 264253462
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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