Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fprWP08_F_G10
(655 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 127 2e-30
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 80 3e-16
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 80 3e-16
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 80 3e-16
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 70 3e-13
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 34 0.016
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 31 0.11
SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit... 30 0.34
SPBC557.05 |||arrestin|Schizosaccharomyces pombe|chr 2|||Manual 30 0.34
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 30 0.34
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 29 0.77
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 29 0.77
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 28 1.0
SPAC8E11.08c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual 28 1.0
SPAP27G11.02 |||TPR repeat protein, unknown biological role|Schi... 27 2.4
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 27 3.1
SPBC9B6.10 |cdc37||Hsp90 co-chaperone Cdc37|Schizosaccharomyces ... 26 4.1
SPBC1734.01c ||SPBC337.17c|RNA-binding protein|Schizosaccharomyc... 25 9.5
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 25 9.5
SPCC5E4.05c |||serine hydrolase |Schizosaccharomyces pombe|chr 3... 25 9.5
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 127 bits (306), Expect = 2e-30
Identities = 56/71 (78%), Positives = 64/71 (90%)
Frame = +2
Query: 443 KEHVNVVFIGHVDAGKSTIGGQIMSLTGMVDKRTLEKYEREAREKSRESWYLSWALDTNQ 622
KEHVN+VFIGHVDAGKST+GG I+ LTGMVDKRT+EK EREA+E +ESWYLSWALD+
Sbjct: 236 KEHVNIVFIGHVDAGKSTLGGNILFLTGMVDKRTMEKIEREAKEAGKESWYLSWALDSTS 295
Query: 623 EERDKGKTVEV 655
EER+KGKTVEV
Sbjct: 296 EEREKGKTVEV 306
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 79.8 bits (188), Expect = 3e-16
Identities = 34/72 (47%), Positives = 52/72 (72%)
Frame = +2
Query: 440 KKEHVNVVFIGHVDAGKSTIGGQIMSLTGMVDKRTLEKYEREAREKSRESWYLSWALDTN 619
+K H+NVV IGHVD+GKST G ++ G +DKRT+EK+E+EA E + S+ +W LD
Sbjct: 4 EKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVLDKL 63
Query: 620 QEERDKGKTVEV 655
+ ER++G T+++
Sbjct: 64 KAERERGITIDI 75
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 79.8 bits (188), Expect = 3e-16
Identities = 34/72 (47%), Positives = 52/72 (72%)
Frame = +2
Query: 440 KKEHVNVVFIGHVDAGKSTIGGQIMSLTGMVDKRTLEKYEREAREKSRESWYLSWALDTN 619
+K H+NVV IGHVD+GKST G ++ G +DKRT+EK+E+EA E + S+ +W LD
Sbjct: 4 EKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVLDKL 63
Query: 620 QEERDKGKTVEV 655
+ ER++G T+++
Sbjct: 64 KAERERGITIDI 75
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 79.8 bits (188), Expect = 3e-16
Identities = 34/72 (47%), Positives = 52/72 (72%)
Frame = +2
Query: 440 KKEHVNVVFIGHVDAGKSTIGGQIMSLTGMVDKRTLEKYEREAREKSRESWYLSWALDTN 619
+K H+NVV IGHVD+GKST G ++ G +DKRT+EK+E+EA E + S+ +W LD
Sbjct: 4 EKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVLDKL 63
Query: 620 QEERDKGKTVEV 655
+ ER++G T+++
Sbjct: 64 KAERERGITIDI 75
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 70.1 bits (164), Expect = 3e-13
Identities = 36/94 (38%), Positives = 57/94 (60%), Gaps = 6/94 (6%)
Frame = +2
Query: 392 KKIPKKKP------PRVEDTRSKKEHVNVVFIGHVDAGKSTIGGQIMSLTGMVDKRTLEK 553
KKI K+ P P + + + K V++V GHVD+GKST+ G+IM G ++ R+++K
Sbjct: 152 KKIKKQNPTDLVSVPEIFEQSNPKPVVHLVVTGHVDSGKSTMLGRIMFELGEINSRSMQK 211
Query: 554 YEREAREKSRESWYLSWALDTNQEERDKGKTVEV 655
EA + S+ +W LDT +EER +G T++V
Sbjct: 212 LHNEAANSGKGSFSYAWLLDTTEEERARGVTMDV 245
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 34.3 bits (75), Expect = 0.016
Identities = 19/51 (37%), Positives = 27/51 (52%), Gaps = 3/51 (5%)
Frame = +2
Query: 440 KKEHVNVVFIGHVDAGKSTIGGQI---MSLTGMVDKRTLEKYEREAREKSR 583
KK HVN+ IGHVD GK+T+ I +S G + ++ EK+R
Sbjct: 50 KKPHVNIGTIGHVDHGKTTLTAAITKCLSDLGQASFMDYSQIDKAPEEKAR 100
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 31.5 bits (68), Expect = 0.11
Identities = 17/44 (38%), Positives = 25/44 (56%), Gaps = 5/44 (11%)
Frame = +2
Query: 455 NVVFIGHVDAGKSTIGGQIMSLTGMVDKRT-----LEKYEREAR 571
N I H+D GKST+ I+ LTG++++ L+K E E R
Sbjct: 60 NWAVIAHIDHGKSTLSDCILKLTGVINEHNFRNQFLDKLEVERR 103
>SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit
Gpa2 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 354
Score = 29.9 bits (64), Expect = 0.34
Identities = 16/45 (35%), Positives = 26/45 (57%), Gaps = 1/45 (2%)
Frame = +2
Query: 389 TKKIPKKKPPRVEDTRSKKEHV-NVVFIGHVDAGKSTIGGQIMSL 520
+KK+ K ++E+ K + + V+ +G D+GKSTI QI L
Sbjct: 13 SKKLNSKIEKQIENASKKDKKIYKVLLLGASDSGKSTISKQIKIL 57
>SPBC557.05 |||arrestin|Schizosaccharomyces pombe|chr 2|||Manual
Length = 433
Score = 29.9 bits (64), Expect = 0.34
Identities = 14/37 (37%), Positives = 20/37 (54%)
Frame = +1
Query: 421 SRRYTKQKRTCKCSIYRTCRCRKVDYWWSNNVTHWNG 531
SR+ ++ +TCKC T RC K +N +WNG
Sbjct: 289 SRKNSEVSKTCKCLQKETIRCNK-----KSNCYNWNG 320
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 29.9 bits (64), Expect = 0.34
Identities = 11/49 (22%), Positives = 27/49 (55%), Gaps = 1/49 (2%)
Frame = +2
Query: 398 IPKKKPPRVEDTRSKKEHV-NVVFIGHVDAGKSTIGGQIMSLTGMVDKR 541
+P P ++ + +E++ N + HVD GK+T+ +++ G++ +
Sbjct: 1 MPVIPPEKLVSLQKNQENIRNFTLLAHVDHGKTTLADSLLASNGIISSK 49
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 28.7 bits (61), Expect = 0.77
Identities = 14/41 (34%), Positives = 22/41 (53%), Gaps = 1/41 (2%)
Frame = +2
Query: 413 PPRVEDTRSKKEHV-NVVFIGHVDAGKSTIGGQIMSLTGMV 532
P V + K +V N+ I HVD GKST+ ++ G++
Sbjct: 6 PEEVRNLMGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGII 46
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 28.7 bits (61), Expect = 0.77
Identities = 14/41 (34%), Positives = 22/41 (53%), Gaps = 1/41 (2%)
Frame = +2
Query: 413 PPRVEDTRSKKEHV-NVVFIGHVDAGKSTIGGQIMSLTGMV 532
P V + K +V N+ I HVD GKST+ ++ G++
Sbjct: 6 PEEVRNLMGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGII 46
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 28.3 bits (60), Expect = 1.0
Identities = 20/75 (26%), Positives = 36/75 (48%)
Frame = +2
Query: 428 DTRSKKEHVNVVFIGHVDAGKSTIGGQIMSLTGMVDKRTLEKYEREAREKSRESWYLSWA 607
D + K+ N+ H+D+GK+T +++ TG + K E R K +
Sbjct: 52 DKKRLKQIRNIGISAHIDSGKTTFTERVLYYTGRI------KDIHEVRGKDN----VGAK 101
Query: 608 LDTNQEERDKGKTVE 652
+D + ER+KG T++
Sbjct: 102 MDFMELEREKGITIQ 116
>SPAC8E11.08c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual
Length = 100
Score = 28.3 bits (60), Expect = 1.0
Identities = 12/16 (75%), Positives = 12/16 (75%)
Frame = -2
Query: 90 NNLVFFPG*LKLFRFC 43
NNLVFF G LFRFC
Sbjct: 85 NNLVFFMGKCDLFRFC 100
>SPAP27G11.02 |||TPR repeat protein, unknown biological
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 356
Score = 27.1 bits (57), Expect = 2.4
Identities = 14/48 (29%), Positives = 27/48 (56%), Gaps = 3/48 (6%)
Frame = +2
Query: 491 STIGGQIMSLTGMVDKR---TLEKYEREAREKSRESWYLSWALDTNQE 625
+ + +++ T VDK+ TL K + +K+ +S+Y ++ D NQE
Sbjct: 237 NNLATSLLAQTETVDKKHHETLMKQSQSWSQKAVDSYYFAYPKDRNQE 284
>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 26.6 bits (56), Expect = 3.1
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = +2
Query: 437 SKKEHVNVVFIGHVDAGKSTIGGQIMSLTGMVDKRTLEK 553
S++ +N+ IGHV GKST+ I + + K LE+
Sbjct: 19 SRQATINIGTIGHVAHGKSTVVKAISGVHTVRFKNELER 57
>SPBC9B6.10 |cdc37||Hsp90 co-chaperone Cdc37|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 466
Score = 26.2 bits (55), Expect = 4.1
Identities = 15/60 (25%), Positives = 29/60 (48%), Gaps = 1/60 (1%)
Frame = +2
Query: 398 IPKKKPPRVEDTRSKKEHVNVV-FIGHVDAGKSTIGGQIMSLTGMVDKRTLEKYEREARE 574
+ KK+P + + T++KKE + V+ + + G + + TG K + + E E E
Sbjct: 183 VQKKEPEKPKKTKTKKETIQVIESLNNPTPPTDFPGAKEQASTGNAPKNPVNENESEDEE 242
>SPBC1734.01c ||SPBC337.17c|RNA-binding protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 682
Score = 25.0 bits (52), Expect = 9.5
Identities = 12/41 (29%), Positives = 24/41 (58%)
Frame = +2
Query: 533 DKRTLEKYEREAREKSRESWYLSWALDTNQEERDKGKTVEV 655
D+ T+EKY+R+A E+ + L L +++ +G V++
Sbjct: 471 DETTIEKYKRKAAERKQRRKELR-QLKKTKDDEGEGSDVDL 510
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 25.0 bits (52), Expect = 9.5
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = +2
Query: 455 NVVFIGHVDAGKSTIGGQIMSLTG 526
NV I H+DAGK+T+ +++ G
Sbjct: 30 NVGIIAHIDAGKTTLTEKMLYYGG 53
>SPCC5E4.05c |||serine hydrolase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 378
Score = 25.0 bits (52), Expect = 9.5
Identities = 12/29 (41%), Positives = 14/29 (48%)
Frame = +2
Query: 233 VEEPPPTTASVPPDVSPTADSWEVEADDA 319
VE P PT + P SPT E E+ A
Sbjct: 288 VEHPKPTATTSAPSASPTGVPVEEESHKA 316
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,035,257
Number of Sequences: 5004
Number of extensions: 30844
Number of successful extensions: 146
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 143
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 145
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 295793106
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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