Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fprWP08_F_E22
         (654 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce...    75   7e-15
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi...    52   7e-08
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch...    37   0.003
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb...    33   0.036
SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces pomb...    30   0.34 
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces...    29   0.77 
SPAC2E1P3.05c |||fungal cellulose binding domain protein|Schizos...    28   1.4  
SPCC970.08 |||inositol polyphosphate kinase |Schizosaccharomyces...    27   1.8  
SPBC887.12 |||P-type ATPase |Schizosaccharomyces pombe|chr 2|||M...    27   3.1  
SPAP27G11.04c |||tRNA specific adenosine deaminase subunit Tad3 ...    26   4.1  
SPBC13G1.10c |mug81||ATP-dependent RNA helicase Slh1|Schizosacch...    26   4.1  
SPAC25H1.07 |||DUF1620 family protein|Schizosaccharomyces pombe|...    26   5.5  
SPCC18.01c |adg3|SPCC74.07c|beta-glucosidase Adg3 |Schizosacchar...    25   9.5  

>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 174

 Score = 75.4 bits (177), Expect = 7e-15
 Identities = 47/175 (26%), Positives = 85/175 (48%)
 Frame = +3

Query: 45  MGQGKSQFTEEELQDYEDLTYFTKKEVLYAHQKFKALAPEKVGHNKNAKLPMAKVLTYPE 224
           MGQ +SQ  E+ + +    + F+ +E+    ++F      K+  N++  +   + L+ P 
Sbjct: 1   MGQSQSQIFEDLISN----SSFSNEEIERIRKRFI-----KIDANQSGSIDRNEFLSIPS 51

Query: 225 LKVNPFKDRICKVFSSSNDGDCTFEDFLDMMSVFSEMAPKAVKAEHAFRIFDFDGDDMIG 404
           +  NP   R+  V      GD  F++F++ +SVFS    K  K + AF+I+D D D  I 
Sbjct: 52  VASNPLASRLFSVVDEDGGGDVDFQEFINSLSVFSVHGNKEEKLKFAFKIYDIDRDGYIS 111

Query: 405 VSDLREVIQRLCGPELKLSDSEIQQLVQNVXXXXXXXXXXXXSFAEFEHIIDKSS 569
             +L  V++ + G  L+  + ++QQ+V               SF EF+ I+  S+
Sbjct: 112 NGELYLVLKMMVGTNLR--EDQLQQIVDKTIMEVDKDRDGKISFEEFKDIVSGSN 164


>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
           Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 190

 Score = 52.0 bits (119), Expect = 7e-08
 Identities = 37/147 (25%), Positives = 73/147 (49%), Gaps = 5/147 (3%)
 Frame = +3

Query: 45  MGQGKSQFTEEELQDYEDLTYFTKKEVLYAHQKFKALAPEKVGHNKNAKLPMAKVLTYPE 224
           MG+ +S+ ++++LQD    T F KKE+   ++ F    P   GH   ++        +P 
Sbjct: 1   MGKSQSKLSQDQLQDLVRSTRFDKKELQQWYKGFFKDCPS--GHLNKSEFQKIYKQFFPF 58

Query: 225 LKVNPFKDRICKVFSSSNDGDCTFEDFLDMMSVFS--EMAPKAVKAEHAFRIFDFDGDDM 398
              + F + +  VF +  +G   F++F+  +SV S  E+  K +    AF+++D D + +
Sbjct: 59  GDPSAFAEYVFNVFDADKNGYIDFKEFICALSVTSRGELNDKLI---WAFQLYDLDNNGL 115

Query: 399 IGVSDLREV---IQRLCGPELKLSDSE 470
           I   ++  +   I ++ G  +KL + E
Sbjct: 116 ISYDEMLRIVDAIYKMVGSMVKLPEDE 142


>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 150

 Score = 36.7 bits (81), Expect = 0.003
 Identities = 25/82 (30%), Positives = 42/82 (51%)
 Frame = +3

Query: 243 KDRICKVFSSSNDGDCTFEDFLDMMSVFSEMAPKAVKAEHAFRIFDFDGDDMIGVSDLRE 422
           +D I +V +  N G   F +FL MM+   +      +   AF++FD DG+  I V +L  
Sbjct: 51  QDMINEVDADGN-GTIDFTEFLTMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTH 109

Query: 423 VIQRLCGPELKLSDSEIQQLVQ 488
           V+  L G   +LS  E+  +++
Sbjct: 110 VLTSL-GE--RLSQEEVADMIR 128


>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 141

 Score = 33.1 bits (72), Expect = 0.036
 Identities = 19/43 (44%), Positives = 28/43 (65%)
 Frame = +3

Query: 366 FRIFDFDGDDMIGVSDLREVIQRLCGPELKLSDSEIQQLVQNV 494
           F++FD D   MIGV +LR V+  L G   KLS+ E+ +L++ V
Sbjct: 83  FQVFDKDATGMIGVGELRYVLTSL-GE--KLSNEEMDELLKGV 122


>SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 262

 Score = 29.9 bits (64), Expect = 0.34
 Identities = 30/109 (27%), Positives = 49/109 (44%), Gaps = 3/109 (2%)
 Frame = -3

Query: 472 ISESDSFNSGPHRRCITSRKSDTPIMSSPSKSNILNACSAFTALGAISLKTDIMSKKSSK 293
           I  S S +S        S  S T   SS S S  ++  S+ T+  + S  + + S  +S 
Sbjct: 133 IYSSTSASSTSSSTATPSSSSTTSSSSSSSSSTPIS--SSITSSISSSASSSVSSSSASS 190

Query: 292 VQSPSLLELNTLHILS---LNGFTLSSGYVSTLAIGNLAFLLCPTFSGA 155
             S S  +  T+   S   ++GF+ S+   S+ A GN +     ++SGA
Sbjct: 191 SGSISSADAKTVSASSNSTISGFSTSTTSASSSAAGNSSSSSYTSYSGA 239


>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 143

 Score = 28.7 bits (61), Expect = 0.77
 Identities = 17/42 (40%), Positives = 25/42 (59%)
 Frame = +3

Query: 363 AFRIFDFDGDDMIGVSDLREVIQRLCGPELKLSDSEIQQLVQ 488
           AFR+FD D    I  +   + ++ L G   KLSD+E+Q +VQ
Sbjct: 83  AFRVFDKDNSGYIETAKFADYMKTL-GE--KLSDNEVQLMVQ 121


>SPAC2E1P3.05c |||fungal cellulose binding domain
           protein|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 197

 Score = 27.9 bits (59), Expect = 1.4
 Identities = 21/93 (22%), Positives = 41/93 (44%)
 Frame = -3

Query: 481 SCCISESDSFNSGPHRRCITSRKSDTPIMSSPSKSNILNACSAFTALGAISLKTDIMSKK 302
           +CC   S+   +GP+         D    SS + S+  +  S+ + + + +L +   S  
Sbjct: 85  TCCEPGSECIYNGPYYSQCIPVDIDPSSSSSSAASSTTSTTSSSSLVSSTTLTSSSPSAV 144

Query: 301 SSKVQSPSLLELNTLHILSLNGFTLSSGYVSTL 203
           SS    PS+    +  + + +  +LSS   ST+
Sbjct: 145 SSTTSIPSISSTISSSVSTSSFTSLSSSGFSTV 177


>SPCC970.08 |||inositol polyphosphate kinase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 967

 Score = 27.5 bits (58), Expect = 1.8
 Identities = 18/69 (26%), Positives = 30/69 (43%)
 Frame = -3

Query: 481 SCCISESDSFNSGPHRRCITSRKSDTPIMSSPSKSNILNACSAFTALGAISLKTDIMSKK 302
           S  +SE+D   S               +  S + +N+    S  TAL  +S   + +S++
Sbjct: 133 SSAVSEADDMASAHSAHPSRKASRSLRLFESSTSNNLETGNSTNTALHNVSSPLESVSER 192

Query: 301 SSKVQSPSL 275
            SK  SPS+
Sbjct: 193 LSKSGSPSV 201


>SPBC887.12 |||P-type ATPase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 1258

 Score = 26.6 bits (56), Expect = 3.1
 Identities = 17/59 (28%), Positives = 28/59 (47%)
 Frame = -2

Query: 392 ITIKIKYPKCMFSFYCFRSHLTEN*HHVQEVFKGAISIITGTEHLTYPIFEWIHFKFWI 216
           +  +I +  C+F F CF S L    H  + V+  A+S +  T +     F+ +   FWI
Sbjct: 403 VNSQILFLLCIFVFLCFASSLGALIH--RSVYGSALSYVKYTSNRAGMFFKGL-LTFWI 458


>SPAP27G11.04c |||tRNA specific adenosine deaminase subunit Tad3
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 315

 Score = 26.2 bits (55), Expect = 4.1
 Identities = 17/49 (34%), Positives = 24/49 (48%)
 Frame = +3

Query: 57  KSQFTEEELQDYEDLTYFTKKEVLYAHQKFKALAPEKVGHNKNAKLPMA 203
           KS    E  +D+EDL   +K   LYA   F  L  E+  H  N+  P++
Sbjct: 94  KSMTANEIGKDFEDLGIVSKMIFLYAVPAFPPLTDEQF-HEWNSVWPVS 141


>SPBC13G1.10c |mug81||ATP-dependent RNA helicase
            Slh1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1935

 Score = 26.2 bits (55), Expect = 4.1
 Identities = 13/43 (30%), Positives = 22/43 (51%)
 Frame = -1

Query: 582  HGRNQNSYL*YVQTQQRRELHHHQDLLLPKHSAPVVVFLSLIV 454
            HG +Q  ++ +V+     E+ HH+ LLL K +      LS  +
Sbjct: 1022 HGNSQMFWI-FVEDSNGLEILHHEQLLLNKRNVSTSHLLSFTI 1063


>SPAC25H1.07 |||DUF1620 family protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 885

 Score = 25.8 bits (54), Expect = 5.5
 Identities = 13/27 (48%), Positives = 14/27 (51%)
 Frame = +3

Query: 54  GKSQFTEEELQDYEDLTYFTKKEVLYA 134
           GK+ F    L    DL Y TK E LYA
Sbjct: 29  GKNDFEISLLGKVNDLVYDTKNEYLYA 55


>SPCC18.01c |adg3|SPCC74.07c|beta-glucosidase Adg3
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1131

 Score = 25.0 bits (52), Expect = 9.5
 Identities = 27/102 (26%), Positives = 45/102 (44%)
 Frame = -3

Query: 406 TPIMSSPSKSNILNACSAFTALGAISLKTDIMSKKSSKVQSPSLLELNTLHILSLNGFTL 227
           TP+ SS   S    A    T++   S   D  S  +S +QS S +  ++   LS N  + 
Sbjct: 484 TPVTSSSIFSTATEATD--TSVQTSSAIYD--SSSTSNIQSSSSVYASSTGALSSNSLSS 539

Query: 226 SSGYVSTLAIGNLAFLLCPTFSGARALNFW*AYNTSFFVKYV 101
           S+  VST  I N +  +  + + A + N   +  +S    Y+
Sbjct: 540 STSSVSTSYIPNASSSVYASSTEALSSNSLSSSTSSASTSYI 581


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,625,039
Number of Sequences: 5004
Number of extensions: 52277
Number of successful extensions: 168
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 160
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 166
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 295793106
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)