Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fprWP08_F_D22
(654 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch... 176 2e-45
SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha... 124 1e-29
SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2... 103 2e-23
SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosacch... 101 1e-22
SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz... 91 1e-19
SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosac... 91 2e-19
SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosac... 77 3e-15
SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac... 72 6e-14
SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosacch... 71 1e-13
SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac... 71 2e-13
SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 65 1e-11
SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo... 64 1e-11
SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc... 44 1e-05
SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizos... 28 1.4
SPCC1183.05c |lig4||DNA ligase Lig4|Schizosaccharomyces pombe|ch... 27 2.4
SPCC162.08c |nup211||nuclear pore complex associated protein|Sch... 26 5.5
SPCC757.11c |||membrane transporter|Schizosaccharomyces pombe|ch... 25 7.2
SPAC222.07c |hri2||eIF2 alpha kinase Hri2|Schizosaccharomyces po... 25 7.2
SPAC31G5.15 |||phosphatidylserine decarboxylase |Schizosaccharom... 25 9.5
SPBC215.07c |||PWWP domain protein|Schizosaccharomyces pombe|chr... 25 9.5
>SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme
Ubc13|Schizosaccharomyces pombe|chr 1|||Manual
Length = 148
Score = 176 bits (429), Expect = 2e-45
Identities = 79/133 (59%), Positives = 95/133 (71%)
Frame = +1
Query: 31 RSXVPGISAVPSETNARYFHVIVTGPEDSXXXXXXXXXXXXXXXDYPMSAPKVRFITKIY 210
R PGI A P+E N RYF + + GP+ S +YPM P VRF+TKIY
Sbjct: 16 RDPPPGIVAAPTEDNLRYFKITMEGPQQSAYEGGKFHLELFLPDEYPMMPPNVRFLTKIY 75
Query: 211 HPNIDRLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAELWKVNESEA 390
HPN+D+LGRICL LK WSPALQIRTVLLSIQAL+ APNPDDPL NDVA++WK NE +A
Sbjct: 76 HPNVDKLGRICLSTLKKDWSPALQIRTVLLSIQALMGAPNPDDPLDNDVAKIWKENEPQA 135
Query: 391 IRNAKEWTRRYAM 429
I NA+EWT++YA+
Sbjct: 136 IANAREWTKKYAV 148
>SPBC119.02 |ubc4||ubiquitin conjugating enzyme
Ubc4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 147
Score = 124 bits (299), Expect = 1e-29
Identities = 59/126 (46%), Positives = 78/126 (61%)
Frame = +1
Query: 52 SAVPSETNARYFHVIVTGPEDSXXXXXXXXXXXXXXXDYPMSAPKVRFITKIYHPNIDRL 231
SA P + ++ + GP DS DYP PKV F T+IYHPNI+
Sbjct: 22 SAGPVGDDLFHWQATIMGPADSPYAGGVFFLSIHFPTDYPFKPPKVNFTTRIYHPNINSN 81
Query: 232 GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAELWKVNESEAIRNAKEW 411
G ICLDIL+D+WSPAL I VLLSI +LL+ PNPDDPL ++A ++K + S +A+EW
Sbjct: 82 GSICLDILRDQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHVYKTDRSRYELSAREW 141
Query: 412 TRRYAM 429
TR+YA+
Sbjct: 142 TRKYAI 147
>SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2-C
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 176
Score = 103 bits (247), Expect = 2e-23
Identities = 50/119 (42%), Positives = 68/119 (57%), Gaps = 1/119 (0%)
Frame = +1
Query: 34 SXVPGISAVP-SETNARYFHVIVTGPEDSXXXXXXXXXXXXXXXDYPMSAPKVRFITKIY 210
S PGISA P S++N ++ +TGP D+ +YP S P + F + ++
Sbjct: 43 SNTPGISAFPDSDSNLLHWAGTITGPSDTYYEGLKFKISMSFPANYPYSPPTIIFTSPMW 102
Query: 211 HPNIDRLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAELWKVNESE 387
HPN+D G ICLDILKDKWS ++T+LLS+Q+LL PN PL AELW + E
Sbjct: 103 HPNVDMSGNICLDILKDKWSAVYNVQTILLSLQSLLGEPNNASPLNAQAAELWSKDPIE 161
>SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme
Ubc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 217
Score = 101 bits (241), Expect = 1e-22
Identities = 46/109 (42%), Positives = 64/109 (58%), Gaps = 1/109 (0%)
Frame = +1
Query: 103 GPEDSXXXXXXXXXXXXXXXDYPMSAPKVRFITKIYHPNID-RLGRICLDILKDKWSPAL 279
GPE + DYP PK+ F TKIYHPN+ + G ICLDILKD+WSP
Sbjct: 42 GPEGTPYEGGYFVVDIEIPIDYPFRPPKMNFDTKIYHPNVSSQTGAICLDILKDQWSPVY 101
Query: 280 QIRTVLLSIQALLSAPNPDDPLANDVAELWKVNESEAIRNAKEWTRRYA 426
+++ L+S+Q+LL P P +P VA+++ N + +R A+EWT YA
Sbjct: 102 TMKSALISLQSLLCTPEPSNPQDAQVAQVYLQNYQQFVRTAREWTSSYA 150
>SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme
E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 151
Score = 91.5 bits (217), Expect = 1e-19
Identities = 39/121 (32%), Positives = 66/121 (54%)
Frame = +1
Query: 46 GISAVPSETNARYFHVIVTGPEDSXXXXXXXXXXXXXXXDYPMSAPKVRFITKIYHPNID 225
G+SA P N ++ ++ GP D+ YP P V+F++ ++HPN+
Sbjct: 23 GVSASPVSDNVMLWNAVIIGPADTPFEDGTFKLVLSFDEQYPNKPPLVKFVSTMFHPNVY 82
Query: 226 RLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAELWKVNESEAIRNAK 405
G +CLDIL+++WSP + +L SIQ+LL+ PN P + A+L + N+ E +R +
Sbjct: 83 ANGELCLDILQNRWSPTYDVAAILTSIQSLLNDPNNASPANAEAAQLHRENKKEYVRRVR 142
Query: 406 E 408
+
Sbjct: 143 K 143
>SPAC1250.03 |ubc14||ubiquitin conjugating enzyme
Ubc14|Schizosaccharomyces pombe|chr 1|||Manual
Length = 155
Score = 90.6 bits (215), Expect = 2e-19
Identities = 46/141 (32%), Positives = 72/141 (51%), Gaps = 1/141 (0%)
Frame = +1
Query: 7 SKRHSD*CRSXVPGISAVPSETNARYFHVIVTGPEDSXXXXXXXXXXXXXXXDYPMSAPK 186
+K +SD +P I + N ++ GP DS DYP P
Sbjct: 13 TKEYSDLREHPIPDIRVNLVDDNLFHWACTALGPSDSVYAGGKFHFSLKFPLDYPFQPPT 72
Query: 187 VRFITKIYHPNIDRLGRICLDILKDK-WSPALQIRTVLLSIQALLSAPNPDDPLANDVAE 363
+ F T+IYHPN D G +CL ILK + + P++++R+VL I LL PNPDDPL +AE
Sbjct: 73 IEFTTRIYHPNFDSEGNVCLAILKQQVFKPSIKLRSVLEQILQLLREPNPDDPLVASIAE 132
Query: 364 LWKVNESEAIRNAKEWTRRYA 426
++ + + A+++ ++A
Sbjct: 133 QYRNDRPSFDKIARDYVEQFA 153
>SPCC777.10c |ubc12||ubiquitin conjugating enzyme
Ubc12|Schizosaccharomyces pombe|chr 3|||Manual
Length = 177
Score = 76.6 bits (180), Expect = 3e-15
Identities = 30/66 (45%), Positives = 45/66 (68%)
Frame = +1
Query: 163 DYPMSAPKVRFITKIYHPNIDRLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDP 342
+YP PKV+ + KIYHPNID G +CL+IL+ W+P L + ++L+ +Q L +PN +DP
Sbjct: 79 NYPHDPPKVKCLNKIYHPNIDIEGNVCLNILRQDWNPVLNLNSILVGLQFLFLSPNAEDP 138
Query: 343 LANDVA 360
L + A
Sbjct: 139 LNKEAA 144
>SPBC1198.09 |ubc16||ubiquitin conjugating enzyme
Ubc16|Schizosaccharomyces pombe|chr 2|||Manual
Length = 160
Score = 72.1 bits (169), Expect = 6e-14
Identities = 40/120 (33%), Positives = 60/120 (50%), Gaps = 3/120 (2%)
Frame = +1
Query: 40 VPGISAVPSETNARYFH--VIVTGPEDSXXXXXXXXXXXXXXXDYPMSAPKVRFITKIYH 213
+ G+ + T+ FH ++ GP ++ YP+S P V F TKI H
Sbjct: 20 ISGLLLLKPVTDVDMFHWKAVIEGPTETPYEGGQWVLDIHVHEGYPISPPSVYFQTKIVH 79
Query: 214 PNIDRL-GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAELWKVNESEA 390
PNI G +C+DILK WSPA +++ L+I +LLS + PL D A+L + + A
Sbjct: 80 PNISWTNGEVCMDILKTHWSPAWSLQSACLAIISLLSNYDASSPLNVDAAKLLRTGDKTA 139
>SPBC211.07c |ubc8||ubiquitin conjugating enzyme
Ubc8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 71.3 bits (167), Expect = 1e-13
Identities = 35/120 (29%), Positives = 57/120 (47%), Gaps = 2/120 (1%)
Frame = +1
Query: 73 NARYFHVIVTGPEDSXXXXXXXXXXXXXXXDYPMSAPKVRFITKIYHPNIDRL-GRICLD 249
N + F+V GP ++ +YP +P + F+ +I+HPNID L G +CLD
Sbjct: 28 NMQEFYVRFHGPSETPYSGGIWKVHVELPSEYPWKSPSIGFVNRIFHPNIDELSGSVCLD 87
Query: 250 ILKDKWSPALQIRTVL-LSIQALLSAPNPDDPLANDVAELWKVNESEAIRNAKEWTRRYA 426
++ WSP + + + + LL PN DPL + A L + +++ RYA
Sbjct: 88 VINQTWSPMFDMINIFEVFLPQLLRYPNASDPLNGEAAALLLREPNTYYAKVRDYIARYA 147
>SPBC1105.09 |ubc15||ubiquitin conjugating enzyme
Ubc15|Schizosaccharomyces pombe|chr 2|||Manual
Length = 167
Score = 70.5 bits (165), Expect = 2e-13
Identities = 36/125 (28%), Positives = 62/125 (49%), Gaps = 13/125 (10%)
Frame = +1
Query: 85 FHVIVTGPEDSXXXXXXXXXXXXXXXDYPMSAPKVRFITKIYHPNIDRLGRICLDILK-- 258
+ V++ GPED+ DYP+ PK++F T+I+HPN+ G +C+ IL
Sbjct: 38 WEVMIIGPEDTLYEGGFFHATLSFPQDYPLMPPKMKFTTEIWHPNVHPNGEVCISILHPP 97
Query: 259 -----------DKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAELWKVNESEAIRNAK 405
++W P T+L+S+ ++LS+PN + P D A+ ++ N E + +
Sbjct: 98 GDDKYGYEDAGERWLPVHSPETILISVISMLSSPNDESPANIDAAKEFRENPQEFKKRVR 157
Query: 406 EWTRR 420
RR
Sbjct: 158 RLVRR 162
>SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme
Hus5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 157
Score = 64.9 bits (151), Expect = 1e-11
Identities = 31/90 (34%), Positives = 48/90 (53%), Gaps = 2/90 (2%)
Frame = +1
Query: 163 DYPMSAPKVRFITKIYHPNIDRLGRICLDILKDK--WSPALQIRTVLLSIQALLSAPNPD 336
+YP PK RF ++HPN+ G +CL IL ++ W PA+ I+ +LL IQ LL PN
Sbjct: 67 EYPTRPPKCRFTPPLFHPNVYPSGTVCLSILNEEEGWKPAITIKQILLGIQDLLDDPNIA 126
Query: 337 DPLANDVAELWKVNESEAIRNAKEWTRRYA 426
P + ++K ++ E + + R A
Sbjct: 127 SPAQTEAYTMFKKDKVEYEKRVRAQARENA 156
>SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme
Ubc3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 166
Score = 64.5 bits (150), Expect = 1e-11
Identities = 34/151 (22%), Positives = 69/151 (45%), Gaps = 14/151 (9%)
Frame = +1
Query: 10 KRHSD*CRSXVPGISAVPSETNARY-FHVIVTGPEDSXXXXXXXXXXXXXXXDYPMSAPK 186
K + + + GI+A PS + + + ++ GP+ + DYP+ P
Sbjct: 12 KEYKELTENGPDGITAGPSNEDDFFTWDCLIQGPDGTPFEGGLYPATLKFPSDYPLGPPT 71
Query: 187 VRFITKIYHPNIDRLGRICLDIL-------------KDKWSPALQIRTVLLSIQALLSAP 327
++F + +HPN+ + G +C+ IL ++WSP + +LLS+ ++L+ P
Sbjct: 72 LKFECEFFHPNVYKDGTVCISILHAPGDDPNMYESSSERWSPVQSVEKILLSVMSMLAEP 131
Query: 328 NPDDPLANDVAELWKVNESEAIRNAKEWTRR 420
N + D ++W+ + E R + R+
Sbjct: 132 NDESGANIDACKMWREDREEYCRVVRRLARK 162
>SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme
Ubc6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 227
Score = 44.4 bits (100), Expect = 1e-05
Identities = 25/101 (24%), Positives = 44/101 (43%), Gaps = 3/101 (2%)
Frame = +1
Query: 31 RSXVPGISAVPSETNARYFHVIVTGPEDSXXXXXXXXXXXXXXXDYPMSAPKVRFITKIY 210
++ V + A P+ N +H I+TGP D+ DYP P +R IT
Sbjct: 19 KNPVELVDAKPATENILEWHYIITGPPDTPYEGGQYHGTLIFPPDYPFKPPAIRMITP-- 76
Query: 211 HPNIDRLGRICL---DILKDKWSPALQIRTVLLSIQALLSA 324
R+CL D W+P+ + T+L+ + + +++
Sbjct: 77 SGRFQTNTRLCLSFSDFHPKSWNPSWMVSTILVGLVSFMTS 117
>SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme
Mms2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 139
Score = 27.9 bits (59), Expect = 1.4
Identities = 19/79 (24%), Positives = 35/79 (44%), Gaps = 4/79 (5%)
Frame = +1
Query: 85 FHVIVTGPEDSXXXXXXXXXXXXXXXDYPMSAPKVRFITKIYHPNID-RLGRI---CLDI 252
++ + GP S +YP + P V F+++I P +D G++ +D
Sbjct: 41 WNATILGPAHSVHENRIYSLKIHCDANYPDAPPIVTFVSRINLPGVDGETGKVNPHKIDC 100
Query: 253 LKDKWSPALQIRTVLLSIQ 309
L+ W + TVLL ++
Sbjct: 101 LR-HWKREYSMETVLLDLK 118
>SPCC1183.05c |lig4||DNA ligase Lig4|Schizosaccharomyces pombe|chr
3|||Manual
Length = 923
Score = 27.1 bits (57), Expect = 2.4
Identities = 9/26 (34%), Positives = 16/26 (61%)
Frame = +1
Query: 190 RFITKIYHPNIDRLGRICLDILKDKW 267
+FI ++HP+ RL R+C + + W
Sbjct: 219 KFILSVFHPDAARLYRLCSSLKRICW 244
>SPCC162.08c |nup211||nuclear pore complex associated
protein|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1837
Score = 25.8 bits (54), Expect = 5.5
Identities = 20/64 (31%), Positives = 33/64 (51%), Gaps = 2/64 (3%)
Frame = +1
Query: 223 DRLGRIC--LDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAELWKVNESEAIR 396
D LG +C LDI K K P LQ+ + L+S + + ND + +VN + +R
Sbjct: 10 DILG-VCSFLDIPKTKIDPLLQVDAFTSILIPLISKSKDYESIKND-RIVTEVNYEQQLR 67
Query: 397 NAKE 408
N+++
Sbjct: 68 NSEK 71
>SPCC757.11c |||membrane transporter|Schizosaccharomyces pombe|chr
3|||Manual
Length = 471
Score = 25.4 bits (53), Expect = 7.2
Identities = 8/26 (30%), Positives = 15/26 (57%)
Frame = +1
Query: 175 SAPKVRFITKIYHPNIDRLGRICLDI 252
++P V +TK+ HP +G + + I
Sbjct: 393 NSPSVSLLTKVLHPKYHLIGNVAVQI 418
>SPAC222.07c |hri2||eIF2 alpha kinase Hri2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 639
Score = 25.4 bits (53), Expect = 7.2
Identities = 14/39 (35%), Positives = 20/39 (51%)
Frame = -2
Query: 620 WITAKGPAPRPSSTNNKNLIHNSLSQVTLFRMCNITCTH 504
W+TAK P RPS N +QV++ + NI T+
Sbjct: 566 WMTAKDPTKRPSLLEVLNCGLLLPNQVSMPNISNIVSTN 604
>SPAC31G5.15 |||phosphatidylserine decarboxylase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 980
Score = 25.0 bits (52), Expect = 9.5
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = +1
Query: 238 ICLDILKDKWSPALQIRTVLLSIQALLSAPNPDD 339
+CL K KW + + + IQALL + NPD+
Sbjct: 111 LCL-YRKSKWKKQI-VGEAYIGIQALLLSTNPDE 142
>SPBC215.07c |||PWWP domain protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 568
Score = 25.0 bits (52), Expect = 9.5
Identities = 10/22 (45%), Positives = 14/22 (63%)
Frame = +1
Query: 217 NIDRLGRICLDILKDKWSPALQ 282
N D + IC DIL+++W LQ
Sbjct: 416 NEDEVKSICGDILENRWKSLLQ 437
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,585,386
Number of Sequences: 5004
Number of extensions: 49741
Number of successful extensions: 128
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 120
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 121
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 295793106
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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