Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fprWP07_F_K06
(666 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 209 3e-55
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 208 5e-55
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 117 2e-27
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 83 3e-17
SPCC777.11 |||sequence orphan|Schizosaccharomyces pombe|chr 3|||... 27 1.8
SPBC29A3.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 27 1.8
SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Sch... 26 4.2
SPCC965.07c |gst2||glutathione S-transferase Gst2|Schizosaccharo... 26 4.2
SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 7.4
SPAC23C4.12 |hhp2||serine/threonine protein kinase Hhp2 |Schizos... 25 9.8
SPAC2F7.16c |||phospholipase D |Schizosaccharomyces pombe|chr 1|... 25 9.8
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 209 bits (510), Expect = 3e-55
Identities = 92/140 (65%), Positives = 114/140 (81%)
Frame = +1
Query: 241 DDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYAR 420
D F+TFFSETG GK+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYAR
Sbjct: 46 DGGFSTFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYAR 105
Query: 421 GHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKS 600
GHYT+GKE+VD V D+IR++AD C+GLQGFL+FH LL+ERL+++Y KKS
Sbjct: 106 GHYTVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKS 165
Query: 601 KLEFAIYPAPQVSTXVRRAY 660
KL+F++YPAPQVST V Y
Sbjct: 166 KLQFSVYPAPQVSTSVVEPY 185
Score = 75.8 bits (178), Expect = 5e-15
Identities = 31/36 (86%), Positives = 33/36 (91%)
Frame = +3
Query: 105 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQM 212
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYM 36
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 208 bits (508), Expect = 5e-55
Identities = 89/140 (63%), Positives = 114/140 (81%)
Frame = +1
Query: 241 DDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYAR 420
+D F TFFSETG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYAR
Sbjct: 50 NDGFGTFFSETGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYAR 109
Query: 421 GHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKS 600
GHYT+GKE++D VL+RIR++AD C+GLQGFL+FH LL+ERL+++YGKKS
Sbjct: 110 GHYTVGKEMIDSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKS 169
Query: 601 KLEFAIYPAPQVSTXVRRAY 660
L+F++YPAPQVST V Y
Sbjct: 170 NLQFSVYPAPQVSTSVVEPY 189
Score = 75.8 bits (178), Expect = 5e-15
Identities = 34/38 (89%), Positives = 34/38 (89%)
Frame = +3
Query: 105 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPT 218
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPT 37
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 117 bits (281), Expect = 2e-27
Identities = 54/139 (38%), Positives = 80/139 (57%)
Frame = +1
Query: 244 DSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARG 423
+ N +F+E GK+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+G
Sbjct: 45 ERLNVYFNEAAGGKYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKG 104
Query: 424 HYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSK 603
HYT G E+ D VLD +R+ A+ C LQGF + H LL+ ++ +Y +
Sbjct: 105 HYTEGAELADAVLDVVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMM 164
Query: 604 LEFAIYPAPQVSTXVRRAY 660
F++ PAP+ S V Y
Sbjct: 165 ATFSVAPAPKSSDTVVEPY 183
Score = 34.7 bits (76), Expect = 0.012
Identities = 14/34 (41%), Positives = 18/34 (52%)
Frame = +3
Query: 105 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDG 206
MRE + + GQ G Q+G A W EHG+ G
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAG 34
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 83.0 bits (196), Expect = 3e-17
Identities = 47/144 (32%), Positives = 78/144 (54%), Gaps = 3/144 (2%)
Frame = +1
Query: 238 GDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANN 411
G D + FF ++ +++PRA+ +DLEP VV+ + + TY L++PE ++ K A NN
Sbjct: 44 GVDRKDVFFYQSDDTRYIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNN 103
Query: 412 YARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYG 591
+A G Y+ + I + ++D I + AD L+GF + H L+ERL+ Y
Sbjct: 104 WANG-YSHAERIFEDIMDMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYP 162
Query: 592 KKSKLEFAIYPAPQ-VSTXVRRAY 660
KK ++++P Q VS V + Y
Sbjct: 163 KKIIQTYSVFPNSQSVSDVVVQPY 186
Score = 46.8 bits (106), Expect = 3e-06
Identities = 19/35 (54%), Positives = 24/35 (68%)
Frame = +3
Query: 108 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQM 212
RE I++ GQ G QIG+ W+ CLEHGI PDG +
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTL 37
>SPCC777.11 |||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 128
Score = 27.5 bits (58), Expect = 1.8
Identities = 22/68 (32%), Positives = 37/68 (54%), Gaps = 4/68 (5%)
Frame = -2
Query: 281 PAPVSLKKVLKESSPPPMVL-DCGHLAIRLDAVLQAVKLPAG---ITDLDSGLANVYRDA 114
P+ VSLK++L+ + P P+ L + GHL +RL + +A I L+ G+ ++ A
Sbjct: 6 PSKVSLKRILQGNPPKPIGLTEYGHL-LRLVGLREADSKEENERTILKLNEGIIQMH--A 62
Query: 113 LTHFDLSF 90
+ D SF
Sbjct: 63 IERLDTSF 70
>SPBC29A3.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 398
Score = 27.5 bits (58), Expect = 1.8
Identities = 10/26 (38%), Positives = 19/26 (73%)
Frame = -2
Query: 275 PVSLKKVLKESSPPPMVLDCGHLAIR 198
PVS ++ +E+ PPM++ CGH+ ++
Sbjct: 342 PVSKEQATEEN--PPMMMSCGHVIVK 365
>SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1133
Score = 26.2 bits (55), Expect = 4.2
Identities = 12/30 (40%), Positives = 16/30 (53%)
Frame = +3
Query: 159 ACWELYCLEHGIQPDGQMPTVQDHRGWRRF 248
A + + C E G+Q DG P + H W RF
Sbjct: 452 ALYWMCCKEEGVQSDGSAPKL--HPLWSRF 479
>SPCC965.07c |gst2||glutathione S-transferase
Gst2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 230
Score = 26.2 bits (55), Expect = 4.2
Identities = 10/23 (43%), Positives = 16/23 (69%), Gaps = 1/23 (4%)
Frame = +3
Query: 168 ELYCLEH-GIQPDGQMPTVQDHR 233
E C EH + P+G++PT+ DH+
Sbjct: 40 EQKCKEHLALNPNGRVPTLVDHK 62
>SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 534
Score = 25.4 bits (53), Expect = 7.4
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = +1
Query: 94 LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 216
L+S +L+ P SR++ P S + STASSL P
Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210
>SPAC23C4.12 |hhp2||serine/threonine protein kinase Hhp2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 400
Score = 25.0 bits (52), Expect = 9.8
Identities = 10/23 (43%), Positives = 14/23 (60%)
Frame = -2
Query: 158 ITDLDSGLANVYRDALTHFDLSF 90
+T +D GLA YRD TH + +
Sbjct: 146 VTMIDFGLAKKYRDFKTHVHIPY 168
>SPAC2F7.16c |||phospholipase D |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1369
Score = 25.0 bits (52), Expect = 9.8
Identities = 7/29 (24%), Positives = 16/29 (55%)
Frame = +3
Query: 198 PDGQMPTVQDHRGWRRFFQHFLQRDRSWQ 284
PD +M T + + W+++ + F + W+
Sbjct: 1241 PDDEMLTWESYNEWKKYGKRFKEEQARWR 1269
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,826,651
Number of Sequences: 5004
Number of extensions: 60015
Number of successful extensions: 207
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 162
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 206
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 303841898
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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