BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fprWP07_F_I15
(653 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1442.10c |rpb3||DNA-directed RNA polymerase II subunit 3 |Sc... 27 1.8
SPAC17C9.06 |sam50||SAM complex subunit Sam50 |Schizosaccharomyc... 27 2.4
SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 27 3.1
SPCC777.13 |vps35||retromer complex subunit Vps35|Schizosaccharo... 26 4.1
SPAC23C4.16c |atg15||triacylglycerol lipase Atg15 |Schizosacchar... 26 5.5
SPCC338.17c |rad21||kleisin|Schizosaccharomyces pombe|chr 3|||Ma... 25 7.2
SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces ... 25 9.5
>SPCC1442.10c |rpb3||DNA-directed RNA polymerase II subunit 3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 297
Score = 27.5 bits (58), Expect = 1.8
Identities = 14/58 (24%), Positives = 26/58 (44%)
Frame = -2
Query: 430 PSIAGLKHRGPLKSVAKHHAQLGVRAHSLQVVLLEQGFWASSCSACCVKHTTNRLAHT 257
P +A K RGPL + ++ +R + + + E W+ + + N+L HT
Sbjct: 133 PILADPKSRGPLICKLRKEQEISLRCIAKKGIAKEHAKWSPTSAVAFEYDPWNKLQHT 190
>SPAC17C9.06 |sam50||SAM complex subunit Sam50 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 475
Score = 27.1 bits (57), Expect = 2.4
Identities = 16/46 (34%), Positives = 25/46 (54%)
Frame = +2
Query: 41 EYVVPSY*K*NAFDSLKKLIGFVYQXYLRTHLRLLSIKGEFHYQNI 178
EY PS + A DSLK+ + + Y R HL ++ KG++ Q +
Sbjct: 248 EYASPSV-RLEAGDSLKQSLSYTYTRDTRDHL-MIPTKGDYVRQTL 291
>SPAC29B12.07 |sec16||multidomain vesicle coat component
Sec16|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1995
Score = 26.6 bits (56), Expect = 3.1
Identities = 16/45 (35%), Positives = 22/45 (48%)
Frame = -3
Query: 639 DSRQHQLHQRHSRCQQMNFP*SSLTLRKLPSLLPFPCDPLLYSSL 505
DS + LH+R + + N P P LP P PLL+S+L
Sbjct: 1033 DSDKSSLHKRSAELSRNNSP--------RPDFLPLPNQPLLHSNL 1069
>SPCC777.13 |vps35||retromer complex subunit
Vps35|Schizosaccharomyces pombe|chr 3|||Manual
Length = 785
Score = 26.2 bits (55), Expect = 4.1
Identities = 17/48 (35%), Positives = 22/48 (45%), Gaps = 1/48 (2%)
Frame = +1
Query: 49 CAFLLK-IKCFR*LKKVNRVCLPXVFTNTFKTALYKRGISLSKYLGHY 189
C FL + + F+ + +V L F NT LYK G SL L Y
Sbjct: 512 CIFLARNFRIFKCMDWAEKVRLLWEFVNTCINVLYKNGDSLELCLALY 559
>SPAC23C4.16c |atg15||triacylglycerol lipase Atg15
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 424
Score = 25.8 bits (54), Expect = 5.5
Identities = 9/36 (25%), Positives = 17/36 (47%)
Frame = +3
Query: 288 TQHAEQLLAQNPCSSKTTCSECARTPSCAWCFATDF 395
T H Q + + + +C+ TP+C C+ +F
Sbjct: 384 THHRMQTVLNDVIDAYEKLPDCSHTPNCVDCYLWEF 419
>SPCC338.17c |rad21||kleisin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 628
Score = 25.4 bits (53), Expect = 7.2
Identities = 16/43 (37%), Positives = 22/43 (51%)
Frame = -1
Query: 488 DKQLIVGIENVGFIATTGTAFHCGIKTSRSIEVGGEAPRTAGS 360
D Q + I N G A T + H ++ SIEVG +AP A +
Sbjct: 199 DMQFHLPISNSG--AATPRSVHSDNQSQISIEVGRDAPAAAAT 239
>SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 3655
Score = 25.0 bits (52), Expect = 9.5
Identities = 14/42 (33%), Positives = 15/42 (35%)
Frame = -2
Query: 409 HRGPLKSVAKHHAQLGVRAHSLQVVLLEQGFWASSCSACCVK 284
H + HAQL V SL E GFW C K
Sbjct: 2564 HEWEKAQLVYEHAQLKVCTGSLPYSPTEHGFWLDHWILCAQK 2605
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,489,417
Number of Sequences: 5004
Number of extensions: 48973
Number of successful extensions: 115
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 112
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 115
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 295793106
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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