Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fprWP07_F_E01
(653 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosac... 91 1e-19
SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha... 87 2e-18
SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac... 77 3e-15
SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2... 76 5e-15
SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo... 73 4e-14
SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosacch... 73 5e-14
SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz... 69 6e-13
SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch... 66 4e-12
SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosacch... 62 7e-11
SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosac... 61 2e-10
SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 61 2e-10
SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac... 59 5e-10
SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc... 45 8e-06
SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizos... 33 0.027
SPCC622.07 |||dubious|Schizosaccharomyces pombe|chr 3|||Manual 28 1.4
SPBC28F2.07 |sfr1|dds20, mug13|Swi five-dependent recombination ... 27 1.8
SPCC4G3.18 |||conserved fungal family|Schizosaccharomyces pombe|... 27 3.1
SPAC22E12.16c |pik1||phosphatidylinositol kinase Pik1|Schizosacc... 27 3.1
SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gc... 26 4.1
SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual 26 5.5
SPCC1919.05 |||TPR repeat protein Ski3 |Schizosaccharomyces pomb... 25 7.2
>SPAC1250.03 |ubc14||ubiquitin conjugating enzyme
Ubc14|Schizosaccharomyces pombe|chr 1|||Manual
Length = 155
Score = 91.5 bits (217), Expect = 1e-19
Identities = 42/114 (36%), Positives = 67/114 (58%), Gaps = 1/114 (0%)
Frame = +2
Query: 224 AATRRLQKELNDIRQSGLKSFRDIQVDESNILTWQGLIV-PDNPPYNKGAFRIEINFPAE 400
+++RRL KE +D+R+ + R VD+ N+ W + P + Y G F + FP +
Sbjct: 7 SSSRRLTKEYSDLREHPIPDIRVNLVDD-NLFHWACTALGPSDSVYAGGKFHFSLKFPLD 65
Query: 401 YPFKPPKISFKTKIYHPNIDEKGQVCLPIISAENXKPATKTDQVIQALVALVND 562
YPF+PP I F T+IYHPN D +G VCL I+ + KP+ K V++ ++ L+ +
Sbjct: 66 YPFQPPTIEFTTRIYHPNFDSEGNVCLAILKQQVFKPSIKLRSVLEQILQLLRE 119
>SPBC119.02 |ubc4||ubiquitin conjugating enzyme
Ubc4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 147
Score = 87.4 bits (207), Expect = 2e-18
Identities = 42/113 (37%), Positives = 68/113 (60%), Gaps = 1/113 (0%)
Frame = +2
Query: 227 ATRRLQKELNDIRQSGLKSFRDIQVDESNILTWQGLIV-PDNPPYNKGAFRIEINFPAEY 403
A +R+ +EL D+ + S V + ++ WQ I+ P + PY G F + I+FP +Y
Sbjct: 2 ALKRINRELADLGKDPPSSCSAGPVGD-DLFHWQATIMGPADSPYAGGVFFLSIHFPTDY 60
Query: 404 PFKPPKISFKTKIYHPNIDEKGQVCLPIISAENXKPATKTDQVIQALVALVND 562
PFKPPK++F T+IYHPNI+ G +CL I+ + PA +V+ ++ +L+ D
Sbjct: 61 PFKPPKVNFTTRIYHPNINSNGSICLDIL-RDQWSPALTISKVLLSICSLLTD 112
>SPBC1105.09 |ubc15||ubiquitin conjugating enzyme
Ubc15|Schizosaccharomyces pombe|chr 2|||Manual
Length = 167
Score = 76.6 bits (180), Expect = 3e-15
Identities = 32/90 (35%), Positives = 58/90 (64%), Gaps = 1/90 (1%)
Frame = +2
Query: 224 AATRRLQKELNDIRQSGLKSFRDIQVDESNILTWQGLIV-PDNPPYNKGAFRIEINFPAE 400
A+ + L+K+L +I+++ + F VD+ +I W+ +I+ P++ Y G F ++FP +
Sbjct: 5 ASEQLLRKQLKEIQKNPPQGFSVGLVDDKSIFEWEVMIIGPEDTLYEGGFFHATLSFPQD 64
Query: 401 YPFKPPKISFKTKIYHPNIDEKGQVCLPII 490
YP PPK+ F T+I+HPN+ G+VC+ I+
Sbjct: 65 YPLMPPKMKFTTEIWHPNVHPNGEVCISIL 94
>SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2-C
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 176
Score = 75.8 bits (178), Expect = 5e-15
Identities = 33/90 (36%), Positives = 51/90 (56%), Gaps = 1/90 (1%)
Frame = +2
Query: 224 AATRRLQKELNDIRQSGLKSFRDIQVDESNILTWQGLIVPDNPPYNKGA-FRIEINFPAE 400
+ T+RL+ EL + S +SN+L W G I + Y +G F+I ++FPA
Sbjct: 28 SVTKRLRSELMSLMMSNTPGISAFPDSDSNLLHWAGTITGPSDTYYEGLKFKISMSFPAN 87
Query: 401 YPFKPPKISFKTKIYHPNIDEKGQVCLPII 490
YP+ PP I F + ++HPN+D G +CL I+
Sbjct: 88 YPYSPPTIIFTSPMWHPNVDMSGNICLDIL 117
>SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme
Ubc3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 166
Score = 72.9 bits (171), Expect = 4e-14
Identities = 32/96 (33%), Positives = 51/96 (53%), Gaps = 1/96 (1%)
Frame = +2
Query: 227 ATRRLQKELNDIRQSGLKSFRDIQVDESNILTWQGLIV-PDNPPYNKGAFRIEINFPAEY 403
A RRL KE ++ ++G +E + TW LI PD P+ G + + FP++Y
Sbjct: 6 ALRRLMKEYKELTENGPDGITAGPSNEDDFFTWDCLIQGPDGTPFEGGLYPATLKFPSDY 65
Query: 404 PFKPPKISFKTKIYHPNIDEKGQVCLPIISAENXKP 511
P PP + F+ + +HPN+ + G VC+ I+ A P
Sbjct: 66 PLGPPTLKFECEFFHPNVYKDGTVCISILHAPGDDP 101
>SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme
Ubc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 217
Score = 72.5 bits (170), Expect = 5e-14
Identities = 34/89 (38%), Positives = 56/89 (62%), Gaps = 2/89 (2%)
Frame = +2
Query: 230 TRRLQKELNDIRQSGLKSFRDIQVDESNILTWQGLIV-PDNPPYNKGAFRIEINFPAEYP 406
+RR+ KEL D++Q + +++ +I +G+ P+ PY G F ++I P +YP
Sbjct: 6 SRRIAKELADVQQDKQAGIQVWTIND-DISHLKGMFRGPEGTPYEGGYFVVDIEIPIDYP 64
Query: 407 FKPPKISFKTKIYHPNI-DEKGQVCLPII 490
F+PPK++F TKIYHPN+ + G +CL I+
Sbjct: 65 FRPPKMNFDTKIYHPNVSSQTGAICLDIL 93
>SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme
E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 151
Score = 68.9 bits (161), Expect = 6e-13
Identities = 31/113 (27%), Positives = 61/113 (53%), Gaps = 1/113 (0%)
Frame = +2
Query: 227 ATRRLQKELNDIRQSGLKSFRDIQVDESNILTWQGLIV-PDNPPYNKGAFRIEINFPAEY 403
A RRL ++ ++Q V + N++ W +I+ P + P+ G F++ ++F +Y
Sbjct: 5 ARRRLMRDFKRMQQDPPAGVSASPVSD-NVMLWNAVIIGPADTPFEDGTFKLVLSFDEQY 63
Query: 404 PFKPPKISFKTKIYHPNIDEKGQVCLPIISAENXKPATKTDQVIQALVALVND 562
P KPP + F + ++HPN+ G++CL I+ P ++ ++ +L+ND
Sbjct: 64 PNKPPLVKFVSTMFHPNVYANGELCLDILQ-NRWSPTYDVAAILTSIQSLLND 115
>SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme
Ubc13|Schizosaccharomyces pombe|chr 1|||Manual
Length = 148
Score = 66.1 bits (154), Expect = 4e-12
Identities = 28/72 (38%), Positives = 43/72 (59%)
Frame = +2
Query: 341 PDNPPYNKGAFRIEINFPAEYPFKPPKISFKTKIYHPNIDEKGQVCLPIISAENXKPATK 520
P Y G F +E+ P EYP PP + F TKIYHPN+D+ G++CL + ++ PA +
Sbjct: 41 PQQSAYEGGKFHLELFLPDEYPMMPPNVRFLTKIYHPNVDKLGRICLSTLK-KDWSPALQ 99
Query: 521 TDQVIQALVALV 556
V+ ++ AL+
Sbjct: 100 IRTVLLSIQALM 111
>SPBC211.07c |ubc8||ubiquitin conjugating enzyme
Ubc8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 62.1 bits (144), Expect = 7e-11
Identities = 24/52 (46%), Positives = 35/52 (67%), Gaps = 1/52 (1%)
Frame = +2
Query: 341 PDNPPYNKGAFRIEINFPAEYPFKPPKISFKTKIYHPNIDE-KGQVCLPIIS 493
P PY+ G +++ + P+EYP+K P I F +I+HPNIDE G VCL +I+
Sbjct: 39 PSETPYSGGIWKVHVELPSEYPWKSPSIGFVNRIFHPNIDELSGSVCLDVIN 90
>SPCC777.10c |ubc12||ubiquitin conjugating enzyme
Ubc12|Schizosaccharomyces pombe|chr 3|||Manual
Length = 177
Score = 60.9 bits (141), Expect = 2e-10
Identities = 34/103 (33%), Positives = 50/103 (48%)
Frame = +2
Query: 236 RLQKELNDIRQSGLKSFRDIQVDESNILTWQGLIVPDNPPYNKGAFRIEINFPAEYPFKP 415
R+QK++ D+ S + N+L + I PD Y G F+ I YP P
Sbjct: 27 RIQKDVTDLEIPSTMSTSWPDPIKLNVLHLE--IRPDEGYYKGGKFKFRIQIDDNYPHDP 84
Query: 416 PKISFKTKIYHPNIDEKGQVCLPIISAENXKPATKTDQVIQAL 544
PK+ KIYHPNID +G VCL I+ ++ P + ++ L
Sbjct: 85 PKVKCLNKIYHPNIDIEGNVCLNIL-RQDWNPVLNLNSILVGL 126
>SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme
Hus5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 157
Score = 60.9 bits (141), Expect = 2e-10
Identities = 28/87 (32%), Positives = 50/87 (57%), Gaps = 3/87 (3%)
Frame = +2
Query: 311 NILTWQGLIVPDNPP--YNKGAFRIEINFPAEYPFKPPKISFKTKIYHPNIDEKGQVCLP 484
+++ W+ + +P P + G +++ + FP EYP +PPK F ++HPN+ G VCL
Sbjct: 37 DLMNWK-VGIPGKPKTSWEGGLYKLTMAFPEEYPTRPPKCRFTPPLFHPNVYPSGTVCLS 95
Query: 485 IIS-AENXKPATKTDQVIQALVALVND 562
I++ E KPA Q++ + L++D
Sbjct: 96 ILNEEEGWKPAITIKQILLGIQDLLDD 122
>SPBC1198.09 |ubc16||ubiquitin conjugating enzyme
Ubc16|Schizosaccharomyces pombe|chr 2|||Manual
Length = 160
Score = 59.3 bits (137), Expect = 5e-10
Identities = 32/116 (27%), Positives = 60/116 (51%), Gaps = 3/116 (2%)
Frame = +2
Query: 224 AATRRLQKELNDIRQSGLKSFRDIQ-VDESNILTWQGLIV-PDNPPYNKGAFRIEINFPA 397
++ +RL KE + + ++ V + ++ W+ +I P PY G + ++I+
Sbjct: 3 SSIKRLHKEYASFQAGDISGLLLLKPVTDVDMFHWKAVIEGPTETPYEGGQWVLDIHVHE 62
Query: 398 EYPFKPPKISFKTKIYHPNID-EKGQVCLPIISAENXKPATKTDQVIQALVALVND 562
YP PP + F+TKI HPNI G+VC+ I+ + PA A+++L+++
Sbjct: 63 GYPISPPSVYFQTKIVHPNISWTNGEVCMDILKT-HWSPAWSLQSACLAIISLLSN 117
>SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme
Ubc6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 227
Score = 45.2 bits (102), Expect = 8e-06
Identities = 25/71 (35%), Positives = 38/71 (53%), Gaps = 1/71 (1%)
Frame = +2
Query: 227 ATRRLQKELNDIRQSGLKSFRDIQVDESNILTWQGLIV-PDNPPYNKGAFRIEINFPAEY 403
A +RL KE ++++ ++ D + NIL W +I P + PY G + + FP +Y
Sbjct: 6 AYKRLMKEYLALQKNPVELV-DAKPATENILEWHYIITGPPDTPYEGGQYHGTLIFPPDY 64
Query: 404 PFKPPKISFKT 436
PFKPP I T
Sbjct: 65 PFKPPAIRMIT 75
>SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme
Mms2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 139
Score = 33.5 bits (73), Expect = 0.027
Identities = 17/60 (28%), Positives = 32/60 (53%), Gaps = 2/60 (3%)
Frame = +2
Query: 302 DESNILTWQGLIV-PDNPPYNKGAFRIEINFPAEYPFKPPKISFKTKIYHPNID-EKGQV 475
D+ + W I+ P + + + ++I+ A YP PP ++F ++I P +D E G+V
Sbjct: 34 DDITLSDWNATILGPAHSVHENRIYSLKIHCDANYPDAPPIVTFVSRINLPGVDGETGKV 93
>SPCC622.07 |||dubious|Schizosaccharomyces pombe|chr 3|||Manual
Length = 128
Score = 27.9 bits (59), Expect = 1.4
Identities = 11/25 (44%), Positives = 16/25 (64%)
Frame = -2
Query: 466 LFINVRVVNLCFETNFRGFEWVFCW 392
LF+N V +C+ N+ GF + FCW
Sbjct: 41 LFLNYVVDIVCYVKNYNGFSF-FCW 64
>SPBC28F2.07 |sfr1|dds20, mug13|Swi five-dependent recombination
repair protein Sfr1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 299
Score = 27.5 bits (58), Expect = 1.8
Identities = 25/105 (23%), Positives = 45/105 (42%), Gaps = 3/105 (2%)
Frame = +2
Query: 230 TRRLQKELNDIRQSGLKSFRDIQVDESNILTWQGLIVPDNPP--YNKGAFRIEINFPAEY 403
++ + ELN+ S K + + ES++ QG + +NPP N G + F +
Sbjct: 2 SQTINSELNENATSQCKEDLKVSLSESDLRDSQGQLGIENPPKCNNSGNHSDNLGFIEQS 61
Query: 404 PFKPPKISFKTKIYHPNI-DEKGQVCLPIISAENXKPATKTDQVI 535
P+ K P++ D K LPI + + K A + ++
Sbjct: 62 ETVHPE---NEKALTPDLRDTKIHTSLPITTPFSKKRAREAKNIL 103
>SPCC4G3.18 |||conserved fungal family|Schizosaccharomyces pombe|chr
3|||Manual
Length = 828
Score = 26.6 bits (56), Expect = 3.1
Identities = 18/68 (26%), Positives = 29/68 (42%)
Frame = +2
Query: 308 SNILTWQGLIVPDNPPYNKGAFRIEINFPAEYPFKPPKISFKTKIYHPNIDEKGQVCLPI 487
S++ T + LI P PP K E E F+P + ++ I P +D + +
Sbjct: 566 SSMQTIESLIHPRFPPLQKHLSPSEFENTFESRFEPVSLENQSHISPPQVDSQSEYKESS 625
Query: 488 ISAENXKP 511
I A + P
Sbjct: 626 ILAPSNPP 633
>SPAC22E12.16c |pik1||phosphatidylinositol kinase
Pik1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 851
Score = 26.6 bits (56), Expect = 3.1
Identities = 20/73 (27%), Positives = 34/73 (46%), Gaps = 6/73 (8%)
Frame = -2
Query: 388 VNLNAESTFVVRWVVWNN*TLPC*DVG-LINLYVSERLQARL-----SDVIKFFL*SSRC 227
V NA+ ++ V+ NN T+PC G + + +R Q +L D + + + C
Sbjct: 774 VRKNADQIILLVEVMQNNSTMPCFRAGENTSAQLLQRFQLQLGDKECDDFVDLLIQKANC 833
Query: 226 SHCTKFAKMSQNI 188
S T+ + QNI
Sbjct: 834 SVWTRLYDLFQNI 846
>SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gcn2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1576
Score = 26.2 bits (55), Expect = 4.1
Identities = 15/41 (36%), Positives = 22/41 (53%), Gaps = 2/41 (4%)
Frame = -3
Query: 441 IFVLKLIFGG--LNGYSAGKLISMRKAPLL*GGLSGTIRPC 325
+ VL+++ G LN +S+ KLI PLL G +R C
Sbjct: 450 LLVLQMLCGAHVLNKFSSLKLIMTHVIPLLPGSYQDLVRRC 490
>SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual
Length = 4717
Score = 25.8 bits (54), Expect = 5.5
Identities = 17/55 (30%), Positives = 24/55 (43%)
Frame = +2
Query: 182 LWNILGHFSKFCTMAATRRLQKELNDIRQSGLKSFRDIQVDESNILTWQGLIVPD 346
LWN+L FSKF A+ + + ++ F + V NI W VPD
Sbjct: 2313 LWNLLNQFSKFLIEIASAN-SNIVYKLSYDVIRHFLKLVVLWKNIYVWTN--VPD 2364
>SPCC1919.05 |||TPR repeat protein Ski3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1389
Score = 25.4 bits (53), Expect = 7.2
Identities = 11/30 (36%), Positives = 21/30 (70%)
Frame = +2
Query: 245 KELNDIRQSGLKSFRDIQVDESNILTWQGL 334
K+L++ +++ L + I++DE +L WQGL
Sbjct: 50 KQLSESKEAYLDA---IKIDEKAVLAWQGL 76
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,539,480
Number of Sequences: 5004
Number of extensions: 52671
Number of successful extensions: 136
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 128
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 132
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 295793106
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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