Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fprWP06_F_F20
(543 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1450.04 |tef5||translation elongation factor EF-1 beta subun... 87 1e-18
SPBC887.02 |||ClC chloride channel|Schizosaccharomyces pombe|chr... 27 1.8
SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomy... 27 2.4
SPBC342.02 |||glutaminyl-tRNA synthetase |Schizosaccharomyces po... 25 5.5
SPBC23G7.08c |rga7||GTPase activating protein Rga7|Schizosacchar... 25 5.5
SPAC1527.03 |||RNA-binding protein|Schizosaccharomyces pombe|chr... 25 5.5
SPBC646.14c |orc5||origin recognition complex subunit Orc5|Schiz... 25 7.2
SPBC146.01 |med15|SPBP35G2.15|mediator complex subunit Med15 |Sc... 25 9.5
SPAC23G3.01 |rpb2|SPAC521.06|DNA-directed RNA polymerase II comp... 25 9.5
>SPCC1450.04 |tef5||translation elongation factor EF-1 beta subunit
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 214
Score = 87.4 bits (207), Expect = 1e-18
Identities = 55/146 (37%), Positives = 68/146 (46%), Gaps = 1/146 (0%)
Frame = +1
Query: 103 LNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAP-AANLPHVLRWYNQIASYTPAERKTW 279
L LN +L +KS++ GY PSQAD VF+ VG AP A P+ RWY QIA+Y A
Sbjct: 12 LKQLNDFLLDKSFIEGYEPSQADAVVFKAVGVAPDTAKYPNGARWYKQIATYDLA----- 66
Query: 280 SQGTSPLXXXXXXXXXXXXXXXXXXXXVDLFGSGXXXXXXXXXXXXXXXXKAYADKKSKK 459
T P +DLFGS + Y KK+ K
Sbjct: 67 ---TLPGTAKEVSAYGPEGAAAAEEDEIDLFGSDEEEDPEAERIKAERVAE-YNKKKAAK 122
Query: 460 PXLIAKSSILLDVXPWDDETDMKEME 537
P + KS + LDV PWDDET M E+E
Sbjct: 123 PKAVHKSLVTLDVKPWDDETPMDELE 148
>SPBC887.02 |||ClC chloride channel|Schizosaccharomyces pombe|chr
2|||Manual
Length = 667
Score = 27.1 bits (57), Expect = 1.8
Identities = 13/37 (35%), Positives = 20/37 (54%)
Frame = -1
Query: 291 GALRPSFAFSRSV*SNLIIPS*YVGKVSGRRLANLLK 181
G L S F ++ + +I+PS +G GR + LLK
Sbjct: 394 GLLLTSATFGAAIPTGIIVPSLAIGACIGRAVGTLLK 430
>SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 4924
Score = 26.6 bits (56), Expect = 2.4
Identities = 15/53 (28%), Positives = 23/53 (43%)
Frame = +1
Query: 103 LNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQIASYTP 261
L+ + YL++ Y T Q + Q+F PA L W N + +Y P
Sbjct: 4648 LDIMINYLSKMYYDDSLTIVQQNSQLFLSTVLDPAVGLSRKKFWNNYLTNYKP 4700
>SPBC342.02 |||glutaminyl-tRNA synthetase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 811
Score = 25.4 bits (53), Expect = 5.5
Identities = 11/32 (34%), Positives = 19/32 (59%)
Frame = +1
Query: 442 DKKSKKPXLIAKSSILLDVXPWDDETDMKEME 537
DK+S P LIA++ + ++ D+ +KE E
Sbjct: 692 DKESNSPVLIAETRLFNNLFKCDNPAALKEQE 723
>SPBC23G7.08c |rga7||GTPase activating protein
Rga7|Schizosaccharomyces pombe|chr 2|||Manual
Length = 695
Score = 25.4 bits (53), Expect = 5.5
Identities = 11/19 (57%), Positives = 12/19 (63%)
Frame = +2
Query: 302 PPVLNPRLPPQQRKTTMTT 358
PPVL P LPP Q T T+
Sbjct: 449 PPVLLPTLPPIQTTTIQTS 467
>SPAC1527.03 |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 475
Score = 25.4 bits (53), Expect = 5.5
Identities = 21/66 (31%), Positives = 28/66 (42%), Gaps = 4/66 (6%)
Frame = -3
Query: 241 DYTIVVRGEG*RPAPCQLAQILEHQLEK----ECIQTRSFSQLNIGSSH*EPWAVLTSPT 74
+Y +VV G G P C + L QLE E + F + ++ P A L S
Sbjct: 308 NYPVVVNGNGVNPYLCDVQAFLTSQLEYYFSIENLCKDMFLRKHMDDEGYVPLAFLASFN 367
Query: 73 AIVSFS 56
I SFS
Sbjct: 368 RIKSFS 373
>SPBC646.14c |orc5||origin recognition complex subunit
Orc5|Schizosaccharomyces pombe|chr 2|||Manual
Length = 455
Score = 25.0 bits (52), Expect = 7.2
Identities = 9/27 (33%), Positives = 18/27 (66%)
Frame = +1
Query: 103 LNDLNQYLAEKSYVSGYTPSQADVQVF 183
L+ +++YL ++++ Y PS+ D Q F
Sbjct: 300 LSLVSKYLLVSAFLASYNPSRLDAQFF 326
>SPBC146.01 |med15|SPBP35G2.15|mediator complex subunit Med15
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1063
Score = 24.6 bits (51), Expect = 9.5
Identities = 11/33 (33%), Positives = 19/33 (57%)
Frame = +2
Query: 320 RLPPQQRKTTMTTTLIYLVLVTRKRMQKLNEFE 418
RLPP+Q K +L ++ + R Q+LN+ +
Sbjct: 384 RLPPEQLKLIGMLYRKHLQIILQHRQQQLNKIQ 416
>SPAC23G3.01 |rpb2|SPAC521.06|DNA-directed RNA polymerase II complex
subunit Rpb2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1210
Score = 24.6 bits (51), Expect = 9.5
Identities = 11/26 (42%), Positives = 14/26 (53%)
Frame = +2
Query: 29 CGYLEKSCYTKRNYGCRRR*NRPGFS 106
CG + + Y K +Y CR NR FS
Sbjct: 1155 CGLIAIASYKKDSYECRSCQNRTRFS 1180
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.313 0.128 0.393
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,851,202
Number of Sequences: 5004
Number of extensions: 31276
Number of successful extensions: 54
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 52
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 53
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 223909422
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.2 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.9 bits)
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