Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fprWP05_F_M02
(348 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 185 2e-48
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 185 2e-48
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 185 2e-48
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 96 2e-21
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 95 2e-21
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 52 3e-08
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 44 5e-06
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 36 0.001
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 36 0.002
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 36 0.002
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 35 0.003
SPAPYUG7.03c |mid2||anillin homologue Mid2|Schizosaccharomyces p... 30 0.12
SPCC63.03 |||DNAJ domain protein, DNAJC11 family|Schizosaccharom... 27 0.63
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 25 3.4
SPBC1703.13c |||inorganic phosphate transporter |Schizosaccharom... 25 3.4
SPAC2G11.11c |prh1||ATP-dependent RNA helicase Prh1|Schizosaccha... 24 5.9
SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal |Schizos... 24 5.9
SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit... 24 7.8
SPBC1711.17 |prp16|SPBC17G9.01|ATP-dependent RNA helicase Prp16|... 24 7.8
SPCC364.02c |bis1||stress response protein Bis1|Schizosaccharomy... 24 7.8
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 24 7.8
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 185 bits (451), Expect = 2e-48
Identities = 85/94 (90%), Positives = 88/94 (93%)
Frame = +1
Query: 64 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIXKFXKEAQEMGKGSFKYAWVL 243
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTI KF KEA E+GKGSFKYAWVL
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 244 DKLKAERERGITIDIALWKFETSKYYVTIIDAPG 345
DKLKAERERGITIDIALWKFET KY VT+IDAPG
Sbjct: 61 DKLKAERERGITIDIALWKFETPKYNVTVIDAPG 94
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 185 bits (451), Expect = 2e-48
Identities = 85/94 (90%), Positives = 88/94 (93%)
Frame = +1
Query: 64 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIXKFXKEAQEMGKGSFKYAWVL 243
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTI KF KEA E+GKGSFKYAWVL
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 244 DKLKAERERGITIDIALWKFETSKYYVTIIDAPG 345
DKLKAERERGITIDIALWKFET KY VT+IDAPG
Sbjct: 61 DKLKAERERGITIDIALWKFETPKYNVTVIDAPG 94
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 185 bits (451), Expect = 2e-48
Identities = 85/94 (90%), Positives = 88/94 (93%)
Frame = +1
Query: 64 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIXKFXKEAQEMGKGSFKYAWVL 243
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTI KF KEA E+GKGSFKYAWVL
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 244 DKLKAERERGITIDIALWKFETSKYYVTIIDAPG 345
DKLKAERERGITIDIALWKFET KY VT+IDAPG
Sbjct: 61 DKLKAERERGITIDIALWKFETPKYNVTVIDAPG 94
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 95.9 bits (228), Expect = 2e-21
Identities = 44/90 (48%), Positives = 60/90 (66%)
Frame = +1
Query: 76 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIXKFXKEAQEMGKGSFKYAWVLDKLK 255
K +++VV GHVDSGKST G ++++ G I+ R++ K EA GKGSF YAW+LD +
Sbjct: 175 KPVVHLVVTGHVDSGKSTMLGRIMFELGEINSRSMQKLHNEAANSGKGSFSYAWLLDTTE 234
Query: 256 AERERGITIDIALWKFETSKYYVTIIDAPG 345
ER RG+T+D+A FE+ K I DAPG
Sbjct: 235 EERARGVTMDVASTTFESDKKIYEIGDAPG 264
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 95.5 bits (227), Expect = 2e-21
Identities = 42/90 (46%), Positives = 61/90 (67%)
Frame = +1
Query: 76 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIXKFXKEAQEMGKGSFKYAWVLDKLK 255
K H+NIV IGHVD+GKST G++++ G +DKRT+ K +EA+E GK S+ +W LD
Sbjct: 236 KEHVNIVFIGHVDAGKSTLGGNILFLTGMVDKRTMEKIEREAKEAGKESWYLSWALDSTS 295
Query: 256 AERERGITIDIALWKFETSKYYVTIIDAPG 345
ERE+G T+++ FET +++DAPG
Sbjct: 296 EEREKGKTVEVGRAYFETEHRRFSLLDAPG 325
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 51.6 bits (118), Expect = 3e-08
Identities = 33/92 (35%), Positives = 47/92 (51%)
Frame = +1
Query: 70 KEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIXKFXKEAQEMGKGSFKYAWVLDK 249
++K H+NI IGHVD GK+T T I KC ++G+ SF +DK
Sbjct: 49 RKKPHVNIGTIGHVDHGKTTLTA-AITKC--------------LSDLGQASFMDYSQIDK 93
Query: 250 LKAERERGITIDIALWKFETSKYYVTIIDAPG 345
E+ RGITI A ++ET+ + +D PG
Sbjct: 94 APEEKARGITISSAHVEYETANRHYAHVDCPG 125
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 44.4 bits (100), Expect = 5e-06
Identities = 29/86 (33%), Positives = 42/86 (48%)
Frame = +1
Query: 88 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIXKFXKEAQEMGKGSFKYAWVLDKLKAERE 267
N+ +I H+D+GK+T T ++Y G F + G V+D L AER+
Sbjct: 30 NVGIIAHIDAGKTTLTEKMLYYGG---------FTSHFGNVDTGDT----VMDYLPAERQ 76
Query: 268 RGITIDIALWKFETSKYYVTIIDAPG 345
RGITI+ A F + +ID PG
Sbjct: 77 RGITINSAAISFTWRNQRINLIDTPG 102
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 36.3 bits (80), Expect = 0.001
Identities = 24/68 (35%), Positives = 36/68 (52%)
Frame = +1
Query: 88 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIXKFXKEAQEMGKGSFKYAWVLDKLKAERE 267
NI + H+DSGK+T T ++Y G I K+ E+ +G +D ++ ERE
Sbjct: 61 NIGISAHIDSGKTTFTERVLYYTGRI---------KDIHEV-RGKDNVGAKMDFMELERE 110
Query: 268 RGITIDIA 291
+GITI A
Sbjct: 111 KGITIQSA 118
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 35.9 bits (79), Expect = 0.002
Identities = 28/73 (38%), Positives = 34/73 (46%)
Frame = +1
Query: 64 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIXKFXKEAQEMGKGSFKYAWVL 243
MGK N+ VI HVD GKST T L+ K G I A + G F +
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGII----------SAAKAGDARF-----M 57
Query: 244 DKLKAERERGITI 282
D E+ERG+TI
Sbjct: 58 DTRADEQERGVTI 70
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 35.9 bits (79), Expect = 0.002
Identities = 28/73 (38%), Positives = 34/73 (46%)
Frame = +1
Query: 64 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIXKFXKEAQEMGKGSFKYAWVL 243
MGK N+ VI HVD GKST T L+ K G I A + G F +
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGII----------SAAKAGDARF-----M 57
Query: 244 DKLKAERERGITI 282
D E+ERG+TI
Sbjct: 58 DTRADEQERGVTI 70
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 35.1 bits (77), Expect = 0.003
Identities = 27/90 (30%), Positives = 41/90 (45%), Gaps = 4/90 (4%)
Frame = +1
Query: 88 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIXKFXKEAQEMGKGSFKYAWVLDKLKAERE 267
N VI H+D GKST + ++ G I++ + F LDKL+ ER
Sbjct: 60 NWAVIAHIDHGKSTLSDCILKLTGVINEHNF-----------RNQF-----LDKLEVERR 103
Query: 268 RGITIDI----ALWKFETSKYYVTIIDAPG 345
RGIT+ ++ + Y + +ID PG
Sbjct: 104 RGITVKAQTCSMIYYYHGQSYLLNLIDTPG 133
>SPAPYUG7.03c |mid2||anillin homologue Mid2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 704
Score = 29.9 bits (64), Expect = 0.12
Identities = 14/43 (32%), Positives = 23/43 (53%)
Frame = -2
Query: 281 IVIPRSRSAFSLSNTQAYLKDPLPISWASXSNXSMVRLSIPPH 153
I IP+ A S+ N++ + +D LP + S V+L +P H
Sbjct: 171 IPIPKKNPARSVCNSKLFNEDTLPAEFEEVSISPPVKLELPTH 213
>SPCC63.03 |||DNAJ domain protein, DNAJC11
family|Schizosaccharomyces pombe|chr 3|||Manual
Length = 642
Score = 27.5 bits (58), Expect = 0.63
Identities = 16/44 (36%), Positives = 22/44 (50%)
Frame = -2
Query: 305 SNFQRAISIVIPRSRSAFSLSNTQAYLKDPLPISWASXSNXSMV 174
S F IS+ +P FSL+ ++ K LPI W S + S V
Sbjct: 403 STFGVNISVGVPTGSITFSLNWSRLGQKISLPIMWCSVFDRSAV 446
>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 25.0 bits (52), Expect = 3.4
Identities = 12/24 (50%), Positives = 14/24 (58%)
Frame = +1
Query: 58 PKMGKEKTHINIVVIGHVDSGKST 129
P + + INI IGHV GKST
Sbjct: 15 PAIISRQATINIGTIGHVAHGKST 38
>SPBC1703.13c |||inorganic phosphate transporter
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 311
Score = 25.0 bits (52), Expect = 3.4
Identities = 8/19 (42%), Positives = 12/19 (63%)
Frame = -3
Query: 205 PGPPXRTXRWYVCQYHHIC 149
P PP +T + Y QY+ +C
Sbjct: 8 PAPPKKTLQLYTPQYYGLC 26
>SPAC2G11.11c |prh1||ATP-dependent RNA helicase
Prh1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 719
Score = 24.2 bits (50), Expect = 5.9
Identities = 10/22 (45%), Positives = 13/22 (59%)
Frame = +1
Query: 91 IVVIGHVDSGKSTTTGHLIYKC 156
IVV+G SGKST + +C
Sbjct: 115 IVVVGETGSGKSTQIPQFLNEC 136
>SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 647
Score = 24.2 bits (50), Expect = 5.9
Identities = 8/22 (36%), Positives = 13/22 (59%)
Frame = +1
Query: 94 VVIGHVDSGKSTTTGHLIYKCG 159
+V+GH GK+T +Y+ G
Sbjct: 45 IVLGHTGCGKTTQIPQFLYEAG 66
>SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit
Gpa2 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 354
Score = 23.8 bits (49), Expect = 7.8
Identities = 13/41 (31%), Positives = 21/41 (51%), Gaps = 1/41 (2%)
Frame = +1
Query: 70 KEKTHINIVVIGHVDSGKSTTTGHL-IYKCGGIDKRTIXKF 189
K+K ++++G DSGKST + + I G + I F
Sbjct: 30 KDKKIYKVLLLGASDSGKSTISKQIKILNKNGFSQEEIMTF 70
>SPBC1711.17 |prp16|SPBC17G9.01|ATP-dependent RNA helicase
Prp16|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1173
Score = 23.8 bits (49), Expect = 7.8
Identities = 8/23 (34%), Positives = 14/23 (60%)
Frame = +1
Query: 91 IVVIGHVDSGKSTTTGHLIYKCG 159
++V+G SGK+T +Y+ G
Sbjct: 511 LIVVGETGSGKTTQLAQFLYEDG 533
>SPCC364.02c |bis1||stress response protein Bis1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 384
Score = 23.8 bits (49), Expect = 7.8
Identities = 18/60 (30%), Positives = 29/60 (48%)
Frame = -2
Query: 338 ASMMVT*YLLVSNFQRAISIVIPRSRSAFSLSNTQAYLKDPLPISWASXSNXSMVRLSIP 159
AS + Y ++ R ++ + P +R + S YL+ PL S S S + +R SIP
Sbjct: 309 ASTALNSYSTPNSVSRKLTNLTPAARRLVARS----YLRSPLHGSSPSASRHTALRTSIP 364
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 23.8 bits (49), Expect = 7.8
Identities = 10/29 (34%), Positives = 15/29 (51%)
Frame = +1
Query: 88 NIVVIGHVDSGKSTTTGHLIYKCGGIDKR 174
N ++ HVD GK+T L+ G I +
Sbjct: 21 NFTLLAHVDHGKTTLADSLLASNGIISSK 49
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,293,248
Number of Sequences: 5004
Number of extensions: 21537
Number of successful extensions: 75
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 71
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 74
length of database: 2,362,478
effective HSP length: 64
effective length of database: 2,042,222
effective search space used: 104153322
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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