BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fprWP05_F_H04
(502 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1250.05 |rpl3002|rpl30-2, rpl30|60S ribosomal protein L30|Sc... 77 2e-15
SPAC9G1.03c |rpl3001|rpl30-1, rpl30|60S ribosomal protein L30|Sc... 76 3e-15
SPAC14C4.13 |rad17||RFC related checkpoint protein Rad17|Schizos... 29 0.52
SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccha... 27 1.6
SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyce... 25 4.8
SPBC839.03c |||neddylation protein Dcn1|Schizosaccharomyces pomb... 25 6.4
>SPAC1250.05 |rpl3002|rpl30-2, rpl30|60S ribosomal protein
L30|Schizosaccharomyces pombe|chr 1|||Manual
Length = 117
Score = 76.6 bits (180), Expect = 2e-15
Identities = 32/47 (68%), Positives = 39/47 (82%)
Frame = +3
Query: 234 EIEYYALLAXTGVHHYSGNNIELGTACGKYYRVCTLAITDPGDSDII 374
E+EYYA+L+ VHHYSGNNI+LGTACGK +RV LA+ D GDSDI+
Sbjct: 69 ELEYYAMLSRCSVHHYSGNNIDLGTACGKLFRVGVLAVIDAGDSDIL 115
Score = 74.5 bits (175), Expect = 8e-15
Identities = 35/58 (60%), Positives = 41/58 (70%)
Frame = +2
Query: 47 TLPKWLQQRNRKRPSSQLNSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVIIAKNAPP 220
T P + K+ +NS+LAL MKSGKY LGYK TLKTLR GKAKL++IA NAPP
Sbjct: 7 TAPVAAVSKKGKKSGDTINSKLALTMKSGKYVLGYKSTLKTLRSGKAKLILIAANAPP 64
>SPAC9G1.03c |rpl3001|rpl30-1, rpl30|60S ribosomal protein
L30|Schizosaccharomyces pombe|chr 1|||Manual
Length = 109
Score = 76.2 bits (179), Expect = 3e-15
Identities = 32/47 (68%), Positives = 39/47 (82%)
Frame = +3
Query: 234 EIEYYALLAXTGVHHYSGNNIELGTACGKYYRVCTLAITDPGDSDII 374
E+EYYA+L+ VHHY+G NI+LGTACGK +RV LAITD GDSDI+
Sbjct: 61 ELEYYAMLSKANVHHYAGTNIDLGTACGKLFRVGVLAITDAGDSDIL 107
Score = 70.5 bits (165), Expect = 1e-13
Identities = 31/50 (62%), Positives = 38/50 (76%)
Frame = +2
Query: 71 RNRKRPSSQLNSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVIIAKNAPP 220
+ K+ +N++LAL MKSGKY LGYK TLKTLR GKAKL++IA N PP
Sbjct: 7 KKSKKSGDTINAKLALTMKSGKYVLGYKSTLKTLRSGKAKLILIAGNCPP 56
>SPAC14C4.13 |rad17||RFC related checkpoint protein
Rad17|Schizosaccharomyces pombe|chr 1|||Manual
Length = 606
Score = 28.7 bits (61), Expect = 0.52
Identities = 12/34 (35%), Positives = 20/34 (58%)
Frame = +2
Query: 38 LAFTLPKWLQQRNRKRPSSQLNSRLALVMKSGKY 139
L ++L +WL N K PS+Q + L+L K ++
Sbjct: 129 LGYSLIEWLNPMNLKEPSNQESDTLSLTEKFSRF 162
>SPAC1834.01 |sup45||translation release factor
eRF1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 433
Score = 27.1 bits (57), Expect = 1.6
Identities = 9/26 (34%), Positives = 16/26 (61%)
Frame = +2
Query: 122 MKSGKYCLGYKQTLKTLRQGKAKLVI 199
+ SGKYC G T+ L++G + ++
Sbjct: 293 LDSGKYCFGVVDTMNALQEGAVETLL 318
>SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 154
Score = 25.4 bits (53), Expect = 4.8
Identities = 15/46 (32%), Positives = 27/46 (58%), Gaps = 4/46 (8%)
Frame = +2
Query: 95 QLNSRLALVMKSG---KYCL-GYKQTLKTLRQGKAKLVIIAKNAPP 220
+LN ++ +K K+ L G K+ +K +R+G+ LVI+A + P
Sbjct: 36 KLNKKMMKTVKKASKQKHILRGVKEVVKAVRKGEKGLVILAGDISP 81
>SPBC839.03c |||neddylation protein Dcn1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 251
Score = 25.0 bits (52), Expect = 6.4
Identities = 8/17 (47%), Positives = 13/17 (76%)
Frame = +2
Query: 56 KWLQQRNRKRPSSQLNS 106
KW+QQ++R+ QLN+
Sbjct: 40 KWIQQKSREEAEKQLNN 56
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,660,193
Number of Sequences: 5004
Number of extensions: 30178
Number of successful extensions: 72
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 70
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 72
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 198176188
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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