Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fprWP03_F_K08
(621 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q9VBP9 Cluster: CG4673-PA, isoform A; n=8; Endopterygot... 77 5e-13
UniRef50_Q63ZR4 Cluster: LOC494816 protein; n=3; Xenopus|Rep: LO... 53 5e-06
UniRef50_UPI0000D9E56B Cluster: PREDICTED: similar to Nuclear pr... 50 3e-05
UniRef50_Q8TAT6 Cluster: Nuclear protein localization protein 4 ... 50 4e-05
UniRef50_Q6C619 Cluster: Yarrowia lipolytica chromosome E of str... 50 6e-05
UniRef50_Q9P780 Cluster: Cdc48-Ufd1-Npl4 complex component Npl4;... 45 0.001
UniRef50_P33755 Cluster: Protein NPL4; n=6; Saccharomycetales|Re... 43 0.005
UniRef50_Q95QZ9 Cluster: Putative uncharacterized protein; n=5; ... 43 0.007
UniRef50_A3GFS1 Cluster: Nuclear protein localization factor and... 43 0.007
UniRef50_A1CS06 Cluster: Endoplasmic reticulum and nuclear membr... 42 0.009
UniRef50_Q4PGP9 Cluster: Putative uncharacterized protein; n=1; ... 41 0.021
UniRef50_Q5KKN9 Cluster: ER-associated protein catabolism-relate... 41 0.027
UniRef50_A5DBC9 Cluster: Putative uncharacterized protein; n=1; ... 40 0.036
UniRef50_Q8SRJ9 Cluster: PROTEIN INVOLVED IN ER TRANSLOCATION; n... 40 0.063
UniRef50_Q4S8H6 Cluster: Chromosome 2 SCAF14705, whole genome sh... 38 0.15
UniRef50_A2FMY9 Cluster: Putative uncharacterized protein; n=1; ... 35 1.4
UniRef50_UPI0000D9E5FA Cluster: PREDICTED: similar to nuclear pr... 35 1.8
UniRef50_Q1NLM2 Cluster: Putative uncharacterized protein; n=1; ... 34 2.4
UniRef50_Q9W0J2 Cluster: CG13913-PA; n=3; Diptera|Rep: CG13913-P... 33 7.3
UniRef50_UPI0000F1FDE7 Cluster: PREDICTED: similar to BC055004 p... 32 9.6
UniRef50_A0XZT4 Cluster: Putative uncharacterized protein; n=1; ... 32 9.6
>UniRef50_Q9VBP9 Cluster: CG4673-PA, isoform A; n=8;
Endopterygota|Rep: CG4673-PA, isoform A - Drosophila
melanogaster (Fruit fly)
Length = 652
Score = 76.6 bits (180), Expect = 5e-13
Identities = 53/166 (31%), Positives = 75/166 (45%)
Frame = +1
Query: 19 QKMLLRVQSPEGTARVEVLDSDVTAHLFXRIYEALNLNSFAFTLHKDRQRKEEITSSKSR 198
++ L+RVQS EG R+E+ HL+ + AL ++ F L K+R E+ +S S
Sbjct: 32 KQSLIRVQSAEGIKRIEISPKSNLKHLYDSVQNALKVDGFG--LFKERNFLTELQASGS- 88
Query: 199 QLRDCGLCHGDMIYLNPVNGAVLFEQSSTSSETHRKXXXXXXXXXXXXXXXXXTSKVTLX 378
QL L HGDM+YL + G S+T ++ TS ++
Sbjct: 89 QLVGTSLRHGDMVYLKQMAGTSSRRTSTTVLDSQ----------------AFKTSTISNP 132
Query: 379 XXXXXXXXXXXXXVHLQLYKVSGLIERXRDEKLCRHNLNGKCVHCT 516
V L K G I+R RD KLC HN NG+CVHC+
Sbjct: 133 NSARPSFNVIEDDVDQALSKADGTIKRERDSKLCHHNANGRCVHCS 178
Score = 51.2 bits (117), Expect = 2e-05
Identities = 21/28 (75%), Positives = 24/28 (85%)
Frame = +3
Query: 519 LXPWDEXYLKEHNIKHMSFHSYLPKITS 602
L P+DE YLKEHNIKH+SFHSY+ K TS
Sbjct: 180 LEPYDESYLKEHNIKHLSFHSYIRKQTS 207
>UniRef50_Q63ZR4 Cluster: LOC494816 protein; n=3; Xenopus|Rep:
LOC494816 protein - Xenopus laevis (African clawed frog)
Length = 610
Score = 53.2 bits (122), Expect = 5e-06
Identities = 22/82 (26%), Positives = 46/82 (56%)
Frame = +1
Query: 16 SQKMLLRVQSPEGTARVEVLDSDVTAHLFXRIYEALNLNSFAFTLHKDRQRKEEITSSKS 195
S+ +++R+QSP+G R+ + ++ + + F+++ +R R EI++S++
Sbjct: 2 SESIVIRIQSPDGVKRINASKRETAVMFLKKVAKEFGFTNNRFSVYVNRNRTGEISASQN 61
Query: 196 RQLRDCGLCHGDMIYLNPVNGA 261
+ L + HGDM++L P N A
Sbjct: 62 KSLHFLKIKHGDMLFLFPSNSA 83
Score = 37.9 bits (84), Expect = 0.19
Identities = 16/28 (57%), Positives = 18/28 (64%)
Frame = +1
Query: 430 LYKVSGLIERXRDEKLCRHNLNGKCVHC 513
L K G I R RD +LC+H GKCVHC
Sbjct: 116 LSKQDGKIYRNRDPQLCQHGPMGKCVHC 143
Score = 37.5 bits (83), Expect = 0.26
Identities = 16/30 (53%), Positives = 22/30 (73%), Gaps = 2/30 (6%)
Frame = +3
Query: 516 PLXPWDEXYLKEHN--IKHMSFHSYLPKIT 599
PL P+DE YL + +KHMSFH+Y+ K+T
Sbjct: 145 PLEPFDEDYLNHLDPPVKHMSFHAYIRKLT 174
>UniRef50_UPI0000D9E56B Cluster: PREDICTED: similar to Nuclear
protein localization protein 4 homolog (Protein NPL4);
n=1; Macaca mulatta|Rep: PREDICTED: similar to Nuclear
protein localization protein 4 homolog (Protein NPL4) -
Macaca mulatta
Length = 323
Score = 50.4 bits (115), Expect = 3e-05
Identities = 23/92 (25%), Positives = 50/92 (54%)
Frame = +1
Query: 16 SQKMLLRVQSPEGTARVEVLDSDVTAHLFXRIYEALNLNSFAFTLHKDRQRKEEITSSKS 195
++ +++RVQSP+G R+ + A ++ + + F+++ +R + EIT+S +
Sbjct: 196 AESIIIRVQSPDGVKRITATKRETAATFLKKVAKEFGFQNNGFSVYINRNKTGEITASSN 255
Query: 196 RQLRDCGLCHGDMIYLNPVNGAVLFEQSSTSS 291
+ L + HGD+++L P + A + TS+
Sbjct: 256 KSLNLLKIKHGDLLFLFPSSLAGPSSEMETSA 287
>UniRef50_Q8TAT6 Cluster: Nuclear protein localization protein 4
homolog; n=42; Eumetazoa|Rep: Nuclear protein
localization protein 4 homolog - Homo sapiens (Human)
Length = 608
Score = 50.0 bits (114), Expect = 4e-05
Identities = 23/91 (25%), Positives = 49/91 (53%)
Frame = +1
Query: 16 SQKMLLRVQSPEGTARVEVLDSDVTAHLFXRIYEALNLNSFAFTLHKDRQRKEEITSSKS 195
++ +++RVQSP+G R+ + A ++ + + F+++ +R + EIT+S +
Sbjct: 2 AESIIIRVQSPDGVKRITATKRETAATFLKKVAKEFGFQNNGFSVYINRNKTGEITASSN 61
Query: 196 RQLRDCGLCHGDMIYLNPVNGAVLFEQSSTS 288
+ L + HGD+++L P + A + TS
Sbjct: 62 KSLNLLKIKHGDLLFLFPSSLAGPSSEMETS 92
Score = 39.5 bits (88), Expect = 0.063
Identities = 17/28 (60%), Positives = 18/28 (64%)
Frame = +1
Query: 430 LYKVSGLIERXRDEKLCRHNLNGKCVHC 513
L K G I R RD +LCRH GKCVHC
Sbjct: 114 LSKQDGKIYRSRDPQLCRHGPLGKCVHC 141
Score = 38.7 bits (86), Expect = 0.11
Identities = 17/30 (56%), Positives = 22/30 (73%), Gaps = 2/30 (6%)
Frame = +3
Query: 516 PLXPWDEXYLK--EHNIKHMSFHSYLPKIT 599
PL P+DE YL E +KHMSFH+Y+ K+T
Sbjct: 143 PLEPFDEDYLNHLEPPVKHMSFHAYIRKLT 172
>UniRef50_Q6C619 Cluster: Yarrowia lipolytica chromosome E of strain
CLIB 122 of Yarrowia lipolytica; n=1; Yarrowia
lipolytica|Rep: Yarrowia lipolytica chromosome E of
strain CLIB 122 of Yarrowia lipolytica - Yarrowia
lipolytica (Candida lipolytica)
Length = 561
Score = 49.6 bits (113), Expect = 6e-05
Identities = 18/30 (60%), Positives = 24/30 (80%)
Frame = +3
Query: 516 PLXPWDEXYLKEHNIKHMSFHSYLPKITSK 605
P+ PWDE YLKE IKHMS+H+Y+ K+ S+
Sbjct: 143 PVDPWDEEYLKEQKIKHMSYHAYVKKLNSQ 172
>UniRef50_Q9P780 Cluster: Cdc48-Ufd1-Npl4 complex component Npl4;
n=1; Schizosaccharomyces pombe|Rep: Cdc48-Ufd1-Npl4
complex component Npl4 - Schizosaccharomyces pombe
(Fission yeast)
Length = 545
Score = 45.2 bits (102), Expect = 0.001
Identities = 18/29 (62%), Positives = 23/29 (79%)
Frame = +3
Query: 516 PLXPWDEXYLKEHNIKHMSFHSYLPKITS 602
PL P+DE Y +E+ IKH+SFH+YL KI S
Sbjct: 155 PLEPYDESYRQENKIKHLSFHAYLRKINS 183
Score = 39.1 bits (87), Expect = 0.084
Identities = 26/77 (33%), Positives = 41/77 (53%), Gaps = 4/77 (5%)
Frame = +1
Query: 25 MLLRVQSPEGTARVEVLDSDVTAHLFXRIYEALNLNSFA---FTLHKDRQRKEEITSSKS 195
M+LR +S G AR E +D A L +I + N ++ +L ++ + I S+ +
Sbjct: 1 MILRFRSKRGMARAEFQPTDTLAMLSAKILSDILKNDYSPENVSLCQNESDQGVIFSNLN 60
Query: 196 RQ-LRDCGLCHGDMIYL 243
Q L+D GL HG M+YL
Sbjct: 61 DQTLQDAGLTHGQMLYL 77
>UniRef50_P33755 Cluster: Protein NPL4; n=6; Saccharomycetales|Rep:
Protein NPL4 - Saccharomyces cerevisiae (Baker's yeast)
Length = 580
Score = 43.2 bits (97), Expect = 0.005
Identities = 16/27 (59%), Positives = 22/27 (81%)
Frame = +3
Query: 516 PLXPWDEXYLKEHNIKHMSFHSYLPKI 596
PL PWD+ Y +++ IKH+SFHSYL K+
Sbjct: 150 PLPPWDKEYHEKNKIKHISFHSYLKKL 176
>UniRef50_Q95QZ9 Cluster: Putative uncharacterized protein; n=5;
Caenorhabditis|Rep: Putative uncharacterized protein -
Caenorhabditis elegans
Length = 529
Score = 42.7 bits (96), Expect = 0.007
Identities = 19/36 (52%), Positives = 27/36 (75%), Gaps = 1/36 (2%)
Frame = +3
Query: 516 PLXPWDEXYLKEHNIKHMSFHSYLPKIT-SKNFGVT 620
P+ P+DE YLKE +IKHMSFH+++ K+ S+ G T
Sbjct: 46 PVDPFDEEYLKEKDIKHMSFHAHVRKLLGSQGKGTT 81
>UniRef50_A3GFS1 Cluster: Nuclear protein localization factor and ER
translocation component; n=6; Saccharomycetales|Rep:
Nuclear protein localization factor and ER translocation
component - Pichia stipitis (Yeast)
Length = 577
Score = 42.7 bits (96), Expect = 0.007
Identities = 16/27 (59%), Positives = 21/27 (77%)
Frame = +3
Query: 516 PLXPWDEXYLKEHNIKHMSFHSYLPKI 596
PL PWD Y KE+ IKHMS+H+YL ++
Sbjct: 159 PLPPWDANYRKENAIKHMSYHAYLKEL 185
>UniRef50_A1CS06 Cluster: Endoplasmic reticulum and nuclear membrane
proteinc Npl4, putative; n=16; Pezizomycotina|Rep:
Endoplasmic reticulum and nuclear membrane proteinc
Npl4, putative - Aspergillus clavatus
Length = 674
Score = 42.3 bits (95), Expect = 0.009
Identities = 17/29 (58%), Positives = 21/29 (72%)
Frame = +3
Query: 516 PLXPWDEXYLKEHNIKHMSFHSYLPKITS 602
PL P+D YL E IKH+SFHSY+ KI +
Sbjct: 178 PLEPYDPKYLAEKKIKHLSFHSYMRKINA 206
Score = 33.1 bits (72), Expect = 5.5
Identities = 13/29 (44%), Positives = 18/29 (62%)
Frame = +1
Query: 427 QLYKVSGLIERXRDEKLCRHNLNGKCVHC 513
+L K G I+R RD K+C+H G C +C
Sbjct: 148 KLDKKDGKIQRPRDMKMCKHGPKGMCDYC 176
>UniRef50_Q4PGP9 Cluster: Putative uncharacterized protein; n=1;
Ustilago maydis|Rep: Putative uncharacterized protein -
Ustilago maydis (Smut fungus)
Length = 731
Score = 41.1 bits (92), Expect = 0.021
Identities = 16/26 (61%), Positives = 20/26 (76%)
Frame = +3
Query: 516 PLXPWDEXYLKEHNIKHMSFHSYLPK 593
PL P+D Y E+NIKH+SFH+YL K
Sbjct: 168 PLEPYDSKYQTENNIKHLSFHAYLRK 193
>UniRef50_Q5KKN9 Cluster: ER-associated protein catabolism-related
protein, putative; n=2; Filobasidiella neoformans|Rep:
ER-associated protein catabolism-related protein,
putative - Cryptococcus neoformans (Filobasidiella
neoformans)
Length = 693
Score = 40.7 bits (91), Expect = 0.027
Identities = 15/29 (51%), Positives = 21/29 (72%)
Frame = +3
Query: 516 PLXPWDEXYLKEHNIKHMSFHSYLPKITS 602
PL P+D + EH IKH+S+H+YL K+ S
Sbjct: 171 PLEPYDPKFQSEHQIKHLSYHAYLRKLLS 199
>UniRef50_A5DBC9 Cluster: Putative uncharacterized protein; n=1;
Pichia guilliermondii|Rep: Putative uncharacterized
protein - Pichia guilliermondii (Yeast) (Candida
guilliermondii)
Length = 564
Score = 40.3 bits (90), Expect = 0.036
Identities = 15/30 (50%), Positives = 22/30 (73%)
Frame = +3
Query: 516 PLXPWDEXYLKEHNIKHMSFHSYLPKITSK 605
PL WD+ Y +E+ IKHMSFH+Y+ +I +
Sbjct: 179 PLPSWDKGYREENGIKHMSFHAYVNQINEQ 208
Score = 33.5 bits (73), Expect = 4.2
Identities = 22/76 (28%), Positives = 40/76 (52%), Gaps = 4/76 (5%)
Frame = +1
Query: 28 LLRVQSPEGTARV----EVLDSDVTAHLFXRIYEALNLNSFAFTLHKDRQRKEEITSSKS 195
++R ++P G RV E +D+ L ++ E+ +L+SF F+ K + +
Sbjct: 26 IIRFRTPSGMLRVNATPETAFNDLLNDLGNQMGES-DLSSFTFS-DKPNDKGSSANTFHG 83
Query: 196 RQLRDCGLCHGDMIYL 243
+ + D GL HGDM+Y+
Sbjct: 84 KSVADLGLKHGDMLYV 99
>UniRef50_Q8SRJ9 Cluster: PROTEIN INVOLVED IN ER TRANSLOCATION; n=1;
Encephalitozoon cuniculi|Rep: PROTEIN INVOLVED IN ER
TRANSLOCATION - Encephalitozoon cuniculi
Length = 505
Score = 39.5 bits (88), Expect = 0.063
Identities = 16/30 (53%), Positives = 21/30 (70%)
Frame = +3
Query: 516 PLXPWDEXYLKEHNIKHMSFHSYLPKITSK 605
PL PWDE Y K++ IK++SF SY + SK
Sbjct: 107 PLDPWDEKYYKDNMIKYLSFGSYCEMMKSK 136
>UniRef50_Q4S8H6 Cluster: Chromosome 2 SCAF14705, whole genome
shotgun sequence; n=2; Euteleostomi|Rep: Chromosome 2
SCAF14705, whole genome shotgun sequence - Tetraodon
nigroviridis (Green puffer)
Length = 584
Score = 38.3 bits (85), Expect = 0.15
Identities = 17/30 (56%), Positives = 22/30 (73%), Gaps = 2/30 (6%)
Frame = +3
Query: 516 PLXPWDEXYLKEHN--IKHMSFHSYLPKIT 599
PL P+DE YL + +KHMSFH+YL K+T
Sbjct: 49 PLEPFDEDYLNHLDPPVKHMSFHAYLRKLT 78
Score = 36.7 bits (81), Expect = 0.45
Identities = 15/28 (53%), Positives = 17/28 (60%)
Frame = +1
Query: 430 LYKVSGLIERXRDEKLCRHNLNGKCVHC 513
L K G I R +D +LC H GKCVHC
Sbjct: 20 LAKQDGKIYRNKDPQLCHHGALGKCVHC 47
>UniRef50_A2FMY9 Cluster: Putative uncharacterized protein; n=1;
Trichomonas vaginalis G3|Rep: Putative uncharacterized
protein - Trichomonas vaginalis G3
Length = 253
Score = 35.1 bits (77), Expect = 1.4
Identities = 12/32 (37%), Positives = 20/32 (62%)
Frame = +3
Query: 513 YPLXPWDEXYLKEHNIKHMSFHSYLPKITSKN 608
YP+ PW E Y K H+I+++SF + ++N
Sbjct: 13 YPIFPWVETYFKSHSIRNLSFPITMQSTKNRN 44
>UniRef50_UPI0000D9E5FA Cluster: PREDICTED: similar to nuclear
protein localization 4, partial; n=1; Macaca
mulatta|Rep: PREDICTED: similar to nuclear protein
localization 4, partial - Macaca mulatta
Length = 81
Score = 34.7 bits (76), Expect = 1.8
Identities = 15/27 (55%), Positives = 20/27 (74%), Gaps = 2/27 (7%)
Frame = +3
Query: 525 PWDEXYLK--EHNIKHMSFHSYLPKIT 599
P+DE YL E +KHMSFH+Y+ K+T
Sbjct: 51 PFDEDYLNHLEPPVKHMSFHAYIRKLT 77
>UniRef50_Q1NLM2 Cluster: Putative uncharacterized protein; n=1;
delta proteobacterium MLMS-1|Rep: Putative
uncharacterized protein - delta proteobacterium MLMS-1
Length = 309
Score = 34.3 bits (75), Expect = 2.4
Identities = 18/39 (46%), Positives = 22/39 (56%), Gaps = 1/39 (2%)
Frame = -1
Query: 417 FXFLWSTRRWGGYW*CDFRRGRTWL**LXW-SGTFTEGF 304
F FL R+ GGYW CDF++G + W SGT GF
Sbjct: 271 FIFLADERKPGGYWFCDFKQGTVF-----WASGTIPGGF 304
>UniRef50_Q9W0J2 Cluster: CG13913-PA; n=3; Diptera|Rep: CG13913-PA -
Drosophila melanogaster (Fruit fly)
Length = 836
Score = 32.7 bits (71), Expect = 7.3
Identities = 16/41 (39%), Positives = 24/41 (58%)
Frame = -2
Query: 272 SKSTAPLTGFKYIMSP*HSPQSRSCLDLLDVISSLRCLSLC 150
S + AP+ G ++ +PQS LLD +S L+CLS+C
Sbjct: 189 SNAPAPMPGASSSLAMNRNPQSYQRRALLDELSCLQCLSIC 229
>UniRef50_UPI0000F1FDE7 Cluster: PREDICTED: similar to BC055004
protein; n=5; Danio rerio|Rep: PREDICTED: similar to
BC055004 protein - Danio rerio
Length = 550
Score = 32.3 bits (70), Expect = 9.6
Identities = 11/24 (45%), Positives = 14/24 (58%)
Frame = +2
Query: 11 LXHKKCYCEFSHPKVQREWRCWTP 82
L K C CE+S P+ + WRC P
Sbjct: 224 LGTKNCCCEYSDPRTRETWRCKKP 247
>UniRef50_A0XZT4 Cluster: Putative uncharacterized protein; n=1;
Alteromonadales bacterium TW-7|Rep: Putative
uncharacterized protein - Alteromonadales bacterium TW-7
Length = 123
Score = 32.3 bits (70), Expect = 9.6
Identities = 17/42 (40%), Positives = 24/42 (57%)
Frame = -1
Query: 267 EYSTIDWIQVYHVAMTQSTISQLSRFAGCDFFLALSVLVQSE 142
EYS + +Q + +TQST+S+L F G D F L V +E
Sbjct: 8 EYSPLLEVQDQTLIITQSTLSELKSFKGSDLFAELPGSVPNE 49
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 572,374,452
Number of Sequences: 1657284
Number of extensions: 10374662
Number of successful extensions: 26559
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 25789
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 26557
length of database: 575,637,011
effective HSP length: 97
effective length of database: 414,880,463
effective search space used: 45221970467
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -