Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fprWP02_F_H15
(665 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g77440.1 68414.m09018 20S proteasome beta subunit C (PBC2) id... 212 2e-55
At1g21720.1 68414.m02719 20S proteasome beta subunit C1 (PBC1) (... 211 4e-55
At3g60820.1 68416.m06804 20S proteasome beta subunit F1 (PBF1) 62 3e-10
At3g26340.1 68416.m03286 20S proteasome beta subunit E, putative... 44 7e-05
At4g31300.1 68417.m04441 20S proteasome beta subunit A (PBA1) (P... 44 9e-05
At1g13060.1 68414.m01514 20S proteasome beta subunit E1 (PBE1) (... 44 1e-04
At5g40580.2 68418.m04925 20S proteasome beta subunit B (PBB2) (P... 34 0.074
At5g40580.1 68418.m04924 20S proteasome beta subunit B (PBB2) (P... 34 0.074
At3g27430.2 68416.m03429 20S proteasome beta subunit B (PBB1) id... 33 0.17
At3g27430.1 68416.m03428 20S proteasome beta subunit B (PBB1) id... 33 0.17
At5g54080.2 68418.m06733 homogentisate 1,2-dioxygenase / homogen... 29 2.8
At5g54080.1 68418.m06732 homogentisate 1,2-dioxygenase / homogen... 29 2.8
At3g45460.1 68416.m04909 hypothetical protein 28 4.9
At1g27660.1 68414.m03381 ethylene-responsive protein -related co... 28 6.4
At5g57035.1 68418.m07119 protein kinase family protein contains ... 27 8.5
At3g49300.1 68416.m05388 proline-rich family protein contains pr... 27 8.5
>At1g77440.1 68414.m09018 20S proteasome beta subunit C (PBC2)
identical to residues 14-204 of 20S proteasome beta
subunit PBC2 GB:AAC32069 [Arabidopsis thaliana]
Length = 204
Score = 212 bits (517), Expect = 2e-55
Identities = 95/181 (52%), Positives = 138/181 (76%)
Frame = +2
Query: 122 MSILXYNGGAVVAMKGQDCVAIATDKRFGIQAQTVSTNFPKVFQMGPTLYVGLPGLATDT 301
MSI YNG AVVAM G++C AIA+D+R G+Q QT++T+F ++ ++ L++GL GLATD
Sbjct: 1 MSIFEYNGSAVVAMVGKNCFAIASDRRLGVQLQTIATDFQRISKIHDHLFIGLSGLATDV 60
Query: 302 QTVFXRLKFXMNLYELKENRMMRPKTFSAMLSNLLYERRFGPYFIEPVIAGLXPYDNQPY 481
QT++ RL F LY+L+E R M+P+TF++++S +LYE+RFGP+ +PVIAGL DN+P+
Sbjct: 61 QTLYQRLVFRHKLYQLREERDMKPETFASLVSAILYEKRFGPFLCQPVIAGLGD-DNKPF 119
Query: 482 VCNMDLIGCPNEPEXFVVSGTCSEQLYGMCEALWEPNLKPDELFXTISQALVNAADXDAI 661
+C MD IG + FVVSGT SE LYG CEA+++P+++ +ELF TISQAL+++ D D +
Sbjct: 120 ICTMDSIGAKELAKDFVVSGTASESLYGACEAMFKPDMEAEELFETISQALLSSVDRDCL 179
Query: 662 S 664
S
Sbjct: 180 S 180
>At1g21720.1 68414.m02719 20S proteasome beta subunit C1 (PBC1)
(PRCT) almost identical to GB:AAC32069 from [Arabidopsis
thaliana], EST gb|T76747 comes from this gene; identical
to cDNA proteasome subunit prct GI:2511567
Length = 204
Score = 211 bits (515), Expect = 4e-55
Identities = 94/181 (51%), Positives = 138/181 (76%)
Frame = +2
Query: 122 MSILXYNGGAVVAMKGQDCVAIATDKRFGIQAQTVSTNFPKVFQMGPTLYVGLPGLATDT 301
MSI YNG AVVAM G++C AIA+D+R G+Q QT++T+F ++ ++ +++GL GLATD
Sbjct: 1 MSIFEYNGSAVVAMVGKNCFAIASDRRLGVQLQTIATDFQRISKIHDRVFIGLSGLATDV 60
Query: 302 QTVFXRLKFXMNLYELKENRMMRPKTFSAMLSNLLYERRFGPYFIEPVIAGLXPYDNQPY 481
QT++ RL F LY+L+E R M+P+TF++++S +LYE+RFGPY +PVIAGL D++P+
Sbjct: 61 QTLYQRLVFRHKLYQLREERDMKPETFASLVSAILYEKRFGPYLCQPVIAGLGD-DDKPF 119
Query: 482 VCNMDLIGCPNEPEXFVVSGTCSEQLYGMCEALWEPNLKPDELFXTISQALVNAADXDAI 661
+C MD IG + FVVSGT SE LYG CEA+++P+++ +ELF TISQAL+++ D D +
Sbjct: 120 ICTMDSIGAKELAKDFVVSGTASESLYGACEAMYKPDMEAEELFETISQALLSSVDRDCL 179
Query: 662 S 664
S
Sbjct: 180 S 180
>At3g60820.1 68416.m06804 20S proteasome beta subunit F1 (PBF1)
Length = 223
Score = 62.1 bits (144), Expect = 3e-10
Identities = 37/129 (28%), Positives = 59/129 (45%)
Frame = +2
Query: 119 NMSILXYNGGAVVAMKGQDCVAIATDKRFGIQAQTVSTNFPKVFQMGPTLYVGLPGLATD 298
N S NGG VA+ G D IA D R +S ++ K+ ++ + G D
Sbjct: 7 NWSPYDNNGGTCVAIAGSDYCVIAADTRMSTGYSILSRDYSKIHKLADRAVLSSSGFQAD 66
Query: 299 TQTVFXRLKFXMNLYELKENRMMRPKTFSAMLSNLLYERRFGPYFIEPVIAGLXPYDNQP 478
+ + LK +Y+ + N+ M + +LSN LY +RF PY+ V+ GL + +
Sbjct: 67 VKALQKVLKSRHLIYQHQHNKQMSCPAMAQLLSNTLYFKRFFPYYAFNVLGGLDE-EGKG 125
Query: 479 YVCNMDLIG 505
V D +G
Sbjct: 126 CVFTYDAVG 134
>At3g26340.1 68416.m03286 20S proteasome beta subunit E, putative
very strong similarity to SP|O23717 Proteasome subunit
beta type 5 precursor (EC 3.4.25.1) (20S proteasome
subunit E) (Proteasome epsilon chain) {Arabidopsis
thaliana}
Length = 273
Score = 44.4 bits (100), Expect = 7e-05
Identities = 40/174 (22%), Positives = 76/174 (43%)
Frame = +2
Query: 143 GGAVVAMKGQDCVAIATDKRFGIQAQTVSTNFPKVFQMGPTLYVGLPGLATDTQTVFXRL 322
G +A ++ V +A D R + S + K+ ++ P + + G A D Q L
Sbjct: 57 GTTTLAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNL 116
Query: 323 KFXMNLYELKENRMMRPKTFSAMLSNLLYERRFGPYFIEPVIAGLXPYDNQPYVCNMDLI 502
L+EL R + S +L+N+LY R + +IAG + P + +D
Sbjct: 117 GIKCRLHELANKRRISVSGASKLLANMLYSYRGMGLSVGTMIAGWD--ETGPGLYYVDNE 174
Query: 503 GCPNEPEXFVVSGTCSEQLYGMCEALWEPNLKPDELFXTISQALVNAADXDAIS 664
G + + F V G+ S YG+ ++ ++ ++ +E +++ +A D S
Sbjct: 175 GGRLKGDRFSV-GSGSPYAYGVLDSGYKFDMSVEEASELARRSIYHATFRDGAS 227
>At4g31300.1 68417.m04441 20S proteasome beta subunit A (PBA1)
(PRCD) identical to cDNA proteasome subunit prcd
GI:2511593
Length = 233
Score = 44.0 bits (99), Expect = 9e-05
Identities = 34/174 (19%), Positives = 71/174 (40%)
Frame = +2
Query: 143 GGAVVAMKGQDCVAIATDKRFGIQAQTVSTNFPKVFQMGPTLYVGLPGLATDTQTVFXRL 322
G ++ + V + D R + K+ Q+ +YV G A D+Q V +
Sbjct: 12 GTTIIGVTYNGGVVLGADSRTSTGMYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYV 71
Query: 323 KFXMNLYELKENRMMRPKTFSAMLSNLLYERRFGPYFIEPVIAGLXPYDNQPYVCNMDLI 502
++ ++ + ++ + K + ++ L Y + ++ G Y+ + + L
Sbjct: 72 RYFLHQHTIQHGQPATVKVSANLIRMLAYNNK-NMLQTGLIVGGWDKYEGGK-IYGIPLG 129
Query: 503 GCPNEPEXFVVSGTCSEQLYGMCEALWEPNLKPDELFXTISQALVNAADXDAIS 664
G E + F + G+ S LYG + W+ N+ +E + +A+ A D S
Sbjct: 130 GTVVE-QPFAIGGSGSSYLYGFFDQAWKDNMTKEEAEQLVVKAVSLAIARDGAS 182
>At1g13060.1 68414.m01514 20S proteasome beta subunit E1 (PBE1)
(PRCE) identical to GB:O23717; identical to cDNA
proteasome subunit prce GI:2511595
Length = 274
Score = 43.6 bits (98), Expect = 1e-04
Identities = 38/162 (23%), Positives = 71/162 (43%)
Frame = +2
Query: 179 VAIATDKRFGIQAQTVSTNFPKVFQMGPTLYVGLPGLATDTQTVFXRLKFXMNLYELKEN 358
V +A D R + S + K+ ++ P + + G A D Q L L+EL
Sbjct: 69 VMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCRLHELANK 128
Query: 359 RMMRPKTFSAMLSNLLYERRFGPYFIEPVIAGLXPYDNQPYVCNMDLIGCPNEPEXFVVS 538
R + S +L+N+LY R + +IAG + P + +D G + + F V
Sbjct: 129 RRISVSGASKLLANMLYSYRGMGLSVGTMIAGWD--ETGPGLYYVDNEGGRLKGDRFSV- 185
Query: 539 GTCSEQLYGMCEALWEPNLKPDELFXTISQALVNAADXDAIS 664
G+ S YG+ ++ ++ ++ +E +++ +A D S
Sbjct: 186 GSGSPYAYGVLDSGYKYDMSVEEASELARRSIYHATFRDGAS 227
>At5g40580.2 68418.m04925 20S proteasome beta subunit B (PBB2)
(PRCFC) identical to 20S proteasome beta subunit PBB2
[Arabidopsis thaliana] GI:3421104, cDNA proteasome
subunit prcfc GI:2511575
Length = 274
Score = 34.3 bits (75), Expect = 0.074
Identities = 37/171 (21%), Positives = 66/171 (38%)
Frame = +2
Query: 143 GGAVVAMKGQDCVAIATDKRFGIQAQTVSTNFPKVFQMGPTLYVGLPGLATDTQTVFXRL 322
G +V + +D V + D R N K+ M P +Y G A DT+ V +
Sbjct: 39 GTTIVGLIFKDGVILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADTEAVTDMV 98
Query: 323 KFXMNLYELKENRMMRPKTFSAMLSNLLYERRFGPYFIEPVIAGLXPYDNQPYVCNMDLI 502
+ L+ + R R T +L L+ + G V+ G+ P++ +
Sbjct: 99 SSQLRLHRYQTGRDSRVVTALTLLKKHLFSYQ-GHVSAALVLGGVD--ITGPHLHTIYPH 155
Query: 503 GCPNEPEXFVVSGTCSEQLYGMCEALWEPNLKPDELFXTISQALVNAADXD 655
G + F G+ S + EA ++ L DE +++A+ + D
Sbjct: 156 G-STDTLPFATMGSGSLAAMSVFEAKYKEGLTRDEGIKLVAEAICSGIFND 205
>At5g40580.1 68418.m04924 20S proteasome beta subunit B (PBB2)
(PRCFC) identical to 20S proteasome beta subunit PBB2
[Arabidopsis thaliana] GI:3421104, cDNA proteasome
subunit prcfc GI:2511575
Length = 274
Score = 34.3 bits (75), Expect = 0.074
Identities = 37/171 (21%), Positives = 66/171 (38%)
Frame = +2
Query: 143 GGAVVAMKGQDCVAIATDKRFGIQAQTVSTNFPKVFQMGPTLYVGLPGLATDTQTVFXRL 322
G +V + +D V + D R N K+ M P +Y G A DT+ V +
Sbjct: 39 GTTIVGLIFKDGVILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADTEAVTDMV 98
Query: 323 KFXMNLYELKENRMMRPKTFSAMLSNLLYERRFGPYFIEPVIAGLXPYDNQPYVCNMDLI 502
+ L+ + R R T +L L+ + G V+ G+ P++ +
Sbjct: 99 SSQLRLHRYQTGRDSRVVTALTLLKKHLFSYQ-GHVSAALVLGGVD--ITGPHLHTIYPH 155
Query: 503 GCPNEPEXFVVSGTCSEQLYGMCEALWEPNLKPDELFXTISQALVNAADXD 655
G + F G+ S + EA ++ L DE +++A+ + D
Sbjct: 156 G-STDTLPFATMGSGSLAAMSVFEAKYKEGLTRDEGIKLVAEAICSGIFND 205
>At3g27430.2 68416.m03429 20S proteasome beta subunit B (PBB1)
identical to 20S proteasome beta subunit PBB1 (PBB1)
GB:AAC32066 [Arabidopsis thaliana] (Genetics 149 (2),
677-692 (1998)); contains Pfam profile: PF00227
proteasome A-type and B-type;
Length = 273
Score = 33.1 bits (72), Expect = 0.17
Identities = 22/89 (24%), Positives = 35/89 (39%)
Frame = +2
Query: 143 GGAVVAMKGQDCVAIATDKRFGIQAQTVSTNFPKVFQMGPTLYVGLPGLATDTQTVFXRL 322
G +V + +D V + D R N K+ M P +Y G A DT+ V +
Sbjct: 39 GTTIVGLIFKDGVILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADTEAVTDMV 98
Query: 323 KFXMNLYELKENRMMRPKTFSAMLSNLLY 409
+ L+ + R R T +L L+
Sbjct: 99 SSQLRLHRYQTGRDSRVITALTLLKKHLF 127
>At3g27430.1 68416.m03428 20S proteasome beta subunit B (PBB1)
identical to 20S proteasome beta subunit PBB1 (PBB1)
GB:AAC32066 [Arabidopsis thaliana] (Genetics 149 (2),
677-692 (1998)); contains Pfam profile: PF00227
proteasome A-type and B-type;
Length = 267
Score = 33.1 bits (72), Expect = 0.17
Identities = 22/89 (24%), Positives = 35/89 (39%)
Frame = +2
Query: 143 GGAVVAMKGQDCVAIATDKRFGIQAQTVSTNFPKVFQMGPTLYVGLPGLATDTQTVFXRL 322
G +V + +D V + D R N K+ M P +Y G A DT+ V +
Sbjct: 39 GTTIVGLIFKDGVILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADTEAVTDMV 98
Query: 323 KFXMNLYELKENRMMRPKTFSAMLSNLLY 409
+ L+ + R R T +L L+
Sbjct: 99 SSQLRLHRYQTGRDSRVITALTLLKKHLF 127
>At5g54080.2 68418.m06733 homogentisate 1,2-dioxygenase /
homogentisicase/homogentisate oxygenase / homogentisic
acid oxidase (HGO) identical to SP|Q9ZRA2 Homogentisate
1,2-dioxygenase (EC 1.13.11.5) (Homogentisicase)
(Homogentisate oxygenase) (Homogentisic acid oxidase)
{Arabidopsis thaliana}; contains Pfam profile PF04209:
homogentisate 1,2-dioxygenase
Length = 461
Score = 29.1 bits (62), Expect = 2.8
Identities = 14/33 (42%), Positives = 17/33 (51%), Gaps = 1/33 (3%)
Frame = +2
Query: 392 LSNLLYERRFGPYFIEPVIAGLXPYD-NQPYVC 487
L L Y+ FG +F IAG P D N P +C
Sbjct: 8 LEELKYQSGFGNHFSSEAIAGALPLDQNSPLLC 40
>At5g54080.1 68418.m06732 homogentisate 1,2-dioxygenase /
homogentisicase/homogentisate oxygenase / homogentisic
acid oxidase (HGO) identical to SP|Q9ZRA2 Homogentisate
1,2-dioxygenase (EC 1.13.11.5) (Homogentisicase)
(Homogentisate oxygenase) (Homogentisic acid oxidase)
{Arabidopsis thaliana}; contains Pfam profile PF04209:
homogentisate 1,2-dioxygenase
Length = 461
Score = 29.1 bits (62), Expect = 2.8
Identities = 14/33 (42%), Positives = 17/33 (51%), Gaps = 1/33 (3%)
Frame = +2
Query: 392 LSNLLYERRFGPYFIEPVIAGLXPYD-NQPYVC 487
L L Y+ FG +F IAG P D N P +C
Sbjct: 8 LEELKYQSGFGNHFSSEAIAGALPLDQNSPLLC 40
>At3g45460.1 68416.m04909 hypothetical protein
Length = 315
Score = 28.3 bits (60), Expect = 4.9
Identities = 16/58 (27%), Positives = 30/58 (51%), Gaps = 1/58 (1%)
Frame = +2
Query: 494 DLIGCPNEPEXFVVS-GTCSEQLYGMCEALWEPNLKPDELFXTISQALVNAADXDAIS 664
D+ CP++ ++S +CS L E +WE +K + + + A +NAA A++
Sbjct: 210 DVPRCPHDGCKSILSLKSCSHLLTPKLEEMWEHRIKEEFIPMELGDAALNAAKPFALT 267
>At1g27660.1 68414.m03381 ethylene-responsive protein -related
contains similarity to ethylene-inducible ER33 protein
[Lycopersicon esculentum] gi|5669656|gb|AAD46413
Length = 453
Score = 27.9 bits (59), Expect = 6.4
Identities = 12/39 (30%), Positives = 18/39 (46%)
Frame = -3
Query: 621 IVSKSSSGFKLGSQRASHIPYSCSEQVPDTTKXSGSFGQ 505
++ SSGF + S +P S S P + G+F Q
Sbjct: 161 LLKSMSSGFPINGDYGSSLPSSSSSSSPSSQSHRGNFSQ 199
>At5g57035.1 68418.m07119 protein kinase family protein contains
Pfam profile: PF00069 Eukaryotic protein kinase domain
Length = 786
Score = 27.5 bits (58), Expect = 8.5
Identities = 10/24 (41%), Positives = 15/24 (62%)
Frame = -1
Query: 236 SWLILFELVYQTACP*RWPHSLGP 165
SW I F ++Y+TAC + H+ P
Sbjct: 514 SWFIRFRIIYETACGLAFLHNSKP 537
>At3g49300.1 68416.m05388 proline-rich family protein contains
proline-rich extensin domains, INTERPRO:IPR002965
Length = 86
Score = 27.5 bits (58), Expect = 8.5
Identities = 11/24 (45%), Positives = 16/24 (66%)
Frame = +1
Query: 181 GHRYGQAVWYTSSNSINQLPKSIP 252
G R G V +T NS+N++ +SIP
Sbjct: 23 GFREGHGVTHTDQNSLNKVEESIP 46
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,586,808
Number of Sequences: 28952
Number of extensions: 294704
Number of successful extensions: 671
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 659
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 669
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1403159472
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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