Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fprWP02_F_G20
(625 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_P22087 Cluster: rRNA 2'-O-methyltransferase fibrillarin... 110 3e-23
UniRef50_Q6AT27 Cluster: Putative fibrillarin protein; n=2; Oryz... 99 7e-20
UniRef50_UPI0000DD7D0A Cluster: PREDICTED: similar to fibrillari... 97 3e-19
UniRef50_Q2PET2 Cluster: Putative fibrillarin homolog; n=1; Trif... 72 1e-11
UniRef50_A5AT06 Cluster: Putative uncharacterized protein; n=1; ... 71 2e-11
UniRef50_Q9VG21 Cluster: CG10909-PA; n=2; Sophophora|Rep: CG1090... 68 2e-10
UniRef50_Q3LWE7 Cluster: Nucleolar protein fibrillarin; n=1; Big... 64 3e-09
UniRef50_Q4QDL1 Cluster: Fibrillarin, putative; n=3; Leishmania|... 59 7e-08
UniRef50_Q74N89 Cluster: Fibrillarin-like rRNA/tRNA 2'-O-methylt... 47 4e-04
UniRef50_A7DP76 Cluster: Non-specific serine/threonine protein k... 44 0.002
UniRef50_UPI00015BB1B1 Cluster: rRNA 2'-O-methyltransferase fibr... 42 0.009
UniRef50_Q971W2 Cluster: Fibrillarin-like rRNA/tRNA 2'-O-methylt... 42 0.009
UniRef50_Q58108 Cluster: Fibrillarin-like rRNA/tRNA 2'-O-methylt... 41 0.021
UniRef50_A0RV24 Cluster: Fibrillarin-like rRNA methylase; n=1; C... 41 0.028
UniRef50_Q5A0V8 Cluster: Putative uncharacterized protein; n=1; ... 39 0.085
UniRef50_O27283 Cluster: Fibrillarin-like rRNA/tRNA 2'-O-methylt... 39 0.085
UniRef50_Q8TTT4 Cluster: Fibrillarin-like rRNA/tRNA 2'-O-methylt... 38 0.20
UniRef50_Q1FNR9 Cluster: Cobalamin (Vitamin B12) biosynthesis Cb... 32 9.7
UniRef50_Q11VL3 Cluster: Putative uncharacterized protein; n=1; ... 32 9.7
>UniRef50_P22087 Cluster: rRNA 2'-O-methyltransferase fibrillarin;
n=162; cellular organisms|Rep: rRNA
2'-O-methyltransferase fibrillarin - Homo sapiens
(Human)
Length = 321
Score = 110 bits (264), Expect = 3e-23
Identities = 49/74 (66%), Positives = 56/74 (75%)
Frame = +1
Query: 397 VIIEPHRHPGVFIXRGKXXALVTKNLVPXSXVYGEXXISLDNERDKVEYXVWNPFXSKLA 576
V++EPHRH GVFI RGK ALVTKNLVP VYGE +S+ DK+EY WNPF SKLA
Sbjct: 86 VMVEPHRHEGVFICRGKEDALVTKNLVPGESVYGEKRVSISEGDDKIEYRAWNPFRSKLA 145
Query: 577 AAIMGGVDAIHMAP 618
AAI+GGVD IH+ P
Sbjct: 146 AAILGGVDQIHIKP 159
>UniRef50_Q6AT27 Cluster: Putative fibrillarin protein; n=2; Oryza
sativa|Rep: Putative fibrillarin protein - Oryza sativa
subsp. japonica (Rice)
Length = 351
Score = 99.1 bits (236), Expect = 7e-20
Identities = 46/76 (60%), Positives = 56/76 (73%), Gaps = 1/76 (1%)
Frame = +1
Query: 394 QVIIEPHRHPGVFIXRGKXXALVTKNLVPXSXVYGEXXISLDNE-RDKVEYXVWNPFXSK 570
+V++ PH+H GVFI + K AL TKN+VP VYGE IS+ NE KVEY VWNPF SK
Sbjct: 70 KVVVVPHKHDGVFIAKAKEDALCTKNMVPGESVYGEKRISVQNEDGTKVEYRVWNPFRSK 129
Query: 571 LAAAIMGGVDAIHMAP 618
LAAA++GGVD I +AP
Sbjct: 130 LAAAVLGGVDNIWIAP 145
>UniRef50_UPI0000DD7D0A Cluster: PREDICTED: similar to fibrillarin;
n=6; Eutheria|Rep: PREDICTED: similar to fibrillarin -
Homo sapiens
Length = 498
Score = 97.1 bits (231), Expect = 3e-19
Identities = 45/76 (59%), Positives = 52/76 (68%)
Frame = +1
Query: 397 VIIEPHRHPGVFIXRGKXXALVTKNLVPXSXVYGEXXISLDNERDKVEYXVWNPFXSKLA 576
V +EPHRH GVFI RG ALVT N+VP VYGE +++ K EY WNPF SKLA
Sbjct: 262 VSVEPHRHEGVFIYRGAEDALVTLNMVPGQSVYGERRVTVTEGGVKQEYRTWNPFRSKLA 321
Query: 577 AAIMGGVDAIHMAPXS 624
AAI+GGVD IH+ P S
Sbjct: 322 AAILGGVDQIHIKPKS 337
>UniRef50_Q2PET2 Cluster: Putative fibrillarin homolog; n=1;
Trifolium pratense|Rep: Putative fibrillarin homolog -
Trifolium pratense (Red clover)
Length = 475
Score = 72.1 bits (169), Expect = 1e-11
Identities = 38/78 (48%), Positives = 48/78 (61%), Gaps = 1/78 (1%)
Frame = +1
Query: 394 QVIIEPHRHPGVFIXRGKXXALVTKNLVPXSXVYGEXXISLDNE-RDKVEYXVWNPFXSK 570
QV++ PHR G F +GK L T+NLVP +YGE I + NE +VEY V +P SK
Sbjct: 238 QVMVVPHRFEGFFNAKGKKNILCTRNLVPGEALYGEKLIHVQNEDGTEVEYRVCDPRRSK 297
Query: 571 LAAAIMGGVDAIHMAPXS 624
L AAI+GGV I + P S
Sbjct: 298 LGAAILGGVTNIWIKPGS 315
>UniRef50_A5AT06 Cluster: Putative uncharacterized protein; n=1;
Vitis vinifera|Rep: Putative uncharacterized protein -
Vitis vinifera (Grape)
Length = 516
Score = 70.9 bits (166), Expect = 2e-11
Identities = 36/65 (55%), Positives = 42/65 (64%), Gaps = 1/65 (1%)
Frame = +1
Query: 433 IXRGKXXALVTKNLVPXSXVYGEXXISLDNER-DKVEYXVWNPFXSKLAAAIMGGVDAIH 609
I K A VTKN+VP VY E IS+ NE K+EY VWNPF SKLAAAI+GGV I
Sbjct: 143 ISYDKEDARVTKNMVPGKAVYNEKKISVQNEDGSKIEYRVWNPFRSKLAAAILGGVHEIW 202
Query: 610 MAPXS 624
+ P +
Sbjct: 203 VKPSA 207
>UniRef50_Q9VG21 Cluster: CG10909-PA; n=2; Sophophora|Rep:
CG10909-PA - Drosophila melanogaster (Fruit fly)
Length = 349
Score = 68.1 bits (159), Expect = 2e-10
Identities = 32/72 (44%), Positives = 45/72 (62%), Gaps = 2/72 (2%)
Frame = +1
Query: 403 IEPHRHPGVFIXRGKXXA--LVTKNLVPXSXVYGEXXISLDNERDKVEYXVWNPFXSKLA 576
IEPHRH GV++ R + A L+T+N + YGE + + + E+ VW+PF SKLA
Sbjct: 114 IEPHRHYGVYLLRNRFDAIQLLTRNTSSSADDYGERRVISEYREMRCEFRVWSPFQSKLA 173
Query: 577 AAIMGGVDAIHM 612
A IMGGV +H+
Sbjct: 174 AGIMGGVSDLHL 185
>UniRef50_Q3LWE7 Cluster: Nucleolar protein fibrillarin; n=1;
Bigelowiella natans|Rep: Nucleolar protein fibrillarin -
Bigelowiella natans (Pedinomonas minutissima)
(Chlorarachnion sp.(strain CCMP 621))
Length = 253
Score = 63.7 bits (148), Expect = 3e-09
Identities = 32/73 (43%), Positives = 43/73 (58%), Gaps = 1/73 (1%)
Frame = +1
Query: 403 IEPHRHPGVFIXRGKXXALVTKNLVPXSXVYGEXXI-SLDNERDKVEYXVWNPFXSKLAA 579
IE HRH V++ RG + T N+ P GE + S N+R E VWNPF SKLAA
Sbjct: 11 IETHRHANVYVIRGIEDCIATINITPGKIFCGELLLKSFFNDRI-YELRVWNPFRSKLAA 69
Query: 580 AIMGGVDAIHMAP 618
A++ G+D+I + P
Sbjct: 70 AVLNGIDSIGLLP 82
>UniRef50_Q4QDL1 Cluster: Fibrillarin, putative; n=3;
Leishmania|Rep: Fibrillarin, putative - Leishmania major
Length = 297
Score = 59.3 bits (137), Expect = 7e-08
Identities = 33/78 (42%), Positives = 46/78 (58%), Gaps = 3/78 (3%)
Frame = +1
Query: 400 IIEPH-RHPGVFIXRGKXXALVTKNLVPXSXVYGEXXIS--LDNERDKVEYXVWNPFXSK 570
I PH R G ++ GK L T++LVP VY E ++ + E + E+ VWNP+ SK
Sbjct: 61 IFHPHARFNGCYLLAGKDT-LSTRSLVPGVSVYSEKRVNGTVAGESESNEFRVWNPYRSK 119
Query: 571 LAAAIMGGVDAIHMAPXS 624
LA+AI GV +I+M P S
Sbjct: 120 LASAIYAGVASIYMEPGS 137
>UniRef50_Q74N89 Cluster: Fibrillarin-like rRNA/tRNA
2'-O-methyltransferase; n=2; Archaea|Rep:
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase -
Nanoarchaeum equitans
Length = 232
Score = 46.8 bits (106), Expect = 4e-04
Identities = 28/65 (43%), Positives = 35/65 (53%)
Frame = +1
Query: 403 IEPHRHPGVFIXRGKXXALVTKNLVPXSXVYGEXXISLDNERDKVEYXVWNPFXSKLAAA 582
++PH+ GV+I K L+TKNLV VY E E EY W PF SKLA+A
Sbjct: 9 VKPHKLEGVYIGNNKF--LLTKNLVKGQKVYTERIFKSKGE----EYREWIPFRSKLASA 62
Query: 583 IMGGV 597
I G+
Sbjct: 63 IKKGL 67
>UniRef50_A7DP76 Cluster: Non-specific serine/threonine protein
kinase; n=1; Candidatus Nitrosopumilus maritimus
SCM1|Rep: Non-specific serine/threonine protein kinase -
Candidatus Nitrosopumilus maritimus SCM1
Length = 238
Score = 44.4 bits (100), Expect = 0.002
Identities = 23/48 (47%), Positives = 31/48 (64%)
Frame = +1
Query: 457 LVTKNLVPXSXVYGEXXISLDNERDKVEYXVWNPFXSKLAAAIMGGVD 600
L T+NLVP + VY E I + +EY +W+PF SKLAAAIM ++
Sbjct: 33 LATENLVPGNQVYKEKLII----KKGIEYRLWDPFRSKLAAAIMNELE 76
>UniRef50_UPI00015BB1B1 Cluster: rRNA 2'-O-methyltransferase
fibrillarin; n=1; Ignicoccus hospitalis KIN4/I|Rep: rRNA
2'-O-methyltransferase fibrillarin - Ignicoccus
hospitalis KIN4/I
Length = 239
Score = 42.3 bits (95), Expect = 0.009
Identities = 23/54 (42%), Positives = 29/54 (53%)
Frame = +1
Query: 457 LVTKNLVPXSXVYGEXXISLDNERDKVEYXVWNPFXSKLAAAIMGGVDAIHMAP 618
L TKNL P VYGE E EY WN SKLAAA++ G++ + + P
Sbjct: 29 LATKNLTPGHRVYGEKLFQWQGE----EYREWNVQRSKLAAALVKGLEELPIKP 78
>UniRef50_Q971W2 Cluster: Fibrillarin-like rRNA/tRNA
2'-O-methyltransferase; n=14; Archaea|Rep:
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase -
Sulfolobus tokodaii
Length = 233
Score = 42.3 bits (95), Expect = 0.009
Identities = 22/47 (46%), Positives = 26/47 (55%)
Frame = +1
Query: 457 LVTKNLVPXSXVYGEXXISLDNERDKVEYXVWNPFXSKLAAAIMGGV 597
L TKNL P VYGE ++ VEY WN F SKL AI+ G+
Sbjct: 27 LCTKNLAPGFSVYGERLFKVEG----VEYREWNAFRSKLGGAILKGL 69
>UniRef50_Q58108 Cluster: Fibrillarin-like rRNA/tRNA
2'-O-methyltransferase; n=8; Methanococcales|Rep:
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase -
Methanococcus jannaschii
Length = 230
Score = 41.1 bits (92), Expect = 0.021
Identities = 21/47 (44%), Positives = 29/47 (61%)
Frame = +1
Query: 457 LVTKNLVPXSXVYGEXXISLDNERDKVEYXVWNPFXSKLAAAIMGGV 597
+ TK++V VY E I + +E EY +WNP SKLAAAI+ G+
Sbjct: 25 IATKSIVKGKKVYDEKIIKIGDE----EYRIWNPNKSKLAAAIIKGL 67
>UniRef50_A0RV24 Cluster: Fibrillarin-like rRNA methylase; n=1;
Cenarchaeum symbiosum|Rep: Fibrillarin-like rRNA
methylase - Cenarchaeum symbiosum
Length = 201
Score = 40.7 bits (91), Expect = 0.028
Identities = 20/49 (40%), Positives = 28/49 (57%)
Frame = +1
Query: 478 PXSXVYGEXXISLDNERDKVEYXVWNPFXSKLAAAIMGGVDAIHMAPXS 624
P VYGE + +R EY +W+PF SKLAA I G++ + + P S
Sbjct: 3 PGCSVYGEKLL----DRAGTEYRLWDPFRSKLAACIYNGLERLPIIPGS 47
>UniRef50_Q5A0V8 Cluster: Putative uncharacterized protein; n=1;
Candida albicans|Rep: Putative uncharacterized protein -
Candida albicans (Yeast)
Length = 106
Score = 39.1 bits (87), Expect = 0.085
Identities = 16/25 (64%), Positives = 17/25 (68%)
Frame = -3
Query: 470 FLVTNAXXLPLXMNTPGCLCGSIMT 396
FLVTN LP M TP CLCGS+ T
Sbjct: 2 FLVTNKSSLPSAMKTPACLCGSMTT 26
>UniRef50_O27283 Cluster: Fibrillarin-like rRNA/tRNA
2'-O-methyltransferase; n=4; Methanobacteriaceae|Rep:
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase -
Methanobacterium thermoautotrophicum
Length = 224
Score = 39.1 bits (87), Expect = 0.085
Identities = 26/58 (44%), Positives = 31/58 (53%)
Frame = +1
Query: 424 GVFIXRGKXXALVTKNLVPXSXVYGEXXISLDNERDKVEYXVWNPFXSKLAAAIMGGV 597
GVFI +L+T N P VYGE I EY VW+P SKLAAAI+ G+
Sbjct: 12 GVFIMNN---SLLTINPNPGVKVYGERIIDWGGR----EYRVWDPRRSKLAAAILNGL 62
>UniRef50_Q8TTT4 Cluster: Fibrillarin-like rRNA/tRNA
2'-O-methyltransferase; n=4; Methanosarcinaceae|Rep:
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase -
Methanosarcina acetivorans
Length = 227
Score = 37.9 bits (84), Expect = 0.20
Identities = 21/55 (38%), Positives = 27/55 (49%)
Frame = +1
Query: 424 GVFIXRGKXXALVTKNLVPXSXVYGEXXISLDNERDKVEYXVWNPFXSKLAAAIM 588
G+F L T NL P VYGE IS++ EY W+P SKL A ++
Sbjct: 10 GIFEIMKDKRQLATLNLDPGKVVYGEKLISVEG----AEYRTWDPRRSKLGAMVL 60
>UniRef50_Q1FNR9 Cluster: Cobalamin (Vitamin B12) biosynthesis CbiX
protein:Anaerobic cobalt chelatase; n=2;
Clostridiales|Rep: Cobalamin (Vitamin B12) biosynthesis
CbiX protein:Anaerobic cobalt chelatase - Clostridium
phytofermentans ISDg
Length = 261
Score = 32.3 bits (70), Expect = 9.7
Identities = 12/35 (34%), Positives = 22/35 (62%)
Frame = +1
Query: 508 ISLDNERDKVEYXVWNPFXSKLAAAIMGGVDAIHM 612
I +D +++ +EY ++ F SK+ I+G D IH+
Sbjct: 26 IEMDIQQEYLEYAIYRAFTSKMIINILGSRDGIHV 60
>UniRef50_Q11VL3 Cluster: Putative uncharacterized protein; n=1;
Cytophaga hutchinsonii ATCC 33406|Rep: Putative
uncharacterized protein - Cytophaga hutchinsonii (strain
ATCC 33406 / NCIMB 9469)
Length = 427
Score = 32.3 bits (70), Expect = 9.7
Identities = 10/28 (35%), Positives = 17/28 (60%)
Frame = -2
Query: 597 HSTHNCSCQFRXERIPDXVLHLITFVVK 514
H H+C+C R ++ + H IT+V+K
Sbjct: 56 HHLHSCACPLRQQKAESKISHTITYVIK 83
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 352,766,827
Number of Sequences: 1657284
Number of extensions: 3932414
Number of successful extensions: 7262
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 7185
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 7253
length of database: 575,637,011
effective HSP length: 97
effective length of database: 414,880,463
effective search space used: 45636850930
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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