BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fprWP02_F_G05 (648 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g19770.1 68415.m02310 profilin 4 (PRO4) (PFN4) identical to p... 99 1e-21 At5g56600.1 68418.m07065 profilin 5 (PRO5) (PRF3) identical to S... 96 2e-20 At4g29340.1 68417.m04192 profilin 3 (PRO3) (PFN3) identical to p... 95 3e-20 At4g29350.1 68417.m04193 profilin 2 (PRO2) (PFN2) (PRF2) identic... 93 1e-19 At2g19760.1 68415.m02309 profilin 1 (PRO1) (PFN1) (PRF1) / aller... 91 8e-19 At1g62420.1 68414.m07042 expressed protein contains Pfam profile... 33 0.22 At5g17850.1 68418.m02092 cation exchanger, putative (CAX8) simil... 29 2.7 At2g22950.1 68415.m02725 calcium-transporting ATPase, plasma mem... 28 4.7 At2g41560.1 68415.m05136 calcium-transporting ATPase 4, plasma m... 28 6.1 >At2g19770.1 68415.m02310 profilin 4 (PRO4) (PFN4) identical to profilin 4 SP:Q38905 GI:1353768 from [Arabidopsis thaliana] Length = 134 Score = 99 bits (238), Expect = 1e-21 Identities = 55/133 (41%), Positives = 76/133 (57%), Gaps = 8/133 (6%) Frame = +1 Query: 91 MSWQDYVDKQLMASXC------VTKAAIAGHDGNVWAKSEGF-EISKDEVAKIVAGFENE 249 MSWQ YVD+ LM +T AAI GHDG+VWA+S F + E+ I+ F+ Sbjct: 1 MSWQAYVDEHLMCDVGDGQGHHLTAAAIIGHDGSVWAQSANFPQFKPQEITDIMKDFDEP 60 Query: 250 SLLTSGGVTIAGTRYIYLSGTDH-IIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQAAS 426 L G+ +AG +Y+ + G + +IR K G G+ KT Q++V LYEEP+ P Q Sbjct: 61 GHLAPTGMFLAGLKYMVIQGEPNAVIRGKKGAGGITIKKTGQSMVFGLYEEPVTPGQCNM 120 Query: 427 VVEKLXEYLITCG 465 VVE+L +YLI G Sbjct: 121 VVERLGDYLIEQG 133 >At5g56600.1 68418.m07065 profilin 5 (PRO5) (PRF3) identical to SP|Q9FE63 Profilin 5 {Arabidopsis thaliana} Length = 168 Score = 96.3 bits (229), Expect = 2e-20 Identities = 54/130 (41%), Positives = 72/130 (55%), Gaps = 5/130 (3%) Frame = +1 Query: 91 MSWQDYVDKQLM---ASXCVTKAAIAGHDGNVWAKSEGF-EISKDEVAKIVAGFENESLL 258 MSWQ YVD LM A +T AAI G DG+VWA+S F ++ +E+ I F L Sbjct: 38 MSWQTYVDDHLMCDVAGNRLTAAAILGQDGSVWAQSNNFPQVKPEEIQGIKDDFTTPGTL 97 Query: 259 TSGGVTIAGTRYIYLSGTDH-IIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQAASVVE 435 G+ + G +Y+ + G + +IR K G GV KT A+V +Y+EP+ P Q VVE Sbjct: 98 APTGLFLGGNKYMVIQGEPNAVIRGKKGAGGVTIKKTTLALVFGIYDEPMTPGQCNMVVE 157 Query: 436 KLXEYLITCG 465 L EYLI G Sbjct: 158 NLGEYLIESG 167 >At4g29340.1 68417.m04192 profilin 3 (PRO3) (PFN3) identical to profilin 3 SP:Q38904 GI:1353765 from [Arabidopsis thaliana] Length = 134 Score = 95.5 bits (227), Expect = 3e-20 Identities = 53/133 (39%), Positives = 74/133 (55%), Gaps = 8/133 (6%) Frame = +1 Query: 91 MSWQDYVDKQLMASXC------VTKAAIAGHDGNVWAKSEGF-EISKDEVAKIVAGFENE 249 MSWQ YVD+ LM +T AAI GHDG+VWA+S F + E + I+ F+ Sbjct: 1 MSWQTYVDEHLMCDVGDGQGHHLTAAAIVGHDGSVWAQSANFPQFKGQEFSDIMKDFDEP 60 Query: 250 SLLTSGGVTIAGTRYIYLSGTDH-IIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQAAS 426 L G+ +AG +Y+ + G +IR K G G+ KT Q+ V +YEEP+ P Q Sbjct: 61 GHLAPTGLFMAGAKYMVIQGEPGAVIRGKKGAGGITIKKTGQSCVFGIYEEPVTPGQCNM 120 Query: 427 VVEKLXEYLITCG 465 VVE+L +YL+ G Sbjct: 121 VVERLGDYLLEQG 133 >At4g29350.1 68417.m04193 profilin 2 (PRO2) (PFN2) (PRF2) identical to profilin 2 SP:Q42418 GI:1353772 from [Arabidopsis thaliana]; identical to cDNA profilin (PRF2) GI:9965570 Length = 131 Score = 93.1 bits (221), Expect = 1e-19 Identities = 54/130 (41%), Positives = 72/130 (55%), Gaps = 5/130 (3%) Frame = +1 Query: 91 MSWQDYVDKQLMASX---CVTKAAIAGHDGNVWAKSEGF-EISKDEVAKIVAGFENESLL 258 MSWQ YVD LM +T AAI G DG+VWA+S F ++ E+A I FE L Sbjct: 1 MSWQSYVDDHLMCEVEGNHLTHAAIFGQDGSVWAQSSAFPQLKPAEIAGINKDFEEAGHL 60 Query: 259 TSGGVTIAGTRYIYLSG-TDHIIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQAASVVE 435 G+ + G +Y+ + G +IR K G GV KT QA+V +Y+EP+ Q VVE Sbjct: 61 APTGLFLGGEKYMVVQGEAGAVIRGKKGPGGVTIKKTTQALVFGIYDEPMTGGQCNLVVE 120 Query: 436 KLXEYLITCG 465 +L +YLI G Sbjct: 121 RLGDYLIESG 130 >At2g19760.1 68415.m02309 profilin 1 (PRO1) (PFN1) (PRF1) / allergen Ara t 8 identical to profilin 1 (Allergen Ara t 8) SP:Q42449 GI:1353770 from [Arabidopsis thaliana] Length = 131 Score = 90.6 bits (215), Expect = 8e-19 Identities = 52/127 (40%), Positives = 69/127 (54%), Gaps = 5/127 (3%) Frame = +1 Query: 91 MSWQDYVDKQLMASX---CVTKAAIAGHDGNVWAKSEGF-EISKDEVAKIVAGFENESLL 258 MSWQ YVD LM +T AAI G DG+VWA+S F ++ E+ I FE L Sbjct: 1 MSWQSYVDDHLMCDVEGNHLTAAAILGQDGSVWAQSAKFPQLKPQEIDGIKKDFEEPGFL 60 Query: 259 TSGGVTIAGTRYIYLSGTDH-IIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQAASVVE 435 G+ + G +Y+ + G +IR K G GV KT QA+V Y+EP+ Q VVE Sbjct: 61 APTGLFLGGEKYMVIQGEQGAVIRGKKGPGGVTIKKTNQALVFGFYDEPMTGGQCNLVVE 120 Query: 436 KLXEYLI 456 +L +YLI Sbjct: 121 RLGDYLI 127 >At1g62420.1 68414.m07042 expressed protein contains Pfam profile PF04720: Protein of unknown function (DUF506) Length = 465 Score = 32.7 bits (71), Expect = 0.22 Identities = 16/63 (25%), Positives = 32/63 (50%) Frame = +3 Query: 249 ITANEWRRDDSGHAVHLPQWHRPYHPREAWQGRRALHEDTASCGHFSL*RTHPTSAGRIC 428 I +E RR +H+P W R + + W G ++ T++ ++ + T+ T+A + C Sbjct: 199 IMCHEMRRSMKHVGIHVPPWRRNGYMQAKWFG---FYKRTSTTNNYEMVNTYDTTAFKGC 255 Query: 429 RGE 437 + E Sbjct: 256 KEE 258 >At5g17850.1 68418.m02092 cation exchanger, putative (CAX8) similar to sodium/calcium exchanger protein [Mus musculus] gi|13925661|gb|AAK49407; Ca2+:Cation Antiporter (CaCA) Family member PMID:11500563 Length = 559 Score = 29.1 bits (62), Expect = 2.7 Identities = 13/33 (39%), Positives = 21/33 (63%) Frame = -2 Query: 323 MIWSVPLR*MYRVPAIVTPPLVSSDSFSKPATI 225 +IW++ L +P I+T P+VS D +SKP + Sbjct: 307 VIWAITLP--LNLPRILTIPVVSEDKWSKPLAV 337 >At2g22950.1 68415.m02725 calcium-transporting ATPase, plasma membrane-type, putative / Ca2+-ATPase, putative (ACA7) identical to SP|O64806 Potential calcium-transporting ATPase 7, plasma membrane-type (EC 3.6.3.8) (Ca(2+)-ATPase isoform 7) {Arabidopsis thaliana}; strong similarity to SP|O81108 Calcium-transporting ATPase 2, plasma membrane-type (EC 3.6.3.8) (Ca(2+)-ATPase isoform 2) {Arabidopsis thaliana} Length = 1015 Score = 28.3 bits (60), Expect = 4.7 Identities = 13/30 (43%), Positives = 18/30 (60%) Frame = +1 Query: 184 KSEGFEISKDEVAKIVAGFENESLLTSGGV 273 K+ GF+I DE+ IV G + + L GGV Sbjct: 90 KAAGFDICADELGSIVEGHDVKKLKFHGGV 119 >At2g41560.1 68415.m05136 calcium-transporting ATPase 4, plasma membrane-type / Ca2+-ATPase, isoform 4 (ACA4) identical to SP|O22218 Calcium-transporting ATPase 4, plasma membrane-type (EC 3.6.3.8) (Ca(2+)-ATPase isoform 4) {Arabidopsis thaliana} Length = 1030 Score = 27.9 bits (59), Expect = 6.1 Identities = 13/30 (43%), Positives = 17/30 (56%) Frame = +1 Query: 184 KSEGFEISKDEVAKIVAGFENESLLTSGGV 273 K GF I DE+A +V + +SL GGV Sbjct: 89 KKAGFSIEADELASMVRKNDTKSLAQKGGV 118 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,969,134 Number of Sequences: 28952 Number of extensions: 250345 Number of successful extensions: 566 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 545 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 556 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1344285648 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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