Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fprWP02_F_D15
(652 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) riboso... 227 4e-60
At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B) 227 5e-60
At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contai... 227 5e-60
At4g10710.1 68417.m01751 transcriptional regulator-related simil... 31 0.66
At1g29760.1 68414.m03639 expressed protein 31 0.66
At5g15400.1 68418.m01802 U-box domain-containing protein contain... 30 1.2
At4g38440.1 68417.m05432 expressed protein 29 3.5
At4g08180.3 68417.m01353 oxysterol-binding family protein simila... 28 4.7
At4g08180.2 68417.m01352 oxysterol-binding family protein simila... 28 4.7
At4g08180.1 68417.m01351 oxysterol-binding family protein simila... 28 4.7
At2g38570.1 68415.m04738 expressed protein ; expression supporte... 28 6.2
At4g21370.1 68417.m03088 S-locus protein kinase, putative simila... 27 8.2
At2g26730.1 68415.m03206 leucine-rich repeat transmembrane prote... 27 8.2
>At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) ribosomal
protein S4, Arabidopsis thaliana, PIR:T48480
Length = 262
Score = 227 bits (556), Expect = 4e-60
Identities = 109/185 (58%), Positives = 131/185 (70%)
Frame = +1
Query: 97 PKHGCWTNLAVCMHRDRQPVPTSCASACPWLIFLRNRLKYALTGNEVLKIVKQRLIKVDG 276
PKH L P P ++ +RNRLKYALT EV+ I+ QR I+VDG
Sbjct: 15 PKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREVISILMQRHIQVDG 74
Query: 277 KVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPK 456
KVRTD TYPAGFMDVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G K
Sbjct: 75 KVRTDKTYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQK 134
Query: 457 NVPYLVTHDGRTIRYPDPLIKVNDSIQLDIATTXIMDFIKFESGNLCMITGGRNLGRVGT 636
+PYL T+DGRTIRYPDPLIK ND+I+LD+ I++FIKF+ GN+ M+TGGRN GRVG
Sbjct: 135 GIPYLNTYDGRTIRYPDPLIKPNDTIKLDLEANKIVEFIKFDVGNVVMVTGGRNRGRVGV 194
Query: 637 IVSRE 651
I +RE
Sbjct: 195 IKNRE 199
Score = 87.0 bits (206), Expect = 1e-17
Identities = 37/44 (84%), Positives = 40/44 (90%)
Frame = +3
Query: 54 MARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPL 185
MARG KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPL
Sbjct: 1 MARGLKKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPL 44
>At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B)
Length = 262
Score = 227 bits (555), Expect = 5e-60
Identities = 109/185 (58%), Positives = 131/185 (70%)
Frame = +1
Query: 97 PKHGCWTNLAVCMHRDRQPVPTSCASACPWLIFLRNRLKYALTGNEVLKIVKQRLIKVDG 276
PKH L P P ++ +RNRLKYALT EV+ I+ QR I+VDG
Sbjct: 15 PKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREVISILMQRHIQVDG 74
Query: 277 KVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPK 456
KVRTD TYPAGFMDVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G K
Sbjct: 75 KVRTDKTYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQK 134
Query: 457 NVPYLVTHDGRTIRYPDPLIKVNDSIQLDIATTXIMDFIKFESGNLCMITGGRNLGRVGT 636
+PYL T+DGRTIRYPDPLIK ND+I+LD+ I++FIKF+ GN+ M+TGGRN GRVG
Sbjct: 135 GIPYLNTYDGRTIRYPDPLIKPNDTIKLDLEENKIVEFIKFDVGNVVMVTGGRNRGRVGV 194
Query: 637 IVSRE 651
I +RE
Sbjct: 195 IKNRE 199
Score = 87.0 bits (206), Expect = 1e-17
Identities = 37/44 (84%), Positives = 40/44 (90%)
Frame = +3
Query: 54 MARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPL 185
MARG KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPL
Sbjct: 1 MARGLKKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPL 44
>At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contains
ribosomal protein S4 signature from residues 8 to 22
Length = 261
Score = 227 bits (555), Expect = 5e-60
Identities = 109/185 (58%), Positives = 131/185 (70%)
Frame = +1
Query: 97 PKHGCWTNLAVCMHRDRQPVPTSCASACPWLIFLRNRLKYALTGNEVLKIVKQRLIKVDG 276
PKH L P P ++ +RNRLKYALT EV+ I+ QR I+VDG
Sbjct: 15 PKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREVISILMQRHIQVDG 74
Query: 277 KVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPK 456
KVRTD TYPAGFMDVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G K
Sbjct: 75 KVRTDKTYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQK 134
Query: 457 NVPYLVTHDGRTIRYPDPLIKVNDSIQLDIATTXIMDFIKFESGNLCMITGGRNLGRVGT 636
+PYL T+DGRTIRYPDPLIK ND+I+LD+ I++FIKF+ GN+ M+TGGRN GRVG
Sbjct: 135 GIPYLNTYDGRTIRYPDPLIKPNDTIKLDLEENKIVEFIKFDVGNVVMVTGGRNRGRVGV 194
Query: 637 IVSRE 651
I +RE
Sbjct: 195 IKNRE 199
Score = 87.0 bits (206), Expect = 1e-17
Identities = 37/44 (84%), Positives = 40/44 (90%)
Frame = +3
Query: 54 MARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPL 185
MARG KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPL
Sbjct: 1 MARGLKKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPL 44
>At4g10710.1 68417.m01751 transcriptional regulator-related similar
to chromatin-specific transcription elongation factor
FACT 140 kDa subunit (GI:5499741) [Homo sapiens]
Length = 1074
Score = 31.1 bits (67), Expect = 0.66
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = -1
Query: 178 KHSRNLWGPVDGLGAYTPPSLSNIHAL 98
KHS +LWG D L TPP+ ++ L
Sbjct: 44 KHSADLWGSADALAIATPPASDDLRYL 70
>At1g29760.1 68414.m03639 expressed protein
Length = 526
Score = 31.1 bits (67), Expect = 0.66
Identities = 14/31 (45%), Positives = 17/31 (54%)
Frame = -3
Query: 290 SVLTFPSTFMRRCFTIFRTSFPVKAYFRRFL 198
S+LTFP +R CF F F + RRFL
Sbjct: 195 SLLTFPPWLLRNCFLFFFDPFSTIRFGRRFL 225
>At5g15400.1 68418.m01802 U-box domain-containing protein contains
Pfam profile PF04564: U-box domain
Length = 1038
Score = 30.3 bits (65), Expect = 1.2
Identities = 16/52 (30%), Positives = 26/52 (50%)
Frame = +3
Query: 105 WMLDKLGGVYAPRPSTGPHKLRECLPLADFPEESSEVCFDRKRSPENCETTP 260
W++ +GG P PST P + C+P F E++ E+ R P+ + P
Sbjct: 566 WLVGLVGGFKMPLPSTCPMEF-SCMP-EHFVEDAMELLIFASRIPKALDGVP 615
>At4g38440.1 68417.m05432 expressed protein
Length = 1465
Score = 28.7 bits (61), Expect = 3.5
Identities = 12/36 (33%), Positives = 20/36 (55%)
Frame = +3
Query: 81 KRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLA 188
+R+ P W L + V++ + STGP + E L +A
Sbjct: 1140 QRMPLPPHWFLSAISAVHSGKTSTGPPESTELLEVA 1175
>At4g08180.3 68417.m01353 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 813
Score = 28.3 bits (60), Expect = 4.7
Identities = 15/46 (32%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
Frame = +1
Query: 202 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY-PAGFMDVVSIEK 336
N ++A+T NE+ +K+RL D ++R D Y G ++ + EK
Sbjct: 703 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRYLENGEFEMANTEK 748
>At4g08180.2 68417.m01352 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 813
Score = 28.3 bits (60), Expect = 4.7
Identities = 15/46 (32%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
Frame = +1
Query: 202 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY-PAGFMDVVSIEK 336
N ++A+T NE+ +K+RL D ++R D Y G ++ + EK
Sbjct: 703 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRYLENGEFEMANTEK 748
>At4g08180.1 68417.m01351 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 814
Score = 28.3 bits (60), Expect = 4.7
Identities = 15/46 (32%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
Frame = +1
Query: 202 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY-PAGFMDVVSIEK 336
N ++A+T NE+ +K+RL D ++R D Y G ++ + EK
Sbjct: 704 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRYLENGEFEMANTEK 749
>At2g38570.1 68415.m04738 expressed protein ; expression supported
by MPSS
Length = 302
Score = 27.9 bits (59), Expect = 6.2
Identities = 12/28 (42%), Positives = 17/28 (60%)
Frame = +1
Query: 553 TXIMDFIKFESGNLCMITGGRNLGRVGT 636
T I+ +IK G + GGRN+G+V T
Sbjct: 196 TYILVYIKISVGYRVVTPGGRNIGKVST 223
>At4g21370.1 68417.m03088 S-locus protein kinase, putative similar
to SRKa [Arabidopsis lyrata] gi|13620927|dbj|BAB40986;
contains Pfam domains PF00954: S-locus glycoprotein
family, PF00069: Protein kinase domain and PF01453:
Lectin (probable mannose binding)
Length = 844
Score = 27.5 bits (58), Expect = 8.2
Identities = 11/23 (47%), Positives = 14/23 (60%)
Frame = -1
Query: 226 RSKHTSDDSSGKSARGKHSRNLW 158
RS +DDSS + R H R+LW
Sbjct: 813 RSSLDTDDSSSSTERDNHRRHLW 835
>At2g26730.1 68415.m03206 leucine-rich repeat transmembrane protein
kinase, putative
Length = 658
Score = 27.5 bits (58), Expect = 8.2
Identities = 13/44 (29%), Positives = 23/44 (52%)
Frame = +1
Query: 478 HDGRTIRYPDPLIKVNDSIQLDIATTXIMDFIKFESGNLCMITG 609
H+ + +P ++N+ I+LDI++ I F NL +TG
Sbjct: 124 HNEFSGEFPTSFTQLNNLIRLDISSNNFTGSIPFSVNNLTHLTG 167
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,740,489
Number of Sequences: 28952
Number of extensions: 319604
Number of successful extensions: 861
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 822
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 861
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1354097952
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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