Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fprWP02_F_D14
(656 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein (H... 42 3e-04
At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote... 39 0.003
At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HS... 39 0.003
At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS... 37 0.014
At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote... 34 0.072
At4g21870.1 68417.m03163 26.5 kDa class P-related heat shock pro... 31 0.67
At2g20360.1 68415.m02377 expressed protein 29 2.7
At5g18190.1 68418.m02135 protein kinase family protein contains ... 28 4.8
At1g73710.1 68414.m08535 pentatricopeptide (PPR) repeat-containi... 28 4.8
At5g55670.1 68418.m06941 RNA recognition motif (RRM)-containing ... 28 6.3
At4g36850.1 68417.m05225 PQ-loop repeat family protein / transme... 27 8.3
At1g65710.1 68414.m07458 expressed protein ; expression supporte... 27 8.3
At1g26140.1 68414.m03191 hypothetical protein 27 8.3
>At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein
(HSP22.0-ER) identical to endomembrane-localized small
heat shock protein GI:511795 from [Arabidopsis thaliana]
Length = 195
Score = 42.3 bits (95), Expect = 3e-04
Identities = 32/104 (30%), Positives = 55/104 (52%), Gaps = 8/104 (7%)
Frame = +2
Query: 299 KADKDKFQVNLDVQHFSPEEISVKTAD-GYIVVXG--KHEEXK--DEHGYISR---QFVR 454
K + ++ LD+ +E+ ++ + G + V G K EE K D+ + R +F R
Sbjct: 76 KETAEGHEIMLDIPGLKKDEVKIEVEENGVLRVSGERKREEEKKGDQWHRVERSYGKFWR 135
Query: 455 RYALPEGAAPXTVESRLSSDGVLTITAPRKVPDAVKGERKVPIA 586
++ LP+ +V+++L + GVLTI + P+ VKG R V IA
Sbjct: 136 QFKLPDNVDMESVKAKLEN-GVLTINLTKLSPEKVKGPRVVNIA 178
>At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein
(HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I
heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853)
(GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res.
17 (19), 7995 (1989))
Length = 157
Score = 39.1 bits (87), Expect = 0.003
Identities = 28/93 (30%), Positives = 50/93 (53%), Gaps = 8/93 (8%)
Frame = +2
Query: 332 DVQHFSPEEISVKTADGYIV-VXGK----HEEXKDEHGYISR---QFVRRYALPEGAAPX 487
D+ EE+ V+ DG I+ + G+ +EE D+ + R +F RR+ LPE A
Sbjct: 66 DLPGLRKEEVKVEVEDGNILQISGERSNENEEKNDKWHRVERSSGKFTRRFRLPENAKME 125
Query: 488 TVESRLSSDGVLTITAPRKVPDAVKGERKVPIA 586
+++ + +GVL++T P KVP+ + + I+
Sbjct: 126 EIKASM-ENGVLSVTVP-KVPEKKPEVKSIDIS 156
>At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein
(HSP17.4-CI) identical to 17.4 kDa class I heat shock
protein SP:P19036 from [Arabidopsis thaliana]
Length = 156
Score = 38.7 bits (86), Expect = 0.003
Identities = 30/93 (32%), Positives = 50/93 (53%), Gaps = 8/93 (8%)
Frame = +2
Query: 332 DVQHFSPEEISVKTADGYIV-VXGK----HEEXKDEHGYISR---QFVRRYALPEGAAPX 487
DV EE+ V+ DG I+ + G+ +EE D + R +F+RR+ LPE A
Sbjct: 65 DVPGLKKEEVKVEVEDGNILQISGERSSENEEKSDTWHRVERSSGKFMRRFRLPENAKVE 124
Query: 488 TVESRLSSDGVLTITAPRKVPDAVKGERKVPIA 586
V++ + +GVL++T P KV ++ + V I+
Sbjct: 125 EVKASM-ENGVLSVTVP-KVQESKPEVKSVDIS 155
>At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein
(HSP18.1-CI) identical to 18.2 kDa class I heat shock
protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana];
contains Pfam profile: PF00011 Hsp20/alpha crystallin
family
Length = 161
Score = 36.7 bits (81), Expect = 0.014
Identities = 37/133 (27%), Positives = 63/133 (47%), Gaps = 16/133 (12%)
Frame = +2
Query: 236 EDYFRPWRQLAAAS--RDLGSSIKADKDKFQV------NLDVQHFSPEEISVKTADGYIV 391
E +F P LA AS RD+ + A D + D+ EE+ V+ D ++
Sbjct: 28 EGFFTPSSALANASTARDVAAFTNARVDWKETPEAHVFKADLPGLKKEEVKVEVEDKNVL 87
Query: 392 -VXGK----HEEXKDEHGYISR---QFVRRYALPEGAAPXTVESRLSSDGVLTITAPRKV 547
+ G+ +EE D+ + R +F+RR+ LPE A V++ + +GVLT+ P K
Sbjct: 88 QISGERSKENEEKNDKWHRVERASGKFMRRFRLPENAKMEEVKATM-ENGVLTVVVP-KA 145
Query: 548 PDAVKGERKVPIA 586
P+ + + I+
Sbjct: 146 PEKKPQVKSIDIS 158
>At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein
(HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha
crystallin family; identified in Scharf, K-D., et al,
Cell Stress & Chaperones (2001) 6: 225-237.
Length = 153
Score = 34.3 bits (75), Expect = 0.072
Identities = 24/78 (30%), Positives = 40/78 (51%), Gaps = 8/78 (10%)
Frame = +2
Query: 332 DVQHFSPEEISVKTA-DGYIVVXG-KHEEXKDEHGYISR------QFVRRYALPEGAAPX 487
D+ EE+ V+ D + + G +H E +D++ R QF RR+ LPE
Sbjct: 62 DLPGLKKEEVKVEIEEDSVLKISGERHVEKEDKNDTWHRVERSSGQFTRRFRLPENVKMD 121
Query: 488 TVESRLSSDGVLTITAPR 541
V++ + +GVLT+T P+
Sbjct: 122 QVKAAM-ENGVLTVTVPK 138
>At4g21870.1 68417.m03163 26.5 kDa class P-related heat shock
protein (HSP26.5-P) contains Pfam profile: PF00011
Hsp20/alpha crystallin family: identified in Scharf,
K-D., et al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 134
Score = 31.1 bits (67), Expect = 0.67
Identities = 18/79 (22%), Positives = 33/79 (41%)
Frame = +2
Query: 311 DKFQVNLDVQHFSPEEISVKTADGYIVVXGKHEEXKDEHGYISRQFVRRYALPEGAAPXT 490
D ++D+ EEI V+ D ++ + F R++ LPE
Sbjct: 35 DSHTFSVDLPGLRKEEIKVEIEDSIYLIIRTEATPMSPPDQPLKTFKRKFRLPESIDMIG 94
Query: 491 VESRLSSDGVLTITAPRKV 547
+ + DGVLT+ P+++
Sbjct: 95 ISAGYE-DGVLTVIVPKRI 112
>At2g20360.1 68415.m02377 expressed protein
Length = 402
Score = 29.1 bits (62), Expect = 2.7
Identities = 13/26 (50%), Positives = 16/26 (61%)
Frame = -2
Query: 601 NGTGXCDGHLSLSLDGVGYLPRRGDG 524
NGT C SL+ GVG+L R+G G
Sbjct: 36 NGTDNCRYSSSLATKGVGHLARKGTG 61
>At5g18190.1 68418.m02135 protein kinase family protein contains
Pfam domains, PF00069: Protein kinase domain
Length = 691
Score = 28.3 bits (60), Expect = 4.8
Identities = 11/22 (50%), Positives = 12/22 (54%)
Frame = +1
Query: 247 QTVETTRGCIS*PRLQHKGRQG 312
Q T GC P + HKGRQG
Sbjct: 185 QVYNTLNGCYGVPAVHHKGRQG 206
>At1g73710.1 68414.m08535 pentatricopeptide (PPR) repeat-containing
protein low similarity to fertility restorer [Petunia x
hybrida] GI:22128587, post-transcriptional control of
chloroplast gene expression CRP1 [Zea mays] GI:3289002;
contains Pfam profile PF01535: PPR repeat
Length = 991
Score = 28.3 bits (60), Expect = 4.8
Identities = 10/27 (37%), Positives = 15/27 (55%)
Frame = -2
Query: 592 GXCDGHLSLSLDGVGYLPRRGDGENPV 512
G C G + L LD + P+ G ++PV
Sbjct: 240 GWCAGKVDLDLDSIDDFPKNGSAQSPV 266
>At5g55670.1 68418.m06941 RNA recognition motif (RRM)-containing
protein
Length = 710
Score = 27.9 bits (59), Expect = 6.3
Identities = 15/39 (38%), Positives = 19/39 (48%), Gaps = 5/39 (12%)
Frame = +1
Query: 511 RRGSHHHRAEEGTR-----RRQGREKGAHRTXRXRSQGD 612
R HHH+ E +R R + RE+ HR R R GD
Sbjct: 619 REREHHHKDRERSREHVRDRERERERDRHREERERYGGD 657
>At4g36850.1 68417.m05225 PQ-loop repeat family protein /
transmembrane family protein similar to SP|Q10482 Seven
transmembrane protein 1 {Schizosaccharomyces pombe};
contains Pfam profile PF04193: PQ loop repeat
Length = 374
Score = 27.5 bits (58), Expect = 8.3
Identities = 12/50 (24%), Positives = 25/50 (50%)
Frame = -3
Query: 651 LFSASWVPSLWSXISLRTXPVCAMGTFLSPLTASGTFLGAVMVRTPSDDS 502
++ +P +W I + F+ L A+ T++G+++VRT D+
Sbjct: 269 IYMGGRIPQIWLNIKRGSVEGLNPLMFIFALVANATYVGSILVRTTEWDN 318
>At1g65710.1 68414.m07458 expressed protein ; expression supported
by MPSS
Length = 455
Score = 27.5 bits (58), Expect = 8.3
Identities = 16/30 (53%), Positives = 18/30 (60%)
Frame = +3
Query: 495 NRGCHQTGFSPSPRRGRYPTPSRERERCPS 584
+RG + SP RR R TPSRERER S
Sbjct: 155 HRGVERVHGSPRERRRR--TPSRERERSGS 182
>At1g26140.1 68414.m03191 hypothetical protein
Length = 162
Score = 27.5 bits (58), Expect = 8.3
Identities = 10/21 (47%), Positives = 13/21 (61%)
Frame = -2
Query: 274 CSRELSPRSEVVFGEHWTGDG 212
C + L P E V EHW+G+G
Sbjct: 94 CGKTLQPTMEDVKEEHWSGNG 114
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,575,146
Number of Sequences: 28952
Number of extensions: 276506
Number of successful extensions: 898
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 853
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 894
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1373722560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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