BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fprWP02_F_A20 (626 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 200 1e-52 SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 200 1e-52 SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 149 2e-37 SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 89 4e-19 SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 77 2e-15 SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 76 4e-15 SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 69 6e-13 SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 41 2e-04 SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 34 0.019 SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 34 0.019 SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 34 0.019 SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosacch... 29 0.42 SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 28 1.3 SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein |S... 28 1.3 SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 27 2.9 SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr 2|... 26 3.9 SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schi... 26 5.1 SPAP14E8.02 |||transcription factor |Schizosaccharomyces pombe|c... 25 6.8 SPCC965.10 |||transcription factor |Schizosaccharomyces pombe|ch... 25 9.0 >SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 842 Score = 200 bits (488), Expect = 1e-52 Identities = 101/161 (62%), Positives = 123/161 (76%), Gaps = 1/161 (0%) Frame = +1 Query: 97 DEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDR 276 +E+R +M K N+RNMSVIAHVDHGKSTLTDSLV KAGII+ A+AG+ RF DTR DEQ+R Sbjct: 7 EEVRNLMGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGIISAAKAGDARFMDTRADEQER 66 Query: 277 CITIKSTAISMFFELEXKDLVFITNPDQREKSE-KGFLINLIDSPGHVDFSSEVTAALRV 453 +TIKSTAIS+F E+ D+ D +E ++ FL+NLIDSPGHVDFSSEVTAALRV Sbjct: 67 GVTIKSTAISLFAEMTDDDM-----KDMKEPADGTDFLVNLIDSPGHVDFSSEVTAALRV 121 Query: 454 TDGALXXXXXXXXXXXQTETVLRQAIAERIKPILFMNKMDR 576 TDGAL QTETVLRQA+ ERI+P++ +NK+DR Sbjct: 122 TDGALVVVDTIEGVCVQTETVLRQALGERIRPVVVVNKVDR 162 >SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elongation factor 2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 842 Score = 200 bits (488), Expect = 1e-52 Identities = 101/161 (62%), Positives = 123/161 (76%), Gaps = 1/161 (0%) Frame = +1 Query: 97 DEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDR 276 +E+R +M K N+RNMSVIAHVDHGKSTLTDSLV KAGII+ A+AG+ RF DTR DEQ+R Sbjct: 7 EEVRNLMGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGIISAAKAGDARFMDTRADEQER 66 Query: 277 CITIKSTAISMFFELEXKDLVFITNPDQREKSE-KGFLINLIDSPGHVDFSSEVTAALRV 453 +TIKSTAIS+F E+ D+ D +E ++ FL+NLIDSPGHVDFSSEVTAALRV Sbjct: 67 GVTIKSTAISLFAEMTDDDM-----KDMKEPADGTDFLVNLIDSPGHVDFSSEVTAALRV 121 Query: 454 TDGALXXXXXXXXXXXQTETVLRQAIAERIKPILFMNKMDR 576 TDGAL QTETVLRQA+ ERI+P++ +NK+DR Sbjct: 122 TDGALVVVDTIEGVCVQTETVLRQALGERIRPVVVVNKVDR 162 >SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 1000 Score = 149 bits (362), Expect = 2e-37 Identities = 81/160 (50%), Positives = 109/160 (68%) Frame = +1 Query: 97 DEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDR 276 +++ + + NIRN +++AHVDHGK+TL DSL++ GII+ AG RF D R+DE R Sbjct: 7 EKLVSLQKNQENIRNFTLLAHVDHGKTTLADSLLASNGIISSKLAGTVRFLDFREDEITR 66 Query: 277 CITIKSTAISMFFELEXKDLVFITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVT 456 IT+KS+AIS+FF++ I+ D++ + EK +LINLIDSPGHVDFSSEV++A R+ Sbjct: 67 GITMKSSAISLFFKV-------ISQNDEK-RVEKDYLINLIDSPGHVDFSSEVSSASRLC 118 Query: 457 DGALXXXXXXXXXXXQTETVLRQAIAERIKPILFMNKMDR 576 DGA QT TVLRQA +RIK IL +NKMDR Sbjct: 119 DGAFVLVDAVEGVCSQTITVLRQAWIDRIKVILVINKMDR 158 >SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 770 Score = 89.4 bits (212), Expect = 4e-19 Identities = 60/160 (37%), Positives = 84/160 (52%), Gaps = 7/160 (4%) Frame = +1 Query: 118 DKKR--NIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARA--GETRF---TDTRKDEQDR 276 DKKR IRN+ + AH+D GK+T T+ ++ G I G+ D + E+++ Sbjct: 52 DKKRLKQIRNIGISAHIDSGKTTFTERVLYYTGRIKDIHEVRGKDNVGAKMDFMELEREK 111 Query: 277 CITIKSTAISMFFELEXKDLVFITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVT 456 ITI+S A +E + N Q+ EK + IN+ID+PGH+DF+ EV ALRV Sbjct: 112 GITIQSAATHCTWERTVDQIE--ANEKQKTDFEKSYNINIIDTPGHIDFTIEVERALRVL 169 Query: 457 DGALXXXXXXXXXXXQTETVLRQAIAERIKPILFMNKMDR 576 DGA+ QT TV RQ + I F+NKMDR Sbjct: 170 DGAVLVLCAVSGVQSQTITVDRQMRRYNVPRISFVNKMDR 209 >SPBC660.10 |||translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 813 Score = 77.4 bits (182), Expect = 2e-15 Identities = 55/151 (36%), Positives = 78/151 (51%), Gaps = 2/151 (1%) Frame = +1 Query: 130 NIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFT--DTRKDEQDRCITIKSTAI 303 +IRN+ +IAH+D GK+TLT+ ++ G + +T T D E+ R ITI S AI Sbjct: 27 SIRNVGIIAHIDAGKTTLTEKMLYYGGFTSHFGNVDTGDTVMDYLPAERQRGITINSAAI 86 Query: 304 SMFFELEXKDLVFITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDGALXXXXX 483 S + +QR INLID+PGH DF+ EV ++ V DGA+ Sbjct: 87 SFTWR------------NQR--------INLIDTPGHADFTFEVERSVAVLDGAVAIIDG 126 Query: 484 XXXXXXQTETVLRQAIAERIKPILFMNKMDR 576 QT+ V +QA I ++F+NKMDR Sbjct: 127 SAGVEAQTKVVWKQATKRGIPKVIFVNKMDR 157 >SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 646 Score = 76.2 bits (179), Expect = 4e-15 Identities = 49/157 (31%), Positives = 79/157 (50%) Frame = +1 Query: 103 IRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCI 282 +RG+ + +RN +VIAH+DHGKSTL+D ++ G+I +F D + E+ R I Sbjct: 50 VRGIPQNR--VRNWAVIAHIDHGKSTLSDCILKLTGVI-NEHNFRNQFLDKLEVERRRGI 106 Query: 283 TIKSTAISMFFELEXKDLVFITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDG 462 T+K+ SM + + +L+NLID+PGHVDF +EV +L +G Sbjct: 107 TVKAQTCSMIYYYHG----------------QSYLLNLIDTPGHVDFRAEVMHSLAACEG 150 Query: 463 ALXXXXXXXXXXXQTETVLRQAIAERIKPILFMNKMD 573 + QT + A ++ + I +NK+D Sbjct: 151 CILLVDASQGIQAQTLSNFYMAFSQNLVIIPVLNKVD 187 >SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces pombe|chr 2|||Manual Length = 983 Score = 68.9 bits (161), Expect = 6e-13 Identities = 46/157 (29%), Positives = 75/157 (47%), Gaps = 1/157 (0%) Frame = +1 Query: 109 GMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGI-IAGARAGETRFTDTRKDEQDRCIT 285 G++ ++R+ V H+ HGKS L D LV + R+TDT E++R ++ Sbjct: 132 GLLTGTDDVRSFIVAGHLHHGKSALLDLLVYYTHPDTKPPKRRSLRYTDTHYLERERVMS 191 Query: 286 IKSTAISMFFELEXKDLVFITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDGA 465 IKST +++ D + K+ F ID+PGHVDF EV A + ++DG Sbjct: 192 IKSTPLTLAVS------------DMKGKT---FAFQCIDTPGHVDFVDEVAAPMAISDGV 236 Query: 466 LXXXXXXXXXXXQTETVLRQAIAERIKPILFMNKMDR 576 + T +++ AI + +L +NK+DR Sbjct: 237 VLVVDVIEGVMINTTRIIKHAILHDMPIVLVLNKVDR 273 >SPBC9B6.04c |tuf1||mitochondrial translation elongation factor EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 439 Score = 40.7 bits (91), Expect = 2e-04 Identities = 39/152 (25%), Positives = 63/152 (41%), Gaps = 1/152 (0%) Frame = +1 Query: 121 KKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCITIKSTA 300 KK ++ N+ I HVDHGK+TLT ++ + A + D +E+ R ITI S Sbjct: 50 KKPHV-NIGTIGHVDHGKTTLTAAITKCLSDLGQASFMDYSQIDKAPEEKARGITISSAH 108 Query: 301 ISMFFELEXKDLVFITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDGALXXXX 480 + + E + + + +D PGH D+ + DGA+ Sbjct: 109 V------------------EYETANRHYA--HVDCPGHADYIKNMITGAATMDGAIIVVS 148 Query: 481 XXXXXXXQTETVLRQAIAERIKPI-LFMNKMD 573 QT L A +K I +++NK+D Sbjct: 149 ATDGQMPQTREHLLLARQVGVKQIVVYINKVD 180 >SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual Length = 460 Score = 33.9 bits (74), Expect = 0.019 Identities = 16/32 (50%), Positives = 20/32 (62%) Frame = +1 Query: 115 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAG 210 M K++ N+ VI HVD GKST T L+ K G Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCG 32 >SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual Length = 460 Score = 33.9 bits (74), Expect = 0.019 Identities = 16/32 (50%), Positives = 20/32 (62%) Frame = +1 Query: 115 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAG 210 M K++ N+ VI HVD GKST T L+ K G Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCG 32 >SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual Length = 460 Score = 33.9 bits (74), Expect = 0.019 Identities = 16/32 (50%), Positives = 20/32 (62%) Frame = +1 Query: 115 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAG 210 M K++ N+ VI HVD GKST T L+ K G Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCG 32 >SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosaccharomyces pombe|chr 2|||Manual Length = 686 Score = 29.5 bits (63), Expect = 0.42 Identities = 23/67 (34%), Positives = 34/67 (50%), Gaps = 4/67 (5%) Frame = +1 Query: 142 MSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETR----FTDTRKDEQDRCITIKSTAISM 309 ++++ HVDHGK+TL D+ K+ I + G T+ FT D+ + IT T M Sbjct: 174 VTLMGHVDHGKTTLLDAF-RKSTIASTEHGGITQKIGAFT-VPFDKGSKFITFLDTPGHM 231 Query: 310 FFELEXK 330 FE K Sbjct: 232 AFEAMRK 238 >SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma subunit|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 27.9 bits (59), Expect = 1.3 Identities = 13/37 (35%), Positives = 20/37 (54%) Frame = +1 Query: 85 ISRLDEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSL 195 IS L I + ++ N+ I HV HGKST+ ++ Sbjct: 7 ISELSPIHPAIISRQATINIGTIGHVAHGKSTVVKAI 43 >SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 971 Score = 27.9 bits (59), Expect = 1.3 Identities = 17/45 (37%), Positives = 23/45 (51%) Frame = +2 Query: 476 LTVCLVCVYKLKQYCVRLLPSASSLFCS*TKWTVLFLSSNLKLKN 610 L V + YKL+ RL +A S+ C WT LF SN+ +N Sbjct: 348 LNVIGIAAYKLEDPVHRLFVTAFSVCCECLAWTSLF--SNISPEN 390 >SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 662 Score = 26.6 bits (56), Expect = 2.9 Identities = 28/119 (23%), Positives = 51/119 (42%), Gaps = 15/119 (12%) Frame = +1 Query: 139 NMSVIAHVDHGKSTLTDSLVSKAGII----------AGARAGETRF-----TDTRKDEQD 273 N+ I HVD GKSTL +++ G++ AG+ + D+ +E++ Sbjct: 240 NIVFIGHVDAGKSTLGGNILFLTGMVDKRTMEKIEREAKEAGKESWYLSWALDSTSEERE 299 Query: 274 RCITIKSTAISMFFELEXKDLVFITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALR 450 + T++ +FE E + + P KG++ N+I+ D V +A R Sbjct: 300 KGKTVE--VGRAYFETEHRRFSLLDAP-----GHKGYVTNMINGASQADIGVLVISARR 351 >SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr 2|||Manual Length = 207 Score = 26.2 bits (55), Expect = 3.9 Identities = 18/60 (30%), Positives = 29/60 (48%), Gaps = 1/60 (1%) Frame = -1 Query: 428 EEKSTCPGESIKLIKKPFSLFSRWSGFVMNTKSFXS-SSKNIEMAVDLMVMQRSCSSLRV 252 EE + P + K KK S F+ T S S++NI+ A+DL+ + S S ++ Sbjct: 66 EEMESLPSKGGKGSKKAAKKNSSLDAFLNETPQTASYSARNIDDALDLLSLNNSSSKDKI 125 >SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 374 Score = 25.8 bits (54), Expect = 5.1 Identities = 20/76 (26%), Positives = 35/76 (46%), Gaps = 1/76 (1%) Frame = -1 Query: 518 STVSVCTHTPDTQSTT-TRAPSVTRSAAVTSEEKSTCPGESIKLIKKPFSLFSRWSGFVM 342 +T S + +P + STT T +PS + S++ +S S+ S S S S Sbjct: 135 TTSSSSSSSPSSSSTTTTTSPSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSS 194 Query: 341 NTKSFXSSSKNIEMAV 294 ++ S SSS + + + Sbjct: 195 SSSSSSSSSSSSSVPI 210 Score = 25.0 bits (52), Expect = 9.0 Identities = 18/71 (25%), Positives = 32/71 (45%) Frame = -1 Query: 521 RSTVSVCTHTPDTQSTTTRAPSVTRSAAVTSEEKSTCPGESIKLIKKPFSLFSRWSGFVM 342 ++TVS + + T S+++ +PS + + TS S+ S S S S Sbjct: 124 QTTVSSSSVSSTTSSSSSSSPSSSSTTTTTSPSSSSSSSSSSSSSSSSSSSSSSSSSSSS 183 Query: 341 NTKSFXSSSKN 309 ++ S SSS + Sbjct: 184 SSSSSSSSSSS 194 >SPAP14E8.02 |||transcription factor |Schizosaccharomyces pombe|chr 1|||Manual Length = 566 Score = 25.4 bits (53), Expect = 6.8 Identities = 13/37 (35%), Positives = 21/37 (56%), Gaps = 1/37 (2%) Frame = +2 Query: 164 ITASQPSRTRWFPR-PVSLLVREPERPVSLTRVRTNK 271 +T S T + P P S + REP P+S R+R+++ Sbjct: 48 LTPEPSSNTFYAPSSPASAVRREPLSPMSFVRMRSHR 84 >SPCC965.10 |||transcription factor |Schizosaccharomyces pombe|chr 3|||Manual Length = 525 Score = 25.0 bits (52), Expect = 9.0 Identities = 15/37 (40%), Positives = 20/37 (54%) Frame = +1 Query: 247 TDTRKDEQDRCITIKSTAISMFFELEXKDLVFITNPD 357 T RK Q+ I I+ A FEL+ D + +TNPD Sbjct: 61 TKWRKAMQNNSIPIQDLADD--FELDFPDTLDLTNPD 95 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,356,383 Number of Sequences: 5004 Number of extensions: 44299 Number of successful extensions: 159 Number of sequences better than 10.0: 19 Number of HSP's better than 10.0 without gapping: 145 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 155 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 277683324 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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