BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fprWP01_F_P06 (651 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC19F8.03c |||clathrin binding protein|Schizosaccharomyces pom... 30 0.33 SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like pro... 28 1.3 SPAC144.13c |srw1|ste9|CDK inhibitor Srw1|Schizosaccharomyces po... 26 4.1 SPAC16.02c |srp2||mRNA export factor Srp2|Schizosaccharomyces po... 26 4.1 SPAC6G9.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr ... 26 5.4 SPCC18.06c |caf1|pop2|CCR4-Not complex subunit Caf1|Schizosaccha... 25 7.2 SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharom... 25 7.2 SPBC29A10.10c |||tRNA-splicing endonuclease positive effector |S... 25 9.5 SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyce... 25 9.5 SPAC823.12 |||zinc finger protein Pep5/Vps11 |Schizosaccharomyce... 25 9.5 SPBC4C3.06 |||actin cytoskeletal protein Syp1|Schizosaccharomyce... 25 9.5 >SPBC19F8.03c |||clathrin binding protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 649 Score = 29.9 bits (64), Expect = 0.33 Identities = 18/67 (26%), Positives = 32/67 (47%) Frame = +3 Query: 270 ENFKAFCEIESGFPAKDITLHFNGKPLLNNKKSLKEHGVHDGDVIILLHMVNSAQNLSVN 449 EN K + + +SG P ++ + N KP L KKS+ + I + V + S Sbjct: 275 ENRKQYLQNKSGSPVEETAI-LNRKPTLRKKKSIPKKQNESSSTIQKENTVQQEASSSEE 333 Query: 450 DSSQALP 470 ++ ++LP Sbjct: 334 EAVKSLP 340 >SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like protein modifier Ned8|Schizosaccharomyces pombe|chr 2|||Manual Length = 78 Score = 27.9 bits (59), Expect = 1.3 Identities = 18/71 (25%), Positives = 32/71 (45%) Frame = +3 Query: 201 MKVTVTTLNDDIFVLDVSEDLELENFKAFCEIESGFPAKDITLHFNGKPLLNNKKSLKEH 380 M + V TL LD+ + ++ K E + G P L + GK + ++K + H Sbjct: 1 MLIKVKTLTGKEIELDIDPNDKVSRIKERVEEKEGIPPSQQRLIYAGKQMADDKNAESYH 60 Query: 381 GVHDGDVIILL 413 + G V+ L+ Sbjct: 61 -LEGGSVLHLV 70 >SPAC144.13c |srw1|ste9|CDK inhibitor Srw1|Schizosaccharomyces pombe|chr 1|||Manual Length = 556 Score = 26.2 bits (55), Expect = 4.1 Identities = 13/47 (27%), Positives = 24/47 (51%) Frame = +3 Query: 435 NLSVNDSSQALPSGIASLDFSNIQVPTTSANTSMASRNTPVEEDPRI 575 N ++ S+ SGI ++ + P++S +S +NTPV +I Sbjct: 136 NTTIGVSTPRTDSGIDDIELTQRTPPSSSHTSSSILQNTPVTPSRKI 182 >SPAC16.02c |srp2||mRNA export factor Srp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 365 Score = 26.2 bits (55), Expect = 4.1 Identities = 12/35 (34%), Positives = 18/35 (51%) Frame = +3 Query: 258 DLELENFKAFCEIESGFPAKDITLHFNGKPLLNNK 362 D +L N F E+E A+DI F GK + ++ Sbjct: 32 DCKLMNGFGFVEVEDARDARDIVNDFQGKEFMGSR 66 >SPAC6G9.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 681 Score = 25.8 bits (54), Expect = 5.4 Identities = 16/46 (34%), Positives = 26/46 (56%) Frame = +3 Query: 402 IILLHMVNSAQNLSVNDSSQALPSGIASLDFSNIQVPTTSANTSMA 539 I L + +S+++ N S A+ SG+AS F N + + NTSM+ Sbjct: 49 IALSEIPSSSKSGKTNGSKAAVESGLASGAFGN-RTGNMNRNTSMS 93 >SPCC18.06c |caf1|pop2|CCR4-Not complex subunit Caf1|Schizosaccharomyces pombe|chr 3|||Manual Length = 332 Score = 25.4 bits (53), Expect = 7.2 Identities = 19/60 (31%), Positives = 26/60 (43%), Gaps = 2/60 (3%) Frame = +3 Query: 405 ILLHMVNSAQNLSVNDSSQALPSGIASLDFSNIQVPTTSANTSMASR--NTPVEEDPRII 578 +L H +S + D A PS S N +PTT NTS A N+ PR++ Sbjct: 277 VLWHNNSSTPQIQFRDLPGAHPSPTPS----NAGIPTTLTNTSSAPNFANSTFRFPPRVV 332 >SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1150 Score = 25.4 bits (53), Expect = 7.2 Identities = 14/39 (35%), Positives = 21/39 (53%), Gaps = 2/39 (5%) Frame = +3 Query: 228 DDIFVLDVSEDLELENFKAFCEI--ESGFPAKDITLHFN 338 D+ F+ + L +ENF+ FCE+ E D TL +N Sbjct: 1018 DESFLAIEAVHLLIENFEKFCEVPREISLLLDDPTLFYN 1056 >SPBC29A10.10c |||tRNA-splicing endonuclease positive effector |Schizosaccharomyces pombe|chr 2|||Manual Length = 1944 Score = 25.0 bits (52), Expect = 9.5 Identities = 10/26 (38%), Positives = 16/26 (61%) Frame = +3 Query: 483 SLDFSNIQVPTTSANTSMASRNTPVE 560 S SN+ S N ++ASR+TP++ Sbjct: 1788 SKSISNVSTHLASNNLNLASRDTPIK 1813 >SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 2100 Score = 25.0 bits (52), Expect = 9.5 Identities = 15/60 (25%), Positives = 30/60 (50%) Frame = +3 Query: 399 VIILLHMVNSAQNLSVNDSSQALPSGIASLDFSNIQVPTTSANTSMASRNTPVEEDPRII 578 ++ +L + S L N+S Q S + S+D N+ + T N S++ + +D +I+ Sbjct: 575 LVEVLDFLMSRAALQKNESEQKEESVVLSIDTVNVLLTTLMDNVSISEEVSEHIKDVQIL 634 >SPAC823.12 |||zinc finger protein Pep5/Vps11 |Schizosaccharomyces pombe|chr 1|||Manual Length = 906 Score = 25.0 bits (52), Expect = 9.5 Identities = 16/42 (38%), Positives = 20/42 (47%) Frame = -3 Query: 127 KTFYKNCTKTAVISRSY*SI*MTIKQKLWDVPSRSLLTKKKK 2 KT C A I S+ S + KQ+ W S SLL K +K Sbjct: 666 KTLINTCLFEAYIRESFASSNVE-KQEFWQEKSNSLLKKVEK 706 >SPBC4C3.06 |||actin cytoskeletal protein Syp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 818 Score = 25.0 bits (52), Expect = 9.5 Identities = 16/58 (27%), Positives = 28/58 (48%) Frame = +3 Query: 453 SSQALPSGIASLDFSNIQVPTTSANTSMASRNTPVEEDPRIIREMFLANPDQLALLKQ 626 +S ++P G+ L T S N ASR T + P ++ + + ++L +LKQ Sbjct: 136 ASSSVP-GLKGLKKKGRDADTKSQNDLTASRATWDSDAPLAFEKLQIVDEERLLILKQ 192 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,744,689 Number of Sequences: 5004 Number of extensions: 56058 Number of successful extensions: 180 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 179 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 180 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 293780908 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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