Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fprWP01_F_P06
(651 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g13235.1 68416.m01666 ubiquitin family protein contains INTER... 52 3e-07
At1g16190.1 68414.m01939 DNA repair protein RAD23, putative simi... 39 0.003
At2g46500.2 68415.m05791 phosphatidylinositol 3- and 4-kinase fa... 37 0.010
At2g46500.1 68415.m05790 phosphatidylinositol 3- and 4-kinase fa... 37 0.010
At3g06850.2 68416.m00813 branched chain alpha-keto acid dehydrog... 33 0.22
At3g06850.1 68416.m00812 branched chain alpha-keto acid dehydrog... 33 0.22
At5g37640.1 68418.m04533 polyubiquitin (UBQ9) identical to polyu... 32 0.38
At1g53930.1 68414.m06139 ubiquitin family protein contains Pfam ... 31 0.88
At3g09790.1 68416.m01163 polyubiquitin (UBQ8) identical to polyu... 29 2.0
At5g42220.1 68418.m05139 ubiquitin family protein contains INTER... 29 2.7
At3g51220.1 68416.m05607 expressed protein contains Pfam doamin ... 29 3.5
At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearl... 28 4.7
At1g64470.1 68414.m07309 ubiquitin family protein contains INTER... 28 4.7
At1g55860.1 68414.m06406 ubiquitin-protein ligase 1 (UPL1) nearl... 28 4.7
At1g01350.1 68414.m00050 zinc finger (CCCH-type/C3HC4-type RING ... 28 4.7
At4g34960.1 68417.m04955 peptidyl-prolyl cis-trans isomerase, pu... 28 6.2
At2g20470.1 68415.m02390 protein kinase, putative contains prote... 28 6.2
At5g04560.1 68418.m00456 DEMETER protein (DME) identical to DEME... 27 8.2
At1g79680.1 68414.m09293 wall-associated kinase, putative simila... 27 8.2
At1g77460.1 68414.m09020 C2 domain-containing protein / armadill... 27 8.2
>At3g13235.1 68416.m01666 ubiquitin family protein contains
INTERPRO:IPR000626 ubiquitin domain
Length = 414
Score = 52.4 bits (120), Expect = 3e-07
Identities = 31/86 (36%), Positives = 47/86 (54%)
Frame = +3
Query: 201 MKVTVTTLNDDIFVLDVSEDLELENFKAFCEIESGFPAKDITLHFNGKPLLNNKKSLKEH 380
M++TV T + I LDV +EN KA E+ES P + L +NG + N+ K L
Sbjct: 1 MRITVMTAGEQIITLDVDSQETVENVKALLEVESNVPIQQQQLLYNGNEMGNSDK-LSAL 59
Query: 381 GVHDGDVIILLHMVNSAQNLSVNDSS 458
GV D D +L+ MV++A + S ++
Sbjct: 60 GVKDDD--LLMMMVSNASSGSATSAA 83
>At1g16190.1 68414.m01939 DNA repair protein RAD23, putative similar
to DNA repair by nucleotide excision (NER) RAD23
protein, isoform II GI:1914685 from [Daucus carota]
Length = 368
Score = 38.7 bits (86), Expect = 0.003
Identities = 29/127 (22%), Positives = 61/127 (48%), Gaps = 4/127 (3%)
Frame = +3
Query: 201 MKVTVTTLNDDIF---VLDVSEDLELENFKAFCEIESGFPAKDITLHFNGKPLLNNKKSL 371
MK+TV TL F VL + ++ + + +P L NGK L + +
Sbjct: 1 MKLTVKTLKGSHFEIRVLPTDTIMAVKKNIEDSQSKDNYPCGQQLLIHNGKVLKDETTLV 60
Query: 372 KEHGVHDGDVIILLHMVNSAQNLSVNDSSQALPSGIASLDFSNIQVPTTSANTSM-ASRN 548
+ +G ++++L +A + + S+Q + +++ + + P+T+ + ++ AS +
Sbjct: 61 ENKVTEEGFLVVMLSKSKTASSAGPS-STQPTSTTTSTISSTTLAAPSTTQSIAVPASNS 119
Query: 549 TPVEEDP 569
TPV+E P
Sbjct: 120 TPVQEQP 126
>At2g46500.2 68415.m05791 phosphatidylinositol 3- and 4-kinase
family protein / ubiquitin family protein contains
INTERPRO:IPR000626 ubiquitin domain
Length = 566
Score = 37.1 bits (82), Expect = 0.010
Identities = 19/76 (25%), Positives = 40/76 (52%)
Frame = +3
Query: 219 TLNDDIFVLDVSEDLELENFKAFCEIESGFPAKDITLHFNGKPLLNNKKSLKEHGVHDGD 398
TL + + V E +E+ K + GF ++ L F G+ L + +++++GV +G+
Sbjct: 40 TLPGSVIPMRVLESDSIESVKLRIQSYRGFVVRNQKLVFGGRELARSNSNMRDYGVSEGN 99
Query: 399 VIILLHMVNSAQNLSV 446
++ L+ ++ Q L V
Sbjct: 100 ILHLVLKLSDLQVLDV 115
>At2g46500.1 68415.m05790 phosphatidylinositol 3- and 4-kinase
family protein / ubiquitin family protein contains
INTERPRO:IPR000626 ubiquitin domain
Length = 566
Score = 37.1 bits (82), Expect = 0.010
Identities = 19/76 (25%), Positives = 40/76 (52%)
Frame = +3
Query: 219 TLNDDIFVLDVSEDLELENFKAFCEIESGFPAKDITLHFNGKPLLNNKKSLKEHGVHDGD 398
TL + + V E +E+ K + GF ++ L F G+ L + +++++GV +G+
Sbjct: 40 TLPGSVIPMRVLESDSIESVKLRIQSYRGFVVRNQKLVFGGRELARSNSNMRDYGVSEGN 99
Query: 399 VIILLHMVNSAQNLSV 446
++ L+ ++ Q L V
Sbjct: 100 ILHLVLKLSDLQVLDV 115
>At3g06850.2 68416.m00813 branched chain alpha-keto acid
dehydrogenase E2 subunit (din3) identical to branched
chain alpha-keto acid dehydrogenase E2 subunit (din3)
[Arabidopsis thaliana] GI:7021284
Length = 483
Score = 32.7 bits (71), Expect = 0.22
Identities = 21/76 (27%), Positives = 36/76 (47%)
Frame = +3
Query: 249 VSEDLELENFKAFCEIESGFPAKDITLHFNGKPLLNNKKSLKEHGVHDGDVIILLHMVNS 428
V E +E F+ CE++S +IT F GK L + + G+ ++ L + +S
Sbjct: 97 VKEGDSVEEFQPLCEVQSDKATIEITSRFKGKVALISHS--PGDIIKVGETLVRLAVEDS 154
Query: 429 AQNLSVNDSSQALPSG 476
+L DSS+ + G
Sbjct: 155 QDSLLTTDSSEIVTLG 170
>At3g06850.1 68416.m00812 branched chain alpha-keto acid
dehydrogenase E2 subunit (din3) identical to branched
chain alpha-keto acid dehydrogenase E2 subunit (din3)
[Arabidopsis thaliana] GI:7021284
Length = 483
Score = 32.7 bits (71), Expect = 0.22
Identities = 21/76 (27%), Positives = 36/76 (47%)
Frame = +3
Query: 249 VSEDLELENFKAFCEIESGFPAKDITLHFNGKPLLNNKKSLKEHGVHDGDVIILLHMVNS 428
V E +E F+ CE++S +IT F GK L + + G+ ++ L + +S
Sbjct: 97 VKEGDSVEEFQPLCEVQSDKATIEITSRFKGKVALISHS--PGDIIKVGETLVRLAVEDS 154
Query: 429 AQNLSVNDSSQALPSG 476
+L DSS+ + G
Sbjct: 155 QDSLLTTDSSEIVTLG 170
>At5g37640.1 68418.m04533 polyubiquitin (UBQ9) identical to
polyubiquitin (ubq9) gene sequence GI:304120 from
[Arabidopsis thaliana]
Length = 322
Score = 31.9 bits (69), Expect = 0.38
Identities = 18/71 (25%), Positives = 35/71 (49%)
Frame = +3
Query: 198 TMKVTVTTLNDDIFVLDVSEDLELENFKAFCEIESGFPAKDITLHFNGKPLLNNKKSLKE 377
+M++ TL + +DV + N KA + G P L F+GK LL++ ++L +
Sbjct: 2 SMQIHAKTLTEKTITIDVVSSDTINNVKAKIQDIEGIPLDQQRLIFSGK-LLDDGRTLAD 60
Query: 378 HGVHDGDVIIL 410
+ + ++ L
Sbjct: 61 YSIQKDSILHL 71
>At1g53930.1 68414.m06139 ubiquitin family protein contains Pfam
profile: PF00240 ubiquitin family
Length = 158
Score = 30.7 bits (66), Expect = 0.88
Identities = 19/71 (26%), Positives = 34/71 (47%)
Frame = +3
Query: 201 MKVTVTTLNDDIFVLDVSEDLELENFKAFCEIESGFPAKDITLHFNGKPLLNNKKSLKEH 380
M + + TL VL+V +EN KA + + G P L F GK L + +L ++
Sbjct: 86 MFIFIKTLTGRRIVLEVENCDTIENIKAKIQDKQGIPVDQQRLIFKGKQ-LEDGLTLADY 144
Query: 381 GVHDGDVIILL 413
+ + ++ L+
Sbjct: 145 NIQNDSILHLI 155
>At3g09790.1 68416.m01163 polyubiquitin (UBQ8) identical to
polyubiquitin (ubq8) GI:870793, GB:L05917 [Arabidopsis
thaliana] (Genetics 139 (2), 921-939 (1995))
Length = 631
Score = 29.5 bits (63), Expect = 2.0
Identities = 16/51 (31%), Positives = 23/51 (45%)
Frame = +3
Query: 198 TMKVTVTTLNDDIFVLDVSEDLELENFKAFCEIESGFPAKDITLHFNGKPL 350
T+++ TL + LDV + N KA + + G P L F GK L
Sbjct: 2 TIQIYAKTLTEKTITLDVETSDSIHNVKAKIQNKEGIPLDQQRLIFAGKQL 52
Score = 29.1 bits (62), Expect = 2.7
Identities = 17/73 (23%), Positives = 32/73 (43%)
Frame = +3
Query: 195 FTMKVTVTTLNDDIFVLDVSEDLELENFKAFCEIESGFPAKDITLHFNGKPLLNNKKSLK 374
F + + + F+L+V ++N KA + + P L F GKP L ++L
Sbjct: 241 FVKNLPYNSFTGENFILEVESSDTIDNVKAKLQDKERIPMDLHRLIFAGKP-LEGGRTLA 299
Query: 375 EHGVHDGDVIILL 413
+ + G + L+
Sbjct: 300 HYNIQKGSTLYLV 312
Score = 28.7 bits (61), Expect = 3.5
Identities = 22/90 (24%), Positives = 39/90 (43%), Gaps = 2/90 (2%)
Frame = +3
Query: 147 KQ*NLYTVLFI-GFIQKFTMKVTVTTLNDDIFVLDVSEDLELENFKAFCEIESGFPAKDI 323
K+ L+ VL + G +Q F + D L V +EN KA + G
Sbjct: 141 KESTLHLVLRLCGGMQIFVSTFSGKNFTSDTLTLKVESSDTIENVKAKIQDREGLRPDHQ 200
Query: 324 TLHFNGKPLL-NNKKSLKEHGVHDGDVIIL 410
L F+G+ L + ++L ++G+ + + L
Sbjct: 201 RLIFHGEELFTEDNRTLADYGIRNRSTLCL 230
>At5g42220.1 68418.m05139 ubiquitin family protein contains
INTERPRO:IPR000626 ubiquitin domain
Length = 879
Score = 29.1 bits (62), Expect = 2.7
Identities = 17/72 (23%), Positives = 36/72 (50%)
Frame = +3
Query: 198 TMKVTVTTLNDDIFVLDVSEDLELENFKAFCEIESGFPAKDITLHFNGKPLLNNKKSLKE 377
T+++ + TL+ + V+++ + FK E+G P L F G+ +L + L E
Sbjct: 23 TLELNIKTLDSRTYTFQVNKNETVLLFKEKIASETGVPVGQQRLIFRGR-VLKDDHPLSE 81
Query: 378 HGVHDGDVIILL 413
+ + +G + L+
Sbjct: 82 YHLENGHTLHLI 93
>At3g51220.1 68416.m05607 expressed protein contains Pfam doamin
PF05701: Plant protein of unknown function (DUF827)
Length = 186
Score = 28.7 bits (61), Expect = 3.5
Identities = 29/120 (24%), Positives = 52/120 (43%), Gaps = 7/120 (5%)
Frame = +3
Query: 291 EIESGFPAKDI--TLHFNGKPLLNNKKSLKEHGVHDGDVIILLHMVNSAQNLS-VNDSSQ 461
EIE+G P + + + G+ +L + + I +V + +NL + ++
Sbjct: 5 EIETGAPFRSVKEAVTLFGERILLGDNYISKSVERSSCKSIQDELVEAKENLKKAEEENK 64
Query: 462 ALPSGIASLDFSNIQVPTTSANTSMASRNTP----VEEDPRIIREMFLANPDQLALLKQN 629
L I SL ++ TT + + RN P VE+D + I E + PD + +K N
Sbjct: 65 VLSQLIESL---TQELETTKEKLNHSLRNFPEHPQVEDDLKFIEESTVNEPDNITEIKMN 121
>At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearly
identical to ubiquitin-protein ligase 2 [Arabidopsis
thaliana] GI:7108523; E3, HECT-domain protein family;
similar to ubiquitin-protein ligase 2 GI:7108523 from
[Arabidopsis thaliana]
Length = 3658
Score = 28.3 bits (60), Expect = 4.7
Identities = 22/66 (33%), Positives = 33/66 (50%), Gaps = 7/66 (10%)
Frame = -3
Query: 604 SGLARNISLIM--RGSSSTGVFLEAIDVFADVVGTWMLLKS-----RLAMPLGKAWLESL 446
+G+ R + + + R SS+ L+ ++ D + LKS RLA PLGK L+ L
Sbjct: 2740 TGMERGVGVTIGQRDVSSSADGLKVKEIEGDPLVNADALKSLIRLLRLAQPLGKGLLQRL 2799
Query: 445 TLRFCA 428
L CA
Sbjct: 2800 LLNLCA 2805
>At1g64470.1 68414.m07309 ubiquitin family protein contains
INTERPRO:IPR000626 ubiquitin domain
Length = 213
Score = 28.3 bits (60), Expect = 4.7
Identities = 13/38 (34%), Positives = 22/38 (57%)
Frame = +3
Query: 300 SGFPAKDITLHFNGKPLLNNKKSLKEHGVHDGDVIILL 413
+GF + L F+G+ L N +K++GV G V+ L+
Sbjct: 67 NGFRVRRQKLVFSGRELARNASRVKDYGVTGGSVLHLV 104
>At1g55860.1 68414.m06406 ubiquitin-protein ligase 1 (UPL1) nearly
identical to ubiquitin-protein ligase 1 [Arabidopsis
thaliana] GI:7108521; E3, HECT-domain protein family;
similar to GI:7108521, GB:AAF36454 from [Arabidopsis
thaliana]
Length = 3891
Score = 28.3 bits (60), Expect = 4.7
Identities = 22/66 (33%), Positives = 33/66 (50%), Gaps = 7/66 (10%)
Frame = -3
Query: 604 SGLARNISLIM--RGSSSTGVFLEAIDVFADVVGTWMLLKS-----RLAMPLGKAWLESL 446
+G+ R + + + R SS+ L+ ++ D + LKS RLA PLGK L+ L
Sbjct: 2967 TGMDRGVGVTIGQRAVSSSADGLKVKEIEGDPLVNADALKSLIRLLRLAQPLGKGLLQRL 3026
Query: 445 TLRFCA 428
L CA
Sbjct: 3027 LLNLCA 3032
>At1g01350.1 68414.m00050 zinc finger (CCCH-type/C3HC4-type RING
finger) family protein similar to SP|O15541 Zinc finger
protein 183 {Homo sapiens}; contains Pfam profiles
PF04396: Protein of unknown function, DUF537, PF00097:
Zinc finger, C3HC4 type (RING finger), PF00642: Zinc
finger C-x8-C-x5-C-x3-H type (and similar)
Length = 586
Score = 28.3 bits (60), Expect = 4.7
Identities = 23/76 (30%), Positives = 32/76 (42%), Gaps = 1/76 (1%)
Frame = +3
Query: 423 NSAQNLSVNDSSQALPSGIASLDFSN-IQVPTTSANTSMASRNTPVEEDPRIIREMFLAN 599
+S + S +S A + D S IQV S T+ T +D R IRE L
Sbjct: 79 SSGPSKSSTTTSGAPERSVFHYDSSKEIQVQNDSGATATLETETDFNQDARAIRERVLKK 138
Query: 600 PDQLALLKQNNPRLAD 647
D+ LK N + +D
Sbjct: 139 ADE--ALKGNKKKASD 152
>At4g34960.1 68417.m04955 peptidyl-prolyl cis-trans isomerase,
putative / cyclophilin, putative / rotamase, putative
similar to cyclophilin [Arabidopsis thaliana]
gi|2443755|gb|AAB71401
Length = 224
Score = 27.9 bits (59), Expect = 6.2
Identities = 14/46 (30%), Positives = 22/46 (47%)
Frame = +3
Query: 267 LENFKAFCEIESGFPAKDITLHFNGKPLLNNKKSLKEHGVHDGDVI 404
+ENF+A C E G + LH+ G P + + + GD+I
Sbjct: 76 VENFRALCTGEKGKTSSGKPLHYKGTPF---HRIISGFVIQGGDII 118
>At2g20470.1 68415.m02390 protein kinase, putative contains protein
kinase domain, Pfam:PF00069
Length = 596
Score = 27.9 bits (59), Expect = 6.2
Identities = 12/25 (48%), Positives = 18/25 (72%)
Frame = +3
Query: 228 DDIFVLDVSEDLELENFKAFCEIES 302
D FV +V++DL+ +NF+ F E ES
Sbjct: 433 DAAFVPEVNDDLDTQNFEKFDESES 457
>At5g04560.1 68418.m00456 DEMETER protein (DME) identical to DEMETER
protein [Arabidopsis thaliana] GI:21743571; contains Pfam
profile PF00730: HhH-GPD superfamily base excision DNA
repair protein
Length = 1729
Score = 27.5 bits (58), Expect = 8.2
Identities = 14/57 (24%), Positives = 30/57 (52%)
Frame = +1
Query: 424 IPHRISALMIPAKPCQVA*LALTSATSKYLRHQQIHQWLRETRLLKKTHA*LERCFL 594
+P + +IP+ P ++ + L+ ++S HQ Q ++ + K +H L++ FL
Sbjct: 1050 VPREFTGQIIPSTPHELPGMGLSGSSSAVQEHQDDTQHNQQDEMNKASH--LQKTFL 1104
>At1g79680.1 68414.m09293 wall-associated kinase, putative similar
to wall-associated kinase 2 GI:4826399 from [Arabidopsis
thaliana]
Length = 769
Score = 27.5 bits (58), Expect = 8.2
Identities = 11/29 (37%), Positives = 20/29 (68%)
Frame = -2
Query: 95 RYKQILLEYLNDDQTKTLGRSLAFLADEK 9
RY+QI+ ++D T++ G +AF+ DE+
Sbjct: 204 RYQQIIGVEIDDSNTESKGCKVAFITDEE 232
>At1g77460.1 68414.m09020 C2 domain-containing protein /
armadillo/beta-catenin repeat family protein similar to
CCLS 65 [Silene latifolia] GI:2570102; contains Pfam
profiles PF00514: Armadillo/beta-catenin-like repeat,
PF00168: C2 domain
Length = 2110
Score = 27.5 bits (58), Expect = 8.2
Identities = 21/68 (30%), Positives = 33/68 (48%), Gaps = 6/68 (8%)
Frame = -2
Query: 188 DKPNK*HSVEVLLLYIYLNFKNILQKLYKNGRYKQI--LLEYLNDDQTKTLG----RSLA 27
D PN + LL + L+F + +QK+ +G K + LLE ND + +L+
Sbjct: 200 DNPNSQANAASLLARLVLSFCDSIQKILNSGVVKSLIQLLEQKNDINVRASAADALEALS 259
Query: 26 FLADEKKK 3
+DE KK
Sbjct: 260 ANSDEAKK 267
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,963,204
Number of Sequences: 28952
Number of extensions: 279710
Number of successful extensions: 860
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 838
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 860
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1354097952
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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