Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fprWP01_F_P03
(654 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 100 4e-22
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 56 4e-09
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 54 2e-08
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 49 5e-07
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 48 1e-06
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 34 0.021
SPCC1183.11 ||SPCC31H12.01|MS ion channel protein 1|Schizosaccha... 28 1.0
SPAC1327.01c ||SPAC1783.09c, SPAC18G6.16c|transcription factor, ... 27 3.1
SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr 3|||Ma... 25 7.2
SPBC29A10.07 |||nucleoporin Pom152|Schizosaccharomyces pombe|chr... 25 7.2
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 99.5 bits (237), Expect = 4e-22
Identities = 47/59 (79%), Positives = 52/59 (88%)
Frame = +1
Query: 106 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGEYT 282
LT+EQIAEF+EAFSLFD+D DG IT+ ELG VMRSLGQ+PT AELQDMINEVDADG T
Sbjct: 6 LTDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGT 64
Score = 60.1 bits (139), Expect = 3e-10
Identities = 30/71 (42%), Positives = 46/71 (64%)
Frame = +1
Query: 94 MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADG 273
M D EE++ +EAF +FDKDG+G IT +EL V+ SLG+ ++ E+ DMI E D DG
Sbjct: 78 MKDTDNEEEV---REAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDG 134
Query: 274 EYT*NHMIYAK 306
+ N+ +++
Sbjct: 135 DGVINYEEFSR 145
Score = 31.5 bits (68), Expect = 0.11
Identities = 17/51 (33%), Positives = 26/51 (50%), Gaps = 1/51 (1%)
Frame = +1
Query: 124 AEFKEAFSLFDKDGDGTITTKELGTVM-RSLGQNPTEAELQDMINEVDADG 273
AE ++ + D DG+GTI E T+M R + E E+++ D DG
Sbjct: 48 AELQDMINEVDADGNGTIDFTEFLTMMARKMKDTDNEEEVREAFKVFDKDG 98
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 56.0 bits (129), Expect = 4e-09
Identities = 27/51 (52%), Positives = 36/51 (70%)
Frame = +1
Query: 109 TEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEV 261
++EQ E KEAF L+D D DG I T +G+V+RSLG N T+AEL + NE+
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNEL 54
Score = 43.2 bits (97), Expect = 3e-05
Identities = 22/60 (36%), Positives = 37/60 (61%), Gaps = 1/60 (1%)
Frame = +1
Query: 88 STMADQLTE-EQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVD 264
S ++++L E E E+ +AF +FDKD G I T + M++LG+ ++ E+Q M+ E D
Sbjct: 65 SFVSNKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEAD 124
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 54.0 bits (124), Expect = 2e-08
Identities = 24/54 (44%), Positives = 35/54 (64%)
Frame = +1
Query: 103 QLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVD 264
QLT QI E KEAF+L DKDGDG I +++ T++ SL Q+ +E + M ++
Sbjct: 41 QLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHMFESIN 94
Score = 26.2 bits (55), Expect = 4.1
Identities = 12/48 (25%), Positives = 21/48 (43%)
Frame = +1
Query: 136 EAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGEY 279
EAFS FD G I + + S+G E++ ++ + G +
Sbjct: 121 EAFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEVESILRSYTSHGVF 168
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 49.2 bits (112), Expect = 5e-07
Identities = 23/58 (39%), Positives = 34/58 (58%)
Frame = +1
Query: 103 QLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGE 276
++TEEQ + EAF LFD D D I EL MR+LG N ++E+ ++ + D G+
Sbjct: 30 EITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGK 87
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 48.0 bits (109), Expect = 1e-06
Identities = 20/47 (42%), Positives = 33/47 (70%)
Frame = +1
Query: 130 FKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDAD 270
+K+AFSLFD+ G G I +G ++R+ GQNPT AE+ ++ + + A+
Sbjct: 8 YKQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEITEIESTLPAE 54
Score = 37.1 bits (82), Expect = 0.002
Identities = 17/45 (37%), Positives = 26/45 (57%)
Frame = +1
Query: 127 EFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEV 261
EF + F +FDKD G I EL V+ SLG+ + E+ +++ V
Sbjct: 78 EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGV 122
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 33.9 bits (74), Expect = 0.021
Identities = 19/54 (35%), Positives = 29/54 (53%)
Frame = +1
Query: 115 EQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGE 276
E E +EAF LFD G I ++L LG+N T+ +LQ M++ +G+
Sbjct: 9 EMDEEAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLDLAGTNGK 62
>SPCC1183.11 ||SPCC31H12.01|MS ion channel protein
1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1011
Score = 28.3 bits (60), Expect = 1.0
Identities = 15/56 (26%), Positives = 29/56 (51%), Gaps = 2/56 (3%)
Frame = +1
Query: 100 DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLG--QNPTEAELQDMINEV 261
D+ E A + +S+FD+D +G IT +E+ +G + A L+D+ + +
Sbjct: 538 DETGEVDNATLEACYSIFDRDLNGDITCEEIELACVEIGKERKSISASLRDLNDSI 593
>SPAC1327.01c ||SPAC1783.09c, SPAC18G6.16c|transcription factor,
zf-fungal binuclear cluster type |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 977
Score = 26.6 bits (56), Expect = 3.1
Identities = 9/27 (33%), Positives = 15/27 (55%)
Frame = -3
Query: 244 LEVRLLWGSVLATSSRCPALWS*WCHR 164
LE++ WGS+ A + +W+ W R
Sbjct: 850 LEMKNYWGSISALCDKMSEIWADWVQR 876
>SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 630
Score = 25.4 bits (53), Expect = 7.2
Identities = 9/24 (37%), Positives = 14/24 (58%)
Frame = +1
Query: 136 EAFSLFDKDGDGTITTKELGTVMR 207
+ F FD+D DG + +EL + R
Sbjct: 311 DLFYQFDRDNDGALNNEELSALFR 334
>SPBC29A10.07 |||nucleoporin Pom152|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1250
Score = 25.4 bits (53), Expect = 7.2
Identities = 11/41 (26%), Positives = 22/41 (53%)
Frame = +1
Query: 175 ITTKELGTVMRSLGQNPTEAELQDMINEVDADGEYT*NHMI 297
++ + L ++ S +NP E LQ + + + G YT H++
Sbjct: 212 VSGRSLRKLLSSSSKNPKEPRLQTIYLKTNKRGLYTLKHVV 252
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,969,772
Number of Sequences: 5004
Number of extensions: 31941
Number of successful extensions: 74
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 68
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 74
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 295793106
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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