BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fprWP01_F_N14 (508 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_37955| Best HMM Match : 14-3-3 (HMM E-Value=6.5861e-44) 155 2e-38 SB_34217| Best HMM Match : No HMM Matches (HMM E-Value=.) 146 1e-35 SB_36368| Best HMM Match : 14-3-3 (HMM E-Value=0) 134 3e-32 SB_34219| Best HMM Match : No HMM Matches (HMM E-Value=.) 118 4e-27 SB_34218| Best HMM Match : No HMM Matches (HMM E-Value=.) 73 2e-13 SB_56522| Best HMM Match : Pox_A_type_inc (HMM E-Value=1.6e-30) 36 0.015 SB_20371| Best HMM Match : U-box (HMM E-Value=0.075) 33 0.10 SB_22570| Best HMM Match : Filament (HMM E-Value=0.1) 31 0.41 SB_12729| Best HMM Match : Pox_A_type_inc (HMM E-Value=5.3e-08) 31 0.72 SB_7846| Best HMM Match : CoCoA (HMM E-Value=0.00016) 31 0.72 SB_43756| Best HMM Match : SlyX (HMM E-Value=3.7) 30 1.3 SB_36640| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 1.7 SB_37483| Best HMM Match : Drf_FH1 (HMM E-Value=6.6) 29 2.9 SB_2776| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 3.8 SB_29939| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 3.8 SB_47663| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 5.1 SB_44921| Best HMM Match : Pepsin-I3 (HMM E-Value=6.4) 28 5.1 SB_39167| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 5.1 SB_34997| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 5.1 SB_10819| Best HMM Match : DCX (HMM E-Value=6.3e-19) 28 5.1 SB_15785| Best HMM Match : No HMM Matches (HMM E-Value=.) 27 6.7 SB_42553| Best HMM Match : VLPT (HMM E-Value=1) 27 8.9 SB_40561| Best HMM Match : Keratin_B2 (HMM E-Value=1.1) 27 8.9 SB_31531| Best HMM Match : No HMM Matches (HMM E-Value=.) 27 8.9 SB_11774| Best HMM Match : M (HMM E-Value=8.5e-09) 27 8.9 >SB_37955| Best HMM Match : 14-3-3 (HMM E-Value=6.5861e-44) Length = 251 Score = 155 bits (376), Expect = 2e-38 Identities = 80/128 (62%), Positives = 100/128 (78%), Gaps = 1/128 (0%) Frame = +2 Query: 77 MSEREDNVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNLLSVAYKNVIGARR 256 M+++E++VY AKLAEQAERYD+MV +MK VA EL+ E+RNLLSVAYKNVIGARR Sbjct: 1 MADKEEHVYMAKLAEQAERYDDMVNSMKEVAKMGT---ELSTEDRNLLSVAYKNVIGARR 57 Query: 257 ASWRIISSIEQKEETKGAE-DKLNMIRAYRSQVEKELRDICSDILGVLDKYLIPSSQTGE 433 ASWRII+SIEQKEE+KG + KL MIR YR +E+EL+ IC +IL +LD LI +SQ+ E Sbjct: 58 ASWRIITSIEQKEESKGEDMAKLEMIRNYRKTIEEELKTICGEILSLLDDSLIKNSQSEE 117 Query: 434 SXVXYYKM 457 S V Y K+ Sbjct: 118 SKVFYNKI 125 >SB_34217| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 219 Score = 146 bits (353), Expect = 1e-35 Identities = 69/143 (48%), Positives = 104/143 (72%) Frame = +2 Query: 77 MSEREDNVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNLLSVAYKNVIGARR 256 M RE +Y AKL+EQ +RYDEM + MK V+ + +L+ EERNLLSV+YKN++G RR Sbjct: 81 MVVRETLIYNAKLSEQCDRYDEMAKIMKEVSEKYP---KLSKEERNLLSVSYKNIVGQRR 137 Query: 257 ASWRIISSIEQKEETKGAEDKLNMIRAYRSQVEKELRDICSDILGVLDKYLIPSSQTGES 436 +SWR+ISSIE+K L +++ Y++ +EKEL+D+C ++LG+L++ LIP ++ E+ Sbjct: 138 SSWRVISSIEEK---TAESSSLAIVKKYKACIEKELKDLCKEVLGILER-LIPGAEDEEN 193 Query: 437 XVXYYKMKGDYHRYLAXFATGND 505 V Y+K+KGDY+RYLA F+ G + Sbjct: 194 KVFYFKLKGDYYRYLAEFSHGQE 216 >SB_36368| Best HMM Match : 14-3-3 (HMM E-Value=0) Length = 248 Score = 134 bits (325), Expect = 3e-32 Identities = 70/144 (48%), Positives = 93/144 (64%), Gaps = 3/144 (2%) Frame = +2 Query: 86 REDNVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNLLSVAYKNVIGARRASW 265 RE+ ++ AK+AEQAERYD+MV AM V N+ EERNLLSVAYKNV+GARR+SW Sbjct: 11 REELIHLAKMAEQAERYDDMVNAMSAVTKEGKPLND---EERNLLSVAYKNVVGARRSSW 67 Query: 266 RIISSIEQKEETKGAEDKLNMIRAYRSQVEKELRDICSDILGVLDKYLIPSSQ---TGES 436 R+ISS+EQ K E+ + + YR + EL C+++L +L+ YL+ Q E+ Sbjct: 68 RVISSMEQ----KAPEEMAALTKKYREDITNELNGKCAEVLDILENYLLKDGQDDINTEA 123 Query: 437 XVXYYKMKGDYHRYLAXFATGNDR 508 V Y KM+GDYHRYL A G+ R Sbjct: 124 KVFYLKMRGDYHRYLVEVAEGDSR 147 >SB_34219| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 309 Score = 118 bits (283), Expect = 4e-27 Identities = 64/122 (52%), Positives = 82/122 (67%) Frame = +2 Query: 143 MVEAMKNVASRNVSDNELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEETKGAEDKL 322 M +AMK +S+ L EERNLLSVAYKNV+GA+R+SWR+ISSIEQK E G+E K Sbjct: 1 MAKAMKEATE--ISET-LEQEERNLLSVAYKNVVGAKRSSWRVISSIEQKLE--GSERKK 55 Query: 323 NMIRAYRSQVEKELRDICSDILGVLDKYLIPSSQTGESXVXYYKMKGDYHRYLAXFATGN 502 YR +E EL ++C +L +L+ LIP++Q+ ES V Y KMKGDY+RY A G Sbjct: 56 QNTETYRQTIENELNEVCETVLKLLESKLIPNAQSTESKVFYLKMKGDYYRYEGEVA-GA 114 Query: 503 DR 508 DR Sbjct: 115 DR 116 >SB_34218| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 607 Score = 72.5 bits (170), Expect = 2e-13 Identities = 42/113 (37%), Positives = 64/113 (56%), Gaps = 3/113 (2%) Frame = +2 Query: 59 NHLSSTMS---EREDNVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNLLSVA 229 NH S + R + V AKLAEQ ER+++++ MK N S N+ E RNLLSV Sbjct: 178 NHPSGVVQMQDSRNELVQLAKLAEQTERFEDVILYMKKAIEINPSLNK---EHRNLLSVG 234 Query: 230 YKNVIGARRASWRIISSIEQKEETKGAEDKLNMIRAYRSQVEKELRDICSDIL 388 YKNV+G++R +WR + + + +L I Y+ ++E EL+ +C +IL Sbjct: 235 YKNVVGSKRFAWRHLHHDALQSGRYIRDSQLKGIIKYKEKIEMELKTLCREIL 287 >SB_56522| Best HMM Match : Pox_A_type_inc (HMM E-Value=1.6e-30) Length = 3071 Score = 36.3 bits (80), Expect = 0.015 Identities = 29/114 (25%), Positives = 58/114 (50%), Gaps = 9/114 (7%) Frame = +2 Query: 89 EDNVYKAKLAEQAERYDEMV----EAMKNVASRNVSDNELTVEE----RNLLSVAYKNVI 244 + N+ K KL + E Y + + +A +N N N+L +E R+L S+ + N + Sbjct: 1955 DPNLQK-KLKDLEESYQKALKDNEDAERNYLDLNRKYNKLVMEHGNISRDLESLKHDNDL 2013 Query: 245 GARRA-SWRIISSIEQKEETKGAEDKLNMIRAYRSQVEKELRDICSDILGVLDK 403 R ++ Q++E + A++K+N + AY++ E++LR+ D++ K Sbjct: 2014 KNERIIDLESSLAVYQEQELQSAKNKINRLEAYKTSYEEKLRNQQEDLVAAQQK 2067 >SB_20371| Best HMM Match : U-box (HMM E-Value=0.075) Length = 515 Score = 33.5 bits (73), Expect = 0.10 Identities = 22/88 (25%), Positives = 42/88 (47%), Gaps = 3/88 (3%) Frame = +2 Query: 62 HLSSTMSEREDNVYKAKLAEQAERYDEM---VEAMKNVASRNVSDNELTVEERNLLSVAY 232 H+ M + D V +AK A+ YD + ++ + + + D E +++LL+ + Sbjct: 345 HMEREMDQWMDAVQQAK-AQFTREYDPAKSEIPDIETIFQKKIEDLESANNDKDLLN--H 401 Query: 233 KNVIGARRASWRIISSIEQKEETKGAED 316 K ++G + W++ EQ GAED Sbjct: 402 KKIVGFDKKIWKVHHEKEQMIGAGGAED 429 >SB_22570| Best HMM Match : Filament (HMM E-Value=0.1) Length = 601 Score = 31.5 bits (68), Expect = 0.41 Identities = 29/105 (27%), Positives = 52/105 (49%), Gaps = 4/105 (3%) Frame = +2 Query: 59 NHLSSTMSEREDNVYKAKLAEQAERYDEMVEAM----KNVASRNVSDNELTVEERNLLSV 226 N L ST++ER + ++ L E R+DE + ++ KN+ R S N+L +N S Sbjct: 217 NSLVSTLNERLERK-ESTLMECRRRHDEELTSLQKKIKNLEERLSSTNQLISSTKNDESG 275 Query: 227 AYKNVIGARRASWRIISSIEQKEETKGAEDKLNMIRAYRSQVEKE 361 + +I ++ I+ +E+ + AE +L M A ++E E Sbjct: 276 RIQALIKRVCLQYKRINRLEETRHQEKAEMELKM--AQLKEIENE 318 >SB_12729| Best HMM Match : Pox_A_type_inc (HMM E-Value=5.3e-08) Length = 1183 Score = 30.7 bits (66), Expect = 0.72 Identities = 26/109 (23%), Positives = 50/109 (45%), Gaps = 1/109 (0%) Frame = +2 Query: 59 NHLSSTMSERED-NVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNLLSVAYK 235 N ++ ER D +V L Q R + + MK R+ ++ + +E++ L A Sbjct: 430 NSVNVLRKERVDLDVQVTNLRSQKIRLEAEISVMKEERRRDDNEVDHLRKEKSDLEKAMH 489 Query: 236 NVIGARRASWRIISSIEQKEETKGAEDKLNMIRAYRSQVEKELRDICSD 382 ++ R ++ + I E+T + + +R +EKELRD+ S+ Sbjct: 490 SL---RSEKTKVETDITMAEDTWRKRNDTSELRFEADDLEKELRDLRSE 535 >SB_7846| Best HMM Match : CoCoA (HMM E-Value=0.00016) Length = 1284 Score = 30.7 bits (66), Expect = 0.72 Identities = 22/92 (23%), Positives = 48/92 (52%), Gaps = 2/92 (2%) Frame = +2 Query: 101 YKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNLLSVAYKNVIGARRASWRIISS 280 YK K E + ++ +A ++ S N SDNE+ + RN +S + + R +S + Sbjct: 1024 YKIKFIEWRKLQSQLQKARRSSPSVN-SDNEVCHDARNTISKLREEMEELRESSAAAQKA 1082 Query: 281 IEQKEETKGAEDKLN--MIRAYRSQVEKELRD 370 +EQ+++ + + ++N I R +++K + + Sbjct: 1083 LEQEKQQRLEKLEMNNQEIMFLREELQKAVEE 1114 >SB_43756| Best HMM Match : SlyX (HMM E-Value=3.7) Length = 179 Score = 29.9 bits (64), Expect = 1.3 Identities = 16/80 (20%), Positives = 39/80 (48%), Gaps = 1/80 (1%) Frame = +2 Query: 65 LSSTMSEREDNVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEER-NLLSVAYKNV 241 LSS +SER + + +L + E Y ++ ++ R+ +L ++ L+++++ Sbjct: 53 LSSVLSERCGELERRQLEMEREFYTKIQALEAQISDRSEEWKDLLAQDHAKLMTISHDTT 112 Query: 242 IGARRASWRIISSIEQKEET 301 + W++ + KE+T Sbjct: 113 YDLKSGYWQVEMDPKDKEKT 132 >SB_36640| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 283 Score = 29.5 bits (63), Expect = 1.7 Identities = 18/47 (38%), Positives = 20/47 (42%) Frame = -3 Query: 221 TAGSSPPLSAHCRTRCVMLHSSSPPPFRHSAQPAQLTLLYTHYLPFP 81 TA S PP +A C T C + P P P T L H LP P Sbjct: 40 TALSPPPATALCPTPC---YGPVPHPLLRPCVPPPATALVPHPLPRP 83 >SB_37483| Best HMM Match : Drf_FH1 (HMM E-Value=6.6) Length = 237 Score = 28.7 bits (61), Expect = 2.9 Identities = 17/48 (35%), Positives = 21/48 (43%), Gaps = 1/48 (2%) Frame = -3 Query: 161 SSSPPPFRH-SAQPAQLTLLYTHYLPFPTLWMTNDFTLTKSESPGHLF 21 +S P P+ S QPA T YT Y P T+ T + SP F Sbjct: 73 ASQPAPYTPASGQPASYTPAYTPYTPASTVGRMPPTTSQSTPSPAPFF 120 >SB_2776| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1792 Score = 28.3 bits (60), Expect = 3.8 Identities = 17/53 (32%), Positives = 29/53 (54%) Frame = +2 Query: 74 TMSEREDNVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNLLSVAY 232 T+SERE KA++ A E A ++A + ++NE +E R+L +A+ Sbjct: 788 TISEREAIANKARIRSGALTLSETGPAFVDLALKVPAENEEKLENRDLEEMAW 840 >SB_29939| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1122 Score = 28.3 bits (60), Expect = 3.8 Identities = 13/43 (30%), Positives = 22/43 (51%) Frame = +2 Query: 92 DNVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNLL 220 D VY+ L RY+E+V + N + +N TV +R ++ Sbjct: 80 DEVYRPYLTTDETRYNEVVFVVDNTVYQRYHNNTKTVTDRVII 122 >SB_47663| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1409 Score = 27.9 bits (59), Expect = 5.1 Identities = 13/40 (32%), Positives = 19/40 (47%) Frame = +2 Query: 92 DNVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEER 211 D VY+ L RY EMV + N + +N TV ++ Sbjct: 170 DEVYRPYLTTDETRYTEMVFVVDNTVYQRYQNNTKTVTDK 209 >SB_44921| Best HMM Match : Pepsin-I3 (HMM E-Value=6.4) Length = 105 Score = 27.9 bits (59), Expect = 5.1 Identities = 18/84 (21%), Positives = 36/84 (42%) Frame = +2 Query: 59 NHLSSTMSEREDNVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNLLSVAYKN 238 N + ER + ++ + +D ++ NV R + L + L+ A Sbjct: 21 NKTAELYKERAKALSRSPEETRYSSFDTSLDYNGNVLHRGDLEASLNHQLTEKLAEAESE 80 Query: 239 VIGARRASWRIISSIEQKEETKGA 310 ++ +R + +IS +EQK +GA Sbjct: 81 ILASRMSRDNLISQVEQKIAARGA 104 >SB_39167| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 960 Score = 27.9 bits (59), Expect = 5.1 Identities = 18/84 (21%), Positives = 36/84 (42%) Frame = +2 Query: 59 NHLSSTMSEREDNVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNLLSVAYKN 238 N + ER + ++ + +D ++ NV R + L + L+ A Sbjct: 98 NKTAELYKERAKALSRSPEETRYSSFDTSLDYNGNVLHRGDLEASLNHQLTEKLAEAESE 157 Query: 239 VIGARRASWRIISSIEQKEETKGA 310 ++ +R + +IS +EQK +GA Sbjct: 158 ILASRMSRDNLISQVEQKIAARGA 181 >SB_34997| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 845 Score = 27.9 bits (59), Expect = 5.1 Identities = 16/47 (34%), Positives = 25/47 (53%) Frame = -2 Query: 414 GMRYLSRTPRMSEQMSRSSFSTWLRYALIMFNLSSAPFVSSFCSMEE 274 G R RTP ++ + S +W YALI+F+L F+ C++ E Sbjct: 77 GSRLKGRTPHLTRALM--SILSWSEYALILFSL----FIGVACNILE 117 >SB_10819| Best HMM Match : DCX (HMM E-Value=6.3e-19) Length = 1199 Score = 27.9 bits (59), Expect = 5.1 Identities = 17/49 (34%), Positives = 21/49 (42%), Gaps = 3/49 (6%) Frame = +1 Query: 193 ADSGGEEPAVGRLQERDRRPPRLLADHLLHR---TEGRNKRRRGQIEHD 330 A GG +GR E DR P+ R EG +RRRG+ D Sbjct: 1082 AQKGGYASQIGRRNEPDREQPKQRVGRRDERDPDDEGTGRRRRGRERDD 1130 >SB_15785| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 600 Score = 27.5 bits (58), Expect = 6.7 Identities = 24/103 (23%), Positives = 47/103 (45%), Gaps = 1/103 (0%) Frame = +2 Query: 104 KAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNLLSVAYKNVIGARRASWRIISSI 283 K + A E ++AMK++ ++ + +ER + A++N I R S++ Sbjct: 248 KTESMAMASSSQEQIKAMKDLLAKARDEITSLKDEREKMITAHQNRIEQLRESFK----- 302 Query: 284 EQKEETKGAEDKLN-MIRAYRSQVEKELRDICSDILGVLDKYL 409 ++ EE DKLN I + + +KE ++ ++ K L Sbjct: 303 KKMEEADAWPDKLNKAILEEQEKFKKEKDELWKELNDSFQKEL 345 >SB_42553| Best HMM Match : VLPT (HMM E-Value=1) Length = 399 Score = 27.1 bits (57), Expect = 8.9 Identities = 14/44 (31%), Positives = 25/44 (56%) Frame = +2 Query: 71 STMSEREDNVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTV 202 +T E+ + + K A Q+E DE++E M+ +RNV + T+ Sbjct: 54 TTDCEQLELYQQVKKAVQSENIDEVLEIMRCAKARNVVPHTKTI 97 >SB_40561| Best HMM Match : Keratin_B2 (HMM E-Value=1.1) Length = 1139 Score = 27.1 bits (57), Expect = 8.9 Identities = 11/33 (33%), Positives = 18/33 (54%) Frame = -3 Query: 191 HCRTRCVMLHSSSPPPFRHSAQPAQLTLLYTHY 93 +C T V+L S P +R +PA +T T++ Sbjct: 518 NCHTNSVILSSRKRPTYRSRKRPAPITTTITNH 550 >SB_31531| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 287 Score = 27.1 bits (57), Expect = 8.9 Identities = 31/105 (29%), Positives = 44/105 (41%), Gaps = 3/105 (2%) Frame = +2 Query: 83 EREDNVYKAKLAEQAERYDEMVEAMKNVASRNVSDN-ELTVEERNLLSVAYKNVIGARRA 259 ERE KAKL + +RY E + + N DN E V+E V K V G + Sbjct: 129 ERERTTNKAKLLLKKKRYQEQLLQRTD----NQLDNLEKLVQEIEFAQVQIKVVEGLKTG 184 Query: 260 SWRIISSIEQKEETKGAEDKLNMIRAYRSQVE--KELRDICSDIL 388 + + Q E ED ++ + VE KE+ D+ S L Sbjct: 185 N----ECLNQLHEMMSIEDVERIMDETKEAVEYQKEIDDLLSGSL 225 >SB_11774| Best HMM Match : M (HMM E-Value=8.5e-09) Length = 1998 Score = 27.1 bits (57), Expect = 8.9 Identities = 26/110 (23%), Positives = 53/110 (48%), Gaps = 3/110 (2%) Frame = +2 Query: 83 EREDNVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNL--LSVAYKNVIGARR 256 E+ N ++ L + ++Y+E++EA++ S+ VEER L L+ A +G Sbjct: 1651 EKMKNDHEQDLTSERKKYEEIIEALQGELSK-AEVRLKEVEERLLSALAEAKSQKLGNDP 1709 Query: 257 ASWRIISSIEQKEETKGAEDKLNM-IRAYRSQVEKELRDICSDILGVLDK 403 S I + K KG E + + + A + + E+ L+ + ++ + +K Sbjct: 1710 ESPTSIDESDIKLIKKGLEFEYDQKLSAEKRKFEETLQGLRKEVGNLQEK 1759 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,580,998 Number of Sequences: 59808 Number of extensions: 293646 Number of successful extensions: 890 Number of sequences better than 10.0: 25 Number of HSP's better than 10.0 without gapping: 816 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 879 length of database: 16,821,457 effective HSP length: 77 effective length of database: 12,216,241 effective search space used: 1111677931 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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