BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fprWP01_F_M14 (613 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 260 1e-70 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 150 1e-37 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 149 4e-37 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 121 9e-29 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 78 9e-16 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 76 4e-15 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 72 6e-14 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 49 5e-07 SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 31 0.13 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 27 1.6 SPCC757.09c |rnc1||RNA-binding protein that suppresses calcineur... 27 2.1 SPBC21D10.12 |hob1||BAR adaptor protein Hob1|Schizosaccharomyces... 27 2.1 SPAC17C9.01c |nuc2|apc3, SPAC1851.01|anaphase-promoting complex ... 25 6.5 SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 25 8.6 SPBC4C3.12 |sep1||fork head transcription factor Sep1|Schizosacc... 25 8.6 SPBC1683.09c |frp1||ferric-chelate reductase Frp1|Schizosaccharo... 25 8.6 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 260 bits (636), Expect = 1e-70 Identities = 124/165 (75%), Positives = 133/165 (80%) Frame = +2 Query: 116 KKLPRLVVDNGSGYVQGXXXXXXXXXXXVPLXSWEGPAIRXVMVGMGQKDSYVGDEAQSK 295 +++ LV+DNGSG + P P +MVGMGQKDSYVGDEAQSK Sbjct: 3 EEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGR-PRHHGIMVGMGQKDSYVGDEAQSK 61 Query: 296 RGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTX 475 RGILTLKYPIEHGIV NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NREKMT Sbjct: 62 RGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQ 121 Query: 476 IMFETFNTPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIY 610 I+FETFN PA YVAIQAVLSLYASGRTTGIVLDSGDGV+HTVPIY Sbjct: 122 IIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGDGVTHTVPIY 166 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 150 bits (364), Expect = 1e-37 Identities = 74/161 (45%), Positives = 102/161 (63%), Gaps = 1/161 (0%) Frame = +2 Query: 131 LVVDNGSGYVQGXXXXXXXXXXXVPLXSWEGPAIRXVMVGMGQKDSYVGDEAQSKRGILT 310 + +DNGSG+++ P R VM QKD +VG EAQ+ RG+L Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHER-VMPSSIQKDMFVGSEAQNLRGLLK 70 Query: 311 LKYPIEHGIVTNWDDMEKIWHHTFYNE-LRVAPEEHPVLLTEAPLNPKANREKMTXIMFE 487 ++ PIE GI+ NW DME+IW + + ++ L PEEHP+LLTE PL N+EK+ +E Sbjct: 71 IQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEYFYE 130 Query: 488 TFNTPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIY 610 T N PA+ ++Q VL+LYAS RTTGIVL+ GDG++H+VPIY Sbjct: 131 TLNVPALSFSLQPVLALYASARTTGIVLECGDGLTHSVPIY 171 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 149 bits (360), Expect = 4e-37 Identities = 73/162 (45%), Positives = 107/162 (66%), Gaps = 2/162 (1%) Frame = +2 Query: 131 LVVDNGSGYVQGXXXXXXXXXXXVPLXSWEGPAIRXV-MVGMGQ-KDSYVGDEAQSKRGI 304 +V+DNG+G+V+ P P +R G Q KD VGDEA++ R + Sbjct: 6 IVLDNGTGFVKVGYAKDNFPRFQFPSIVGR-PILRAEEKTGNVQIKDVMVGDEAEAVRSL 64 Query: 305 LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTXIMF 484 L +KYP+E+GI+ ++++M ++W +TF+ +L++ P +LLTE P+NP ANREKM MF Sbjct: 65 LQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCETMF 124 Query: 485 ETFNTPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIY 610 E + +YVAIQAVLSLYA G ++G+V+DSGDGV+H VP+Y Sbjct: 125 ERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVPVY 166 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 121 bits (291), Expect = 9e-29 Identities = 58/124 (46%), Positives = 78/124 (62%), Gaps = 9/124 (7%) Frame = +2 Query: 263 DSYVGDEAQSKRGI-LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP 439 D ++G++A K +L YPI HG + NWD ME+ W + + LR PE+H LLTE P Sbjct: 73 DFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPP 132 Query: 440 LNPKANREKMTXIMFETFNTPAMYVAIQAVLSLYASGRT--------TGIVLDSGDGVSH 595 LNP NRE IMFE+FN +Y+A+QAVL+L AS + TG V+DSGDGV+H Sbjct: 133 LNPPENRENTAEIMFESFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDSGDGVTH 192 Query: 596 TVPI 607 +P+ Sbjct: 193 IIPV 196 Score = 26.6 bits (56), Expect = 2.8 Identities = 10/16 (62%), Positives = 13/16 (81%) Frame = +3 Query: 162 KAGFAGDDAPRAVFPS 209 K G+AG+DAP VFP+ Sbjct: 18 KLGYAGNDAPSYVFPT 33 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 78.2 bits (184), Expect = 9e-16 Identities = 38/122 (31%), Positives = 71/122 (58%), Gaps = 3/122 (2%) Frame = +2 Query: 251 MGQKDSYVGDEAQSKRGILTL-KYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLL 427 + + + VG++ + G ++ + P E +++NWD ME++ +TF +L + EHP+ + Sbjct: 62 LSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICM 120 Query: 428 TEAPLNPKANREKMTXIMFETFNTPAMYVAIQAVLSLYASGR--TTGIVLDSGDGVSHTV 601 TE NP R MT ++FE +N P++ I + S Y + + ++GIVL+ G+ SH + Sbjct: 121 TEPLANPTYVRSTMTELLFELYNAPSVAYGIDGLFSFYHNTKPSSSGIVLNLGNAASHVI 180 Query: 602 PI 607 P+ Sbjct: 181 PV 182 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 76.2 bits (179), Expect = 4e-15 Identities = 38/102 (37%), Positives = 54/102 (52%) Frame = +2 Query: 305 LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTXIMF 484 + +K I +G V NWD +W + +L+ P EHP+L+TE NP NR K MF Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMF 122 Query: 485 ETFNTPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIY 610 E+ PA Y+A Q + +ASG+ T ++D G S IY Sbjct: 123 ESLRCPATYLAKQETCAAFASGKGTACLVDIGAERSSVSAIY 164 Score = 25.8 bits (54), Expect = 4.9 Identities = 9/20 (45%), Positives = 14/20 (70%) Frame = +3 Query: 162 KAGFAGDDAPRAVFPSXRGK 221 + GF+G+D P+ V PS G+ Sbjct: 24 RIGFSGEDIPKCVLPSYCGE 43 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 72.1 bits (169), Expect = 6e-14 Identities = 44/166 (26%), Positives = 84/166 (50%), Gaps = 2/166 (1%) Frame = +2 Query: 116 KKLPRLVVDNGSGYVQGXXXXXXXXXXXVPLXSWEGPAIRXVMVGMGQKDSYVGDEAQSK 295 +++P LV+D GS + + +P S+ G +R + G ++ YV DE Q Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILP--SYYG--VRSDVTG---RNKYVVDELQIH 60 Query: 296 RGI--LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKM 469 I + +K +GI+ +W+ W +L+V P E+ +++TE NP++ R+++ Sbjct: 61 APIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQI 120 Query: 470 TXIMFETFNTPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPI 607 FE + PA Y+ QAV +A+ ++T +++D G + P+ Sbjct: 121 MEAAFEQLHVPAFYLTKQAVCVAFANSKSTALIVDIGSDNASVTPV 166 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 49.2 bits (112), Expect = 5e-07 Identities = 30/100 (30%), Positives = 46/100 (46%), Gaps = 4/100 (4%) Frame = +2 Query: 320 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTXIMFE 487 PI+ G V +W+ ++ W H Y+ L P + +PV L +RE T FE Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171 Query: 488 TFNTPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPI 607 P +A + ++ LYA G G+V+D G + PI Sbjct: 172 ECQVPGFTIAYEPLMGLYAIGILHGLVIDIGYEKTDITPI 211 >SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1666 Score = 31.1 bits (67), Expect = 0.13 Identities = 19/53 (35%), Positives = 30/53 (56%), Gaps = 5/53 (9%) Frame = +2 Query: 380 FYNELRVAPEEHPVLLTE--APLNPKANREKMTXIMFETFNTPAM---YVAIQ 523 +Y L E+HP+LLT+ A L P+ + ++ I ++ NTP + VAIQ Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 27.5 bits (58), Expect = 1.6 Identities = 14/36 (38%), Positives = 17/36 (47%) Frame = +3 Query: 504 PCTSPSKPCSRCTRPVVPPVSCWTPATVSPTPCPST 611 P T+P P T PV P S P+ P P PS+ Sbjct: 1037 PSTAPPVPIPTSTPPV-PKSSSGAPSAPPPVPAPSS 1071 >SPCC757.09c |rnc1||RNA-binding protein that suppresses calcineurin deletion Rnc1|Schizosaccharomyces pombe|chr 3|||Manual Length = 398 Score = 27.1 bits (57), Expect = 2.1 Identities = 13/29 (44%), Positives = 16/29 (55%) Frame = +3 Query: 525 PCSRCTRPVVPPVSCWTPATVSPTPCPST 611 P SR T+P+ S +P VSP PST Sbjct: 262 PVSRLTQPLPSLASTASPQQVSPPAAPST 290 >SPBC21D10.12 |hob1||BAR adaptor protein Hob1|Schizosaccharomyces pombe|chr 2|||Manual Length = 466 Score = 27.1 bits (57), Expect = 2.1 Identities = 12/36 (33%), Positives = 15/36 (41%) Frame = +3 Query: 501 PPCTSPSKPCSRCTRPVVPPVSCWTPATVSPTPCPS 608 PP P + T PV PV+ A P P P+ Sbjct: 367 PPPVMPQPAAAAVTTPVAAPVAAAAAAVPVPPPAPA 402 >SPAC17C9.01c |nuc2|apc3, SPAC1851.01|anaphase-promoting complex subunit Apc3|Schizosaccharomyces pombe|chr 1|||Manual Length = 665 Score = 25.4 bits (53), Expect = 6.5 Identities = 11/31 (35%), Positives = 18/31 (58%) Frame = +2 Query: 2 KLLESVSCFSRLAVTAGRLIFILSHLHLTNF 94 KL E+++CF L + F+L+ L +T F Sbjct: 344 KLREALNCFQSLPIEQQNTPFVLAKLGITYF 374 >SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces pombe|chr 2|||Manual Length = 226 Score = 25.0 bits (52), Expect = 8.6 Identities = 10/25 (40%), Positives = 17/25 (68%) Frame = +2 Query: 422 LLTEAPLNPKANREKMTXIMFETFN 496 +L APL+ + +KM ++FET+N Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205 >SPBC4C3.12 |sep1||fork head transcription factor Sep1|Schizosaccharomyces pombe|chr 2|||Manual Length = 663 Score = 25.0 bits (52), Expect = 8.6 Identities = 12/28 (42%), Positives = 16/28 (57%) Frame = +3 Query: 522 KPCSRCTRPVVPPVSCWTPATVSPTPCP 605 KP S+ TRP +P + T +V P P P Sbjct: 65 KPSSKATRPYIPSYTRLT-YSVPPLPIP 91 >SPBC1683.09c |frp1||ferric-chelate reductase Frp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 564 Score = 25.0 bits (52), Expect = 8.6 Identities = 11/29 (37%), Positives = 17/29 (58%) Frame = +2 Query: 239 VMVGMGQKDSYVGDEAQSKRGILTLKYPI 325 +M+G +Y+G AQ KR +LT + I Sbjct: 165 IMIGAIHGFAYIGLAAQGKRALLTARVTI 193 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,647,111 Number of Sequences: 5004 Number of extensions: 54679 Number of successful extensions: 162 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 135 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 152 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 267622334 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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