BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fprWP01_F_L20 (636 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC18G6.14c |rps7||40S ribosomal protein S7|Schizosaccharomyces... 138 8e-34 SPCC1020.09 |||WD repeat protein, human WDR79 family|Schizosacch... 27 2.3 SPAC13F5.01c |msh1|SPAC23C11.18c|MutS protein homolog 1|Schizosa... 27 2.3 SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|... 27 3.0 SPAC767.01c |vps1|SPAC9G1.14c|dynamin family protein Vps1|Schizo... 27 3.0 SPAC19A8.10 |rfp1|mug140|ubiquitin-protein ligase E3 Rfp1|Schizo... 26 5.2 SPBC14F5.13c |||alkaline phosphatase |Schizosaccharomyces pombe|... 25 9.1 >SPAC18G6.14c |rps7||40S ribosomal protein S7|Schizosaccharomyces pombe|chr 1|||Manual Length = 195 Score = 138 bits (333), Expect = 8e-34 Identities = 72/145 (49%), Positives = 102/145 (70%), Gaps = 6/145 (4%) Frame = +3 Query: 45 LIKAXGAEADSFEXSISQALVELETNS-DLKAQLRELYITKAKEIELHN-KKSIIIYVPM 218 ++K ++ + ++Q L +LE++S D+ +LR L IT A+E+E+ KK+I+++VP Sbjct: 7 IVKRSSSQPTETDLLVAQCLYDLESSSKDMAKELRPLQITSAREVEVGGGKKAIVVFVPQ 66 Query: 219 PKLKAFQKIQIRLVRELEKKFSGKHVVFVGDRKILPKPSHKTRVANKQKRPRSRTLTSVY 398 P LKAF K Q RL RELEKKF+ +HV+F+ R+ILPKP K+RV QKRPRSRTLT+V+ Sbjct: 67 PLLKAFHKCQARLTRELEKKFADRHVIFIAQRRILPKPGRKSRVT--QKRPRSRTLTAVH 124 Query: 399 DAILEDLVFP----G*DRRQAHQGQ 461 +AILED+VFP G RQA G+ Sbjct: 125 NAILEDIVFPTEIIGKRTRQATDGR 149 Score = 65.7 bits (153), Expect = 5e-12 Identities = 29/53 (54%), Positives = 37/53 (69%) Frame = +1 Query: 433 EIVGKRIRVKLDGSQLIKVHLDKNQQTTIEHKVDTFQSVYKKLTGREVTFEFP 591 EI+GKR R DG + IKV LD T+++K+ +F SVY KLTG+ VTFEFP Sbjct: 136 EIIGKRTRQATDGRKTIKVFLDNRDANTVDYKLGSFSSVYHKLTGKNVTFEFP 188 >SPCC1020.09 |||WD repeat protein, human WDR79 family|Schizosaccharomyces pombe|chr 3|||Manual Length = 399 Score = 27.1 bits (57), Expect = 2.3 Identities = 16/27 (59%), Positives = 17/27 (62%) Frame = -3 Query: 334 LGLGRILRSPTKTTCLPLNFFSSSRTS 254 LG I +SPTK PLNFF SSR S Sbjct: 33 LGTNVIAQSPTK----PLNFFHSSRWS 55 >SPAC13F5.01c |msh1|SPAC23C11.18c|MutS protein homolog 1|Schizosaccharomyces pombe|chr 1|||Manual Length = 941 Score = 27.1 bits (57), Expect = 2.3 Identities = 16/56 (28%), Positives = 30/56 (53%), Gaps = 1/56 (1%) Frame = +1 Query: 427 LAEIVGKRIRVKLDGSQLIKVHLDKNQQTTIEHKVDTF-QSVYKKLTGREVTFEFP 591 L++ +GK+ ++ ++L VHL + TIE + F Q+V + T +F+ P Sbjct: 578 LSKRLGKKATLRKSPAKLYYVHLKLSGNETIERFIKKFTQAVLFQSTKSTASFQLP 633 >SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 1184 Score = 26.6 bits (56), Expect = 3.0 Identities = 9/24 (37%), Positives = 18/24 (75%) Frame = +1 Query: 307 ETVRSCLSPATKPVLLTNKRGHAQ 378 E+ + ++ +TKPV +T+K GH++ Sbjct: 1069 ESTKPAVNNSTKPVAVTSKNGHSR 1092 >SPAC767.01c |vps1|SPAC9G1.14c|dynamin family protein Vps1|Schizosaccharomyces pombe|chr 1|||Manual Length = 678 Score = 26.6 bits (56), Expect = 3.0 Identities = 13/31 (41%), Positives = 22/31 (70%) Frame = +3 Query: 204 IYVPMPKLKAFQKIQIRLVRELEKKFSGKHV 296 +++P K F+KI+ +VRE E+K +GK+V Sbjct: 101 LHLPGQKFFEFEKIREEIVRETEEK-TGKNV 130 >SPAC19A8.10 |rfp1|mug140|ubiquitin-protein ligase E3 Rfp1|Schizosaccharomyces pombe|chr 1|||Manual Length = 254 Score = 25.8 bits (54), Expect = 5.2 Identities = 12/36 (33%), Positives = 21/36 (58%) Frame = +3 Query: 324 PKPSHKTRVANKQKRPRSRTLTSVYDAILEDLVFPG 431 P S + R N+++ RSR S + + LED+++ G Sbjct: 49 PVLSPRRRRMNRRRNERSRNFPSNHLSYLEDMIYLG 84 >SPBC14F5.13c |||alkaline phosphatase |Schizosaccharomyces pombe|chr 2|||Manual Length = 532 Score = 25.0 bits (52), Expect = 9.1 Identities = 10/22 (45%), Positives = 13/22 (59%) Frame = -2 Query: 512 VCWFLSKCTLMSCEPSNLTLMR 447 +C FL K T SC NL L++ Sbjct: 207 LCSFLPKSTYRSCRSDNLDLLK 228 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,433,268 Number of Sequences: 5004 Number of extensions: 47870 Number of successful extensions: 137 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 131 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 136 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 283719918 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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