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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fprWP01_F_K11
         (654 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g47510.1 68415.m05930 fumarate hydratase, putative / fumarase...   224   3e-59
At5g50950.2 68418.m06319 fumarate hydratase, putative / fumarase...   223   1e-58
At5g50950.1 68418.m06318 fumarate hydratase, putative / fumarase...   223   1e-58
At3g19390.1 68416.m02459 cysteine proteinase, putative / thiol p...    29   2.0  
At1g13020.1 68414.m01510 eukaryotic translation initiation facto...    29   2.0  
At5g13700.1 68418.m01595 polyamine oxidase, putative similar to ...    28   6.2  
At4g11610.1 68417.m01859 C2 domain-containing protein contains I...    27   8.2  
At2g43610.1 68415.m05421 glycoside hydrolase family 19 protein s...    27   8.2  

>At2g47510.1 68415.m05930 fumarate hydratase, putative / fumarase,
           putative similar to SP|P55250 Fumarate hydratase,
           mitochondrial precursor (EC 4.2.1.2) (Fumarase)
           {Rhizopus oryzae}; contains Pfam profile PF00206: Lyase
          Length = 492

 Score =  224 bits (548), Expect = 3e-59
 Identities = 108/167 (64%), Positives = 128/167 (76%)
 Frame = +1

Query: 154 NISTTSVTARKEKDTFGELDVPDDKLYGAQTVRSVMNFPIGGIEERMPYPVIVAFGILKK 333
           ++ + S + R+E+DTFG + VP DKL+GAQT RS+ NF IGG  ERMP P++ AFG+LKK
Sbjct: 24  SLRSYSTSFREERDTFGPIQVPSDKLWGAQTQRSLQNFEIGGERERMPEPIVRAFGVLKK 83

Query: 334 AAAKVNIEYGLEKKIADAIMQACDDVISGKLYREGHFPLVIWQTGSGTQSNMNTNEVIAN 513
            AAKVN+EYGL+  I  AIMQA  +V  GKL    HFPLV+WQTGSGTQSNMN NEVIAN
Sbjct: 84  CAAKVNMEYGLDPTIGKAIMQAAQEVAEGKL--NDHFPLVVWQTGSGTQSNMNANEVIAN 141

Query: 514 RAIQILGGKLGSKDPVHPNDHVNKSQSSNDTYPTAMHIAVAMELRDR 654
           RA +ILG K G K  VHPNDHVN+SQSSNDT+PT MHIA A E+  R
Sbjct: 142 RAAEILGRKRGEK-CVHPNDHVNRSQSSNDTFPTVMHIAAATEINSR 187


>At5g50950.2 68418.m06319 fumarate hydratase, putative / fumarase,
           putative similar to SP|P55250 Fumarate hydratase,
           mitochondrial precursor (EC 4.2.1.2) (Fumarase)
           {Rhizopus oryzae}; contains Pfam profile PF00206: Lyase
          Length = 499

 Score =  223 bits (544), Expect = 1e-58
 Identities = 107/162 (66%), Positives = 125/162 (77%)
 Frame = +1

Query: 169 SVTARKEKDTFGELDVPDDKLYGAQTVRSVMNFPIGGIEERMPYPVIVAFGILKKAAAKV 348
           S   R+E+DTFG + VP DKL+GAQT RS+ NF IGG  ERMP P++ AFG+LKK AAKV
Sbjct: 36  STPFREERDTFGPIQVPSDKLWGAQTQRSLQNFEIGGDRERMPEPIVRAFGVLKKCAAKV 95

Query: 349 NIEYGLEKKIADAIMQACDDVISGKLYREGHFPLVIWQTGSGTQSNMNTNEVIANRAIQI 528
           N+EYGL+  I +AIM+A  +V  GKL    HFPLV+WQTGSGTQSNMN NEVIANRA +I
Sbjct: 96  NMEYGLDPMIGEAIMEAAQEVAEGKL--NDHFPLVVWQTGSGTQSNMNANEVIANRAAEI 153

Query: 529 LGGKLGSKDPVHPNDHVNKSQSSNDTYPTAMHIAVAMELRDR 654
           LG K G K  VHPNDHVN+SQSSNDT+PT MHIA A E+  R
Sbjct: 154 LGHKRGEK-IVHPNDHVNRSQSSNDTFPTVMHIAAATEITSR 194


>At5g50950.1 68418.m06318 fumarate hydratase, putative / fumarase,
           putative similar to SP|P55250 Fumarate hydratase,
           mitochondrial precursor (EC 4.2.1.2) (Fumarase)
           {Rhizopus oryzae}; contains Pfam profile PF00206: Lyase
          Length = 510

 Score =  223 bits (544), Expect = 1e-58
 Identities = 107/162 (66%), Positives = 125/162 (77%)
 Frame = +1

Query: 169 SVTARKEKDTFGELDVPDDKLYGAQTVRSVMNFPIGGIEERMPYPVIVAFGILKKAAAKV 348
           S   R+E+DTFG + VP DKL+GAQT RS+ NF IGG  ERMP P++ AFG+LKK AAKV
Sbjct: 36  STPFREERDTFGPIQVPSDKLWGAQTQRSLQNFEIGGDRERMPEPIVRAFGVLKKCAAKV 95

Query: 349 NIEYGLEKKIADAIMQACDDVISGKLYREGHFPLVIWQTGSGTQSNMNTNEVIANRAIQI 528
           N+EYGL+  I +AIM+A  +V  GKL    HFPLV+WQTGSGTQSNMN NEVIANRA +I
Sbjct: 96  NMEYGLDPMIGEAIMEAAQEVAEGKL--NDHFPLVVWQTGSGTQSNMNANEVIANRAAEI 153

Query: 529 LGGKLGSKDPVHPNDHVNKSQSSNDTYPTAMHIAVAMELRDR 654
           LG K G K  VHPNDHVN+SQSSNDT+PT MHIA A E+  R
Sbjct: 154 LGHKRGEK-IVHPNDHVNRSQSSNDTFPTVMHIAAATEITSR 194


>At3g19390.1 68416.m02459 cysteine proteinase, putative / thiol
           protease, putative contains similarity to cysteine
           proteinase RD21A (thiol protease) GI:435619, SP:P43297
           from [Arabidopsis thaliana]
          Length = 452

 Score = 29.5 bits (63), Expect = 2.0
 Identities = 14/31 (45%), Positives = 15/31 (48%)
 Frame = -3

Query: 619 WRWGTCRSSSATCSRGRWDAPGPCCPVSRPV 527
           + WG C   SATC     D    CCP S PV
Sbjct: 386 YSWGCCPYESATCC----DDGSSCCPQSYPV 412


>At1g13020.1 68414.m01510 eukaryotic translation initiation factor,
           putative (EIF4B5) eukaryotic initiation factor 4B
           (GI:6739522) {Arabidopsis thaliana}; EST gb|T22808 comes
           from this gene
          Length = 549

 Score = 29.5 bits (63), Expect = 2.0
 Identities = 18/52 (34%), Positives = 23/52 (44%)
 Frame = +3

Query: 432 RGSLPPRHLADWLRYSVQHEHERGDCEPRHTNTGRETGQQGPGASQRPREQV 587
           RG LP        R  +  E  + D E   T T  +T +  P  + RPREQV
Sbjct: 253 RGVLPSGGGVQEERRRLVFEPRKADTEVSETPTAVKTSKPSPFGAARPREQV 304


>At5g13700.1 68418.m01595 polyamine oxidase, putative similar to
           SP|O64411 Polyamine oxidase precursor (EC 1.5.3.11) from
           Zea mays
          Length = 472

 Score = 27.9 bits (59), Expect = 6.2
 Identities = 21/67 (31%), Positives = 36/67 (53%), Gaps = 6/67 (8%)
 Frame = +1

Query: 304 VIVAFGILKKAAAKVNIEYGLEKKIADAIMQACDDVISGKLYRE--GHFPLVI---WQTG 468
           +I+  GI   +AAKV +E G+E    D ++    D I G+++++  G  P+ +   W  G
Sbjct: 7   IIIGAGISGISAAKVLVENGVE----DVLILEATDRIGGRIHKQNFGDVPVELGAGWIAG 62

Query: 469 -SGTQSN 486
             G +SN
Sbjct: 63  VGGKESN 69


>At4g11610.1 68417.m01859 C2 domain-containing protein contains
            INTERPRO:IPR000008 C2 domain
          Length = 1011

 Score = 27.5 bits (58), Expect = 8.2
 Identities = 10/25 (40%), Positives = 16/25 (64%)
 Frame = -2

Query: 395  CMIASAIFFSSPYSILTLAAAFFSI 321
            C IA+ +FF +P  I+   A FF++
Sbjct: 959  CFIAAIVFFITPIQIVVALAGFFTM 983


>At2g43610.1 68415.m05421 glycoside hydrolase family 19 protein
           similar to chitinase GI:17799 from [Brassica napus];
           contains Pfam profiles PF00182: Chitinase class I,
           PF00187: Chitin recognition protein
          Length = 281

 Score = 27.5 bits (58), Expect = 8.2
 Identities = 14/39 (35%), Positives = 17/39 (43%)
 Frame = -3

Query: 625 CAWRWGTCRSSSATCSRGRWDAPGPCCPVSRPVFVWRGS 509
           C  RWG C ++ A C  G     GPC    +P     GS
Sbjct: 41  CCSRWGYCGTTKAYCGTG--CQSGPCNSKPKPTPTPSGS 77


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,334,788
Number of Sequences: 28952
Number of extensions: 311499
Number of successful extensions: 886
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 858
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 880
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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