BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fprWP01_F_J13 (507 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 72 4e-14 SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 57 2e-09 SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 56 3e-09 SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 52 5e-08 SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 47 1e-06 SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 42 5e-05 SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 32 0.057 SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 29 0.30 SPAC29E6.06c ||SPAC30.10c|cysteine-tRNA ligase |Schizosaccharomy... 25 4.9 SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr... 25 6.5 >SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 103 Score = 72.1 bits (169), Expect = 4e-14 Identities = 34/90 (37%), Positives = 51/90 (56%) Frame = +2 Query: 119 IKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAEMXXXXXXXXXXXXXXX 298 + DS + K+ + + DKLVV+DF ATWCGPCK I PK ++ + Sbjct: 5 VSDSSEFKSIVCQ--DKLVVVDFFATWCGPCKAIAPKFEQF-SNTYSDATFIKVDVDQLS 61 Query: 299 XXASEYNINSMPTFVFVKNGKKLDEFSGAN 388 A+E +++MP+F KNG+K++E GAN Sbjct: 62 EIAAEAGVHAMPSFFLYKNGEKIEEIVGAN 91 >SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccharomyces pombe|chr 2|||Manual Length = 121 Score = 56.8 bits (131), Expect = 2e-09 Identities = 28/91 (30%), Positives = 47/91 (51%) Frame = +2 Query: 119 IKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAEMXXXXXXXXXXXXXXX 298 ++ D TR++ DK+ V+DF A WCGPCK + P L+++ +E Sbjct: 22 VESFGDYNTRIS--ADKVTVVDFYADWCGPCKYLKPFLEKL-SEQNQKASFIAVNADKFS 78 Query: 299 XXASEYNINSMPTFVFVKNGKKLDEFSGANV 391 A + + ++PT V + G++LD GA+V Sbjct: 79 DIAQKNGVYALPTMVLFRKGQELDRIVGADV 109 >SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosaccharomyces pombe|chr 2|||Manual Length = 290 Score = 56.0 bits (129), Expect = 3e-09 Identities = 26/73 (35%), Positives = 38/73 (52%), Gaps = 1/73 (1%) Frame = +2 Query: 173 VVIDFMATWCGPCKMIGPKLDEIAAEMXXXXXXXXXXXXXXXXX-ASEYNINSMPTFVFV 349 + +D A WCGPCK I P ++A++ AS + +MPTFVF Sbjct: 22 LAVDCYADWCGPCKAISPLFSQLASKYASPKFVFAKVNVDEQRQIASGLGVKAMPTFVFF 81 Query: 350 KNGKKLDEFSGAN 388 +NGK++D +GAN Sbjct: 82 ENGKQIDMLTGAN 94 >SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces pombe|chr 1|||Manual Length = 492 Score = 52.0 bits (119), Expect = 5e-08 Identities = 24/74 (32%), Positives = 37/74 (50%), Gaps = 1/74 (1%) Frame = +2 Query: 164 DKLVVIDFMATWCGPCKMIGPKLDEIAAEM-XXXXXXXXXXXXXXXXXASEYNINSMPTF 340 DK++++ F A WCG CK + P+ + A E+ SEY+I PT Sbjct: 39 DKVLMVKFYAPWCGHCKALAPEYESAADELEKDGISLVEVDCTEEGDLCSEYSIRGYPTL 98 Query: 341 VFVKNGKKLDEFSG 382 KNGK++ ++SG Sbjct: 99 NVFKNGKQISQYSG 112 Score = 36.7 bits (81), Expect = 0.002 Identities = 19/68 (27%), Positives = 31/68 (45%) Frame = +2 Query: 167 KLVVIDFMATWCGPCKMIGPKLDEIAAEMXXXXXXXXXXXXXXXXXASEYNINSMPTFVF 346 K V+++F A WCG CK + P +++A E S +I+ PT +F Sbjct: 374 KDVLVEFYAPWCGHCKNLAPTYEKLAEEYSDDSNVVVAKIDATENDIS-VSISGFPTIMF 432 Query: 347 VKNGKKLD 370 K K++ Sbjct: 433 FKANDKVN 440 >SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr 2|||Manual Length = 244 Score = 47.2 bits (107), Expect = 1e-06 Identities = 23/94 (24%), Positives = 42/94 (44%) Frame = +2 Query: 107 MSIHIKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAEMXXXXXXXXXXX 286 MS+ I + + L +++++++F A W PCK + D+ A + Sbjct: 1 MSVEITFVEQFQEILQNGKEQIILLNFYAPWAAPCKQMNQVFDQFAKD-TKNAVFLKIEA 59 Query: 287 XXXXXXASEYNINSMPTFVFVKNGKKLDEFSGAN 388 A +++N++P FV + K L SGAN Sbjct: 60 EKFSDIAESFDVNAVPLFVLIHGAKVLARISGAN 93 >SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces pombe|chr 1|||Manual Length = 359 Score = 41.9 bits (94), Expect = 5e-05 Identities = 25/94 (26%), Positives = 44/94 (46%), Gaps = 3/94 (3%) Frame = +2 Query: 113 IHIKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAA--EMXXXXXXXXXXX 286 + ++ ++L+ + A K +I+F ATWCG CK + P +E+ A E Sbjct: 23 VELQSLNELENTI-RASKKGALIEFYATWCGHCKSLAPVYEELGALFEDHNDVLIGKIDA 81 Query: 287 XXXXXXASEYNINSMPTFV-FVKNGKKLDEFSGA 385 A +Y+I PT + F +G + ++S A Sbjct: 82 DTHSDVADKYHITGFPTLIWFPPDGSEPVQYSNA 115 Score = 30.3 bits (65), Expect = 0.17 Identities = 10/25 (40%), Positives = 16/25 (64%) Frame = +2 Query: 167 KLVVIDFMATWCGPCKMIGPKLDEI 241 K V+++F A WCG CK + P + + Sbjct: 159 KDVLVEFYADWCGYCKRLAPTYETL 183 >SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 363 Score = 31.9 bits (69), Expect = 0.057 Identities = 22/90 (24%), Positives = 34/90 (37%), Gaps = 2/90 (2%) Frame = +2 Query: 125 DSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAEMXXXXXXXXXXXXXXXXX 304 +S + + + G LVV F A WCG CK + P ++A+ + Sbjct: 37 NSKNFRKFVKAKGPSLVV--FYAPWCGYCKKLVPTYQKLASNLHSLLPVTAVDCDADQNR 94 Query: 305 A--SEYNINSMPTFVFVKNGKKLDEFSGAN 388 A S+Y + PT V K S + Sbjct: 95 AVCSQYQVQGFPTIKLVYPSSKGSSLSSTD 124 >SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces pombe|chr 2|||Manual Length = 726 Score = 29.5 bits (63), Expect = 0.30 Identities = 18/61 (29%), Positives = 26/61 (42%), Gaps = 3/61 (4%) Frame = +2 Query: 200 CGPCKMIGPKLD---EIAAEMXXXXXXXXXXXXXXXXXASEYNINSMPTFVFVKNGKKLD 370 CG CK +GP D E A E +S NI ++PT +NG+ ++ Sbjct: 54 CGACKRLGPMWDNMVEKAKEQVEGSNFHFGEVDCSKELSSCANIRAVPTLYLYQNGEIVE 113 Query: 371 E 373 E Sbjct: 114 E 114 >SPAC29E6.06c ||SPAC30.10c|cysteine-tRNA ligase |Schizosaccharomyces pombe|chr 1|||Manual Length = 754 Score = 25.4 bits (53), Expect = 4.9 Identities = 11/44 (25%), Positives = 24/44 (54%), Gaps = 2/44 (4%) Frame = -3 Query: 130 GIFDVNGHLGQ--DKLKLKIISQFKMQIRNTQVTTYSQFGAKLI 5 G+ GH+ + DKL+++++ +F+ +NT + F L+ Sbjct: 477 GVSANGGHVPEKFDKLEIELLEKFQQTQQNTHLALCDSFNTPLV 520 >SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr 2|||Manual Length = 905 Score = 25.0 bits (52), Expect = 6.5 Identities = 14/41 (34%), Positives = 22/41 (53%) Frame = +2 Query: 125 DSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAA 247 + D + RL EAG + +A CGPC + + D+IA+ Sbjct: 431 ERDGITERLEEAGATV-----LANACGPCIGMWKRTDDIAS 466 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,701,974 Number of Sequences: 5004 Number of extensions: 28233 Number of successful extensions: 90 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 80 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 85 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 202220600 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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