BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fprWP01_F_E05
(546 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g74270.1 68414.m08601 60S ribosomal protein L35a (RPL35aC) si... 113 5e-26
At1g07070.1 68414.m00753 60S ribosomal protein L35a (RPL35aA) si... 113 5e-26
At1g41880.1 68414.m04836 60S ribosomal protein L35a (RPL35aB) id... 112 2e-25
At3g55750.1 68416.m06194 60S ribosomal protein L35a (RPL35aD) ri... 111 2e-25
At4g11860.1 68417.m01887 expressed protein contains Pfam domain ... 29 1.5
At2g04350.2 68415.m00434 long-chain-fatty-acid--CoA ligase famil... 28 3.5
At2g04350.1 68415.m00433 long-chain-fatty-acid--CoA ligase famil... 28 3.5
At4g21130.1 68417.m03055 transducin family protein / WD-40 repea... 27 6.2
At3g05180.1 68416.m00565 GDSL-motif lipase/hydrolase family prot... 27 6.2
At5g51195.1 68418.m06348 hypothetical protein 27 8.2
>At1g74270.1 68414.m08601 60S ribosomal protein L35a (RPL35aC)
similar to ribosomal protein L33B GB:NP_014877 from
[Saccharomyces cerevisiae]
Length = 112
Score = 113 bits (273), Expect = 5e-26
Identities = 53/110 (48%), Positives = 70/110 (63%)
Frame = +3
Query: 177 RLYAKAVFTGYKRGLRNQHENTALLKVEGAKDRNDAVFYAGKHCVYVYRAKKRTPIPGGP 356
RLY + GYKR NQ+ NT+L+++EG + + +Y GK Y+Y+AK +
Sbjct: 11 RLYVRGTILGYKRSKSNQYPNTSLVQIEGVNTQEEVNWYKGKRMAYIYKAKTKK------ 64
Query: 357 RGKKTKLRAIWGKVTRPHGNSGSVRAKFKSNLPAQAMGHRIRVMLYPSRI 506
+ R IWGKVTRPHGNSG VRAKF SNLP ++MG R+RV +YPS I
Sbjct: 65 --NGSHYRCIWGKVTRPHGNSGVVRAKFTSNLPPKSMGSRVRVFMYPSNI 112
>At1g07070.1 68414.m00753 60S ribosomal protein L35a (RPL35aA)
similar to ribosomal protein L35a GI:57118 from [Rattus
norvegicus]
Length = 112
Score = 113 bits (273), Expect = 5e-26
Identities = 54/110 (49%), Positives = 69/110 (62%)
Frame = +3
Query: 177 RLYAKAVFTGYKRGLRNQHENTALLKVEGAKDRNDAVFYAGKHCVYVYRAKKRTPIPGGP 356
RLY + GYKR NQ+ NT+L++VEG + +Y GK Y+Y+AK +
Sbjct: 11 RLYVRGTILGYKRSKSNQYPNTSLVQVEGVNTTEEVSWYKGKRMAYIYKAKTKK------ 64
Query: 357 RGKKTKLRAIWGKVTRPHGNSGSVRAKFKSNLPAQAMGHRIRVMLYPSRI 506
+ R IWGKVTRPHGNSG VRAKF SNLP ++MG R+RV +YPS I
Sbjct: 65 --NGSHYRCIWGKVTRPHGNSGVVRAKFTSNLPPKSMGSRVRVFMYPSNI 112
>At1g41880.1 68414.m04836 60S ribosomal protein L35a (RPL35aB)
identical to GB:CAB81600 from [Arabidopsis thaliana]
Length = 111
Score = 112 bits (269), Expect = 2e-25
Identities = 52/110 (47%), Positives = 70/110 (63%)
Frame = +3
Query: 177 RLYAKAVFTGYKRGLRNQHENTALLKVEGAKDRNDAVFYAGKHCVYVYRAKKRTPIPGGP 356
RLY + GYKR NQ+ NT+L+++EG + + +Y GK Y+Y+AK +
Sbjct: 10 RLYVRGTVLGYKRSKSNQYPNTSLIQIEGVNTQEEVNWYKGKRLAYIYKAKTKK------ 63
Query: 357 RGKKTKLRAIWGKVTRPHGNSGSVRAKFKSNLPAQAMGHRIRVMLYPSRI 506
+ R IWGKVTRPHGNSG VR+KF SNLP ++MG R+RV +YPS I
Sbjct: 64 --NGSHYRCIWGKVTRPHGNSGVVRSKFTSNLPPKSMGARVRVFMYPSNI 111
>At3g55750.1 68416.m06194 60S ribosomal protein L35a (RPL35aD)
ribosomal protein L35a.e.c15, Saccharomyces cerevisiae,
PIR:S44069
Length = 111
Score = 111 bits (268), Expect = 2e-25
Identities = 52/110 (47%), Positives = 70/110 (63%)
Frame = +3
Query: 177 RLYAKAVFTGYKRGLRNQHENTALLKVEGAKDRNDAVFYAGKHCVYVYRAKKRTPIPGGP 356
RLY + GYKR NQ+ NT+L+++EG + + +Y GK Y+Y+AK +
Sbjct: 10 RLYVRGTVLGYKRSKSNQYPNTSLVQIEGVNTQEEVNWYKGKRLAYIYKAKTKK------ 63
Query: 357 RGKKTKLRAIWGKVTRPHGNSGSVRAKFKSNLPAQAMGHRIRVMLYPSRI 506
+ R IWGKVTRPHGNSG VR+KF SNLP ++MG R+RV +YPS I
Sbjct: 64 --NGSHYRCIWGKVTRPHGNSGVVRSKFTSNLPPKSMGARVRVFMYPSNI 111
>At4g11860.1 68417.m01887 expressed protein contains Pfam domain
PF04424: Protein of unknown function (DUF544)
Length = 682
Score = 29.5 bits (63), Expect = 1.5
Identities = 14/62 (22%), Positives = 29/62 (46%)
Frame = +3
Query: 258 EGAKDRNDAVFYAGKHCVYVYRAKKRTPIPGGPRGKKTKLRAIWGKVTRPHGNSGSVRAK 437
EG K+R VF+ H +++ + + +G + +W K+ +G++ + A
Sbjct: 508 EGLKERELCVFFRNNHFCTMFKYEGELYLLATDQGYLNQPDLVWEKLNEVNGDTAFMTAT 567
Query: 438 FK 443
FK
Sbjct: 568 FK 569
>At2g04350.2 68415.m00434 long-chain-fatty-acid--CoA ligase family
protein / long-chain acyl-CoA synthetase family protein
(LACS8) similar to LACS 4 [SP|O35547] from Rattus
norvegicus, LACS 4 [SP|O60488] from Homo sapiens;
contains Pfam HMM hit: AMP-binding enzymes PF00501
Length = 720
Score = 28.3 bits (60), Expect = 3.5
Identities = 16/47 (34%), Positives = 23/47 (48%)
Frame = -3
Query: 532 FL*LAEGS*ILEGYSITRILCPIAWAGRLDLNLARTLPELPCGRVTL 392
F+ + GS I +GY +T ++ D + R P LPCG V L
Sbjct: 463 FINICMGSPIGQGYGLTETCAGATFSEWDDPAVGRVGPPLPCGYVKL 509
>At2g04350.1 68415.m00433 long-chain-fatty-acid--CoA ligase family
protein / long-chain acyl-CoA synthetase family protein
(LACS8) similar to LACS 4 [SP|O35547] from Rattus
norvegicus, LACS 4 [SP|O60488] from Homo sapiens;
contains Pfam HMM hit: AMP-binding enzymes PF00501
Length = 720
Score = 28.3 bits (60), Expect = 3.5
Identities = 16/47 (34%), Positives = 23/47 (48%)
Frame = -3
Query: 532 FL*LAEGS*ILEGYSITRILCPIAWAGRLDLNLARTLPELPCGRVTL 392
F+ + GS I +GY +T ++ D + R P LPCG V L
Sbjct: 463 FINICMGSPIGQGYGLTETCAGATFSEWDDPAVGRVGPPLPCGYVKL 509
>At4g21130.1 68417.m03055 transducin family protein / WD-40 repeat
family protein contains 6 WD-40 repeats (PF00400); some
similarity to a group of proteins with homology to
mammalian apoptosis regulators identified in zebrafish
(PUBMED:10917738)Apaf-1(gi:7677507)
Length = 537
Score = 27.5 bits (58), Expect = 6.2
Identities = 18/57 (31%), Positives = 30/57 (52%), Gaps = 1/57 (1%)
Frame = -2
Query: 488 HHSYSVS-HSLGREVRLELGSDTARVAMWAGHLAPDSTQLGFFATGTSGNWCPLLSS 321
H S +S +LGRE L +G D G + P+ST+L + A+ ++ C ++S
Sbjct: 304 HQSELLSIDALGRERVLSVGRDRTMQLYKVGIVVPESTRLIYRASESNFECCCFVNS 360
>At3g05180.1 68416.m00565 GDSL-motif lipase/hydrolase family protein
similar to early nodulin ENOD8 [Medicago sativa]
GI:304037, elicitor-induced glycoprotein iEP4 [Daucus
carota] GI:1911765, lanatoside 15'-O-acetylesterase
[Digitalis lanata] GI:3688284; contains InterPro Entry
IPR001087 Lipolytic enzyme, G-D-S-L family
Length = 379
Score = 27.5 bits (58), Expect = 6.2
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = -2
Query: 431 SDTARVAMWAGHLAPDSTQLGFFATGTSGNWC 336
SDT ++ G L S ++ FF + TSG +C
Sbjct: 45 SDTGELSSGLGFLPQPSYEITFFRSPTSGRFC 76
>At5g51195.1 68418.m06348 hypothetical protein
Length = 267
Score = 27.1 bits (57), Expect = 8.2
Identities = 13/39 (33%), Positives = 18/39 (46%)
Frame = -2
Query: 452 EVRLELGSDTARVAMWAGHLAPDSTQLGFFATGTSGNWC 336
E++ E+ S+ W D T FFA+ SG WC
Sbjct: 209 EIQAEMESEDVAATFWID----DETHDDFFASSKSGWWC 243
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,526,628
Number of Sequences: 28952
Number of extensions: 241486
Number of successful extensions: 648
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 616
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 644
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1023490624
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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