BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fprWP01_F_E02 (609 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC576.07 |ret3||coatomer zeta subunit |Schizosaccharomyces pom... 105 7e-24 SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4 |Schizosac... 41 1e-04 SPBC685.04c |aps2||AP-2 adaptor complex subunit Aps2 |Schizosacc... 31 0.100 SPAP27G11.06c |||AP-1 adaptor complex subunit |Schizosaccharomyc... 29 0.70 SPCC16C4.02c |||DUF1941 family protein|Schizosaccharomyces pombe... 26 3.7 SPCC1827.01c |||DUF1253 family protein|Schizosaccharomyces pombe... 25 6.5 SPCC74.01 |sly1||SNARE binding protein Sly1|Schizosaccharomyces ... 25 8.7 SPBC1703.14c |top1||DNA topoisomerase I|Schizosaccharomyces pomb... 25 8.7 >SPCC576.07 |ret3||coatomer zeta subunit |Schizosaccharomyces pombe|chr 3|||Manual Length = 190 Score = 105 bits (251), Expect = 7e-24 Identities = 54/146 (36%), Positives = 82/146 (56%), Gaps = 8/146 (5%) Frame = +2 Query: 122 TLYIVKGMCILDYEGNRILAKYY--------DKDVLPTTKEQKAFEKNLFNKTHRANAEI 277 TLY V ILD G RI KYY + V + KE+K FEK LF KT + +I Sbjct: 4 TLYAVNAFLILDSSGKRIFTKYYAPPHLKEGEGGVFNSVKEEKTFEKGLFEKTWKTQNDI 63 Query: 278 IMLDGLTCVYKSNVDLFFYVMGSSHENELILQSVLNALYESVSLLLRRNMERRVLMENLD 457 + DG V + +D+ FY++G ENE++L L ++ +++ LL + ++R L+EN D Sbjct: 64 LTYDGKLVVMLTVMDVIFYIVGGMEENEVMLYECLRSIRDALELLFKYVPDKRTLLENYD 123 Query: 458 AVMLAFDEICDGGVILDADPTSIVSR 535 +++ DE D GVIL+ +P I +R Sbjct: 124 QLVIVVDETIDDGVILETEPALIAAR 149 >SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4 |Schizosaccharomyces pombe|chr 1|||Manual Length = 446 Score = 41.1 bits (92), Expect = 1e-04 Identities = 26/138 (18%), Positives = 63/138 (45%) Frame = +2 Query: 131 IVKGMCILDYEGNRILAKYYDKDVLPTTKEQKAFEKNLFNKTHRANAEIIMLDGLTCVYK 310 ++ G+ I + +G+ ++ K + D+ + E F + T + I+ + T +Y Sbjct: 1 MISGLFIFNLKGDTLICKTFRHDLKKSVTE--IFRVAILTNTDYRHP-IVSIGSSTYIYT 57 Query: 311 SNVDLFFYVMGSSHENELILQSVLNALYESVSLLLRRNMERRVLMENLDAVMLAFDEICD 490 + DL+ + + N +I+ L +L + ++ + E V +N+ + DE+ D Sbjct: 58 KHEDLYVVAITKGNPNVMIVLEFLESLIQDLTHYFGKLNENTV-KDNVSFIFELLDEMID 116 Query: 491 GGVILDADPTSIVSRAAL 544 G+I +P ++ ++ Sbjct: 117 YGIIQTTEPDALARSVSI 134 >SPBC685.04c |aps2||AP-2 adaptor complex subunit Aps2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 143 Score = 31.5 bits (68), Expect = 0.100 Identities = 28/136 (20%), Positives = 58/136 (42%), Gaps = 1/136 (0%) Frame = +2 Query: 131 IVKGMCILDYEGNRILAKYYDK-DVLPTTKEQKAFEKNLFNKTHRANAEIIMLDGLTCVY 307 +++ + I + G L+KYY D + + + + + + A + + VY Sbjct: 1 MIQFILIQNRHGKNRLSKYYVPFDDDEKVRLKARIHQLISQRNQKFQANFLEWENSKLVY 60 Query: 308 KSNVDLFFYVMGSSHENELILQSVLNALYESVSLLLRRNMERRVLMENLDAVMLAFDEIC 487 + L+F S +N+L + +++ E + N+ L+ N V DEI Sbjct: 61 RRYAGLYFCFCVDSTDNDLAILEMIHFFVEILDSFF-GNVCELDLIFNFYKVSAILDEII 119 Query: 488 DGGVILDADPTSIVSR 535 GG I +++ S++ R Sbjct: 120 LGGEIGESNKKSVLER 135 >SPAP27G11.06c |||AP-1 adaptor complex subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 162 Score = 28.7 bits (61), Expect = 0.70 Identities = 19/81 (23%), Positives = 36/81 (44%) Frame = +2 Query: 290 GLTCVYKSNVDLFFYVMGSSHENELILQSVLNALYESVSLLLRRNMERRVLMENLDAVML 469 G VY+ LFF +NELI+ V++ E + N+ L+ N + Sbjct: 55 GEKIVYRRYASLFFVCGIEQDDNELIILEVIHKFVECLDKYF-GNVCELDLIFNFEKAYY 113 Query: 470 AFDEICDGGVILDADPTSIVS 532 +E+ G + ++ T+++S Sbjct: 114 VMEELLLAGELQESSKTNVLS 134 >SPCC16C4.02c |||DUF1941 family protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 548 Score = 26.2 bits (55), Expect = 3.7 Identities = 9/21 (42%), Positives = 13/21 (61%) Frame = +3 Query: 423 TWSGGFSWRTWMLLCLPSMRS 485 +WS G + W+ + LPSM S Sbjct: 437 SWSNGIDYAKWISVALPSMLS 457 >SPCC1827.01c |||DUF1253 family protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 652 Score = 25.4 bits (53), Expect = 6.5 Identities = 20/78 (25%), Positives = 34/78 (43%), Gaps = 13/78 (16%) Frame = +2 Query: 221 QKAFEKNLFNKTHRANAEIIMLDGLTCVYKS-------------NVDLFFYVMGSSHENE 361 Q F N+ K+H+ ++ L L V+K+ N +L F G + Sbjct: 201 QDVFFTNMSMKSHKLGTSLLALHALNHVFKTRDRVLKNSARISQNPELEFRDQGYTRPKV 260 Query: 362 LILQSVLNALYESVSLLL 415 LIL N+ +E ++LL+ Sbjct: 261 LILLPTRNSAFEFINLLI 278 >SPCC74.01 |sly1||SNARE binding protein Sly1|Schizosaccharomyces pombe|chr 3|||Manual Length = 639 Score = 25.0 bits (52), Expect = 8.7 Identities = 11/44 (25%), Positives = 22/44 (50%) Frame = +2 Query: 311 SNVDLFFYVMGSSHENELILQSVLNALYESVSLLLRRNMERRVL 442 ++V ++V + ELI++ + LYES + + R +L Sbjct: 87 ADVPAIYFVQPTQENIELIIEDLSKGLYESAYVCFSSTISRALL 130 >SPBC1703.14c |top1||DNA topoisomerase I|Schizosaccharomyces pombe|chr 2|||Manual Length = 814 Score = 25.0 bits (52), Expect = 8.7 Identities = 10/35 (28%), Positives = 21/35 (60%) Frame = +2 Query: 191 DKDVLPTTKEQKAFEKNLFNKTHRANAEIIMLDGL 295 D+D +P K + + +KN+ N + + A+++ GL Sbjct: 55 DEDDIPIRKRRASSKKNMSNSSSKKRAKVMGNGGL 89 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,473,042 Number of Sequences: 5004 Number of extensions: 47733 Number of successful extensions: 127 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 125 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 126 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 268287866 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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