Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fprWP01_F_D21
(656 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g31740.1 68415.m03876 expressed protein 123 1e-28
At3g60910.1 68416.m06814 expressed protein low similarity to PIR... 95 3e-20
At3g17365.1 68416.m02219 expressed protein low similarity to PIR... 91 8e-19
At4g34360.1 68417.m04882 protease-related similar to PIR|I46078 ... 83 2e-16
At1g66680.1 68414.m07579 S locus-linked protein, putative simila... 47 1e-05
At1g73600.2 68414.m08520 phosphoethanolamine N-methyltransferase... 38 0.006
At1g73600.1 68414.m08521 phosphoethanolamine N-methyltransferase... 38 0.006
At5g57300.1 68418.m07158 UbiE/COQ5 methyltransferase family prot... 33 0.22
At3g18000.1 68416.m02288 phosphoethanolamine N-methyltransferase... 30 1.6
At1g48600.2 68414.m05434 phosphoethanolamine N-methyltransferase... 29 2.7
At1g48600.1 68414.m05435 phosphoethanolamine N-methyltransferase... 29 2.7
At4g20470.1 68417.m02986 hypothetical protein 29 3.6
At5g43890.1 68418.m05367 flavin-containing monooxygenase family ... 28 6.3
At4g19440.1 68417.m02860 pentatricopeptide (PPR) repeat-containi... 28 6.3
At3g48290.1 68416.m05270 cytochrome P450, putative very strong s... 28 6.3
At2g34030.1 68415.m04166 calcium-binding EF hand family protein ... 27 8.3
>At2g31740.1 68415.m03876 expressed protein
Length = 760
Score = 123 bits (296), Expect = 1e-28
Identities = 69/155 (44%), Positives = 95/155 (61%), Gaps = 9/155 (5%)
Frame = +3
Query: 180 RGNK-AFEWYGEYLELCGQLNKYIKTKDS-------ILITGCGNSSLSADLYDVGYVNIT 335
RGN +FEWY E+ +L L ++ S IL+ GCGNS L+ LYD G+ +IT
Sbjct: 36 RGNDDSFEWYAEWPQLHDSLLPLLQDSSSSSSDSLQILVPGCGNSRLTEHLYDAGFRDIT 95
Query: 336 NVDVSEVVIKQMKNVN-KARTNMKFMCMDATRMSFEDEAFNVVLDKGTLDALMPDATEET 512
NVD S+VVI M N + R +++ MD T+M DE+F+ VLDKG LDALM E
Sbjct: 96 NVDFSKVVISDMLRRNIRTRPELRWRVMDITKMQLADESFDTVLDKGALDALM--EPEVG 153
Query: 513 NAIIDKYFSEIXRVLXLGGRFVCISLLQSHILAKL 617
+ ++Y SE RVL GG+F+C++L +SH+LA L
Sbjct: 154 TKLGNQYLSEAKRVLKPGGKFICLTLAESHVLALL 188
>At3g60910.1 68416.m06814 expressed protein low similarity to
PIR|I46078 endothelin converting enzyme from Bos
primigenius taurus
Length = 252
Score = 95.5 bits (227), Expect = 3e-20
Identities = 48/133 (36%), Positives = 78/133 (58%), Gaps = 1/133 (0%)
Frame = +3
Query: 192 AFEWYGEYLELCGQLNKYIKTKDSILITGCGNSSLSADLYDVGYVNITNVDVSEVVIKQM 371
+F+WY Y L + ++ T +L+ GCGNS +S D+ GY +I NVD+S V I+ M
Sbjct: 29 SFDWYQCYSSLRPFVRSFVSTSSRVLMVGCGNSLMSEDMVKDGYEDIMNVDISSVAIEMM 88
Query: 372 KNVNKARTNMKFMCMDATRMS-FEDEAFNVVLDKGTLDALMPDATEETNAIIDKYFSEIX 548
+ + +K+M MD MS FED++F+ ++DKGTLD+LM + +A + E+
Sbjct: 89 QTKYASVPQLKYMQMDVRDMSYFEDDSFDTIIDKGTLDSLMCGSDALLSA--SRMLGEVS 146
Query: 549 RVLXLGGRFVCIS 587
R++ GG + I+
Sbjct: 147 RLIKPGGTYFLIT 159
>At3g17365.1 68416.m02219 expressed protein low similarity to
PIR|I46078 endothelin converting enzyme from Bos
primigenius taurus
Length = 239
Score = 90.6 bits (215), Expect = 8e-19
Identities = 50/158 (31%), Positives = 85/158 (53%), Gaps = 2/158 (1%)
Frame = +3
Query: 177 KRGNKAFEWYGEYLELCGQLNKYIKTKDS-ILITGCGNSSLSADLYDVGYVNITNVDVSE 353
K ++ F+WY +Y L +N Y+ ++ +L+ GCGNS+ S + D GY ++ ++D+S
Sbjct: 21 KNESEPFDWYQKYSPLAPLINLYVPQRNQRVLVIGCGNSAFSEGMVDDGYEDVVSIDISS 80
Query: 354 VVIKQMKNVNKARTNMKFMCMDATRM-SFEDEAFNVVLDKGTLDALMPDATEETNAIIDK 530
VVI M R +K++ MD M +FED +F+ V+DKGTLD+++ + +
Sbjct: 81 VVIDTMIKKYSDRPQLKYLKMDVRDMKAFEDASFDAVIDKGTLDSIL--CGSNSRQYSTQ 138
Query: 531 YFSEIXRVLXLGGRFVCISLLQSHILAKLMEGXCEKSW 644
E+ RVL G ++ I+ +L + C SW
Sbjct: 139 MLEEVWRVLKDKGVYILITYGAPIYRLRLFKESC--SW 174
>At4g34360.1 68417.m04882 protease-related similar to PIR|I46078
endothelin converting enzyme, Bos primigenius taurus
Length = 248
Score = 83.0 bits (196), Expect = 2e-16
Identities = 49/151 (32%), Positives = 80/151 (52%), Gaps = 8/151 (5%)
Frame = +3
Query: 195 FEWYGEYLELCGQLNKYIKTKDSILITGCGNSSLSADLYDVGYVNITNVDVSEVVIKQMK 374
+EW+ +Y + IK S+L GCGNS L +LY G V+IT +D+S V +++M+
Sbjct: 31 YEWFKDYSHFQHLIISNIKPSSSVLELGCGNSQLCEELYKDGIVDITCIDLSSVAVEKMQ 90
Query: 375 N--VNKARTNMKFMCMDATRMSFEDEAFNVVLDKGTLDALMPDATE------ETNAIIDK 530
+ + K +K + D + F+ E+F+VV++KGT+D L DA + ET + +
Sbjct: 91 SRLLPKGYKEIKVVQADMLDLPFDSESFDVVIEKGTMDVLFVDAGDPWNPRPETVSKVMA 150
Query: 531 YFSEIXRVLXLGGRFVCISLLQSHILAKLME 623
+ RVL G F+ I+ Q H L +
Sbjct: 151 TLDGVHRVLKPDGIFISITFGQPHFRRPLFK 181
>At1g66680.1 68414.m07579 S locus-linked protein, putative similar
to S locus-linked protein SLL2 [Brassica napus]
GI:1518113
Length = 358
Score = 46.8 bits (106), Expect = 1e-05
Identities = 36/137 (26%), Positives = 69/137 (50%), Gaps = 2/137 (1%)
Frame = +3
Query: 219 ELCGQLNKYIKTKDSILITGCGNSSLSADLYDVGYVNITNVDVSEVVIKQMKNVNKAR-- 392
E+ Q +KY+ + + +L G GN L L G+ ++T D S+ ++ +++++
Sbjct: 159 EVNDQADKYLSSWN-VLDLGTGNGLLLHQLAKEGFSDLTGTDYSDGAVELAQHLSQRDGF 217
Query: 393 TNMKFMCMDATRMSFEDEAFNVVLDKGTLDALMPDATEETNAIIDKYFSEIXRVLXLGGR 572
N++FM D E + F +V+DKGTLDA+ ++ Y+ + +++ GG
Sbjct: 218 PNIRFMVDDILDTKLEQQ-FKLVMDKGTLDAIGLHPDGPVKRVM--YWDSVSKLVAPGGI 274
Query: 573 FVCISLLQSHILAKLME 623
V S +H +L+E
Sbjct: 275 LVITSC--NHTKDELVE 289
>At1g73600.2 68414.m08520 phosphoethanolamine N-methyltransferase 3,
putative (NMT3) strong similarity to SP|Q9FR44
Phosphoethanolamine N-methyltransferase 1 (EC 2.1.1.103)
(PEAMT 1) (AtNMT1) {Arabidopsis thaliana}; identical to
SP|Q9C6B9 Putative phosphoethanolamine
N-methyltransferase 3 {Arabidopsis thaliana}
Length = 376
Score = 37.9 bits (84), Expect = 0.006
Identities = 20/59 (33%), Positives = 32/59 (54%), Gaps = 2/59 (3%)
Frame = +3
Query: 339 VDVSEVVIKQMKNVNKARTNMKFMCMDAT--RMSFEDEAFNVVLDKGTLDALMPDATEE 509
VD E VIK+ +N+N N+KF+C D T M+F +E+ +++ L L E+
Sbjct: 94 VDFIESVIKKNENINGHYKNVKFLCADVTSPNMNFPNESMDLIFSNWLLMYLSDQEVED 152
>At1g73600.1 68414.m08521 phosphoethanolamine N-methyltransferase 3,
putative (NMT3) strong similarity to SP|Q9FR44
Phosphoethanolamine N-methyltransferase 1 (EC 2.1.1.103)
(PEAMT 1) (AtNMT1) {Arabidopsis thaliana}; identical to
SP|Q9C6B9 Putative phosphoethanolamine
N-methyltransferase 3 {Arabidopsis thaliana}
Length = 555
Score = 37.9 bits (84), Expect = 0.006
Identities = 20/59 (33%), Positives = 32/59 (54%), Gaps = 2/59 (3%)
Frame = +3
Query: 339 VDVSEVVIKQMKNVNKARTNMKFMCMDAT--RMSFEDEAFNVVLDKGTLDALMPDATEE 509
VD E VIK+ +N+N N+KF+C D T M+F +E+ +++ L L E+
Sbjct: 145 VDFIESVIKKNENINGHYKNVKFLCADVTSPNMNFPNESMDLIFSNWLLMYLSDQEVED 203
>At5g57300.1 68418.m07158 UbiE/COQ5 methyltransferase family protein
similar to ubiquinone biosynthesis methyltransferase
COQ5 [Saccharomyces cerevisiae][SP|P49017],
ubiquinone/menaquinone biosynthesis methyltransferase
ubiE [Escherichia coli][SP|P27851]; contains Pfam
profile PF01209: methlytransferase, UbiE/COQ5 family
Length = 288
Score = 32.7 bits (71), Expect = 0.22
Identities = 23/79 (29%), Positives = 37/79 (46%)
Frame = +3
Query: 369 MKNVNKARTNMKFMCMDATRMSFEDEAFNVVLDKGTLDALMPDATEETNAIIDKYFSEIX 548
M NV K R + + + + + E +A + D ++D I+K +E
Sbjct: 135 MLNVGKQRAAERGLRDNKSLVWVEGDAEALSFDDNSMDGYTIAFGIRNVTHIEKALAEAY 194
Query: 549 RVLXLGGRFVCISLLQSHI 605
RVL GGRF+C+ L SH+
Sbjct: 195 RVLKRGGRFLCLEL--SHV 211
>At3g18000.1 68416.m02288 phosphoethanolamine N-methyltransferase 1
/ PEAMT 1 (NMT1) identical to Phosphoethanolamine
N-methyltransferase 1 (EC 2.1.1.103) (PEAMT 1) (AtNMT1)
(SP:Q9FR44){Arabidopsis thaliana}; strong similarity to
phosphoethanolamine N-methyltransferase from [Spinacia
oleracea] GI:7407189, [Triticum aestivum] GI:17887465;
contains Pfam profile PF01209: methlytransferase,
UbiE/COQ5 family
Length = 491
Score = 29.9 bits (64), Expect = 1.6
Identities = 18/55 (32%), Positives = 28/55 (50%)
Frame = +3
Query: 261 SILITGCGNSSLSADLYDVGYVNITNVDVSEVVIKQMKNVNKARTNMKFMCMDAT 425
S+L G G + +L I +D + VIK+ +++N N+KFMC D T
Sbjct: 56 SVLELGAGIGRFTGELAQKAGELIA-LDFIDNVIKKNESINGHYKNVKFMCADVT 109
>At1g48600.2 68414.m05434 phosphoethanolamine N-methyltransferase 2,
putative (NMT2) very similar to |PEM2_ARATH Putative
phosphoethanolamine N-methyltransferase 2 (EC 2.1.1.103)
(SP:Q944H0){Arabidopsis thaliana}; very similar to
Halotolerance protein Hal3b (SP:P94063)[Arabidopsis
thaliana]; to similar to GB:AAF61950 from [Spinacia
oleracea]
Length = 491
Score = 29.1 bits (62), Expect = 2.7
Identities = 17/55 (30%), Positives = 27/55 (49%)
Frame = +3
Query: 261 SILITGCGNSSLSADLYDVGYVNITNVDVSEVVIKQMKNVNKARTNMKFMCMDAT 425
S+L G G + +L + +D E I++ ++VN N+KFMC D T
Sbjct: 56 SVLELGAGIGRFTGELAQKAG-EVIALDFIESAIQKNESVNGHYKNIKFMCADVT 109
>At1g48600.1 68414.m05435 phosphoethanolamine N-methyltransferase 2,
putative (NMT2) very similar to |PEM2_ARATH Putative
phosphoethanolamine N-methyltransferase 2 (EC 2.1.1.103)
(SP:Q944H0){Arabidopsis thaliana}; very similar to
Halotolerance protein Hal3b (SP:P94063)[Arabidopsis
thaliana]; to similar to GB:AAF61950 from [Spinacia
oleracea]
Length = 475
Score = 29.1 bits (62), Expect = 2.7
Identities = 17/55 (30%), Positives = 27/55 (49%)
Frame = +3
Query: 261 SILITGCGNSSLSADLYDVGYVNITNVDVSEVVIKQMKNVNKARTNMKFMCMDAT 425
S+L G G + +L + +D E I++ ++VN N+KFMC D T
Sbjct: 40 SVLELGAGIGRFTGELAQKAG-EVIALDFIESAIQKNESVNGHYKNIKFMCADVT 93
>At4g20470.1 68417.m02986 hypothetical protein
Length = 140
Score = 28.7 bits (61), Expect = 3.6
Identities = 19/62 (30%), Positives = 30/62 (48%), Gaps = 2/62 (3%)
Frame = -2
Query: 415 MHINFILVLALFTFFICLITTS--ETSTFVIFTYPTSYRSALKLELPQPVISIESLVFIY 242
+H F+L+ +L TFF +S S F F+ + RS L L P SI ++ ++
Sbjct: 50 LHCTFLLLSSLLTFFFFFRFSSIFSLSLFFSFSQKSLLRSCLSLNNVAP-FSISTITQLF 108
Query: 241 LF 236
F
Sbjct: 109 FF 110
>At5g43890.1 68418.m05367 flavin-containing monooxygenase family
protein / FMO family protein similar to
flavin-containing monooxygenases YUCCA [gi:16555352],
YUCCA2 [gi:16555354], and YUCCA3 [gi:16555356] from
Arabidopsis thaliana; contains Pfam profile PF00743
Length = 424
Score = 27.9 bits (59), Expect = 6.3
Identities = 19/74 (25%), Positives = 32/74 (43%), Gaps = 1/74 (1%)
Frame = +3
Query: 198 EWYGEYLELCGQLNKYIKTKDSILITGCGNSSLSADLYDVGY-VNITNVDVSEVVIKQMK 374
E+ GE + C + S+L+ GCGNS + L + N + V S V + +
Sbjct: 173 EFEGEVIHSCEYKSGEKYRGKSVLVVGCGNSGMEVSLDLANHNANASMVVRSSVHVLPRE 232
Query: 375 NVNKARTNMKFMCM 416
+ K+ + M M
Sbjct: 233 ILGKSSFEISMMLM 246
>At4g19440.1 68417.m02860 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 825
Score = 27.9 bits (59), Expect = 6.3
Identities = 15/51 (29%), Positives = 27/51 (52%)
Frame = +3
Query: 228 GQLNKYIKTKDSILITGCGNSSLSADLYDVGYVNITNVDVSEVVIKQMKNV 380
G LNK I+ KD ++ G +S + + GY D +E ++K+M ++
Sbjct: 379 GSLNKAIEIKDLMVSKGLSLTSSTYNTLIKGYCKNGQADNAERLLKEMLSI 429
>At3g48290.1 68416.m05270 cytochrome P450, putative very strong
similarity to Cytochrome P450 71A24
(SP:Q9STK9)[Arabidopsis thaliana];
Length = 488
Score = 27.9 bits (59), Expect = 6.3
Identities = 12/27 (44%), Positives = 15/27 (55%)
Frame = -3
Query: 357 QLPRHPHLLYLHIQHRIGPRSNLNYHS 277
QL RHPH + HR GP L++ S
Sbjct: 48 QLGRHPHRSLCSLSHRYGPLMLLHFGS 74
>At2g34030.1 68415.m04166 calcium-binding EF hand family protein
contains INTERPRO:IPR002048 calcium-binding EF-hand
domain
Length = 566
Score = 27.5 bits (58), Expect = 8.3
Identities = 14/35 (40%), Positives = 20/35 (57%)
Frame = -2
Query: 529 LSIMAFVSSVASGIRASRVPLSNTTLNASSSKDIL 425
LSI +S V+S + S VPL+N+ L + D L
Sbjct: 11 LSIALIISGVSSRVLISHVPLNNSILISDGIHDAL 45
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,322,439
Number of Sequences: 28952
Number of extensions: 226499
Number of successful extensions: 693
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 669
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 687
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1373722560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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