BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fprWP01_F_D03 (348 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC31A2.08 |mrp20||mitochondrial ribosomal protein subunit L23|... 44 9e-06 SPBC4F6.04 |rpl2502|rpl25b, rpl23a-2|60S ribosomal protein L25|S... 36 0.002 SPBC106.18 |rpl2501|rpl25a|60S ribosomal protein L25|Schizosacch... 32 0.022 SPAC694.06c |mrc1||mediator of replication checkpoint 1 |Schizos... 26 1.9 SPAC22E12.05c |rer1||Rer1 family protein|Schizosaccharomyces pom... 25 3.4 SPBC691.03c |apl3||AP-2 adaptor complex subunit Alp3 |Schizosacc... 25 3.4 SPBC4.03c |||COPII-coated vesicle component Sfb3 |Schizosaccharo... 25 4.4 SPAC2G11.10c |||URM1 activating enzyme |Schizosaccharomyces pomb... 24 7.8 SPAC3A11.14c |pkl1|klp1, SPAC3H5.03c|kinesin-like protein Pkl1|S... 24 7.8 SPAC6F6.13c |||DUF726 family protein|Schizosaccharomyces pombe|c... 24 7.8 >SPAC31A2.08 |mrp20||mitochondrial ribosomal protein subunit L23|Schizosaccharomyces pombe|chr 1|||Manual Length = 161 Score = 43.6 bits (98), Expect = 9e-06 Identities = 26/91 (28%), Positives = 46/91 (50%) Frame = +1 Query: 19 VFLPNFWMKLVRPHPKQLPNIVHFHCSMEMTKYDIKNYLEKIYEVPVVDVRTKINMGKFK 198 V+ PN L R Q P F + + K+D+++YL+ +Y + VV V+++I GK Sbjct: 10 VYFPNITFTLCRGLNLQ-PKFAVFRVPLHVNKFDVRDYLQHVYNLKVVSVKSRIYQGKLF 68 Query: 199 KDVVKGYVIKEDDVKVAFVTLPKTMTFKYPD 291 ++ + G V + K V + + F YP+ Sbjct: 69 RNQL-GQVKRPQSEKRVIVEMEE--PFVYPN 96 >SPBC4F6.04 |rpl2502|rpl25b, rpl23a-2|60S ribosomal protein L25|Schizosaccharomyces pombe|chr 2|||Manual Length = 141 Score = 35.9 bits (79), Expect = 0.002 Identities = 17/46 (36%), Positives = 25/46 (54%) Frame = +1 Query: 76 NIVHFHCSMEMTKYDIKNYLEKIYEVPVVDVRTKINMGKFKKDVVK 213 N + FH ++ K+ IKN ++K+Y V V + T I KK VK Sbjct: 79 NTLVFHVHLKANKFTIKNAVKKLYSVDAVKINTLIRPNGTKKAFVK 124 >SPBC106.18 |rpl2501|rpl25a|60S ribosomal protein L25|Schizosaccharomyces pombe|chr 2|||Manual Length = 141 Score = 32.3 bits (70), Expect = 0.022 Identities = 16/46 (34%), Positives = 23/46 (50%) Frame = +1 Query: 76 NIVHFHCSMEMTKYDIKNYLEKIYEVPVVDVRTKINMGKFKKDVVK 213 N + FH ++ K+ IK + K+Y V V + T I KK VK Sbjct: 79 NTLVFHVHLKANKFTIKEAVRKLYSVEPVKINTLIRPNGTKKAFVK 124 >SPAC694.06c |mrc1||mediator of replication checkpoint 1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1019 Score = 25.8 bits (54), Expect = 1.9 Identities = 18/84 (21%), Positives = 37/84 (44%), Gaps = 7/84 (8%) Frame = +1 Query: 97 SMEMTKYDIKNYLEKIYEVPVVDVRTKINMGKFKKDVVKGYVIK-------EDDVKVAFV 255 S+E ++K + ++ + T +N + KK+ K +++K EDD+ Sbjct: 297 SVEDDSLELKEITPEALDIGQTSLFTTLNQTQVKKEDNKKFLLKEINAKLNEDDIDSELE 356 Query: 256 TLPKTMTFKYPDLFEKSINEEEEH 327 K T ++ + ++EE EH Sbjct: 357 IEVKPKTTALDNIEKSKLSEENEH 380 >SPAC22E12.05c |rer1||Rer1 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 184 Score = 25.0 bits (52), Expect = 3.4 Identities = 11/36 (30%), Positives = 20/36 (55%) Frame = +1 Query: 52 RPHPKQLPNIVHFHCSMEMTKYDIKNYLEKIYEVPV 159 RP ++LP ++ SM T + + +I++VPV Sbjct: 107 RPFIRRLPEFKFWYSSMRATLFALVASFFRIFDVPV 142 >SPBC691.03c |apl3||AP-2 adaptor complex subunit Alp3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 878 Score = 25.0 bits (52), Expect = 3.4 Identities = 11/42 (26%), Positives = 22/42 (52%) Frame = +1 Query: 28 PNFWMKLVRPHPKQLPNIVHFHCSMEMTKYDIKNYLEKIYEV 153 P+ + LV +P+ +PN+ +++ +YDI L Y + Sbjct: 605 PSSKVNLVDTYPQTIPNVSKPSTPIDVPEYDISACLPGFYRL 646 >SPBC4.03c |||COPII-coated vesicle component Sfb3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 891 Score = 24.6 bits (51), Expect = 4.4 Identities = 13/30 (43%), Positives = 17/30 (56%) Frame = +1 Query: 160 VDVRTKINMGKFKKDVVKGYVIKEDDVKVA 249 V+ R ++N+G V K Y IKED K A Sbjct: 263 VESRPELNVGTIDFKVGKDYWIKEDPPKPA 292 >SPAC2G11.10c |||URM1 activating enzyme |Schizosaccharomyces pombe|chr 1|||Manual Length = 401 Score = 23.8 bits (49), Expect = 7.8 Identities = 11/40 (27%), Positives = 19/40 (47%) Frame = +1 Query: 118 DIKNYLEKIYEVPVVDVRTKINMGKFKKDVVKGYVIKEDD 237 D+KN +E + +DVR + G + + K + E D Sbjct: 306 DLKNLIETSPHITFLDVREPVQFGICRLPLFKNIPLSEVD 345 >SPAC3A11.14c |pkl1|klp1, SPAC3H5.03c|kinesin-like protein Pkl1|Schizosaccharomyces pombe|chr 1|||Manual Length = 832 Score = 23.8 bits (49), Expect = 7.8 Identities = 12/46 (26%), Positives = 27/46 (58%), Gaps = 1/46 (2%) Frame = +1 Query: 100 MEMTKYDI-KNYLEKIYEVPVVDVRTKINMGKFKKDVVKGYVIKED 234 M + +Y+ K+Y EK YE + +R ++ + D+++ ++KE+ Sbjct: 362 MAVKEYESEKSYSEKEYEEKISSLRIELEDKLAEIDMLRNKLLKEE 407 >SPAC6F6.13c |||DUF726 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 778 Score = 23.8 bits (49), Expect = 7.8 Identities = 10/19 (52%), Positives = 16/19 (84%), Gaps = 1/19 (5%) Frame = +1 Query: 208 VKGYVI-KEDDVKVAFVTL 261 V G+++ KEDDV+++F TL Sbjct: 455 VSGWMLSKEDDVRLSFATL 473 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,228,036 Number of Sequences: 5004 Number of extensions: 20442 Number of successful extensions: 66 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 66 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 66 length of database: 2,362,478 effective HSP length: 64 effective length of database: 2,042,222 effective search space used: 104153322 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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