Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fprWP01_F_D03
(348 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g33330.1 68417.m04740 glycogenin glucosyltransferase (glycoge... 28 2.0
At1g48920.1 68414.m05480 nucleolin, putative similar to nuM1 pro... 27 2.6
At1g78210.1 68414.m09114 hydrolase, alpha/beta fold family prote... 27 3.4
At3g11800.1 68416.m01446 expressed protein 27 4.5
At4g18330.2 68417.m02719 eukaryotic translation initiation facto... 26 6.0
At4g18330.1 68417.m02718 eukaryotic translation initiation facto... 26 6.0
At3g62120.2 68416.m06980 tRNA synthetase class II (G, H, P and S... 26 6.0
At3g62120.1 68416.m06979 tRNA synthetase class II (G, H, P and S... 26 6.0
At2g34220.1 68415.m04187 hypothetical protein contains Pfam prof... 26 6.0
At4g35920.3 68417.m05107 expressed protein contains Pfam profile... 26 7.9
At4g35920.2 68417.m05106 expressed protein contains Pfam profile... 26 7.9
At4g35920.1 68417.m05105 expressed protein contains Pfam profile... 26 7.9
>At4g33330.1 68417.m04740 glycogenin glucosyltransferase
(glycogenin)-related similar to glycogenin
glucosyltransferase (glycogenin-1) (EC 2.4.1.186) from
Homo sapiens [SP|P46976], Mus musculus [SP|Q9R062],
Oryctolagus cuniculus [GI:165513]; contains Pfam profile
PF01501: Glycosyl transferase family 8
Length = 593
Score = 27.9 bits (59), Expect = 2.0
Identities = 21/72 (29%), Positives = 38/72 (52%)
Frame = +1
Query: 109 TKYDIKNYLEKIYEVPVVDVRTKINMGKFKKDVVKGYVIKEDDVKVAFVTLPKTMTFKYP 288
TK + +++E+I + +TKI M ++ + + + V + F + K FK+
Sbjct: 87 TKLEAPSFMEEILTRGLG--KTKIGMVNMEECDLTNWKRYGETVHIHFERVSKL--FKWQ 142
Query: 289 DLFEKSINEEEE 324
DLF + I+EEEE
Sbjct: 143 DLFPEWIDEEEE 154
>At1g48920.1 68414.m05480 nucleolin, putative similar to nuM1
protein GI:1279562 from [Medicago sativa]
Length = 557
Score = 27.5 bits (58), Expect = 2.6
Identities = 25/69 (36%), Positives = 35/69 (50%)
Frame = +1
Query: 133 LEKIYEVPVVDVRTKINMGKFKKDVVKGYVIKEDDVKVAFVTLPKTMTFKYPDLFEKSIN 312
L+K P D+ TK+++ K KKDV+ V KE VK +PK + S +
Sbjct: 20 LKKGKREPEDDIDTKVSLKKQKKDVIAA-VQKEKAVK----KVPKKVESSDD---SDSES 71
Query: 313 EEEEHAKSL 339
EEEE AK +
Sbjct: 72 EEEEKAKKV 80
>At1g78210.1 68414.m09114 hydrolase, alpha/beta fold family protein
low similarity to hydrolases from Rhodococcus sp. EtbD2
GI:3273241, EtbD1 GI:3273239; contains Pfam profile
PF00561: hydrolase, alpha/beta fold family
Length = 314
Score = 27.1 bits (57), Expect = 3.4
Identities = 12/49 (24%), Positives = 23/49 (46%), Gaps = 3/49 (6%)
Frame = +1
Query: 31 NFWMKLVRPHPKQLPNIVHFH---CSMEMTKYDIKNYLEKIYEVPVVDV 168
NFW+ +P K PN++ H + YD+ L + + + + D+
Sbjct: 38 NFWVSKTKPESKPKPNLLLIHGLGATAIWQWYDVARRLSRYFNLYIPDL 86
>At3g11800.1 68416.m01446 expressed protein
Length = 246
Score = 26.6 bits (56), Expect = 4.5
Identities = 15/60 (25%), Positives = 29/60 (48%)
Frame = +1
Query: 85 HFHCSMEMTKYDIKNYLEKIYEVPVVDVRTKINMGKFKKDVVKGYVIKEDDVKVAFVTLP 264
H H S+ ++ + K+ E+ ++ + T + F+ D KGY++ K A +LP
Sbjct: 71 HRHLSLNGNS-EVAVFRPKVDEITLLTINTS-SSSSFRPDASKGYMVAFAGAKYAARSLP 128
>At4g18330.2 68417.m02719 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 471
Score = 26.2 bits (55), Expect = 6.0
Identities = 15/50 (30%), Positives = 28/50 (56%), Gaps = 1/50 (2%)
Frame = +1
Query: 148 EVPVVDVRTKINMGKFKKDVVKGYVIKEDDVKVA-FVTLPKTMTFKYPDL 294
+ P+V V ++ K+ DVV Y++K+ + V FV+ PK + + D+
Sbjct: 217 DAPIVPVSAQL---KYNIDVVSEYIVKKIPIPVRDFVSPPKMIVIRSFDV 263
>At4g18330.1 68417.m02718 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 284
Score = 26.2 bits (55), Expect = 6.0
Identities = 15/50 (30%), Positives = 28/50 (56%), Gaps = 1/50 (2%)
Frame = +1
Query: 148 EVPVVDVRTKINMGKFKKDVVKGYVIKEDDVKVA-FVTLPKTMTFKYPDL 294
+ P+V V ++ K+ DVV Y++K+ + V FV+ PK + + D+
Sbjct: 217 DAPIVPVSAQL---KYNIDVVSEYIVKKIPIPVRDFVSPPKMIVIRSFDV 263
>At3g62120.2 68416.m06980 tRNA synthetase class II (G, H, P and S)
family protein similar to SP|P07814 Bifunctional
aminoacyl-tRNA synthetase [Includes: Glutamyl-tRNA
synthetase (EC 6.1.1.17) (Glutamate--tRNA ligase);
Prolyl-tRNA synthetase (EC 6.1.1.15) (Proline--tRNA
ligase)] {Homo sapiens}; contains Pfam profiles PF00587:
tRNA synthetase class II core domain (G, H, P, S and T),
PF03129: Anticodon binding domain
Length = 530
Score = 26.2 bits (55), Expect = 6.0
Identities = 18/81 (22%), Positives = 34/81 (41%)
Frame = +1
Query: 46 LVRPHPKQLPNIVHFHCSMEMTKYDIKNYLEKIYEVPVVDVRTKINMGKFKKDVVKGYVI 225
++RP + I+ E+ K +KN ++ P V + K ++ F +V
Sbjct: 81 ILRPWSMAIWEIMQIFFDAEIKKMKVKNCYFPLFVSPGVLEKEKDHIEGFAPEVAWVTKS 140
Query: 226 KEDDVKVAFVTLPKTMTFKYP 288
+ D++V P + T YP
Sbjct: 141 GKSDLEVPIAIRPTSETVMYP 161
>At3g62120.1 68416.m06979 tRNA synthetase class II (G, H, P and S)
family protein similar to SP|P07814 Bifunctional
aminoacyl-tRNA synthetase [Includes: Glutamyl-tRNA
synthetase (EC 6.1.1.17) (Glutamate--tRNA ligase);
Prolyl-tRNA synthetase (EC 6.1.1.15) (Proline--tRNA
ligase)] {Homo sapiens}; contains Pfam profiles PF00587:
tRNA synthetase class II core domain (G, H, P, S and T),
PF03129: Anticodon binding domain
Length = 530
Score = 26.2 bits (55), Expect = 6.0
Identities = 18/81 (22%), Positives = 34/81 (41%)
Frame = +1
Query: 46 LVRPHPKQLPNIVHFHCSMEMTKYDIKNYLEKIYEVPVVDVRTKINMGKFKKDVVKGYVI 225
++RP + I+ E+ K +KN ++ P V + K ++ F +V
Sbjct: 81 ILRPWSMAIWEIMQIFFDAEIKKMKVKNCYFPLFVSPGVLEKEKDHIEGFAPEVAWVTKS 140
Query: 226 KEDDVKVAFVTLPKTMTFKYP 288
+ D++V P + T YP
Sbjct: 141 GKSDLEVPIAIRPTSETVMYP 161
>At2g34220.1 68415.m04187 hypothetical protein contains Pfam
profiles PF04780: Protein of unknown function (DUF629),
PF04781: Protein of unknown function (DUF627)
Length = 718
Score = 26.2 bits (55), Expect = 6.0
Identities = 11/37 (29%), Positives = 21/37 (56%)
Frame = +1
Query: 223 IKEDDVKVAFVTLPKTMTFKYPDLFEKSINEEEEHAK 333
+ DDVK + +TL K++ K+P + K + + + K
Sbjct: 627 VPNDDVKKSLLTLRKSLKEKFPLIDSKILQNKSTYEK 663
>At4g35920.3 68417.m05107 expressed protein contains Pfam profile
PF04749: Protein of unknown function, DUF614; isoform
contains AT-acceptor splice site at intron 8
Length = 421
Score = 25.8 bits (54), Expect = 7.9
Identities = 12/40 (30%), Positives = 24/40 (60%), Gaps = 1/40 (2%)
Frame = +1
Query: 208 VKGYVIKEDDVKVAFVTLPKTMTFKYPDL-FEKSINEEEE 324
V+ ++K++ + A L KT++ YP+L F +++ E E
Sbjct: 159 VQDVILKQESTREAASVLKKTLSCSYPNLRFCEALKTENE 198
>At4g35920.2 68417.m05106 expressed protein contains Pfam profile
PF04749: Protein of unknown function, DUF614; isoform
contains AT-acceptor splice site at intron 8
Length = 421
Score = 25.8 bits (54), Expect = 7.9
Identities = 12/40 (30%), Positives = 24/40 (60%), Gaps = 1/40 (2%)
Frame = +1
Query: 208 VKGYVIKEDDVKVAFVTLPKTMTFKYPDL-FEKSINEEEE 324
V+ ++K++ + A L KT++ YP+L F +++ E E
Sbjct: 159 VQDVILKQESTREAASVLKKTLSCSYPNLRFCEALKTENE 198
>At4g35920.1 68417.m05105 expressed protein contains Pfam profile
PF04749: Protein of unknown function, DUF614; isoform
contains AT-acceptor splice site at intron 8
Length = 421
Score = 25.8 bits (54), Expect = 7.9
Identities = 12/40 (30%), Positives = 24/40 (60%), Gaps = 1/40 (2%)
Frame = +1
Query: 208 VKGYVIKEDDVKVAFVTLPKTMTFKYPDL-FEKSINEEEE 324
V+ ++K++ + A L KT++ YP+L F +++ E E
Sbjct: 159 VQDVILKQESTREAASVLKKTLSCSYPNLRFCEALKTENE 198
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 6,222,342
Number of Sequences: 28952
Number of extensions: 100889
Number of successful extensions: 228
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 227
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 228
length of database: 12,070,560
effective HSP length: 72
effective length of database: 9,986,016
effective search space used: 429398688
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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