Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fner13d16f
(663 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC354.02c |sec61||translocon alpha subunit Sec61|Schizosacchar... 256 2e-69
SPBC19G7.17 ||SPBC36B7.01|translocon subunit Sec61 homolog |Schi... 128 5e-31
SPAC589.05c |||conserved eukaryotic protein|Schizosaccharomyces ... 30 0.34
SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr... 29 0.79
SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|S... 28 1.4
SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 28 1.4
SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pomb... 27 1.8
SPBC1711.08 |||chaperone activator Aha1 |Schizosaccharomyces pom... 27 1.8
SPBC32H8.13c |mok12||alpha-1,3-glucan synthase Mok12|Schizosacch... 27 2.4
SPAC458.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 26 4.2
SPAC23C4.18c |rad4|cut5, dre3|BRCT domain protein Rad4|Schizosac... 26 5.6
SPCC663.15c |||conserved fungal protein|Schizosaccharomyces pomb... 26 5.6
SPCC1393.07c |mug4||sequence orphan|Schizosaccharomyces pombe|ch... 25 7.4
SPCC736.14 |dis1||microtubule-associated protein Dis1 |Schizosac... 22 8.8
SPCC622.15c |||sequence orphan|Schizosaccharomyces pombe|chr 3||... 25 9.7
SPAC17D4.03c |||membrane transporter |Schizosaccharomyces pombe|... 25 9.7
SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subuni... 25 9.7
>SPBC354.02c |sec61||translocon alpha subunit
Sec61|Schizosaccharomyces pombe|chr 2|||Manual
Length = 479
Score = 256 bits (628), Expect = 2e-69
Identities = 116/191 (60%), Positives = 154/191 (80%), Gaps = 1/191 (0%)
Frame = +1
Query: 91 IKFLEVIKPFCSILPEIAKPERKIQFREKVLWTAITLFIFLVCCQIPLFGIMSSDSADPF 270
++FL+++KPF LPEIA PERK+ F++K+LWT +TL IFLV Q+PL+GI+SSDS+DP
Sbjct: 4 LRFLDLVKPFAPFLPEIAAPERKVPFKQKMLWTGVTLLIFLVMSQVPLYGIVSSDSSDPL 63
Query: 271 YWIRVILASNRGTLMELGISPIVTSGLIMQLLAGAKIIEVG-DTPKDRALFNGAQKLFGM 447
W+R+ILA+NRGTLMELGISPIVTS +++QLL G+++IEV + DR ++ QK +
Sbjct: 64 LWLRMILAANRGTLMELGISPIVTSSMLVQLLVGSQLIEVNMELKSDREMYQLVQKFLAI 123
Query: 448 VITVGQAIVYVMTGMYGEPSEIGAGVCLLIIIQLFVAGLIVLLLDELLQKGYGLGSGISL 627
+I GQA YV+TGMYG P ++GAG+CLL+I+QL A LIVLLLDELLQKGYGLGSGISL
Sbjct: 124 IIAFGQATAYVLTGMYGRPQDLGAGICLLLILQLAAASLIVLLLDELLQKGYGLGSGISL 183
Query: 628 FIATTSVKPLY 660
FIAT + + ++
Sbjct: 184 FIATINCENIF 194
>SPBC19G7.17 ||SPBC36B7.01|translocon subunit Sec61 homolog
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 475
Score = 128 bits (310), Expect = 5e-31
Identities = 64/185 (34%), Positives = 111/185 (60%), Gaps = 1/185 (0%)
Frame = +1
Query: 88 GIKFLEVIKPFCSILPEIAKPERKIQFREKVLWTAITLFIFLVCCQIPLFGIMSSDSADP 267
G +F+ IKP S+LPE+ P+ ++ EK+ W A + ++ + IP++G +D+ DP
Sbjct: 3 GARFINFIKPLSSLLPEVEGPKTHLELVEKLGWMAGCVVVYQILSIIPVYGAEKTDTLDP 62
Query: 268 FYWIRVILASNRGTLMELGISPIVTSGLIMQLLAGAKIIEVG-DTPKDRALFNGAQKLFG 444
RV+ S+ LM G++PI S ++Q+LA K I V + DR LF AQK+
Sbjct: 63 INNFRVLDGSSASGLMITGLAPIYLSSFLLQILASKKKIAVNFNLIIDRVLFQNAQKVVS 122
Query: 445 MVITVGQAIVYVMTGMYGEPSEIGAGVCLLIIIQLFVAGLIVLLLDELLQKGYGLGSGIS 624
++ + A+ YV +G YG S++G +++I+Q+F+ G++ + L E+++KG+GLGSG
Sbjct: 123 ALLYLILAVTYVSSGYYGSFSDLGIFRFIMLILQIFLPGIVCIYLCEIIEKGHGLGSGPV 182
Query: 625 LFIAT 639
L + +
Sbjct: 183 LLLGS 187
>SPAC589.05c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 346
Score = 29.9 bits (64), Expect = 0.34
Identities = 23/67 (34%), Positives = 30/67 (44%), Gaps = 4/67 (5%)
Frame = -2
Query: 437 NFCAPLNKALSFGVSPTS-MILAPASNCMISPDVTIGEIPSSINV---PLFDARITRIQ* 270
+ CA +NKAL G+ TS A C PD I + S V PL + RI ++
Sbjct: 104 SLCAAINKALDAGIPITSPAFYADEKQC---PDTLIASVFDSATVEKIPLLEERIRIMRA 160
Query: 269 KGSALSD 249
G L D
Sbjct: 161 SGRVLVD 167
>SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 780
Score = 28.7 bits (61), Expect = 0.79
Identities = 14/43 (32%), Positives = 25/43 (58%)
Frame = -2
Query: 635 AINREIPEPNPYPFWSNSSSNNTIRPATNSCMIMRRHTPAPIS 507
+I+RE+ N PF SNS S ++ T++ +R + +PI+
Sbjct: 31 SISRELDYANQSPFSSNSFSPTELKARTSATFDVRSASTSPIN 73
>SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase
Mok11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2397
Score = 27.9 bits (59), Expect = 1.4
Identities = 17/66 (25%), Positives = 31/66 (46%), Gaps = 3/66 (4%)
Frame = -1
Query: 627 QRDTRTQSISFLEQ---FIEQQHNKTGHKQLYDNEEAHSGTNFTGFTIHSSHYINNSLTN 457
Q+ +S S ++ F E Q++ TG+K + A +G + G + +S +
Sbjct: 1700 QKSPSVESFSIIDNDNPFHEGQNSSTGYKDIVQGLLAENGVSEAGVDVMTSIVSSTIPIV 1759
Query: 456 SNHHTK 439
SNH T+
Sbjct: 1760 SNHQTE 1765
>SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 676
Score = 27.9 bits (59), Expect = 1.4
Identities = 11/21 (52%), Positives = 14/21 (66%)
Frame = -2
Query: 629 NREIPEPNPYPFWSNSSSNNT 567
N +P P P P +NSS+NNT
Sbjct: 395 NSNMPPPAPRPVTNNSSNNNT 415
>SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1334
Score = 27.5 bits (58), Expect = 1.8
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = -1
Query: 570 HNKTGHKQLYDNEEAHSGTNFTGFTIHSSHYINNSLTNSNH 448
+NK HK+L DNE SGT + T+ + + N T H
Sbjct: 515 NNKNHHKELEDNE---SGTRISPSTLGDTSFPNGIFTLLTH 552
>SPBC1711.08 |||chaperone activator Aha1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 336
Score = 27.5 bits (58), Expect = 1.8
Identities = 16/42 (38%), Positives = 22/42 (52%), Gaps = 1/42 (2%)
Frame = -1
Query: 564 KTGHKQLYDNEEAHSGTNFTGFTIHSSHYINN-SLTNSNHHT 442
+T +Y + E H+G G +HSS NN S T+SN T
Sbjct: 144 QTHGDDVYLSTEEHNGNAARGLPVHSSFKQNNSSQTSSNKGT 185
>SPBC32H8.13c |mok12||alpha-1,3-glucan synthase
Mok12|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2352
Score = 27.1 bits (57), Expect = 2.4
Identities = 18/54 (33%), Positives = 27/54 (50%), Gaps = 1/54 (1%)
Frame = +1
Query: 91 IKFLEVI-KPFCSILPEIAKPERKIQFREKVLWTAITLFIFLVCCQIPLFGIMS 249
I+FL+ KP+ S + R++ + K WT + I L+ PLFGI S
Sbjct: 1055 IRFLDPPPKPYLSYKMYLNDFTRQLHYIPKGSWTVQIVAIVLLILLPPLFGIFS 1108
>SPAC458.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 340
Score = 26.2 bits (55), Expect = 4.2
Identities = 17/61 (27%), Positives = 29/61 (47%)
Frame = -2
Query: 317 SINVPLFDARITRIQ*KGSALSDDIIPNKGIWQHTKKINNVIAVHNTFSRN*ILRSGFAI 138
S+ + LFD I+ + S +SD IP K +++ N + T+S N + +S
Sbjct: 273 SLLLGLFDKSISMLNCHSSKISDGDIPYKSHQVFLQELQNKYSEIKTYSSNKLNKSSIKS 332
Query: 137 S 135
S
Sbjct: 333 S 333
>SPAC23C4.18c |rad4|cut5, dre3|BRCT domain protein
Rad4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 648
Score = 25.8 bits (54), Expect = 5.6
Identities = 9/23 (39%), Positives = 14/23 (60%)
Frame = -2
Query: 140 ISGNILQNGLITSKNFIPILIYC 72
+ G L +GL+ K+F+P L C
Sbjct: 83 VQGEDLDDGLLVDKHFLPTLFKC 105
>SPCC663.15c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 657
Score = 25.8 bits (54), Expect = 5.6
Identities = 17/62 (27%), Positives = 30/62 (48%), Gaps = 8/62 (12%)
Frame = -1
Query: 663 SIQWFHRCCSNKQRDTRTQS--------ISFLEQFIEQQHNKTGHKQLYDNEEAHSGTNF 508
++QWF + +Q D R+QS I+ LE + E Q +T + D E++ ++
Sbjct: 321 ALQWFTQLSRTEQDDVRSQSINKELNITIAILEAY-EAQQTETTETEGVDKEDSDKASSV 379
Query: 507 TG 502
G
Sbjct: 380 QG 381
>SPCC1393.07c |mug4||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 845
Score = 25.4 bits (53), Expect = 7.4
Identities = 15/56 (26%), Positives = 28/56 (50%)
Frame = -2
Query: 440 NNFCAPLNKALSFGVSPTSMILAPASNCMISPDVTIGEIPSSINVPLFDARITRIQ 273
N C +K + P ++I +P+S S ++ + PSS+NV + R+ +Q
Sbjct: 368 NGLCRNDSKCRFLMILPETIIKSPSSFIGDSYNIANIDPPSSMNVQSSEFRVGEVQ 423
>SPCC736.14 |dis1||microtubule-associated protein Dis1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 882
Score = 21.8 bits (44), Expect(2) = 8.8
Identities = 10/34 (29%), Positives = 17/34 (50%)
Frame = -2
Query: 614 EPNPYPFWSNSSSNNTIRPATNSCMIMRRHTPAP 513
+P P+ +SS NT++ +T+S R P
Sbjct: 254 KPKPHLSSKSSSQGNTLQRSTSSFSTPNRKVSQP 287
Score = 21.4 bits (43), Expect(2) = 8.8
Identities = 9/24 (37%), Positives = 14/24 (58%)
Frame = -2
Query: 404 FGVSPTSMILAPASNCMISPDVTI 333
F SP+ I++PA N + S V +
Sbjct: 290 FSASPSRSIVSPAKNIVGSTPVDV 313
>SPCC622.15c |||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 557
Score = 25.0 bits (52), Expect = 9.7
Identities = 11/21 (52%), Positives = 15/21 (71%)
Frame = -2
Query: 353 ISPDVTIGEIPSSINVPLFDA 291
IS ++T GE+PS NVP +A
Sbjct: 414 ISANMTTGEMPSMTNVPAGNA 434
>SPAC17D4.03c |||membrane transporter |Schizosaccharomyces pombe|chr
1|||Manual
Length = 732
Score = 25.0 bits (52), Expect = 9.7
Identities = 13/48 (27%), Positives = 21/48 (43%)
Frame = -1
Query: 612 TQSISFLEQFIEQQHNKTGHKQLYDNEEAHSGTNFTGFTIHSSHYINN 469
T I+ E+F E++ N K Y N++ S T H+ +N
Sbjct: 498 TNDINIFEEFEEEKDNVEAQKMGYTNDDHVSQHEHTHENSQEHHHEHN 545
>SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subunit
Cct1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 556
Score = 25.0 bits (52), Expect = 9.7
Identities = 12/44 (27%), Positives = 22/44 (50%)
Frame = -2
Query: 236 NKGIWQHTKKINNVIAVHNTFSRN*ILRSGFAISGNILQNGLIT 105
+KG ++ K N++ H SR +L G+A++ I + T
Sbjct: 189 SKGETRYPVKAVNILKAHGKSSRESVLVKGYALNCTIASQAMKT 232
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,830,096
Number of Sequences: 5004
Number of extensions: 61575
Number of successful extensions: 183
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 174
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 181
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 301829700
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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