BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fner12m14r
(712 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 49 8e-07
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 48 1e-06
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 39 6e-04
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 38 0.001
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 35 0.010
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 30 0.38
SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|c... 29 0.87
SPAC1786.03 |cut11|SPAC24C9.01|integral membrane nucleoporin|Sch... 28 1.5
SPAC27D7.03c |mei2||RNA-binding protein involved in meiosis Mei2... 27 2.0
SPAC22F3.04 |mug62||AMP binding enzyme |Schizosaccharomyces pomb... 27 2.0
SPAC24H6.01c ||SPAPB21F2.01|membrane bound O-acyltransferase, MB... 27 3.5
SPAC24B11.12c |||P-type ATPase |Schizosaccharomyces pombe|chr 1|... 26 4.6
SPBC1347.05c |||DNAJ domain protein Scj1|Schizosaccharomyces pom... 26 4.6
SPMIT.02 |||mitochondrial DNA binding endonuclease|Schizosacchar... 26 6.1
SPAP8A3.05 |||ski complex subunit Ski7 |Schizosaccharomyces pomb... 26 6.1
SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosa... 25 8.1
>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 103
Score = 48.8 bits (111), Expect = 8e-07
Identities = 19/39 (48%), Positives = 30/39 (76%)
Frame = -1
Query: 706 VKVDVDECEDIASEYNINSMPTFVFVKNGKKLDEFSGAN 590
+KVDVD+ +IA+E +++MP+F KNG+K++E GAN
Sbjct: 53 IKVDVDQLSEIAAEAGVHAMPSFFLYKNGEKIEEIVGAN 91
>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
Txl1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 290
Score = 48.4 bits (110), Expect = 1e-06
Identities = 22/41 (53%), Positives = 29/41 (70%)
Frame = -1
Query: 712 VGVKVDVDECEDIASEYNINSMPTFVFVKNGKKLDEFSGAN 590
V KV+VDE IAS + +MPTFVF +NGK++D +GAN
Sbjct: 54 VFAKVNVDEQRQIASGLGVKAMPTFVFFENGKQIDMLTGAN 94
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 39.1 bits (87), Expect = 6e-04
Identities = 17/37 (45%), Positives = 24/37 (64%)
Frame = -1
Query: 706 VKVDVDECEDIASEYNINSMPTFVFVKNGKKLDEFSG 596
V+VD E D+ SEY+I PT KNGK++ ++SG
Sbjct: 76 VEVDCTEEGDLCSEYSIRGYPTLNVFKNGKQISQYSG 112
>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 38.3 bits (85), Expect = 0.001
Identities = 15/41 (36%), Positives = 26/41 (63%)
Frame = -1
Query: 712 VGVKVDVDECEDIASEYNINSMPTFVFVKNGKKLDEFSGAN 590
V +K++ ++ DIA +++N++P FV + K L SGAN
Sbjct: 53 VFLKIEAEKFSDIAESFDVNAVPLFVLIHGAKVLARISGAN 93
>SPBC12D12.07c |trx2||mitochondrial thioredoxin
Trx2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 35.1 bits (77), Expect = 0.010
Identities = 14/40 (35%), Positives = 25/40 (62%)
Frame = -1
Query: 706 VKVDVDECEDIASEYNINSMPTFVFVKNGKKLDEFSGANV 587
+ V+ D+ DIA + + ++PT V + G++LD GA+V
Sbjct: 70 IAVNADKFSDIAQKNGVYALPTMVLFRKGQELDRIVGADV 109
>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 359
Score = 29.9 bits (64), Expect = 0.38
Identities = 13/38 (34%), Positives = 22/38 (57%), Gaps = 1/38 (2%)
Frame = -1
Query: 703 KVDVDECEDIASEYNINSMPTFV-FVKNGKKLDEFSGA 593
K+D D D+A +Y+I PT + F +G + ++S A
Sbjct: 78 KIDADTHSDVADKYHITGFPTLIWFPPDGSEPVQYSNA 115
>SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1687
Score = 28.7 bits (61), Expect = 0.87
Identities = 11/30 (36%), Positives = 17/30 (56%)
Frame = +1
Query: 433 KFITDVKNIFYSFIKNVYMFFFFNSILVSI 522
KFI D +N F+ I Y F F ++L+ +
Sbjct: 436 KFILDSQNFFFESINTEYSFIIFTNLLMHL 465
>SPAC1786.03 |cut11|SPAC24C9.01|integral membrane
nucleoporin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 601
Score = 27.9 bits (59), Expect = 1.5
Identities = 10/27 (37%), Positives = 17/27 (62%)
Frame = +2
Query: 428 SSNSLQTLKTFFIHLLKTYTCFFFLIP 508
SS +L +F+ L++ + CFFF+ P
Sbjct: 50 SSGPFISLSFWFLSLVRGFVCFFFMFP 76
>SPAC27D7.03c |mei2||RNA-binding protein involved in meiosis
Mei2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 750
Score = 27.5 bits (58), Expect = 2.0
Identities = 12/41 (29%), Positives = 22/41 (53%)
Frame = +1
Query: 409 QTQKINIFKFITDVKNIFYSFIKNVYMFFFFNSILVSIGGC 531
++ + N ++TD +NIF +F+ N + F + L I C
Sbjct: 71 KSSESNSIDYLTDTQNIFPNFVNNENNYQFSTAPLNPIDAC 111
>SPAC22F3.04 |mug62||AMP binding enzyme |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1428
Score = 27.5 bits (58), Expect = 2.0
Identities = 13/30 (43%), Positives = 18/30 (60%)
Frame = +3
Query: 201 IGEIIHLSKPDKNKNKNITIHTLKFLSNHL 290
+G I HL K D+N K ++TL F+ N L
Sbjct: 1251 LGFIHHLKKKDQNMEKVPVLYTLDFIWNTL 1280
>SPAC24H6.01c ||SPAPB21F2.01|membrane bound O-acyltransferase, MBOAT
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 588
Score = 26.6 bits (56), Expect = 3.5
Identities = 9/26 (34%), Positives = 15/26 (57%)
Frame = +2
Query: 98 CFYLHNIKKKKHCDTCYLQKFLKARW 175
CF +H + KH + ++K+LK W
Sbjct: 74 CFKIHYVSSPKHPNYKNIEKYLKPGW 99
>SPAC24B11.12c |||P-type ATPase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1402
Score = 26.2 bits (55), Expect = 4.6
Identities = 17/45 (37%), Positives = 22/45 (48%)
Frame = +1
Query: 388 FFAFQRLQTQKINIFKFITDVKNIFYSFIKNVYMFFFFNSILVSI 522
+F +++T K FI KNIF F +FF F IL SI
Sbjct: 84 WFPRNKIRTAKYTPIDFIP--KNIFLQFQNVANLFFLFLVILQSI 126
>SPBC1347.05c |||DNAJ domain protein Scj1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 381
Score = 26.2 bits (55), Expect = 4.6
Identities = 11/35 (31%), Positives = 20/35 (57%)
Frame = +3
Query: 165 KHDGNLSYTLISIGEIIHLSKPDKNKNKNITIHTL 269
K DG+ S GE++H + ++ KN+ + I+ L
Sbjct: 301 KLDGSFMEVKRSAGEVVHPGETERVKNQGMPIYNL 335
>SPMIT.02 |||mitochondrial DNA binding
endonuclease|Schizosaccharomyces pombe|chr
mitochondrial|||Manual
Length = 384
Score = 25.8 bits (54), Expect = 6.1
Identities = 14/61 (22%), Positives = 28/61 (45%)
Frame = +1
Query: 370 ITTEPIFFAFQRLQTQKINIFKFITDVKNIFYSFIKNVYMFFFFNSILVSIGGCVQL*SL 549
+ T+ I+F + T + + + +++ F S ++N+ FF S G C+ L
Sbjct: 22 VITQLIYFLTSKKITN-LGKIRLVKSIRDSFLSQLENILCFFLVYRTTYSFGVCLMKRFL 80
Query: 550 F 552
F
Sbjct: 81 F 81
>SPAP8A3.05 |||ski complex subunit Ski7 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 695
Score = 25.8 bits (54), Expect = 6.1
Identities = 18/55 (32%), Positives = 29/55 (52%), Gaps = 3/55 (5%)
Frame = +3
Query: 153 KSF*KH---DGNLSYTLISIGEIIHLSKPDKNKNKNITIHTLKFLSNHLVKKTKI 308
KSF KH D L ++ S+ ++ KP KN N + HTL + L++ +K+
Sbjct: 223 KSFQKHKRLDEQLLNSVKSMKKVSQQLKPQKNTNDSNNDHTL-LSQDQLIELSKL 276
>SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1029
Score = 25.4 bits (53), Expect = 8.1
Identities = 9/22 (40%), Positives = 16/22 (72%)
Frame = +2
Query: 419 KLISSNSLQTLKTFFIHLLKTY 484
KL+SSN+LQ + F+ ++K +
Sbjct: 412 KLVSSNTLQAMSHFYATMIKLF 433
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,757,578
Number of Sequences: 5004
Number of extensions: 57195
Number of successful extensions: 195
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 184
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 195
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 331187010
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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